Biology 22 Drosophila Identification of various phenotypes.
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Transcript of Biology 22 Drosophila Identification of various phenotypes.
Biology 22
DrosophilaDrosophila
Identification of various Identification of various phenotypes.phenotypes.
Reference for Pictures:
Carolina Carolina Drosophila melanogsterDrosophila melanogster Manual Manual (45-2620)(45-2620)
Published: Carolina Biological Supply Published: Carolina Biological Supply CompanyCompany
The life C
ycle
Life Cycle: Drosophila melanogaster
Eggs: contain filaments Eggs: contain filaments
2 molting phases “instars”2 molting phases “instars”
Life cycle is 10 days Life cycle is 10 days
Fruity Fly Egg and Larva
Fruit Fly Pupa and Adult
Identification of:
Gender
Body Color
Wild Type Versus Mutants
Body ColorBody Color
Wild type: BeigeWild type: Beige Mutant: Yellow bodyMutant: Yellow body
Parental phenotypes Experiment: body color
A= female: wt (beige)A= female: wt (beige) B=female: wt (beige)B=female: wt (beige) C=male: wt (beige)C=male: wt (beige) D=female: mutant (yellow)D=female: mutant (yellow) E=male: mutant (yellow)E=male: mutant (yellow)
Fruit Fly Body Color
Sex combs: Male Drosophila
Abdominal segements
7 segments to female abdominal segments7 segments to female abdominal segments
5 segments to male abdominal segments5 segments to male abdominal segments
Male body size is smaller than femaleMale body size is smaller than female
But the “last” abdominal segment is larger in maleBut the “last” abdominal segment is larger in male
Nap TimeTriethylamine
How to anesthetize a fly
How to use the Napper
View the Fruit Fly
You will record class data
After counting the crosses record the data After counting the crosses record the data on the class spreadsheet on the class spreadsheet
3 point test cross: Experiment 2
Experiment 2Experiment 2 3 point test cross3 point test cross
Eye colorEye color Body colorBody color Wing lengthWing length
3 point test cross for:
1. Body Color1. Body Color
2. Wing length2. Wing length
3. Eye Color3. Eye Color
♦♦ Wt vs. Mutant
Beige (bl+) vs. Black (bl)
Long(vg+) vs. Vestigal(vg)
Brick red (br+) vs. brown (br)
Crosses:
Female Female Males Males
Males and female progeny from this F2 cross Males and female progeny from this F2 cross are your 3 point test cross results!are your 3 point test cross results!
Xbl vg bw
bl vg bw
bl+ vg+ Bw+
bl vg bw
There are all sorts of eyes!
Eyes
You will have only red eyes (wt)
Fruit Fly Wings
1866-1945
Parental phenotypes
A= female: wt beige bodyA= female: wt beige body B=female: wt beige bodyB=female: wt beige body C=male: wt beige bodyC=male: wt beige body D=female: yellow bodyD=female: yellow body E=male: yellow bodyE=male: yellow body
Female and Male Fruit Flies