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Comparative & Experimental Approaches in Aging Biology Research

2018 Schedule

A course by:

Aric N. Rogers, Assistant Professor, MDI Biological Laboratory

&

Ron Korstanje, Assistant Professor, The Jackson Laboratory

Partners

THE JACKSON LABORATORY NATHAN SHOCK CENTER

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July 28, 2018 (Sat)

Event Location

5PM – 6PM Student arrival, check-in

Information provided by Judi Alexander in the student packet

Smith Office

6PM – 7PM Dinner Dining Hall

7PM – 9PM Round table discussion with introductions and course overview Maine Center for Biomedical Innovation, second floor

July 29, 2018 Sunday

Before 9AM Self-service breakfast Dining Hall

9AM – 10:30AM A Brief History of Aging Research & The Revolution Sparked by a Microscopic Roundworm

Aric Rogers

Maine Center for Biomedical Innovation, second floor

10:30AM – 10:45AM

Break

10:45AM – Noon CRISPR in C. elegans

Dustin Updike

(At the end of the presentation, students will be led to the Maine Center for Biomedical Innovation, first floor for a brief tour to know where to go after lunch)

Maine Center for Biomedical Innovation, second floor

Noon – 1PM Lunch Dining Hall

1PM – 5PM Microinjection of CRISPR Constructs in C. elegans

Dustin Updike and Catherine Sharp

Maine Center for Biomedical Innovation, first floor

5PM – 6PM Roundtable discussion - refreshments Dining Hall

6PM – 7PM Dinner Dining Hall

7PM – 9PM Microinjection of CRISPR Constructs in C. elegans

Microinjection of CRISPR constructs

Maine Center for Biomedical Innovation, first floor

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July 30, 2018 Monday

Before 8:30AM Self-service breakfast Dining Hall

8:30AM – 10AM Introduction to Mouse Genetics

Ron Korstanje

Maine Center for Biomedical Innovation, second floor

10AM – 10:30AM Break

10:30AM – Noon Aging Studies in Mice

George Sutphin

Maine Center for Biomedical Innovation, second floor

Noon – 1PM Lunch Dining Hall

1PM – 3:45PM R for data analysis

Yuka Takemon

Maine Center for Biomedical Innovation, second floor

4PM - 5PM Autophagy and Aging (official title TBD)

Dana Miller

Maren Auditorium

5PM – 6PM Roundtable discussion and refreshments with Dana Miller Dining Hall

6PM – 7PM Private Dining with Dana Miller Dining Hall

7PM – 9PM Free Time / CRISPR construct Microinjection (optional)

Catherine Sharp

Maine Center for Biomedical Innovation, first floor

July 31, 2018 Tuesday

Before 8:30AM Self-service breakfast Dining Hall

8:30AM – 10AM Dietary Restriction in Mice

Ron Korstanje

Maine Center for Biomedical Innovation, second floor

10AM – 10:30AM Break

10:30AM – Noon Pharmacological Aging Intervention Studies in Mice

George Sutphin

Maine Center for Biomedical Innovation, second floor

Noon – 1PM Lunch Dining Hall

1PM – 3:50PM Workshop: Mouse aging study design

George Sutphin

Maine Center for Biomedical Innovation, second floor

4PM – 5PM Nutrition and Aging (official title TBD)

Dudley Lamming

Maren Auditorium

5PM – 6PM Roundtable discussion and refreshments with Dudley Lamming Dining Hall

6PM – 7PM Private dining with Dudley Lamming Dining Hall

After 7PM Free Time

4

August 1, 2018 Wednesday

Before 8:30AM Self-service breakfast Dining Hall

8:30AM – 10AM Neurological Disease and Aging

Catherine Kaczarowski

Maine Center for Biomedical Innovation, second floor

10AM – 10:30AM Break

10:30AM – Noon Renal and Cardiovascular Disease and Aging

Ron Korstanje

Maine Center for Biomedical Innovation, second floor

Noon – 1PM Lunch Dining Hall

1PM – 3:50PM Survival Analysis

George Sutphin

Maine Center for Biomedical Innovation, second floor

4PM – 5PM Neurodegeneration and Aging (official title TBD)

