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Cancer pathways

045_12_AH

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Ub Ub

UbUb

JNK1ERKs

CDC14BRCA

TBP

PIAS

GADD45

CDK4Cyclin-D1

PCNA

p53

CDK2

Cyclin-E

RbE2F

PML

Rb

DNA-PK

HDAC

p21

CHK 1,2CDC25A

Sirt

p53

p53

p53

p53p53

Ub Ub

UbUb

P P

P

P

P

MKK4

MEKK1

MKK7

MEKK2

PKC-δ

Caspase3

BAX

MEKs

Raf

BAX

APAF1CaspaseCascade &Apoptosis

Transcription

DNA Damage

Double StrandBreaks

G1 S G2 M

BCL2

PCAF

CSNK2

CSNKδ

p300

HIPK2

P

P

P

P

P

P

UV, IonizingRadiation

Osmotic Shock

Proteasome p53 DegradationHIF Pathway

UbiquitinProteolysis

UV, IonizingRadiation

UV, IonizingRadiation

p53

PCNA

PCNA

MDM2

MDM2

MDM2

ATM/ATR

ATM/ATR

p38

p38

p53 pathway Inhibition of Matrix Metalloproteinases (MMPs) VEGF Family ligands and Receptor interactions

EGF pathwayCellular Apoptosis pathway Akt Signaling pathway

DyLight® is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Antibodies Clonality Applications Host Species reactivity Product code

active Caspase 3 P Flow Cyt, ICC, ICC/IF, IHC-FoFr, IHC-Fr, IHC-P, Rb Hu, Ms, Rt, Dm, InMtj, ab13847

IHC (PFA fixed), WB RMk, Xl, Zfsh

active Caspase 3 M WB Rb Hu ab77973

ADAM10 P WB Rb Hu ab84595

ADAM17 P WB Rb Hu ab77820

AKT1 (phospho S473) P Dot Blot, ELISA, ICC/IF, IHC-Fr, IHC-P, IP, WB Rb Hu, Ms, Rt ab66138

ATM (phospho S1981) P ICC/IF, WB Rb Hu ab79891

ATM [2C1 (1A1)] M IHC-P, IP, WB Ms Hu, Ms, Rt, Mk ab78

B Raf [EP152Y] M Flow Cyt, IHC-P, IP, WB Rb Hu, Ms, Rt ab33899

Bad (phospho S136) P ELISA, IHC-P, WB Rb Hu, Ms ab28824

Bax P IHC-Fr, IHC-P, IP, WB Rb Hu, Ms, Rt ab7977

Bcl2 P ICC/IC, IHC-P, WB Rb Hu, Ms ab18210

Bmi1 P ICC/IF, WB Rb Hu ab63767

Caspase 9 P WB Rb Hu ab90236

CCR2 P Flow Cyt, IHC-P, WB Rb Hu, Ms, Rt ab21667

CDKN2A/p19ARF P ELISA, ICC, ICC/IF, IHC-Fr, IHC-P, WB Rb Ms ab80

c-Fos P IHC-Fr, IHC-Fr, IHC-P, WB Goat Hu, Ms, Rt ab87655

c-Myc (phospho T58) P ELISA, ICC/IF, IHC-P, IP, WB Rb Hu, Ms ab28842

E Cadherin [DECMA-1] P Flow Cyt, ICC/IF, IHC-Fr, Inhibition Assay, IP, WB Rt Hu, Ms, Cow, Dog ab11512

E2F1 P IHC-P, WB Rb Hu ab94888

EGFR P IHC-Fr Rb Ms, Rt ab15669

ErbB 2 P IHC-P, WB Rb Hu ab38851

ERK1 + ERK2 P ICC/IF Rb Hu, Ms, Rt ab94484

Estrogen Receptor alpha [33] M Gel supershift assays, ICC/IF, IHC-P, IP,

Real Time PCR, WB Ms Hu, Ms, Rt, Cow, Dog ab2746

Estrogen Receptor beta P ELISA, ICC/IF, WB Rb Hu ab78946

Fibronectin [IST-9] M ICC/IF, IHC-Fr, IHC-P, RIA, WB Ms Hu, Ms, Rt, Chk, Cow, Dog,

Mk, Pig ab6328

GSK3 beta (phospho S9)

