CESI 8000 High Performance Separation System - SCIEX · CESI 8000 High Performance Separation...

Preinstallation Manual

CESI 8000

High Performance Separation

System

Preinstallation

September 2015

Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821 U.S.A.

B11953AC

Preinstallation

CESI 8000 High Performance Separation System

PN B11953AC (September 2015)

© 2015 Beckman Coulter, Inc.

All rights reserved.

Beckman Coulter, Inc. grants a limited non-exclusive

license to the owner or operator of a CESI 8000 instrument

to make a copy, solely for laboratory use, of any portion or

all of the online help and electronic documents shipped

with the CESI 8000 instrument.

Beckman Coulter, the stylized logo and OptiMS are

trademarks of Beckman Coulter, Inc., and are registered in

the USPTO.

All other trademarks are the property of their respective

owners.

Find us on the World Wide Web at:

www.beckmancoulter.com

贝克曼库尔特有限公司，

美国加利福尼亚州，Brea 市，S. Kraemer 大街 250 号，

邮编：92821 电话：(001) 714-993-5321

Printed in U.S.A.

For research use only. Not for use in diagnostic procedures.

http://www.beckmancoulter.com

SCIEX Separations

CE and LC taken to the next level - consolidating expertise for more

powerful separations

Liquid chromatography (LC) and capillary electrophoresis (CE) are among the most powerful tools available to scientists today. AB SCIEX is expanding the power of these technologies by combining the nano and micro liquid chromatography expertise of our Eksigent solutions and the microscale separation expertise from Beckman Coulter Life Sciences, to create the business unit known as SCIEX Separations.

The goal, to create a new generation of separation systems and techniques that incorporate nano LC, micro LC, analytical flow LC, CE, and MS in new and more innovative ways. Our advances in these areas will lead to complementary and orthogonal solutions that will help drive breakthroughs by enabling scientists to address a wider range of analytical challenges.

SCIEX Separations. Be part of it.

For contact details and additional information please visit: http://www.sciex.com/ce

B11953AC iii

http://www.sciex.com/ce

B11953ACiv

Revision History

Revision History

Second Revision, B11953AC, September 2015

Updates were made to:

CHAPTER 2, CESI 8000 Computer System Specifications

First Revision, B11953AB, January 2014

Numerous updates and corrections made.

AB SCIEX consolidation with Beckman Coulter page added for SCIEX Separations.

Initial Issue, B11953AA, December 2013

CESI 8000 Software version 10.1 (includes 32 Karat software)

CESi 8000 Firmware version 10.1

Note: Changes or additions that are part of the most recent revision are indicated in text by a bar in the margin of the amended page.

This document applies to the latest software and firmware listed above, and any higher subsequent versions. When a subsequent software or firmware version affects the information in this document, a new issue will be released to the Beckman Coulter website. For updates, go to www.beckmancoulter.com and download the latest version of the manual.

http://www.beckmancoulter.com

Safety, Notices and Labels

Overview

A description of the symbols and labels that are used on the Beckman Coulter CESI 8000 High Performance Separation System, or that are shown in this manual, can be found in this section.

Do not attempt to perform any procedure before carefully reading all instructions. If in doubt as to how to proceed in any situation, contact your Beckman Coulter Representative.

Beckman Coulter, Inc. urges its customers and employees to comply with all national health and safety standards such as the use of barrier protection. This may include, but is not limited to, protective eyewear, gloves, and suitable laboratory attire when operating or maintaining this or any other automated laboratory instrumentation.

WARNING

If the equipment is used in a manner not specified by Beckman Coulter, Inc., the

protection provided by the equipment may be impaired.

Alerts for Warning, Caution, Important, and Note

All Warnings and Cautions in this document include an exclamation point, framed within a triangle.

The exclamation point symbol is an international symbol which serves as a reminder that all safety instructions should be read and understood before installation, use, maintenance, and servicing are attempted.

WARNING

WARNING indicates a potentially hazardous situation which, if not avoided, could

result in death or serious injury. It may also be used to indicate the possibility of

erroneous data that could result in an incorrect diagnosis. May be used to indicate

the possibility of severe instrument damage.

CAUTION

CAUTION indicates a potentially hazardous situation, which, if not avoided, may

result in minor or moderate injury. It may also be used to alert against unsafe

practices. May be used to indicate the possibility of erroneous data that could

result in an incorrect diagnosis.

IMPORTANT Used for comments that add value to the step or procedure being performed. Following the

advice in the Important adds benefit to the performance of a piece of equipment or to a process.

NOTE Used to call attention to notable information that should be followed during installation, use, or

servicing of this equipment.

B11953AC v


Instrument Safety Precautions

Instrument Safety Precautions

WARNING

Risk of operator injury if:

• All doors, covers and panels are not closed and secured in place prior to and

during instrument operation.

• The integrity of safety interlocks and sensors is compromised.

• You contact moving parts.

• You mishandle broken parts.

• Doors, covers and panels are not opened, closed, removed and/or replaced

with care.

• Improper tools are used for troubleshooting.

To avoid injury:

• Keep doors, covers and panels closed and secured in place while the

instrument in use.

• Take full advantage of the safety features of the instrument. Do not defeat

safety interlocks and sensors.

• Acknowledge and act upon instrument alarms and error messages.

• Keep away from moving parts.

• Report any broken parts to your Beckman Coulter Representative.

• Use the proper tools when troubleshooting.

CAUTION

System integrity could be compromised and operational failures could occur if:

• This equipment is used in a manner other than specified. Operate the

instrument as instructed in the Product Manuals.

• You introduce software that is not authorized by Beckman Coulter into your

computer. Only operate your system’s computer with software authorized by

Beckman Coulter.

• You install software that is not an original copyrighted version. Only use

software that is an original copyrighted version to prevent virus

contamination.

