Biofilms, Methylation & Heavy Metal Detoxification in Lyme Disease
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Raj Patel, M.D. Biofilms, Biofilms, Methylation & Heavy Methylation & Heavy Metal Detoxification Metal Detoxification in Lyme Disease in Lyme Disease
description
Biofilms, Methylation & Heavy Metal Detoxification in Lyme Disease. Overview. Biofilms Gastrointestinal physiology Definition of Biofilms Examples Prevalence Treatment B. Heavy Metals Prevalence Signs & symptoms Testing Treatment options Methylation in non-responders C. Conclusion. - PowerPoint PPT Presentation
Transcript of Biofilms, Methylation & Heavy Metal Detoxification in Lyme Disease
Raj Patel, M.D.
Biofilms, Methylation & Biofilms, Methylation & Heavy Metal Detoxification Heavy Metal Detoxification
in Lyme Disease in Lyme Disease
Raj Patel, M.D.
OverviewOverview
A. Biofilms
Gastrointestinal physiology Definition of Biofilms Examples Prevalence Treatment
B. Heavy Metals
Prevalence Signs & symptoms Testing Treatment options Methylation in non-responders
C. Conclusion
Raj Patel, M.D.
B. Biofilms in Lyme Disease
1. GI Physiology: Structure of normal intestinal lining
Raj Patel, M.D.
Raj Patel, M.D.
B. Biofilms in Lyme Disease
1. GI Physiology: Structure of intestinal lining in Gluten intolerance
Raj Patel, M.D.
Raj Patel, M.D.
B. Biofilms in Lyme Disease
1. GI Physiology
Microbial Flora
I. One hundred trillion bacteria in gut comprises 500 different species
II. Disruption early leads to immune problems, allergies, and autoimmunity later
III. Functions: modulates immune system
destroys toxins introduced with food suppress growth of pathogenic bacteria production of key vitamins digestion and absorption of carbohydrates prevention of allergies prevention of inflammatory bowel disease
Raj Patel, M.D.
B. Biofilms in Lyme Disease
2. Definition
a. Community of bacteria and other organisms surrounded by a extracellular polymeric substance (EPS)
b. EPS is composed of DNA, protein, and polysaccharides. Its negative charge attracts Ca/Mg/Fe to strengthen it
c. Organisms within a biofilm can communicate and exchange genetic material.
d. EPS provides resistance to antibiotics requiring 100-1000X higher levels for eradication.
“Testing the susceptibility of bacteria in biofilms to antimicrobial agents .” Antimicrobial Agents and Chemo. Nov 1990
e. EPS provides this organisms protection from UV exposure, pH changes, heavy metal toxicity, and phagocytosis.
Raj Patel, M.D.
B. Biofilms in Lyme Disease
3. Examples of Biofilms
a. Teeth b. Catheters and IV Linesc. Stagnant pools of water, rivers and streamsd. Contact lense. Polluted areasf. Blood (Fry et al)
Raj Patel, M.D.
B. Biofilms in Lyme Disease
4. Prevalence of Biofilms
a. Autism Spectrum Disorders b. Chronic Lyme Diseasec. Chronic Fatigue Immune Dysfunction Syndromed. Fibromyalgiae. Autoimmune Illness
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Raj Patel, M.D.
B. Biofilms in Lyme Disease
3. Examples of Biofilms
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B. Biofilms in Lyme Disease
5. Role of EDTA in Dissolving Biofilm
a. EDTA serves to bind and remove the Ca/Mg/Fe holding biofilms together
b. Staph biofilms could not be eradicated by Vancomycin or EDTA alone. Together the two agents successfully removed the biofilm
(Kim 2005)
c. EDTA and Gentamycin are 1000X more effective at killing Pseudomonas than either agent alone. This effect is completely blocked
when Ca or Fe are added (Banin 2005)
Raj Patel, M.D.
B. Biofilms in Lyme Disease
6. Treatment of Biofilms
a. Enzymes
b. EDTA
c. Antimicrobials Antifungals
d. Fiber Brown Algae Modified Citrus Pectin Activated Charcoal Zeolite
e. Probiotics Prebiotics (fresh fruit, legumes, chicory, FOS, inulin) Supportive nutrients (slippery elm, okra, NAG, ecklona cava, colostrum)
f. Drainage
g. GO SLOW