[EPR2286Y] M ICC, IHC-P, WB Rb Hu ab75814

HIF1 alpha P ICC/IF Rb Hu ab103063

HIF2 alpha P ICC/IF, IHC-P, WB Rb Hu, Rt ab20654

Hsp27 (phospho S86) P ICC/IF, WB Rb Hu ab93059

JNK1 + JNK2 P WB Rb Hu ab85139

MDM2 P ICC/IF, WB Rb Hu ab87134

MMP2 M IHC-P Ms Hu ab86607

MMP9 P IHC-P, WB Rb Hu ab73734

mTOR [53E11] M WB Ms Hu, Ms, Rt ab87540

MUC1 P ICC/IF, IHC-P, WB Rb Hu ab84597

MUC1 P ICC/IF, IHC-P, WB Rb Hu ab94653

N Cadherin P Flow Cyt, ICC/IF, IHC-Fr, IHC-P, WB Rb Hu, Ms, Rt, Xl ab18203

NFkB p105 / p50 - ChIP Grade P ChIP, Flow Cyt, IHC-Fr, IHC-P, IF, IP, WB Rb Hu, Ms, Rt ab7971

NFkB p65 P ICC/IF, IHC-FoFr, IHC-P, IP, WB Rb Hu, Ms, Rt, Chk, InMtj ab16502

p21 P ICC/IF, IHC-P, Sandwich ELISA, WB Rb Hu, Rt, Cow ab18209

p53 [PAb 240] M ELISA, Flow Cyt, ICC, ICC/IF,

IHC (Methanol fixed), IHC-Fr, IHC-P, IP, WB Ms Hu, Ms, Rt, Dog ab26

pan Cadherin [mAbcam22744] M ICC/IF, IHC-P, WB Ms Hu, Ms, Rt, AGMk ab22744

pan-AKT (phospho T308) P ELISA, IHC-P, WB Rb Hu, Ms, Rt ab38449

PTEN [Y184] M Flow Cyt, ICC/IF, IP, WB Rb Hu, Ms, Rt ab32199

Ras P WB Rb Hu, Ms, Rt, Cow ab69747

ROCK1 P ICC/IF, WB Rb Hu ab105107

Survivin P ICC/IF, IHC-FoFr, IHC-P, WB Rb Hu, Ms, Rt ab24479

Telomerase reverse transcriptase P ICC/IF, WB Rb Hu ab94523

TIE2 P WB Rb Ms, Rt ab71712

TIMP1 [102D1] M ICC/IF, IHC-Fr, IHC-P, WB Ms Hu ab1827

TIMP2 [3A4] M IHC-P, WB Ms Hu, Ms, Rt, Cow, Gpig, Rb ab109708

TNF alpha P IHC-Fr, IHC-P, WB Rb Hu, Ms, Rt ab66579

VEGF [VG-1] M ELISA, ICC, ICC/IF, IHC-Fr, IHC-P, IP, WB Ms Hu, Ms, Rt, Dog ab1316

VEGF Receptor 1 P Electron Microscopy, ICC/IF, IHC-Fr, IHC-P, WB Rb Hu, Ms, Rt, Pig ab2350

VEGF Receptor 2 P ICC/IF, IHC-FoFr, IHC-P, WB Rb Hu, Ms, Rt ab39256

Proteins Description Product code

APRIL protein Recombinant Murine APRIL, a 21.9 kDa protein, 192 amino acids ab50096

c-Jun protein (His tag) Recombinant full length Human c-Jun (amino acids 1-331) with His tag, expressed in a baculovirus system ab84134

CTGF protein (Active) Recombinant fragment, corresponding to amino acids 253-350 of Human CTGF ab50044

MMP2 protein (Human) Full lenght Human MMP2 purified from human fibroblast cell culture media ab40964

Caspase 8 protein (Active) Recombinant active full length mouse Caspase 8 ab61298

Biochemicals Size Description Product code

Phorbol 12-myristate 13-acetate 1mg, 5mg, 25mg PKC activator ab120297

Z-VAD-FMK 1mg, 5mg Irreversible general caspase inhibitor ab120382

Z-D(OMe)E(OMe)VD(OMe)-FMK 1mg, 5mg Cell permeable caspase-3 inhibitor ab120488

LY 294002 5mg, 25mg PI-3 kinase inhibitor ab120243

PP2 1mg, 10mg, 50mg Src and RIP2 kinase inhibitor ab120308

Rottlerin 10mg,50mg Kinase inhibitor ab120377

Secondary antibodies

And for superb IF and ICC results use our pre-adsorbed secondary antibodiesAvailable conjugated to: Cy3® and Cy5®

DyLight® 488, DyLight® 550, DyLight® 594, DyLight® 650

AMCA, FITC, PE, Texas Red® and TRITC

Key: AGMk - African Green Monkey | Chk - Chicken | Dm - Fruit Fly (Drosophila melanogaster) | Gpig - Guinea Pig | Hu - Human | InMtj - Indian Muntjac | Mk- Monkey | Ms - Mouse | Rb - Rabbit | RMk - Rhesus Monkey | Rt- Rat | Xl - Xenopus laevi | Zfsh - Zebrafish