CAUTION

If you purchased this product from anyone other than Beckman Coulter or an

authorized Beckman Coulter distributor, and, if it is not presently under a

Beckman Coulter Service Maintenance Agreement, Beckman Coulter cannot

guarantee that the product is fitted with the most current mandatory engineering

revisions or that you will receive the most current information bulletins

B11953ACvi


Moving Parts or Sharp Objects

concerning the product. If you purchased this product from a third party and

would like further information concerning this topic, contact your Beckman

Coulter Representative.

Moving Parts or Sharp Objects

WARNING

Risk of personal injury. To avoid injury due to moving parts, observe the following:

• Never attempt to exchange labware, reagents, or tools while the instrument

is operating.

• Never attempt to physically restrict any of the moving components of the

instrument.

• Keep the instrument work area clear to prevent obstruction of the movement.

Electrical Safety

To prevent electrically related injuries and property damage, properly inspect all electrical equipment prior to use and immediately report any electrical deficiencies. Contact a Beckman Coulter Representative for any servicing of equipment requiring the removal of covers or panels.

Safety Symbols and Labels

High Voltage Electric Shock Risk Symbol

This symbol indicates that there is high voltage and there is a risk of electric shock, and the operator should use care when accessing this area.

144557-AB

B11953AC vii


RoHS Notices

Sharp Object Label

This symbol indicates that there are sharp objects, and the operator should use care when accessing this area.

Mobile Cart Caution Label

A label cautioning users when moving the cart or adjusting the height of the cart can be found on the CESI 8000 Mobile Cart.

The CESI 8000 instrument is situated on the mobile cart. The OptiMS cartridge spray tip (also known as the consumable) on the CESI 8000 instrument has to be in very close proximity to a Mass Spec when the system is used. To avoid damage to the OptiMS consumable, remove it from the MS adapter before adjusting the height or moving the cart.

RoHS Notices

These labels and materials declaration table (the Table of Hazardous Substance’s Name and Concentration) are to meet People’s Republic of China Electronic Industry Standard SJ/T11364-2006 “Marking for Control of Pollution Caused by Electronic Information Products” requirements.

China RoHS Caution Label

This label indicates that the electronic information product contains certain toxic or hazardous substances. The center number is the Environmentally Friendly Use Period (EFUP) date, and indicates the number of calendar years the product can be in operation. Upon the expiration of the

B11953ACviii


Other Instrument Labels

EFUP, the product must be immediately recycled. The circling arrows indicate the product is recyclable. The date code on the label or product indicates the date of manufacture.

China RoHS Environmental Label

This label indicates that the electronic information product does not contain any toxic or hazardous substances. The center “e” indicates the product is environmentally safe and does not have an Environmentally Friendly Use Period (EFUP) date. Therefore, it can safely be used indefinitely. The circling arrows indicate the product is recyclable. The date code on the label or product indicates the date of manufacture.


This section provides information for some labels and symbols appearing on the CESI 8000 instrument housing. These labels and symbols may be associated with user-serviceable procedures. Individual hazards associated with a specific procedure in this manual may use these labels and symbols, and are included in Warnings or Cautions within the procedures for that task.

Recycling Label (WEEE)

The symbol of a crossed-out wheeled bin on the product is required in accordance with the Waste Electrical and Electronic Equipment (WEEE) Directive of the European Union.

The presence of this marking on the product indicates:

• That the device was put on the European Market after August 13, 2005 and

• That the device is not to be disposed via the municipal waste collection system of any member state of the European Union.

For products under the requirement of WEEE directive, please contact your dealer or local Beckman Coulter office for the proper decontamination information and details on the take back program

that facilitates proper collection, treatment, recovery, recycling, and safe disposal of this device.

B11953AC ix



CE Mark Label

The CE symbol is a conformity marking indicating that the CESI 8000 instrument meets the requirements of applicable European Conformity directives within the European Economic Area (EEA).

C-Tick Mark Label

The C-Tick symbol on the CESI 8000 instrument is an identification marking indicating that the instrument is compliant with applicable electromagnetic compatibility (EMC) requirements for the Australian and New Zealand markets.

CSA Mark Label

The CSA symbol on the CESI 8000 instrument indicates that the instrument has been certified by a Nationally Recognized Testing laboratory (NRTL) to applicable Laboratory Equipment Safety Standards for the United States and Canada.

B11953ACx

安全、通知和标签

综述

用于 Beckman Coulter CESI 8000 高性能分离系统或在本手册中显示的符号和标签的描述可在本节中找到。

仔细阅读所有说明之前，请勿尝试执行任何操作。在任何情况下如果不知如何处理，请与您的 Beckman Coulter 代表联系。

Beckman Coulter, Inc. 强烈要求其客户和员工遵守所有国家健康和安全标准，如防护的使用。此标准包括但不限于下列事项：操作或维护本仪器或任何其他实验室自动仪器时，请佩戴防护眼镜、手套和合适的实验室装备。

