Chromium ConnectAutomate your single cell workflow

Generate consistent results Reduce single cell data variability

Maximize lab productivity Go from cells to sequencing-ready libraries

Integrated and validated Cell partitioning, barcoding, and library prep

10xgenomics.com/instruments/chromium-connect

Thermal cycler

On-deck thermal cycler for all incubation steps

Performs reverse transcription, amplification, fragmentation, ligation, and sample index PCR

We just 10x'd your productivity

Chromium Automated ControllerCompact and designed for seamless hands-off automation

Performs single cell partitioning and barcoding

GantryMovable liquid-handling pipetting device with precision positioning

Automates hundreds of pipetting steps for the entire single cell workflow

Barcode scanner2D barcode reader to scan all reagents and consumables

Enables experiment tracking and prevents reagent mix-ups

Deck areaIncludes thermal cycler, magnetic plate, and 10x optimized reagent carrier

Single cell partitioning, barcoding, and library prep—together in one optimized instrumentChromium Connect integrates components customized for the 10x Genomics single cell workflow, automation-specific reagents and consumables, and an easy-to-use touchscreen computer. From intuitive self-guided setup to error-proof reagent loading, Chromium Connect is designed so even new users can start running single cell sequencing experiments quickly.

10x optimized reagent carrier

Custom designed carrier for error-proof reagent loading

Fits pre-aliquoted reagent strip tubes for up to 8 samples

Magnetic plate

Integrated magnetic separator to handle all cleanup steps

Uses magnetic beads for DNA purification and fragment size selection

Prepare consistent single cell gene expression libraries with our automated solution Chromium Connect automates your single cell sequencing workflow, letting you go from cells to sequencing-ready libraries with walk-away convenience. This instrument enables you to generate consistent and reproducible single cell gene expression results across experiments and across users. Chromium Connect can reduce errors from manual pipetting and shorten your hands-on time from more than 8 hours to less than an hour. Maximize your day and run your single cell experiments with confidence using an integrated and validated solution.

Automation-specific single cell reagents and consumables10x reagents for Chromium Connect come in strip tube format for easy loading into specially designed carriers. Each strip tube is color coded and barcoded to ensure optimal placement on the deck. Flexibility for 1–8 samples means you can get final libraries every day, without wasting reagents or waiting for large batches. The single-use chip and gasket are also specially designed for use with the Chromium Automated Controller.

Benefits with automation

Hands-on time

Pipetting steps

Interaction points

Walk-away time

80%

90%

70%

80%

Touchscreen computer & softwareBuilt-in touchscreen interface with internet connectivity

Guides user step-by-step through fast and simple experiment setup

• LIMS compatible for sample, reagent and experiment tracking

• Embedded error-handling features for intuitive troubleshooting

Included on deck

10xgenomics.com/instruments/chromium-connect

Thermal cycler

On-deck thermal cycler for all incubation steps

Performs reverse transcription, amplification, fragmentation, ligation, and sample index PCR

We just 10x'd your productivity

Chromium Automated ControllerCompact and designed for seamless hands-off automation

Performs single cell partitioning and barcoding

GantryMovable liquid-handling pipetting device with precision positioning

Automates hundreds of pipetting steps for the entire single cell workflow

Barcode scanner2D barcode reader to scan all reagents and consumables

Enables experiment tracking and prevents reagent mix-ups

Deck areaIncludes thermal cycler, magnetic plate, and 10x optimized reagent carrier

Single cell partitioning, barcoding, and library prep—together in one optimized instrumentChromium Connect integrates components customized for the 10x Genomics single cell workflow, automation-specific reagents and consumables, and an easy-to-use touchscreen computer. From intuitive self-guided setup to error-proof reagent loading, Chromium Connect is designed so even new users can start running single cell sequencing experiments quickly.

10x optimized reagent carrier

Custom designed carrier for error-proof reagent loading

Fits pre-aliquoted reagent strip tubes for up to 8 samples

Magnetic plate

Integrated magnetic separator to handle all cleanup steps

Uses magnetic beads for DNA purification and fragment size selection

Prepare consistent single cell gene expression libraries with our automated solution Chromium Connect automates your single cell sequencing workflow, letting you go from cells to sequencing-ready libraries with walk-away convenience. This instrument enables you to generate consistent and reproducible single cell gene expression results across experiments and across users. Chromium Connect can reduce errors from manual pipetting and shorten your hands-on time from more than 8 hours to less than an hour. Maximize your day and run your single cell experiments with confidence using an integrated and validated solution.

