Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and...
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![Page 1: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/1.jpg)
Making Effective Use
of Simple Genomic Tools
in the Beef Herd
Bob Weaber, Ph.D.Cow-Calf Extension Specialist
Associate ProfessorDept. of Animal Sciences and Industry
![Page 2: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/2.jpg)
Overview
Basics of DNA Parentage Tools Panel types Principle of exclusion Pedigree validation vs. construction
Once I have parentage then what? Seedstock Commercial
![Page 3: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/3.jpg)
DNA Parentage Tools
Based on Mendelian inheritance Exclusion based
Exploits violations in Mendelian inheritance pattern
Dependent on variation in population Allows development of probabilistic
statements of parentage Useful for pedigree validation or
construction
![Page 4: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/4.jpg)
DNA Parentage Tools
SNP or Microsatellite Panels SNP- ~100 biallelic markers-Standardized
Panel Microsatellite - ~12 Polymorphic dinucleotide
tandem repeats (Older and phasing out) Need all animals you plan to use for
inference tested on same panel If using stored genotypes at Breed
Association make sure you use the same vendor
![Page 5: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/5.jpg)
Tools for Improving Beef Production Systems--2012 MW-ASAS Meeting--Des Moines, IA 5
Validation vs. Testing
Validation Given progeny, do sire and dam qualify
as parents (non-exclusion) Pedigree integrity checks by breed
associations of significant animals
PP
03/20/2012
![Page 6: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/6.jpg)
Tools for Improving Beef Production Systems--2012 MW-ASAS Meeting--Des Moines, IA 6
Validation vs. Testing
Testing Assignment of paternity (or maternity) One or both alleged parents unknown
03/20/2012
![Page 7: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/7.jpg)
Principle of Exclusion
Possible Sires:
Bull A: AA BB
Bull C: aa bb
Bull B: Aa bb
Progeny Genotype: AA bb
?? ?? ??
![Page 8: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/8.jpg)
Principle of Exclusion
Possible Sires:
Bull A: AA BB
Bull C: aa bb
Bull B: Aa bb
Progeny Genotype: AA bb
![Page 9: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/9.jpg)
Principle of Exclusion
Possible Sires:
Bull A: AA BB
Bull C: aa bb
Bull B: Aa bb
Progeny Genotype: AA bb
X
![Page 10: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/10.jpg)
Principle of Exclusion
Possible Sires:
Bull A: AA BB
Bull B: Aa bb
Progeny Genotype: AA bb
![Page 11: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/11.jpg)
Principle of Exclusion
Possible Sires:
Bull A: AA BB
Bull B: Aa bb
Progeny Genotype: AA bb
X
![Page 12: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/12.jpg)
Principle of Exclusion
Possible Sires:
Bull B: Aa bb
Progeny Genotype: AA bb
![Page 13: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/13.jpg)
Tools for Improving Beef Production Systems--2012 MW-ASAS Meeting--Des Moines, IA 13
Paternity Assignment via DNA Markers
Breeding Pasture with 10 BullsExclusion Probability = 0.97
0%
10%
20%
30%
40%
50%
60%
70%
80%
0 1 2 3 4 5 6 7 8 9 10
# of Extra Bulls Included (in addition to the true sire)
Perc
enta
ge o
f Cal
ves
03/20/2012
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![Page 16: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/16.jpg)
![Page 17: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/17.jpg)
Once I have pedigree
then what?
![Page 18: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/18.jpg)
Commercial Herd
Establish genetic value of calves Computed weighted average pedigree
estimate of group(s)—sort off high merit calves?
Replacement females Selection of AI sired females Selection of half sib groups▪ Simplify future mating decisions
Defect management-calves of only non-carrier bulls
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Commercial Herd
Progeny test of young sires Large integrated ranch that produces
own bulls Strategically mate yearlings to known
age group of cows Establish paternity Genetic evaluation for ERT based on
inferred pedigree and phenotypes collected
![Page 20: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/20.jpg)
Commercial Herd
Outlier management Strategic culling of sires the produce
problem calves▪ Dystocia
▪ Docility
▪ Feet/leg issues
▪ Defects?
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Seedstock
Enable use of multi-sire breeding pastures Bad bull risk management Better pasture utilization Assured pedigree
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Enabling Strategies
Make DNA sample collection part of SOP Collect tissue samples from:▪ Natural service sires
▪ Custom collected AI sires
▪ Donor dams
![Page 23: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/23.jpg)
99th K-State Cattlemen's Day 23
A genomic pedigree
03/02/2012
SNP markers allow identification of regions of chromosome and tracking of inheritance of specific
region
![Page 24: Bob Weaber, Ph.D. Cow-Calf Extension Specialist Associate Professor Dept. of Animal Sciences and Industry bweaber@k-state.edu.](https://reader036.fdocuments.us/reader036/viewer/2022062516/56649db65503460f94aa8876/html5/thumbnails/24.jpg)
Thank you!Questions?