警告

如果设备的使用未能按照 Beckman Coulter 公司所指定的方式进行，该设备所

具备的保护性能可能受损。

警告、注意、重要事项以及注释的提示

本文档中的所有警告与注意都包含感叹号，设在三角形中。

感叹号符号是国际通用符号，用于提示在安装、使用、维护和维修前应阅读并理解所有的安全说明。

警告

“警告”指可能的有害情况，若未加以避免，则会导致死亡或严重伤害。也可用

它来说明有可能出现可能导致错误诊断的错误数据。还可用它来说明仪器严重

损坏的可能性。

注意

“小心”是指如果未能避免可能导致轻微或中度伤害的潜在危险情况。可用于警

示不安全操作。可能用于说明有可能出现可能导致错误诊断的错误数据。

重要用于对正在执行的步骤或程序进行有价值的备注。遵循“重要事项”中的建议有助于改善某

件设备或某个程序的性能。

注释用于提醒在设备安装、使用、或维修过程中应遵照的重要信息。

B11953AC xi


仪器安全防护措施

仪器安全防护措施

警告

如果出现以下情况，操作员会有受到伤害的危险：

• 操作仪器过程中或之前没有关闭所有仪器门、盖板和面板，并确保其安全

到位。

• 安全联锁和传感器的完整性受到损害。

• 接触到活动部件。

• 对破碎的部件处理不当。

• 没有小心地打开、关闭、移去和/或更换仪器门、盖板和面板。

• 使用不正确的工具进行故障排除。

要避免造成伤害，请遵循：

• 在使用仪器时保持仪器门、盖板和面板关闭，并确保其固定到位。

• 充分利用该仪器的安全特性。请勿破坏安全联锁装置和传感器。

• 确认仪器警报和错误消息并进行相应的处理。

• 远离活动部件。

• 向 Beckman Coulter 业务代表报告所有破碎的部件。

• 使用正确的工具来进行故障排除。

注意

以下情况会破坏系统完整性并可能导致操作失败：

• 未按操作要求使用本设备。请按照“产品手册”中说明的方式操作仪器。

• 在计算机中安装了未经 Beckman Coulter 授权的软件。请在系统的计算机上

仅运行 Beckman Coulter 授权的软件。

• 安装的软件并非是具有原始版权的版本。请仅使用具有原始版权的软件，

以防止病毒感染。

注意

如果是从 Beckman Coulter 或 Beckman Coulter 授权分销商之外的另一方购买的

本产品，同时目前也不在 Beckman Coulter 服务维护协议的范围内，则 Beckman

Coulter 不能担保该产品具有最新的强制性工艺修订，也不能担保用户可获得有

关该产品的最新信息公告。如果您从第三方购买了此产品且想要了解这方面的

更多信息，请联系您的 Beckman Coulter 代表。

B11953ACxii


活动部件或尖锐物

活动部件或尖锐物

警告

可能导致人身伤害。为避免遭到活动部件的伤害，请遵守以下操作提示：

• 仪器运转时，请勿调换实验室器具、试剂或工具。

• 不得手动限制仪器上任何组件的运动。

• 保持仪器工作区清空，以防限制组件的活动。

电气安全

为避免电气相关的伤害和财产损失，使用前请正确检查所有的电气设备并及时报告所有的电气缺陷。维修设备时如需拆除盖板或面板，请联系 Beckman Coulter 代表。

安全符号和标签

高压电击危险符号

此符号表示存在高电压和电击危险，并且操作员进入此区域时应小心操作。

尖锐物标签

此符号表示存在尖锐物，并且操作员进入此区域时应小心操作。

144557-AB

B11953AC xiii


RoHS 通知

流动车警告标签

移动车时或调整车的高度时，在 CESI 8000 流动车上会看到用户警告标签。

CESI 8000 仪器位于流动车上。使用系统时，CESI 8000 仪器上的 OptiMS 墨盒喷嘴（也称作消耗品）必须十分接近质谱仪。为了避免损坏 OptiMS 消耗品，在调整高度或移动车之前，请将它从 MS 适配器上移除。

RoHS 通知

这些标签和材料声明表（有害物质的名称和含量表）必须符合中华人民共和国电子行业标准 SJ/T11364-2006 《电子信息产品污染控制标识要求》中的要求。

中国 RoHS 警告标签

该标签表示电子信息产品包含某些有毒或有害物质。中间数字为环保使用期限 (EFUP) 日期，表示产品可运行的年数。EFUP 到期后，必须立即回收产品。环形箭头表示产品可回收。标签或产品上的日期代码为制造日期。

中国 RoHS 环保标签

该标签表示电子信息产品未包含任何有毒或有害物质。中间的 “e” 表示产品具有环保安全性，且无环保使用期限 (EFUP) 日期。因此，可无限期地安全使用。环形箭头表示产品可回收。标签或产品上的日期代码为制造日期。

B11953ACxiv


其他仪器标签

其他仪器标签

本节提供了出现在 CESI 8000 仪器外罩上的一些标签和符号信息。这些标签和符号可能与用户自行执行的操作过程有关。本手册中，与特定操作过程相关的各种风险可能使用这些标签和符号，详见该任务操作过程中的“警告”或“注意”部分。

环保标签 (WEEE)

按照欧盟报废电子电气设备 (WEEE) 指令要求，产品上必须标有带叉的有轮垃圾桶符号。

产品上出现该标志，说明：

• 该设备是在 2005 年 8 月 13 日以后投放欧洲市场的，并且

• 设备将不通过欧盟的任何成员国的市政废物收集系统进行处置。

对于 WEEE 指令规定的产品，请联系您的经销商或当地的 Beckman Coulter 办公室，了解有关合理净化和收回程序的详细信息，这有助于您正确收集、处理、复原、回收和安全处置设备。

CE 标志标签

CE 符号是个符合性标记，说明 CESI 8000 仪器符合欧洲经济区 (EEA) 内适用的欧洲符合性指令要求。

B11953AC xv


其他仪器标签

C-Tick 标志标签

CESI 8000 仪器上的 C-Tick 符号是个识别标志，说明了该仪器符合澳大利亚和新西兰市场上适用的电磁兼容性 (EMC) 要求。

CSA 标识标签

CESI 8000 仪器上的 CSA 符号表明，该仪器被国家认可测试实验室 (NRTL) 认证为符合适用的美国和加拿大实验室设备安全标准。

B11953ACxvi

Contents

SCIEX Separations, iii

CE and LC taken to the next level - consolidating expertise for more powerful separations, iii

SCIEX Separations. Be part of it., iii

Revision History, iv

Safety, Notices and Labels, v

Overview, v

Alerts for Warning, Caution, Important, and Note, v

Instrument Safety Precautions, vi

Moving Parts or Sharp Objects, vii

Electrical Safety, vii

Safety Symbols and Labels, viiHigh Voltage Electric Shock Risk Symbol, viiSharp Object Label, viiiMobile Cart Caution Label, viii