Automation-specific single cell reagents and consumables10x reagents for Chromium Connect come in strip tube format for easy loading into specially designed carriers. Each strip tube is color coded and barcoded to ensure optimal placement on the deck. Flexibility for 1–8 samples means you can get final libraries every day, without wasting reagents or waiting for large batches. The single-use chip and gasket are also specially designed for use with the Chromium Automated Controller.

Benefits with automation

Hands-on time

Pipetting steps

Interaction points

Walk-away time

80%

90%

70%

80%

Touchscreen computer & softwareBuilt-in touchscreen interface with internet connectivity

Guides user step-by-step through fast and simple experiment setup

• LIMS compatible for sample, reagent and experiment tracking

• Embedded error-handling features for intuitive troubleshooting

Included on deck

10xgenomics.com/instruments/chromium-connect

Chromium Connect EnablesWalk-Away ConvenienceChromium Connect automates the construction of single cell, sequencing-ready libraries from cell suspensions.By increasing your walk-away time to up to 8.5 hours and reducing the number of manual pipetting steps, you can regain your freedom to focus on other essential activities.

PHASE 1Sample and ReagentPreparation

PHASE 2Single Cell Partitioning, Barcoding, and ReverseTranscription

PHASE 3cDNA Amplificationand Cleanup

PHASE 4Library Construction,Cleanup, andSample Indexing

PHASE 5Final Library QCand Quantification

Automated ManualINSTRUMENTSREQUIRED:

GEMCheck

cDNAQC

INSTRUMENTSREQUIRED:

8.5 HourWalk-Away Time

PIPETTING STEPS

CONSUMABLES

OPTIONAL QC

AUTOMATED

MANUAL

Learn more at 10xgenomics.com© 2019 10X Genomics, Inc. FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.SAM000080 Rev A Chromium Connect Workflow

Step 3Quantify library

Step 4Set up qPCR

Step 5Run qPCR

Step 2Run ChromiumConnect

Step 1Prepare cellsand reagents

Step 8Quantify library

Step 4Amplifly cDNA;Clean up

Step 9Set up qPCR

Step 10Run qPCR

Step 7Index sample;Clean up

Step 6Ligate adaptors;Clean up

Step 5Fragment, End-repair,A-tailing; Clean up

Step 3Run GEM-RT;Clean up

Step 2Run ChromiumController

Step 1Prepare cellsand reagents

15 1015 10

15 5

0 8

10 3

1 2

20 6

15 6

10 5

10 5

15 5

30+ 8

Go from cell suspensions to sequencing-ready libraries with walk-away convenienceMake the most out of every workday with the benefits of automation. Manual methods to prepare single cell sequencing libraries require over 8 hours and hundreds of pipetting steps. Chromium Connect combines single cell partitioning, barcoding, and library preparation in a standardized, automated workflow.

This minimizes pipetting steps and decreases hands-on time to less than 1 hour. Forget about sample-to-sample and user-to-user variability, or even site-to-site handling differences. Let automation do the work and maximize your lab productivity, your walk-away time, and your resources.

© 2019 10X Genomics, Inc. FOR RESEARCH USE ONLY AND NOT FOR USE IN DIAGNOSTIC PROCEDURES. LIT000064 Rev A Chromium Connect Product Brochure

Learn more at 10xgenomics.com

Chromium Connect service and supportOur automation experts are dedicated to helping you get the most out of your 10x Genomics instrument by offering on-site installation, preventive maintenance, and remote support.

InstallationWith this service, our experts will install and verify the instrument for proper performance.

MaintenanceTrained field service engineers will conduct regular preventative maintenance tests.

SupportTechnical support staff are ready to troubleshoot and respond to any instrument issues.

HEPA CAP filtration optionFor labs that need to process samples in a clean, positive pressure environment, HEPA Clean Air Protection (CAP) filtration system is available for Chromium Connect. The integrated hood system saves space and monitors airflow, temperature, and humidity.

Chromium Automated Controller for partitioning

and barcoding

Magnetic platefor bead purification

Thermal cycler for amplification and incubation

Inside Chromium Connect

Sequencing-readylibraries

Data analysisData visualization

Single celllibrary constructionSample prep Sequencing

Enabling seamless lab operationsChromium Connect works as part of an end-to-end single cell sequencing solution—from sample preparation support to turnkey data analysis and visualization tools.