BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7...

21
20140710_Company_Presentation BUCHI For internal use only page 1 10.07.2014 Loadability BÜCHI Labortechnik AG

Transcript of BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7...

Page 1: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 110.07.2014

Loadability

BÜCHI Labortechnik AG

Page 2: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 210.07.2014

2

Load ability

� Loading Capacity

� Resolution

� Sample Solubility (injection)

� Conclusion

Page 3: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 310.07.2014

3

Vancomycin Frontal Loading

ColumnLoading Capacity

Competitor D, C18 10 µm 31 mg/mL

Competitor C, C18 15µm 11 mg/mL

Competitor B, C18 10µm 7 mg/mL

Competitor A, C18 15-20µm 13 mg/mL0 2 4 6 8 10 12 14 16 18 20 22 24 26 28 30 32 34 36 38 40 42 45

4321

Competitor CCompetitor B

Competitor A

BreakthroughPoint

Time (min.)

Abs

orba

nce

at 2

76 n

m

Competitor D

The capacity of each material is calculated from the following equation:

CCapacity = ([T (at 50%) – t0(uracil) ] x C vancomycin x F)

VColumnT (at 50%) = Frontal breakthrough time measured at 50% peak height minus 1 minute.

Cvancomycin = 1 mg/mL

VColumn = 0.173 mL

F (flow rate) = 0.2 mL/min

t0uracil determined from an injection of uracil with mobile phase A at 0.2mL/min.

Loading Capacity (active sites/sample affinity)

Page 4: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 410.07.2014

4

Loading capacity (Surface Area)

Effect on chromatography

High surface area generally provides greater retention, capacity and resolution for separating complex, multi-component samples.

Low surface area packings generally equilibrate quickly, especially important in gradient analyses.

Page 5: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 510.07.2014

5

0.0 2.5 5.0 7.5 10.0 12.5 15.0 17.5 20.0 22.5 25.0 27.5 30.0

25 µg50 µg100 µg250 µg1000 µg

Time (min.)

Vydac ® 150HC C18 20µm

Sample Load (µg) Vydac® 150HC C18 20µm

Efficiency (plates)

25 2132

50 2149

100 1578

250 1118

1000 365

Loading Capacity (active sites saturation)

Page 6: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 610.07.2014

6

2-fold increase in column inner diameter

4 to 5-fold increase in capacity

C4, 300Å, 4.6 x 250 mm, 5 µm24-95% ACN in 0.1% TFA over 30 min.

Loading capacity (Scaling Up)

Page 7: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 710.07.2014

7

Loading capacity (Particle Sizes)

� Peak widths much narrower with small particle materials at low sample loads.

� However, little difference in peak widths at high loads.

� Allows usage of larger particle sizes resulting in lower cost.

C424-95% ACN in 0.1% TFA over 30 min. at 1.5 mL/min.

Page 8: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 810.07.2014

8

ResolutionRelation between resolution and loadability

When ΔRf increases the sample loadabilityincreases

Page 9: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 910.07.2014

Resolution (choices)

• Column packing material

• pH of eluens

• Temperature

• Selectivity triangle

9

H-

Acceptor

DipoleH-

Donor

TolueneVII

VDichloro-methane

I Diethyl-ether

II

Ethanol

IVAceticacid

III Tetra-

hydrofuranVIII

ChloroformVI

Ethylacetate

Page 10: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1010.07.2014

10

Injection(Analytical)

Page 11: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1110.07.2014

11

Injection(Overload)

Page 12: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1210.07.2014

12

Injection(Overload types)

Page 13: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1310.07.2014

13

Injection(test)

In this part of the comparison 2 types of tests are performed :Loading capacity by volume of injectionLoading capacity by mass of sample

A standard Reveleris® system was used with the following conditions :Solvent : Gradient heptane / ethyl acetate 0 to 25 % in 15 CV.Flow : 36ml/min for all other cartridgesDetection : ELSD + UV1 (254nm) + UV2 (280nm)Sample: Phtalate test mix in Heptane

toluene (5g) dimethylphtalate (10g)diethylphtalate (15g)dibutylphtalate (20g)

Page 14: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1410.07.2014

14

Loading capacity by injection volume

Mass of sample Injection Volume

1.4g 2ml

1.4g 4ml

1.4g 8ml

0

0,2

0,4

0,6

0,8

1

1,2

1,4

1,6

2 4 6

Res

olu

tio

n

volume Injection (ml)

Resolution as function from injection volume

Peak 2-3 Peak 3-4

Page 15: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1510.07.2014

15

Loading capacity by injection volume (solvent strength)

Page 16: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1610.07.2014

16

0

0,2

0,4

0,6

0,8

1

1,2

1,4

1,6

1,8

2

0,7 1,4 2,1 2,8

Res

olu

tio

n

Sample load (gram)

