Biotechnology. Comparative genomics also has been used to identify recently mobilized transposons in...
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Transcript of Biotechnology. Comparative genomics also has been used to identify recently mobilized transposons in...
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Comparative genomics also has been used to identify recently mobilized transposons in genetically diverse humans. For example, over 600 recent transposon insertions were identified by examining DNA resequencing traces from 36 genetically diverse humans.
Tbl 1 Which transposable elements are active in the human genome? (2007) Ryan E. Mills et al. Trends in Genetics 23: 183-191
EnzymeRecognitionSequence Cut
EcoRI 5'GAATTC3'CTTAAG
5'---G AATT C---3'3'---CTTAA G--- 5'
BamHI 5'GGATCC3'CCTAGG
5'---G GATCC---3'3'---CCTAG G---5'
HindIII 5'AAGCTT3'TTCGAA
5'---A AGCTT---3'3'---TTCGA A---5'
TaqI 5'TCGA3'AGCT
5'---T CGA---3'3'---AGC T---5'
AluI 5'AGCT3'TCGA
5'---AG CT---3'3'---TC GA---5'
Examples of some restriction enzymes…
Areas of DNA from very small samples can be amplified by PCR, and then cut with
restriction enzymes for RFLP analysis.