RoHS Notices, viiiChina RoHS Caution Label, viiiChina RoHS Environmental Label, ix

Other Instrument Labels, ixRecycling Label (WEEE), ixCE Mark Label, xC-Tick Mark Label, xCSA Mark Label, x

安全、通知和标签 , xi

综述 , xi

警告、注意、重要事项以及注释的提示 , xi

仪器安全防护措施 , xii

活动部件或尖锐物 , xiii

xvii

Contents

电气安全 , xiii

安全符号和标签 , xiii高压电击危险符号 , xiii尖锐物标签 , xiii流动车警告标签 , xiv

RoHS 通知 , xiv中国 RoHS 警告标签 , xiv中国 RoHS 环保标签 , xiv

其他仪器标签 , xv环保标签 (WEEE), xvCE 标志标签 , xvC-Tick 标志标签 , xviCSA 标识标签 , xvi

Introduction, xxiii

Overview, xxiii

Customer Preinstallation Checklist and Sign-off Sheet, xxiv

CHAPTER 1: Preinstallation Requirements, 1-1

Preparation Check List, 1-1CESI 8000 Requirements , 1-1CESI 8000 System Components, 1-1

System Components , 1-1Technical Support, 1-2

CHAPTER 2: Site Requirements and Specifications, 2-1

Preparing the Site, 2-1

CESI 8000 System Specifications, 2-2CESI 8000 Instrument , 2-2CESI 8000 Mobile Cart, 2-3CESI 8000 Computer System, 2-4Mass Spec Software Requirements, 2-5

Space and Ventilation Requirements, 2-6Space Requirements, 2-6Ventilation Requirements, 2-6

Electrical Requirements, 2-7Additional Electrical Requirements, 2-7

CHAPTER 3: Preparing CESI 8000 for Setup, 3-1

Upon Receiving the CESI 8000 System, 3-1Moving the Boxes, 3-1Confirming the Setup, 3-1Safety Considerations During Setup, 3-2MS Setup, 3-2

xviii

Contents

CHAPTER 4: CESI 8000 System Training, 4-1

Pre-Training Requirements, 4-1

Additional Training, 4-1

Index 索引

Beckman Coulter, Inc.Warranty and Returned Goods Requirements

xix

xx

Illustrations

Illustrations

2.1 CESI 8000 Instrument Dimensions, 2-3

2.2 CESI 8000 Mobile Cart Dimensions, 2-4

2.3 Typical CESI 8000 System Setup, 2-6

3.1 Remove 1-inch Adapter Ring, 3-2

3.2 Ion Sweep Cone (remove if in place), 3-3

Tables

Tables

2.1 CESI 8000 Site Requirements, 2-1

2.2 CESI 8000 Instrument Specifications, 2-2

2.3 CESI 8000 Mobile Cart Specifications, 2-4

2.4 CESI 8000 Computer System Specifications, 2-5

2.5 CESI 8000 Electrical Components and Requirements, 2-7

xxi

Tables

xxii

Introduction

Overview

This manual details the necessary site preparation required before installing the CESI 8000 High Performance Separation System. Pay careful attention to the specifications and requirements described in this manual. Accurate and timely preparation allows the Beckman Coulter Field Service Engineer to install the instrument efficiently upon arrival.

The supplies you must have on hand prior to the installation of the instrument are specified. Also provided is a list of accessories available.

For your convenience, a Preparation Check List is included to help you prepare for the installation.

This preinstallation manual covers:

• Preinstallation Requirements

• Site Requirements and Specifications

• Preparing CESI 8000 for Setup

• CESI 8000 System Training

IMPORTANT The “Customer Preinstallation and Sign-off Sheet” on the next page must be completed and

sent to your CESI 8000 representative before installation of the CESI 8000 system can begin.

B11953AC xxiii

Introduction

Customer Preinstallation Checklist and Sign-off Sheet

Customer Preinstallation Checklist and Sign-off Sheet

The following is a list of lab items that are required at the site before installation of the CESI 8000 system can occur. Use this checklist to verify that you have the lab items necessary to ensure a successful installation.

I have reviewed this manual, and I have all of the lab items listed above (please check mark each item).

I also have a copy of the MS Performance Report proving that the MS is fully operational.

IMPORTANT Please copy or scan this page and send it along with a copy of your MS Performance Report to

your AB SCIEX field service representative. Installation of the CESI 8000 System will not be scheduled

until this page and the MS Performance Report have been received.

Lab Item Required Check Yes or No Comments

1.0 N NaOH

(Sigma P/N 319511 or equivalent)

� Yes

� No

0.1 N HCl

(Sigma P/N 84428 or equivalent)

� Yes

� No

0.1 N NaOH

(Beckman Coulter P/N 338424 or equivalent)

� Yes

� No

Beta Galactosidase

(AB SCIEX P/N 4465938)

� Yes

� No

Glacial Acetic Acid

(Sigma P/N A6283 or equivalent)

� Yes

� No

Methanol

(Fisher P/N A454 or equivalent)

� Yes

� No

DDI Water or MS Grade Water � Yes

� No

7.5 M ammonium acetate

(Sigma P/N A2706)

� Yes

� No

pH Meter � Yes

� No

Benchtop Centrifuge � Yes

� No

Correct Nanosource

• AB SCIEX NanoSpray III

• Thermo Scientific NanoSpray II

� Yes

� No

Correct MS Software (see pg. 2-5)

• For AB SCIEX must be Analyst TF 1.6 Build

6211

� Yes

� No

Signature: _______________________________________ Initial: _______________ Date: __________________

B11953ACxxiv

CHAPTER 1

Preinstallation Requirements

Preparation Check List

The following is a summarized list of the requirements that must be addressed, and the components that must be obtained, prior to installation. To ensure a successful installation, please verify that the requirements have been fulfilled and that you have the system components listed below. When completed, contact your local Beckman Coulter representative to schedule the installation.