Resolution as function from sample mass

Peak 2-3 Peak 3-4

Loading capacity by sample mass

Page 17: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1710.07.2014

Solutions to overcome injection limitation

o Direct injection on column.

o The injection valves are bypassed.

o Sandwich injection method

o Solid loading

17

Page 18: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1810.07.2014

18

Sandwich Injection (precipitation)

Page 19: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 1910.07.2014

19

Solid loading

Page 20: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 2010.07.2014

20

Solid loading (example)

500mg mix of a mono saccharide and glycosylated peptides.Coated on 3 gram celite

Page 21: BÜCHI Labortechnik AG Loadability...Competitor C, C18 15 µm 11 mg/mL Competitor B, C18 10 µm 7 mg/mL Competitor A, C18 15-20 µm 13 mg/mL 0 2 4 6 8 10 12 14 16 18 20 22 24 26 28

20140710_Company_PresentationBUCHI

For internal use onlypage 2110.07.2014

21

Conclusion

• Load abilities limitation are physical• Solubility• Affinity• Selectivity• Resolution• Precipitation

No controlable : It is impossible to overcome Physical limitation

controlable: There are a lot of parameters to play with-Solvents (pH, selectivity triangle)-packing material (selectivity, service area, particle, active sites etc.)-injection method


InertSustain C18

InertSustain C18

volume ~ 1 µm x 1 µm x 1 µm

volume ~ 1 µm x 1 µm x 1 µm

Competitor Analysis · 2018-10-10 · Competitor F Competitor H Competitor V Kaydon 94.9 97.6 97.8 98.3 Kaydon Competitor V Competitor H Bowser Competitor F Competitor R 400 300 200

Competitor Analysis · 2018-10-10 · Competitor F Competitor H Competitor V Kaydon 94.9 97.6 97.8 98.3 Kaydon Competitor V Competitor H Bowser Competitor F Competitor R 400 300 200

Finisterre™ SPE Columns - · PDF filequantifi cation, and HPLC column life. ... Frit: Polyethylene 20 µm porosity Applications For compounds retained too strongly on C18 or C8

Finisterre™ SPE Columns - · PDF filequantifi cation, and HPLC column life. ... Frit: Polyethylene 20 µm porosity Applications For compounds retained too strongly on C18 or C8

Fluke 971Temperature Humidity Meter - AresAgante, Lda. · 2015-01-12 · Specifications Six size channels 0.3 µm, 0.5 µm, 1.0 µm, 2.0 µm, 5.0 µm, 10.0 µm Flow rate 2.83 L/min

Fluke 971Temperature Humidity Meter - AresAgante, Lda. · 2015-01-12 · Specifications Six size channels 0.3 µm, 0.5 µm, 1.0 µm, 2.0 µm, 5.0 µm, 10.0 µm Flow rate 2.83 L/min

GDD - Production guide v41 · 2020. 6. 4. · Shielding Aluminium (10 µm) GDD GEM foils (50 µm) 50 µm thick kapton, 5 µm copper, 70 µm hole diam., 140 µm pitch EST Drift 2 µm

GDD - Production guide v41 · 2020. 6. 4. · Shielding Aluminium (10 µm) GDD GEM foils (50 µm) 50 µm thick kapton, 5 µm copper, 70 µm hole diam., 140 µm pitch EST Drift 2 µm

Extended length capillary columns for simple peptide ... · Simple Peptide Mapping Column: 400 or 100 mm in Length, 150 µm ID Stationary Phase: Vydac C18, 300A, 5µm Temperature:

Extended length capillary columns for simple peptide ... · Simple Peptide Mapping Column: 400 or 100 mm in Length, 150 µm ID Stationary Phase: Vydac C18, 300A, 5µm Temperature:

METHOD CONVERSION GUIDEBOOK€¦ · HALO® C18, 2.7 µm 339 bar, 7400 plates Superior efficiency with > 2.8x Plates! Sharper peaks Faster analysis High performance with 1/2 the back

METHOD CONVERSION GUIDEBOOK€¦ · HALO® C18, 2.7 µm 339 bar, 7400 plates Superior efficiency with > 2.8x Plates! Sharper peaks Faster analysis High performance with 1/2 the back

ISO 16890 Get the full picture on filter performance....1 µm and PM10 2 µm 3 µm 4 µm 5 µm 6 µm 7 µm 8 µm 9 µm 10 µm 0 µm 1 µm So you get the full picture of how a filter

ISO 16890 Get the full picture on filter performance....1 µm and PM10 2 µm 3 µm 4 µm 5 µm 6 µm 7 µm 8 µm 9 µm 10 µm 0 µm 1 µm So you get the full picture of how a filter

DESIGN FEATURES AND PERFORMANCE OF A DIRECT ......200 fmol enolase digest, analytical column: 1.7 µm BEH C18 75 µm x 100 mm, flow rate: 250 nL/min, gradient: 3-60% B in 30 min 10.00

DESIGN FEATURES AND PERFORMANCE OF A DIRECT ......200 fmol enolase digest, analytical column: 1.7 µm BEH C18 75 µm x 100 mm, flow rate: 250 nL/min, gradient: 3-60% B in 30 min 10.00

Application Note - downloads.leica-microsystems.com EM... · Diamond foils: 15 µm, 9 µm, 6 µm, 3 µm, 1 µm and 0.5 µm at 2200 rpm until the final sample thickness of 30 µm.

