Bioreactor Design
description
Transcript of Bioreactor Design
Am I Surface Water Scientist ?Am I Surface Water Scientist ?
Specific to Specific to Hydrology, water shed, Hydrology, water shed, pollutionpollution but not limnology but not limnology
Skill towards Environmental Skill towards Environmental Innovations & contribution to this Innovations & contribution to this emerging scienceemerging science
Water quality to aquatic life, Water quality to aquatic life, agricultural use agricultural use and recreation & and recreation & aesthetic purposeaesthetic purpose
Aerobic Submerged Aerobic Submerged Attached Growth Attached Growth
Bioreactor: A Suitable Bioreactor: A Suitable Device for Intermediary Device for Intermediary Wastewater TreatmentWastewater Treatment
Specific Objectives:Specific Objectives:
To assess the organic matter degradation To assess the organic matter degradation efficiency under different solids and efficiency under different solids and salinity proportions in wastewater.salinity proportions in wastewater.
To evaluate ammonia nitrogen removal To evaluate ammonia nitrogen removal capability under submerged condition of capability under submerged condition of bioreactor.bioreactor.
To find out relation between bacterial To find out relation between bacterial intensity and the degree of waste removal.intensity and the degree of waste removal.
Experimental setup for the different Experimental setup for the different particle size experimentsparticle size experiments
Experimental setup for the material Experimental setup for the material comparison experimentscomparison experiments
: Initial conditions of MCE (stone, : Initial conditions of MCE (stone, glass & tire) experimentglass & tire) experiment
Experiment-1 Experiment-2 Experiment-3
COD (mg/L) 2275 2190 4465
BOD (mg/L) 330 760 1506
NH3-N (mg/L) 302 1000 1730
Packed bed depth (cm) 75 90 90
Bacterial Density (cfu/ml) 2.3 * 104 19.2 * 104 300 * 104
Total solids (mg/L) 2400 10400 15200
Total suspended solids (mg/L) 26 200 985
Electrical Conductivity (S/m)
6060 9500 13500
Hydraulic loading (m3 m-2 d-1) 2.0 4.0 4.0
Measuring parameters and instrumentsMeasuring parameters and instruments Measured parameters Measuring instruments
Dissolved oxygen DO meter of model OM-51
Chemical oxygen demand
COD vial and Lambda 20 Spectrometer
Ammonia-nitrogen NH3-N electrode (5002-10C) with multi-meter
model D-53
Total dissolved solid Oven
Total Suspended solid Vacuum extractor and Oven
Electrical conductivity Conductivity meter of model ES-51
pH pH electrode (6066-10C) with multi-meter
Biomass weight Electronic balance
Bio-film thickness Digital caliper
Air flow supply Aerator
Wastewater flow supply Small submergible pump
Sample of prepared agar for Sample of prepared agar for culturing bacteria culturing bacteria
CFU sample collectionCFU sample collection
Bio-oxidation of organic matters in recirculation processBio-oxidation of organic matters in recirculation process
Nitrification trends in recirculation processNitrification trends in recirculation process
Bacterial performance in recirculation processBacterial performance in recirculation process
Overall performance in Third Cycle experimentsOverall performance in Third Cycle experiments
COD% BOD% NH3-N% HC% TS% CFU 104 EC
Initial value 4465 1506 1730 150 15200 300 13500
Mixed tire 53.95 97.17 95.53 47.02 15.30
Large tire 52.86 98.41 85.01 31.90 17.43
Medium tire 56.67 97.79 79.17 29.37 18.91
Small tire 55.04 99.12 90.34 40.87 15.63
Crumb tire 52.31 98.50 75.44 100.00 13.65
Mean 54.63 98.12 87.51 37.29 16.82
STDEV 1.6 0.8 7.0 8.2 1.7
Stone 55.3 97.6 96.8 22.4
Glass 55.0 98.7 93.1 22.6
Tire 55.6 98.6 81.3 23.8
Mean 55.31 98.29 90.39 22.97
STDEV 0.3 0.6 8.1 0.8
ConclusionConclusion
Aerobic submerged bioreactor has Aerobic submerged bioreactor has been proved to be a potential device been proved to be a potential device for secondary treatment of for secondary treatment of wastewater.wastewater.
The rate of remediation of The rate of remediation of wastewater depends on the intensity wastewater depends on the intensity of bacterial growth.of bacterial growth.
High salinity retards the remediation High salinity retards the remediation process i.e. the bacterial growth.process i.e. the bacterial growth.
ConclusionConclusion
The experimental results indicate The experimental results indicate that spherical shaped. i.e. the small that spherical shaped. i.e. the small size tire chips are better for bio-size tire chips are better for bio-remediation for landfill leachate remediation for landfill leachate treatment than rectangular shape.treatment than rectangular shape.
In submergence bioreactor clogging In submergence bioreactor clogging rate is slower than trickling filter rate is slower than trickling filter and clogging can be eliminated and clogging can be eliminated through backwashing .through backwashing .