IMPORTANT CESI 8000 customers (i.e., up to two operators) must be Mass Spectrometer trained, willing to

be trained, and available to operate the Mass Spectrometer during installation.

CESI 8000 Requirements

Use this list to check the requirements that must be fulfilled at your site prior to installation.

• All reagents must be ordered and present at the site by the time of installation (Preparing the Site in CHAPTER 2, Site Requirements and Specifications)

• Suitable laboratory environment (CHAPTER 2, Site Requirements and Specifications)

• Appropriate space for cart and instrument (Space and Ventilation Requirements in CHAPTER 2, Site Requirements and Specifications)

• Power source (Electrical Requirements in CHAPTER 2, Site Requirements and Specifications)

• All components arrived undamaged (Upon Receiving the CESI 8000 System in CHAPTER 3, Preparing CESI 8000 for Setup)

• Training prerequisites met (CHAPTER 4, CESI 8000 System Training)

CESI 8000 System Components

The following is a list of the primary components shipped by Beckman Coulter for a CESI 8000 system. Use this list to check off the items as you receive them.

System Components

• CESI 8000 Instrument

• CESI 8000 Mobile Cart

• CESI 8000 Computer System

B11953AC 1-1

Preinstallation Requirements

Preparation Check List

Technical Support

If you encounter a problem that is not discussed in this guide, and you need technical support, contact your local dealer, the provider of this product, or contact Beckman Coulter directly by using the information below.

NOTE Whenever you call your local dealer or Beckman Coulter, be sure to have your instrument serial

number and software version number available.

For future reference, record this information here.

Instrument Serial Number:

Instance Number:

CESI 8000 Software Version:

32 Karat Software Version:

Firmware Version:

Dealer Name:

Dealer Phone Number:

Mail Beckman Coulter, Inc.

250 S. Kraemer Blvd.

Brea, CA 92821

Web address: www.beckmancoulter.com

B11953AC1-2

CHAPTER 2

Site Requirements and Specifications

Preparing the Site

Appropriate site preparation includes verifying:

• The site layout provides adequate work space for the instrument refer to CESI 8000 System Specifications and Space and Ventilation Requirements.

• The mobile cart holding the CESI 8000 instrument and computer system is level and stable so that normal operations of the instrument and computer does not cause the cart to shift, jerk, or sway (refer to CESI 8000 Mobile Cart).

• The site has adequate electrical supply and power quality for CESI 8000 operation (refer to Electrical Requirements).

NOTE The power plug for the CESI 8000 instrument serves as the Disconnecting Device and must

remain easily accessible.

Table 2.1 CESI 8000 Site Requirements

Item Requirement

Environment • Indoor Use Only.

• Recommended range is 15°C (59°F) to 30°C (86°F), and humidity range

of 20% to 60% RH, without large temperature fluctuations.

Power Requirements • 400 watts at 8A (100-120 VAC) or at 6.3A (200-240 VAC), 50-60 Hz, single

phase, 130 W (nominal) for each module.

• Neutral to ground <0.5V

Lab Items Required Provided

By Purchaser

• 1.0 N NaOH (Sigma P/N 319511 or equivalent)

• 0.1 N HCl (Sigma P/N 84428 or equivalent)

• 0.1 N NaOH (Beckman Coulter P/N 338424 or equivalent)

• Beta Galactosidase (AB SCIEX P/N 4465938)

• Glacial Acetic Acid (Sigma P/N A6283 or equivalent)

• Methanol (Fisher P/N A454 or equivalent)

• DDI Water or MS Grade Water

• 7.5 M ammonium acetate (Sigma P/N A2706)

• pH Meter

• Benchtop Centrifuge

B11953AC 2-1


CESI 8000 System Specifications


Use the following specifications to prepare the environment where the system is to be located.

CESI 8000 Instrument

Table 2.2 presents system specifications and requirements for the CESI 8000 High Performance Separation System. The CESI 8000 instrument should be placed on top of the CESI 8000 Mobile Cart. The dimensions for the CESI 8000 instrument are also shown in Figure 2.1.

Table 2.2 CESI 8000 Instrument Specifications

Specification Description

Dimensions • Width:

— 63.5 cm (25 in.)

• Depth:

— 72.4 cm (28.5 in.)

• Height:

— 99.1 cm (39 in.) maximum, cover open

— 73.7 cm (29 in.) cover closed

• Weight / Mass

— 85.3 kg (188 lb)

Ventilation Clearances • Front, Rear, Left, and Right Side (facing instrument)

— 10.16 cm (4.0 in.)

Electrical • Power requirement:

— 100–240 VAC, 5.0A, 50/60 Hz, auto-sensing power supply

• Fuse Rating:

— 8.0 A Slow Blow; 1/4 inch (2 ea.); 100-120 VAC

— 6.3 A Time Delay; 20mm (2 ea.); 200-240 VAC

• Power Consumption:

— Supply voltage must not exceed 10% of nominal

• Installation (Overvoltage) Category:

— Category II

External Outputs • TTL (Transistor–transistor logic):

— 2

• Contact Closures:

— 2

Mass Spectrometer

Compatibility

• Nanosources:

— AB SCIEX NanoSpray IIIa

— Thermo Scientific NanoSpray II

• Thermo Source Adapter Kit (BEC PN B07366) includes 2 relay cables:

— B13901 -- for MS models LTQ, LXQ, and LCQ

— B13902 -- for MS models TQS and Q Exactive

B11953AC2-2


CESI 8000 System Specifications 2

Figure 2.1 CESI 8000 Instrument Dimensions

CESI 8000 Mobile Cart

The CESI 8000 Mobile Cart is designed to provide the tabletop space where the CESI 8000 instrument can be placed. The cart will also hold all of the hardware components that are shipped as the CESI 8000 Computer System. Table 2.3 shows the specifications for the CESI 8000 Mobile Cart. The dimensions for the CESI 8000 instrument are also shown in Figure 2.1.

