Cours du 12 décembre 2011 - Collège de France · Cours du 12 décembre 2011 ... Nature...
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Cours du 12 décembre 2011
Transcript of Cours du 12 décembre 2011 - Collège de France · Cours du 12 décembre 2011 ... Nature...
Cours du 12 décembre 2011
DIA IX.12
CRITICAL PERIOD AND MATURATION OF LOCAL GABAERGIC CIRCUITS
Blue diamond: Nduf family Red triangles: Atp5 family Green dots: Cox6 family
A novel (ancient?) signaling mechanism
Central visual pathway and cri:cal period for binocular vision
Otx2 expression in PV cells parallels cri:cal period
Sugiyama et al. Cell 2008
Otx2 expression, as PV matura:on, is ac:vity‐dependent
Sugiyama et al. Cell 2008
AN OTX2 MOTIF IS NECESSARY FOR ITS BINDING TO CORTICAL CELLS
RK‐PEPTIDE INFUSION IMPAIRS ENDOGENOUS OTX2 TRANSFER INTO V1
Beurdeley, Spatazza et al., in revision
RK-PEPTIDE REOPENS PLASTICITY IN THE ADULT
> P 60 Peptide Infusion
D0 D7 D2 D6
Recording
Normal adult mouse visual acuity : ≈ 0,45 – 0,6
Beurdeley, Spatazza et al., in revision
RECOVERY FROM AMBLYOPIA
P19 P33
RK
P60 P67
Recording
P74-95
Recording
Otx2 is expressed in the choroid plexus where it can be specifically recombined
Spatazza et al. in preparaMon
Sacrifice 14 days after a single CRE-Tat injection
RECOMBINING OTX2 IN THE CHOROID PLEXUS REDUCES ITS CORTICAL CONTENT AND REDUCES PV CELL MATURATION MARKERS
RECOMBINING OTX2 IN THE ADULT PLEXUS REOPENS PLASTICITY
Otx2flox/flox mice > P60 D0 D14 D18
Recording CRE-Tat or vehicle injection