Application Note - downloads.leica-microsystems.com EM... · Diamond foils: 15 µm, 9 µm, 6 µm, 3 µm, 1 µm and 0.5 µm at 2200 rpm until the final sample thickness of 30 µm.

Acclaim AmG C18 Columns€¦ · Power function = 1.00) Sample Gentamicin (1 mg/mL) Exposure 100 mM TFA for 50 hours Column Acclaim AmG C18, 3 µm Dimensions 3.0 × 150 mm Mobile phase

Acclaim AmG C18 Columns€¦ · Power function = 1.00) Sample Gentamicin (1 mg/mL) Exposure 100 mM TFA for 50 hours Column Acclaim AmG C18, 3 µm Dimensions 3.0 × 150 mm Mobile phase

Q`QhS:F · c17-18 $330 c17-14 $250 c17-16 $380 c17-15 c17-17 $170 $470 c17-19 $500 c17-20 $340 c17-21 $1010. c18-1 $200 c18-2 $130 c18-3 $100 c18-6 $200 c18-7 $130 c18-8 c18-9 $200

Q`QhS:F · c17-18 $330 c17-14 $250 c17-16 $380 c17-15 c17-17 $170 $470 c17-19 $500 c17-20 $340 c17-21 $1010. c18-1 $200 c18-2 $130 c18-3 $100 c18-6 $200 c18-7 $130 c18-8 c18-9 $200

Trigger-Responsive Chain-Shattering Polymersan analytical C18 column (Luna C18, 250 mm × 4.6 mm, 5 µm, Phenomenex, Torrance, CA, USA). The UV wavelength for detecting pyrene-based

Trigger-Responsive Chain-Shattering Polymersan analytical C18 column (Luna C18, 250 mm × 4.6 mm, 5 µm, Phenomenex, Torrance, CA, USA). The UV wavelength for detecting pyrene-based

Manufacture and Characterization of Graphene Membranes ... · 5 µm × 5 µm × 16.4 µm to 100 µm × 100 µm × 16.4 µm in size.Our approach is compatible with wafer-scale MEMS

Manufacture and Characterization of Graphene Membranes ... · 5 µm × 5 µm × 16.4 µm to 100 µm × 100 µm × 16.4 µm in size.Our approach is compatible with wafer-scale MEMS

IMPROVE YOUR FORMULATIONS ASSAYS TODAY · 2016. 12. 16. · Synergi™ Hydro-RP Synergi Fusion-RP Gemini® C18 Gemini NX-C18 Kinetex® C18 Kinetex EVO C18 Kinetex XB-C18 3 to 10 Silica

IMPROVE YOUR FORMULATIONS ASSAYS TODAY · 2016. 12. 16. · Synergi™ Hydro-RP Synergi Fusion-RP Gemini® C18 Gemini NX-C18 Kinetex® C18 Kinetex EVO C18 Kinetex XB-C18 3 to 10 Silica

Characterization c18

Characterization c18

Dr. Christian E. BUTZKE Enology Professor PURDUE UNIVERSITY · 10 µm10 µm 1 µm1 µm 100 nm100 nm 10 nm10 nm.45 µm.45 µm .2 µm.2 µm Particle Size

Dr. Christian E. BUTZKE Enology Professor PURDUE UNIVERSITY · 10 µm10 µm 1 µm1 µm 100 nm100 nm 10 nm10 nm.45 µm.45 µm .2 µm.2 µm Particle Size

TN-1076 APPLICATIONS TN-1076 ... Mobile Phase: A: 0.1 % Trifluoroacetic acid in Water B: ... netex 2.6 µm C18 column and 3B uses a HALO 2.7 µm C18 column.

TN-1076 APPLICATIONS TN-1076 ... Mobile Phase: A: 0.1 % Trifluoroacetic acid in Water B: ... netex 2.6 µm C18 column and 3B uses a HALO 2.7 µm C18 column.

and 5 µm particles! Restek‘s Speed Up and Simplify GC ... · The established choice for bioanalytical testing since 2005 •eparates compounds that other phenyl and C18 chemistries

and 5 µm particles! Restek‘s Speed Up and Simplify GC ... · The established choice for bioanalytical testing since 2005 •eparates compounds that other phenyl and C18 chemistries