Environmental • Sound Pressure Level:

— Maximum sound pressure: Less than 70 dB

• Maximum heat dissipation into room under steady-state conditions:

— 400 Watts (1024 Btu/hr)

• Pollution degree:

— 2

• Ambient Operating Environment:

— Temperature Range 15-30°C

— Humidity Range 20% to 60% RH

• Altitude Restrictions:

— Up to 2000m (6,562 ft)

a. The cover for the AB SCIEX Nanosource must be updated per retrofit kit AB SCIEX part number 5030793 (reference BCI PN

B44471).

Table 2.2 (Continued) CESI 8000 Instrument Specifications


B11953AC 2-3



Figure 2.2 CESI 8000 Mobile Cart Dimensions

CESI 8000 Computer System

The CESI 8000 Computer System is made up of the following four components:

• IBM Controller (small form factor PC)

• 22-inch Monitor

• Keyboard

• Mouse

Table 2.3 CESI 8000 Mobile Cart Specifications


Dimensions • Width:

— 91.4 cm (36 in.)

• Depth:

— 73.7 cm (29 in.)

• Height:

— Adjustable from 68.6 cm (27 inches) to 111.8 cm (44 inches)

• Weight / Mass

— 69.0 kg (152 lb)

Electrical • Rated voltage:

— 120VAC @ 60 Hz

— Optional 230 VAC @ 50 Hz

Support Strength • Supportable Weight

— 136.0 kg (300 lb)

B11953AC2-4


CESI 8000 System Specifications 2

Beckman Coulter validates the CESI 8000 software (includes 32 Karat software) for use with the Microsoft Windows 7 Professional 32-bit operating system. In addition, the software design incorporates wide-screen monitor capability for increased workspace. To operate the system properly, use a validated PC (controller). Beckman Coulter validates the controllers to meet all system requirements. The specifications for the CESI 8000 Computer System are shown in Table 2.4.

The CESI 8000 Mobile Cart is designed to hold the CESI 8000 Computer System (listed above).

IMPORTANT Users must have Administrator or Power user permission on the local workstation.

Mass Spec Software Requirements

The AB SCIEX 5600 MS requires the following software to properly interact with the CESI 8000:

• Analyst TF 1.6 (Build 6211)

A Thermo Scientific MS requires the following software to properly interact with the CESI 8000:

• Xcalibur

Table 2.4 CESI 8000 Computer System Specifications


Computer Requirements • CPU:

— IBM PC (currently validated on Lenovo PC (validated PC controller

with minimum 3.1 GHz processor))

• Memory:

— 4 GBytes RAM (minimum)

• Hard Drive:

— 300 GBytes (minimum)

• CD/DVD RW

— Required

• 5 USB Ports (minimum)

— 1 each required for license key, mass spec, keyboard, mouse, and

GPIB cable

• Monitor/Resolution/Colors

— 22-inch True Color Monitor with 1680 x 1050 resolution

• Keyboard and Mouse

— 101 key and Microsoft compatible mouse

• Interface

— USB cable (National Instruments)

— 2 Serial Ports

— 2 Ethernet Ports

• Operating System

— Microsoft Windows 7 Professional (32-bit) with Service Pack 1

• Software

— CESI 8000 version 10.1 or above (includes 32 Karat version 10.1 or

above)

B11953AC 2-5


Space and Ventilation Requirements

Space and Ventilation Requirements

The CESI 8000 High Performance Separation System must be installed on a level and stable working surface capable of supporting 226.7 kg to 272.1 kg (500 to 600 lbs) without bowing by more than 6.35 mm (1/8 in.)

Space Requirements

The CESI 8000 Mobile Cart dimensions are shown in Figure 2.2. In addition to space for the cart itself, allow additional space for the CESI 8000 instrument. Figure 2.3 illustrates the recommended system setup.

Figure 2.3 Typical CESI 8000 System Setup

IMPORTANT For the AB SCIEX CE-MS configuration, a 14-ft Ethernet cable is provided. The maximum space

though between the CESI 8000 and an AB SCIEX 5600 controller should not exceed 13 ft.

Ventilation Requirements

Adequate space must be provided around the CESI 8000 instrument for ventilation and access to the communication connectors. Allow at least 4 inches on all sides. Refer to Figure 2.1 for overall CESI 8000 instrument dimensions.

B11953AC2-6


Electrical Requirements 2

Electrical Requirements

CAUTION

The CESI 8000 High Performance Separation System should be connected to an

uninterruptible power source to avoid exposure to an erratic power supply. An

erratic power supply could damage the power supply of the CESI 8000 instrument

or other electrical components. Consult with your local UPS Supplier to determine

the proper model for your system.

NOTE The power plug for the CESI 8000 instrument serves as the Disconnecting Device and must remain

easily accessible.

The power line to the laboratory should connect directly from a main power line transformer at a power source known to be clear of erratic power loads, spikes, and electromagnetic interference. Power lines reserved for the instrument must have adequate reserve capacity. Normal loading should not exceed 50% of nominal capacity to allow for start-up loads and the addition of new instruments.

Additional Electrical Requirements

The CESI 8000 instrument is shipped with a power cord suitable for North America (nominal 120 VAC, 60 Hz) installations. When necessary, a power cord suitable for non-North America installations will be provided by Beckman Coulter. The instrument requires one grounded electrical outlet.

NOTE There is one universal power strip bolted to the CESI 8000 Cart tabletop that will supply power to the

CESI 8000 instrument, the cart, the controller (PC), and the monitor. The cart has one external cord that

is used to provide power to the tabletop universal power strip.