Duygu Ucar

Maren Auditorium

5PM – 6PM Roundtable discussion and refreshments with Duygu Ucar Dining Hall

6PM – 7PM Private dining Duygu Ucar Dining Hall

After 7PM Free Time

August 2, 2018 Thursday

Before 8:30AM Self-service breakfast Dining Hall

8:30AM – 10AM Aging and Immune Function

Candice Smith

Maine Center for Biomedical Innovation, second floor

10AM – 10:30AM Break

10:30AM – Noon Bone and Aging

Cheryl Ackert-Bicknell

Maine Center for Biomedical Innovation, second floor

Noon – 1PM Lunch Dining Hall

1PM – 3:45PM Genetic Mapping and Aging

Dan Gatti

Maine Center for Biomedical Innovation, second floor

4PM – 5PM Unique Aging Insights from the Nake Mole-Rat (official title TBD)

Rochelle (Shelley) Buffenstein

Maren Auditorium

5PM - 6PM Roundtable discussion and refreshments with Shelley Buffenstein Dining Hall

6PM – 7PM Private dining with Shelley Buffenstein Dining Hall

After 7PM Free Time

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August 3, 2018 Friday

Before 9AM Self-service breakfast Dining Hall

9AM – 10AM Targeting mRNA Translation in Aging Research

Aric Rogers

Maine Center for Biomedical Innovation, second floor

10AM – 10:15AM Break

10:15AM – Noon Targeting mRNA Translation in Aging Research

Polysome profiling in C. elegans

Aric Rogers and Jarod Rollins

Maine Center for Biomedical Innovation, first floor

Noon – 1PM Lunch Dining Hall

1PM – 3:45PM Targeting mRNA Translation in Aging Research

Polysome profiling in C. elegans

Aric Rogers and Jarod Rollins

Maine Center for Biomedical Innovation, first floor

4PM – 5PM The Role of Protein Aggregation in Age-related Disease

Robert Shmookler Reis

Maren Auditorium

5PM – 6PM Roundtable discussion and refreshments with Robert Shmookler Reis

Dining Hall

6PM – 7PM Private Dining with Robert Shmookler Reis Dining Hall

After 7PM Free time

August 4, 2018 Saturday

Before 10AM Free morning / self-service breakfast Dining Hall

10AM – 11AM Addressing Healthspan and Gerospan in Invertebrate Models

Assessing health and stress tolerance in C. elegans

Jarod Rollins

Maine Center for Biomedical Innovation, second floor

11 – Noon Introduction to Cell Nonautonomous Mechanisms of Aging

Brief Introduction and Laboratory Prep

Kris Burkewitz

Maine Center for Biomedical Innovation, first floor

Noon – 1PM Lunch Dining Hall

1PM – 6PM Outing to Acadia National Park

Ron Korstanje

Acadia National Park

6PM – 7PM Dinner Dining Hall

After 7PM Free Time

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August 5, 2018 Sunday

Before 9AM Self-service breakfast Dining Hall

9AM – 10AM C. elegans and Cell Nonautonomous Mechanisms of Aging & Longevity

Introduction

Kris Burkewitz

Maine Center for Biomedical Innovation, second floor

10AM – 10:15AM Break

10:15AM – Noon C. elegans and Cell Nonautonomous Mechanisms of Aging & Longevity

C. elegans anatomy and in vivo quantitative fluorescence microscopy, Part 1

Kris Burkewitz

Maine Center for Biomedical Innovation, first floor

Noon – 1PM Lunch Dining Hall

1PM – 4PM C. elegans and Cell Nonautonomous Mechanisms of Aging & Longevity

Lab: C. elegans anatomy and in vivo quantitative fluorescence microscopy, Part 2

Kris Burkewitz

Maine Center for Biomedical Innovation, first floor

4PM – 6PM Break

6PM – 7:00PM Dinner Dining Hall

7PM – 9PM C. elegans and Cell Nonautonomous Mechanisms of Aging & Longevity

C. elegans genetic mini-screen via dsRNA feeding

Kris Burkewitz

Maine Center for Biomedical Innovation, first floor

August 6, 2018 Monday

Before 9AM Self-service breakfast Dining Hall

9AM – 10AM Introduction to the Turquoise Killifish Model of Aging

Dario Valenzano

Maine Center for Biomedical Innovation, second floor

10AM – 10:15AM Break

10:15AM – Noon The Turquoise Killifish Model of Aging

Microbiota transfer and analysis of age-related changes in composition

Dario Valenzano

Maine Center for Biomedical Innovation, first floor

Noon – 1PM Lunch Dining Hall

1PM – 4PM Break

4PM – 5PM Science Café – Sirtuins and Aging

David Sinclair

Dining Hall

5PM – 6PM Roundtable discussion and refreshments with David Sinclair Maren Auditorium

