C146-E227A

Ready-to-Use Analytical ConditionsShimadzu Method Packages deliver conditions for efficient and simultaneous multi-component analysis. They enable the user to quickly and easily implement complex methods without costly and laborious method development by providing sample preparation protocols, LC separation conditions, and MS acquisition parameters.

Ion pair or PFPP column methodA PFPP:Pentafluorophenylpropyl column method was newly adapted to expand your choice in separation conditions as well as target compounds.

Supporting LCMS-8030/8040/8050This method package supports the latest Shimadzu UFMS triple quadrupole mass spectrometer series, LCMS-8030, 8040 and 8050. LCMS-8050 delivers best-in-class sensitivity and the fastest scanning and polarity switching rates.

Complete from Sample Preparation to AnalysisProtocols are included for the preparation of extracts from biological tissue. Saving the user time and money, even laboratories unfamiliar with extraction can follow prescribed steps for LC/MS/MS sample preparation.

Normalization of Multiple Sample ResultsDue to mass differences between biological tissue samples, normalization of results must be performed when multiple samples are analyzed. This method package includes optimized analytical conditions for two internal standards to permit normalization across multiple samples.

LC/MS/MS Method Package for Primary Metabolites Ver. 2For LabSolutions Ver. 5

Overlaid MRM chromatograms for 55 standards mixture by ion pair methodArea ratio comparison between two tissues focused on methylation cycle

0.0 1.0 2.0 3.0 4.0 5.0 6.0 7.0 8.0 9.0 10.0 11.0 12.0 13.0min

0

5000

10000

15000

20000

25000

30000

35000

40000

45000

50000

55000

60000 Erythrose-4-phosphateRibulose-5-phosphatePyruvateCMPNADDihydroxyacetonephosphateUMPGMP

LysineArginineHistidineGlycineSerineAsparagine

AlanineGlutamineThreonineCysteineL-Methionine sulfoneMethionine

TyrosineMES (2-Morpholinoethanesulfonic acid)GlutamateAspartatePhenylalanine

Glucose-6-phosphateRibose-5-phosphateSedoheptulose-7-phosphateFructose-6-phosphateTryptophanGlycerol-3-phosphateGlucose-1-phosphateGlyceraldehyde-3-phosphate

TMPAMPcGMPcAMPCDP6-PhosphogluconateGDPUDP3-Phosphoglycerate/2-PhoshoglycerateNADPFructose-1,6-bis-phosphateNADH

ADPTDPPhosphoenolpyruvateCTPGTPUTPATPTTPNADPH2,3-bis-PhosphoglycerateSuccinyl-CoAAcetyl-CoA

55 STDs Mix.

Methionine

S-Adenosylmethionine

Homocysteine

Cystathionine

Cysteine

Glutathione

Oxidized glutathione

Adenosine

Inosine

Uric acid

5-Glutamylcysteine

1 2

1 2

1 2

1 2

1 2

1 2

1 2

1 2

1 21 2

1 2

1 2

S-Adenosylhomocysteine

LCMS-8050

Index of compounds

Remarks and Precautions

1. For Research Use Only. Not for Use in Diagnostic Procedures.

2. Shimadzu makes no warranty regarding the accuracy of information included in the

database or the usefulness of information obtained from using the database.

3. It is the user’s responsibility to adopt appropriate quality control tests using standard

samples to confirm qualitative and quantitative information obtained with this method

package.

LC/MS/MS Method Package for Primary Metabolites Ver. 2For LabSolutions Ver. 5

© Shimadzu Corporation, 2014

2,3-Bisphosphoglyceric acid3-Phosphoglyceric acid(2-Phosphoglyceric acid)Dihydroxyacetone phosphateFructose 1,6-bisphosphateGlucose 1-phosphateGlucose 6-phosphateGlycerol 3-phosphatePhosphoenolpyruvic acidPyruvic acidFructose 6-phosphateGlyceraldehyde 3-phosphate6-Phosphogluconic acidErythrose 4-phosphateRibose 5-phosphateRibulose 5-phosphateSedoheptulose 7-phosphate

Acetyl coenzyme ASuccinyl coenzyme A

AlanineArginineAsparagineAspartic acidCysteineGlutamic acidGlutamineGlycineHistidineLysineMethioninePhenylalanineSerineThreonineTryptophanTyrosine

* The ion pair method is suitable for metabolites associated with a glycolytic system,

pentose-phosphate pathway and either ADP or ATP.

* The PFPP column method is good for covering amino acids, organic acids and metabolites

related to the methylation cycle and urea cycle.

List of compounds for ion pair method List of compounds for PFPP column method

Adenosine 3',5'-cyclic monophosphateAdenosine diphosphateAdenosine monophosphateAdenosine triphosphateCytidine diphosphateCytidine monophosphateCytidine triphosphateGuanosine 3',5'-cyclic monophosphateGuanosine diphosphateGuanosine monophosphateGuanosine triphosphateThymidine diphosphateThymidine monophosphateThymidine triphosphateUridine diphosphateUridine monophosphateUridine triphosphate

NADNADHNADPNADPH

2-Morpholinoethanesulfonic acidMethionine sulfone

Lactic acidPyruvic acid

2-Ketoglutaric acidAconitic acidCitric acidFumaric acidIsocitric acidMalic acidSuccinic acid

4-HydroxyprolineAlanineArginineAsparagineAspartic acidAsymmetric dimethylarginineCitrullineCystineDimethylglycineGlutamic acidGlutamineGlycineHistidineHomocystineIsoleucineLeucineLysineMethionine sulfoxideOrnitinePhenylalanineProlineSerineSymmetric dimethylarginineThreonineTryptophanTyrosineValineCystathionineCysteineHomocysteineMethionine5-GlutamylcysteineGlutathioneOxidized glutathioneS-AdenosylhomocysteineS-Adenosylmethionine

4-Aminobutyric acidAdenylsuccinic acidArgininosuccinic acidCholic acidCreatineNicotinic acidOphthalmic acidOrotic acidPantothenic acidTaurocholic acidUric acid

2-Aminobutyric acidAcetylcarnitineAcetylcholineAllantoinCarnitineCarnosineCholineCiticolineCreatinineCysteamineDopaDopamineEpinephrineHistamineHypoxanthineKynurenineNiacinamideNorepinephrineSerotonin

AdenineCytosineGuanineThymineUracilXanthineAdenosineCytidineGuanosineInosineThymidineUridineAdenosine 3',5'-cyclic monophosphateAdenosine monophosphateCytidine 3',5'-cyclic monophosphateCytidine monophosphateGuanosine 3',5'-cyclic monophosphateGuanosine monophosphateThymidine monophosphate

FADFMNNAD

2-Morpholinoethanesulfonic acidMethionine sulfone

Glyco

lytic system

Nu

cleotid

es

Org

anic acid

sN

ucleo

sides an

d N

ucleo

tides

Oth

ers

Co

-enzym

e

Am

ino

acids

Pento

se-ph

osp

hate

path

way

Inter-

nal

STDs

Glyco-

lyticsystem

Meth

ylation

and

Tran

ssulfu

ration

cycle

TCA

cycle

Am

ino

acids

TCA

cycle

Co

-en

zymes

Inter-

nal

STDs