Cell Free DNA comes to the Clinic
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Transcript of Cell Free DNA comes to the Clinic
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HORIZON DIAGNOSTICS
Cell Free DNA comes to the Clinic
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Joe Whittaker
Research Use Only
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Figure - Squamous cell carcinoma of lung (left half of image) replacing normal healthy lung tissue (right side)
The importance of NSCLC therapies is paramount in the fight against cancer. Lung cancer, in particular, is the leading cause of cancer-related deaths worldwide with over 1.1 million patients and 1.2 million new cases of lung cancer diagnosed every year worldwide (1).
1. Jemal, A, et al., et al. Cancer Statistics. A Cancer Journal for Clinicians. 57, 2007, Vol. 1.
Research Use Only
Non-Small Cell Lung Cancer
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Overall, testing cfDNA has four distinct advantages over conventional biopsies, being:
• Cost-effective approach;• Simplified sample collection procedures;• Reduced impact to the patient and;• Easily analyzed.
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Crucially, the combination of these advantages allows for repeat routine testing of the patient through diagnosis to efficacy of treatment.
Research Use Only
Advantages
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“…large variance in concordance rates...different methods of extraction, detection, run conditions, the size and yield of the DNA fragments”
Reference Materials
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However…Variability Arises
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Positive:Positive Positive:Negative Negative:Positive Negative:Negative
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Tumor:cfDNA Result
Figure – adapted from Goto et al., showing the variability between EGFR detection of tumor DNA and cfDNA
Research Use Only
Detected Results Using Either Tumor or cfDNA