Verify that the installation site provides the proper electrical supply to the CESI 8000 High Performance Separation System and any accessories requiring power. Table 2.5 defines the electrical requirements CESI 8000 system components.

Table 2.5 CESI 8000 Electrical Components and Requirements

Electrical Component Electrical Requirements

CESI 8000 Instrument • 100–240 VAC, 5.0A, 50/60 Hz, auto-sensing power supply

Mobile Cart • 120VAC @ 60 Hz

Optional 230 VAC @ 50 Hz

CESI 8000 Controller (PC)

Power Requirements

• 100–127VAC @ 4 amps max

200–240VAC @ 2 amps max

Monitor • 100–240VAC @ 1.5 amps max

B11953AC 2-7


Electrical Requirements

B11953AC2-8

CHAPTER 3

Preparing CESI 8000 for Setup

Upon Receiving the CESI 8000 System

The CESI 8000 High Performance Separation System is shipped within several boxes. Each box should be moved to the selected installation site. A Beckman Coulter Field Service Engineer will unpack the boxes and set up the system at installation.

CESI 8000 system preparation includes:

• Moving the Boxes

• Confirming the Setup

• Safety Considerations During Setup

Moving the Boxes

When the boxes arrive, please inspect all of the boxes. Then move them to an appropriate area so that they can be unpacked by the Beckman Coulter Field Service Engineer.

IMPORTANT If there is any visible and significant damage to any of the boxes, do not receive the shipment.

Contact your local dealer or your local Beckman Coulter representative for further instruction.

IMPORTANT If the shipment has already been accepted and damage had/has occurred during shipment, a

damage claim needs to be filed within 14 days of arrival.

NOTE Do not open the boxes until a Beckman Coulter Field Service Engineer arrives.

Confirming the Setup

After the boxes have been moved to the installation site, contact the local Beckman Coulter Customer Technical Support Center to schedule an on-site appointment with a Field Service Engineer. When the Field Service Engineer arrives, he or she will unpack the components from the boxes, and set up the CESI 8000 High Performance Separation System in the desired location.

B11953AC 3-1



Safety Considerations During Setup

When the Field Services Representative arrives to unbox and set up your unit, ensure the following precaution is observed:

CAUTION

A minimum of four people is needed to lift the CESI 8000 instrument. Failure to do

so could result in bodily injury and/or damage to the instrument.

MS Setup

If you are using a Thermo Scientific MS, prior to installing the NanoSpray II source ensure that the 1-inch adapter ring for dynamic nano spray is not installed. See Figure 3.1. Follow the manufacturer's instructions on how to remove this part.

Figure 3.1 Remove 1-inch Adapter Ring

Additionally, ensure the Ion Sweep Cone has been removed from the MS. See Figure 3.2.

1. Socket Head Screws

2. Ion Max Adapter

1. 1-Inch (Deep) Adapter Ring

B11953AC3-2


Upon Receiving the CESI 8000 System 3

CAUTION

The Ion Sweep Cone may be hot. Care must be taken when manipulating this part.

Figure 3.2 Ion Sweep Cone (remove if in place)

1. Gas inlet

B11953AC 3-3



B11953AC3-4

CHAPTER 4

CESI 8000 System Training

Pre-Training Requirements

After the system has been installed, a Beckman Coulter Field Engineer will train up to two operators in basic CESI 8000 system operation.

IMPORTANT The CESI 8000 customers (i.e., up to two operators) must be Mass Spectrometer trained and

willing to operate the Mass Spectrometer during installation.

Successful implementation of the installation is dependent upon the training environment. To ensure a focused and optimized training session, follow these guidelines:

1. It is important that the operators are available and uninterrupted throughout the entire training period.

2. No more than two operators can be trained on the system at any given time.

System operation requires a basic knowledge of Windows 7 Professional. Operators must have the following skills:

• Use Add/Remove programs

• Create, open, save, edit, move, and copy files

• Perform disk management operations

Additional Training

Our training program has been designed in phases to allow operators to focus on and master basic operational elements. Start by making use of the CESI 8000 Training Guide (PN B44982) and other CESI 8000 manuals that can be found on our website.

B11953AC 4-1

CESI 8000 System Training

Additional Training

B11953AC4-2

Index 索引

A

Adapter Ring, 3-2安全

通知 , xi安全通知

电气安全 , xiii活动部件 , xiii尖锐物 , xiii警告 , xi小心 , xi仪器安全防护措施 , xii重要事项 , xi注释 , xi

B

Boxes, 3-1

C

CESI 8000Computer System, 2-4, 2-5Electrical Components and Requirements, 2-7

Instrument, 2-2Instrument Specifications, 2-2Mobile Cart, 2-3Requirements, 1-1Site Requirements, 2-1Space Requirements, 2-6System Components, 1-1Technical Support, 1-2Training, 4-1Ventilation Requirements, 2-6

CESI 8000 Requirements, 1-1CESI 8000 Site Requirements, 2-1CESI 8000 System Training, xxiii, 1-1China RoHS Caution Label, viii

China RoHS Environmental Label, ixComputer System Specifications, 2-5Copyright, 1

D

电气安全 , xiii

E

Electrical, viiElectrical Requirements, 2-7

J

Ion Sweep Cone, 3-2, 3-3

M

Mobile Cart Specifications, 2-4

O

Overview, xxiii

P

Preinstallation Checklist, xxiiiPreinstallation Requirements, xxiiiPreparing CESI 8000 for Setup, xxiiiPreparing the Site, 1-1Pre-Training Requirements, 4-1

R

Receiving the CESI 8000 System, 1-1Requirements, 1-1Revision History (Manual), ivRoHS Notice, viiiRoHS 通告 , xiv

Index-1

Index 索引

S

SafetyNotices, v

Safety considerations during setup, 3-2Safety Notice

caution, velectrical safety, viiimportant, vinstrument safety precautions, vimoving parts, viinote, vsharp objects, viiwarning, v

safety noticeelectrical safety, vii

SetupConfirmation, 3-1Safety considerations, 3-2

Site Preparations, 2-1Site Requirements, 1-1Site Requirements and Specifications, xxiiiSpace Requirements, 1-1Specifications, 1-1System Components, 1-1System Specifications, 2-2

T

Technical Support, 1-2TrainingAdditional, 4-1Pre-training requirements, 4-1

Training Prerequisites, 1-1

W

Warranty, Warranty-1Ventilation Requirements, 1-1

Z?