6PM – 7PM Private dining with David Sinclair Dining Hall

August 7, 2018 Tuesday

Before 9AM Self-service breakfast Dining Hall

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9AM – 10AM C. elegans and Cell Nonautonomous Mechanisms of Aging and Longevity - Part 2

Kris Burkewitz

Maine Center for Biomedical Innovation, second floor

10AM-10:15AM Break

10:15 – Noon C. elegans and Cell Nonautonomous Mechanisms of Aging and Longevity - Part 2 (continued)

Kris Burkewitz

Maine Center for Biomedical Innovation, first floor

Noon – 1PM Lunch Dining Hall

1PM – 3:50PM Using Drosophila to Explore the Systemic Coordination of Metabolic Homeostasis During Aging

Rapid analysis of ‘fat’ metabolism for high-throughput screening - Drosophila Lab, Part 2

Maine Center for Biomedical Innovation, first floor

4PM – 5PM Rapamycin and Aging (official title TBD)

Matt Kaeberlein

Maine Center for Biomedical Innovation, first floor Conference Center

5PM – 6PM Roundtable discussion and refreshments with Matt Kaeberlein Dining Hall

6PM – 7PM Private Dining with Matt Kaeberlein Dining Hall

August 8, 2018 Wednesday

Before 9AM Self-service breakfast Dining Hall

9AM – 10AM Turquoise Killifish Model of Aging - Part 2

Dario Valenzano

Davis Classroom

10AM – Noon Break

Noon – 1PM Lunch Dining Hall

1PM – 3:30PM Turquoise Killifish Model of Aging

Comparison of microbiome changes – short-lived killifish versus longer lived zebrafish

Dario Valenzano

Davis Classroom

3:30PM – 3:45PM Break

4PM – 5PM Neurological Maintenance and Aging (official title TBD)

Coleen Murphy

Maren Auditorium

5PM – 6PM Roundtable discussion and refreshments with Coleen Murphy Dining Hall

6PM – 7PM Private Dining with Coleen Murphy Dining Hall

August 9, 2018 Thursday

Before 9AM Self-service breakfast Dining Hall

9AM – 10AM Using Drosophila to Explore the Systemic Coordination of Metabolic Homeostasis During Aging

Jason Karpac

Maine Center for Biomedical Innovation, second floor

10AM – 10:15AM Break

10:15AM – Noon Using Drosophila to Explore the Systemic Coordination of Metabolic Homeostasis During Aging

Rapid Analysis of ‘fat’ Metabolism for High-Throughput Screening - Drosophila Lab, Part 1

Jason Karpac

Maine Center for Biomedical Innovation, first floor

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Noon – 1PM Lunch Dining Hall

1PM – 3:30PM Using Drosophila to Explore the Systemic Coordination of Metabolic Homeostasis During Aging

Rapid analysis of ‘fat’ metabolism for high-throughput screening - Drosophila Lab, Part 2

Jason Karpac

Maine Center for Biomedical Innovation, first floor

3:30PM – 3:45PM Break

4PM – 5PM Senescence, Senolytics and Aging (official title TBD)

Judith Campisi

Maren Auditorium

5PM – 6PM Roundtable discussion and refreshments with Judith Campisi Dining Hall

6PM – 7PM Private dining with Judith Campisi Dining Hall

7PM – 9PM Using Drosophila to Explore the Systemic Coordination of Metabolic Homeostasis During Aging

Imaging and quantification of ‘fat phenotypes’

Jason Karpac

Maine Center for Biomedical Innovation, first floor

August 10, 2018 Friday

Before 9AM Self-service breakfast Dining Hall

9AM – 10AM Wound-induced Polyploidy: A New Way to Heal

Vicki Losick

Maine Center for Biomedical Innovation, second floor

10AM – 10:15AM Break

10:15AM – Noon Using Drosophila to Explore the Systemic Coordination of Metabolic Homeostasis During Aging

‘Fly Tools’ for studying tissue crosstalk

Jason Karpac

Maine Center for Biomedical Innovation, first floor

Noon – 1PM Lunch Dining Hall

1PM – 3:30PM Analysis and recap of laboratory training

Aric Rogers

Maine Center for Biomedical Innovation, second floor

3:30PM – 3:45PM Break

4PM – 5PM Stem Cells and Aging (official title TBD)

Henry Jasper

Maren Auditorium

5PM – 6PM Roundtable discussion and refreshments with Henry Jasper Dining Hall

6PM onward Dining [Lobster Bake] and partying with Henry Jasper Dining Hall

August 11, 2018 Saturday

Students Depart