BOCAR, Rainel JosephBaldovino, Andre

Bayle, SophiaBariata, Don Kim

Briones, KarenBungay, ElkaB1 Group 3

Comparison of Essential Fatty Acid Intakes and Serum Levels

of Inflammatory Factors between Asthmatic and Healthy Adults:

A Case- Control Study

Received: 16 November 2013; Received in revised form: 21 January 2014; Accepted: 16

February 2014

Conducted a study to compare the dietary intake of essential fatty acids (FAs) and serum levels of inflammatory factors in both asthmatic and healthy adults

It aimed to examine the correlation between inflammatory markers and FAs.

Performed using a CASE-CONTROL study on:◦ 47 ASTHMATIC patients (26 males and 21 females) VERSUS◦ 47 CONTROLS (24 males and 23 males)

Tests performed on participants:◦ TNF-alpha, high sensitive C-reactive protein (hs-CRP), leptin and

adinopectin◦ Assessment by semi-quantitative food frequency questionaire

(FFQ)

II. Abstract

Results: II. Abstract

Asthma ControlsDietary intake of omega-3 FAs

Significantly LOWER

-

Serum Concentration (TNF-alpha, hs-CRP, leptin)

SignificantlyHIGHER

-

Dietary intake of adinopectin and saturated FAs

negative negative

Correlation between other inflammatory factors and dietary intakes

NOT significant NOT significant

Higher intake of omega-3 and lower levels of inflammatory factors in the healthy control group compared to asthmatic group may explain the protective role of essential fatty acids in asthma.

General Objective◦ To compare the dietary intake of essential fatty

acids (FAs) and serum levels of inflammatory factors in both asthmatic and healthy adults

◦ Specific Objectives To examine potential relationship between

inflammatory markers and dietary fatty acids. To measure serum concentration TNF-alpha , hs-CRP ,

leptin and adiponectin of both cases and controls. To statistically analyze the data by independent t-test

and ANCOVA.

III. Objectives

IV. Materials and MethodSUBJECT

CASES:47 diagnosed with asthma-26 males,21 females

CONTROL:47 HEALTHY ADULTS-24 males,23 females

Random sampling from patient’s list that satisfied criteria of the study

Classified asthma severity

Categories:a.symptomsb.lung functionc.activity restrictions

Subjects were informed of the researchers’ objectives

Written consent was obtained

Identified according to American Thoracic Society definition

IV. Materials and Method

Anthropometric measurement were taken

aWeight

b height,

cwaist circumference

dhip circumference

ewaist to hip ratio

fBMI

gBody adiposity index

(hip circumference/heightAssessment of dietary intakesSemi-quantitative food frequency

questionnaire (FFQ)Nutritionist IV program was used to estimate dietary intakes

BIOCHEMICAL ANALYSIS

STATISTICAL ANALYSIS

RESULTS

Fasting venous blood sample

All data were presentedAs mean ±SD

Independent sample t test-comparison of absolute intake of fatty acids and levels of inflammatory factors

Kolmogorov-Smirnov test-normal distribution of all variables

ANCOVA-covariates

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V. Results and Discussion

Presented in Table 3, there is a significant difference between the two groups on the serum levels of inflammatory biomarkers.

Since, serum concentrations of TNF-α, hs-CRP, and leptin were significantly higher in the asthmatic group than in the control group, this denotes an increased inflammatory factor, owing to its pro-inflammatory property, in asthmatic patients

Thus, values significantly differ when compared to a healthy participant.

V. Results and Discussion

As seen in Table 5, Adinocpectin level of both asthmatic and control groups presents a positive correlation to Omega 3 – fatty acids and its derivatives mainly EPA, DHA, and ALA. Thus, the high concentration of Omega 3 Fatty acid and its derivatives may inhibit the action of Adinopectin.

Leptin level also showed a positive correlation with EPA and DHA on both asthmatic and controlled groups but only showed positive correlation for Omega 3 – Fatty acid (gm). While, ALA on the asthmatic group had a negative correlation.

Omega 3 Fatty acid also showed a positive correlation between tNF- alpha and hs –CRP of Asthmatic group, hence only making it inconclusive.

V. Results and Discussion

Higher intake of ω-3 fatty acids and lower levels of inflammatory factors in the healthy control group compared to asthmatic group explains the protective role of essential fatty acids in in asthma.

However, no significant correlation between ω-3 and ω-6 intakes and serum levels of inflammatory factors was found in this study.

Therefore, subsequent studies with larger sample size are needed in this regard.

VI. Conclusion