中国 RoHS 环保标签 , xiv中国 RoHS 警告标签 , xiv

Index-2

Beckman Coulter, Inc.Warranty and Returned Goods Requirements

All standard Beckman Coulter, Inc. policies governing returned goods apply to this product. Subject to the exceptions and upon the conditions stated below, the Company warrants that the products sold under this sales agreement shall be free from defects in workmanship and materials for one year after delivery of the products to the original Purchaser by the Company, and if any such product should prove to be defective within such one year period, the Company agrees, at its option, either (1) to correct by repair or at the Company’s election by replacement, any such defective product provided that investigation and factory inspection discloses that such defect developed under normal and proper use, or (2) to refund the purchase price. The exceptions and conditions mentioned above are as follows:

1. Components or accessories manufactured by the Company which by their nature are not intended to and will not function for one year are warranted only to reasonable service for a reasonable time. What constitutes a reasonable time and a reasonable service shall be determined solely by the Company. A complete list of such components and accessories is maintained at the factory.

2. The Company makes no warranty with respect to components or accessories not manufactured by it. In the event of defect in any such component or accessory, the Company will give reasonable assistance to Purchaser in obtaining the manufacturer’s own warranty.

3. Any product claimed to be defective must, if required by the Company, be returned to the factory, properly decontaminated of any chemical, biological, or radioactive hazardous material, transportation charges prepaid, and will be returned to the Purchaser with transportation charges collect unless the product is found to be defective.

4. The Company shall be released from all obligations under all warranties, either expressed or implied, if any product covered hereby is repaired or modified by persons other than its own authorized service personnel, unless such repair by others is made with the written consent of the Company.

5. If the product is a reagent or the like, it is warranted only to conform to the quantity and content and for the period (but not in excess of one year) stated on the label at the time of delivery.

It is expressly agreed that the above warranty shall be in lieu of all warranties of fitness and of the warranty of merchantability, and that the company shall have no liability for special or consequential damages of any kind or from any cause whatsoever arising out of the manufacture, use, sale, handling, repair, maintenance, or replacement of any of the products sold under the sales agreement.

Representatives and warranties made by any person, including dealers and representatives of the Company, which are consistent or in conflict with the terms of this warranty, shall not be binding upon the Company unless reduced in writing and approved by an expressly authorized officer of the Company.

Parts replaced during the warranty period are warranted to the end of the instrument warranty.

B11953AC Warranty-1

Beckman Coulter, Inc. Warranty and Returned Goods Requirements

NOTEPerformance characteristics and specifications are only warranted when Beckman Coulter replacement parts are used.

Except as provided in writing signed by an officer to Beckman Coulter, Inc., this system and any related documentation are provided “as is” without warranty of any kind, expressed or implied, including that the system is “error free.” This information is presented in good faith, but Beckman Coulter does not warrant, guarantee, or make any representations regarding the use or the results of the use of this system and related documentation in terms of correctness, accuracy, reliability, currentness, omissions, or otherwise. The entire risk as to the use, results, and performance of this system and related documentation is assumed by the user.

B11953ACWarranty-2

© 2015 Beckman Coulter, Inc.

All Rights Reserved

www.beckmancoulter.com

Related Documents

CESI 8000 User Manual

PN B11949 • System Overview • Integrating CESI 8000 with

Mass Spectrometers • CESI 8000: Installing OptiMS

Cartridge • Thermo Scientific MS:

Integration with CESI 8000 • AB SCIEX TripleTOF 5600 MS:

Integration with CESI 8000 • Overview of 32 Karat

Enterprise Software • Controller and Instrument

Start Up • Using Direct Control to Load

the Cartridge and Samples • Methods and Sequences • Creating and Editing a

Method • Instrument Setup Window • Programming a Sequence • Sequence Validation • CESI 8000 User Interface

Software • Using an AB SCIEX 5600

TripleTOF MS • Using a Thermo Scientific MS • Shutdown Procedures • Decoupling Instruments • Nominal Network

Configuration • 5600 TripleTOF Manual and

Auto-calibration Methods • Auto Vial Increment Feature • Conductive Liquid Capillary

Conditioning • Using Vials and Hardware

Maintenance • Capillary Cleaning Procedure • Capillary Storage Procedure • CESI 8000 Troubleshooting • Fine Tuning Sprayer Tip

Position for Thermo Scientific MS

• Fine Tuning Sprayer Tip Position for AB SCIEX MS

• Mobile Cart Homing Procedure

CESI 8000 System

Administration

PN B11951 • System Administration

Overview • 32 Karat Software System

Administration Overview • System Administration

Options • Managing 32 Karat Software • Enterprise Options For CESI

8000 • Workstation Options

(optional) • Email Options (optional) • General Options (optional) • Instrument Login • System Administration

Wizards • System Administration

Report Utility • Electronic Signature • Global Options • System Activity Log • Example Administration

Setup

• Worksheet

CESI 8000 Software Validation

Pack

PN B11952 • System Overview • Technical Description • Additional Literature • Support Policies • Customer Feedback

Reporting • Supplier Information • Development Environment

• Supporting Documents

CESI 8000 High Performance Separation System - SCIEX · CESI 8000 High Performance Separation...

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