Bacteriophage Antibiotic resistant bacteria eXolution
Transcript of Bacteriophage Antibiotic resistant bacteria eXolution
6F, Sambo building ,296, Jungdae-ro, Songpa-gu, Seoul, Republic of Korea, Postal code 05724
Powder
Bacteriophage
Package5 kg / 10 kg pack
SpeciesSwine, Cattle, Poultry
Active ingredient
Dosage
Bacillus subtilisBacteriophage targeting
Salmonella spp.Escherichia coli
Clostridium perfringensStaphylococcus aureus
General dosage : Mix 300~500 g / MT of feedPiglet dosage : Mix 1 kg / MT of feed
IndicationControlling pathogens susceptible to eXolution bacteriophage, Growth promoter
ApplicationSpecies : Poultry, Swine, Cattle | Type : Feed additive powder type
StorageStore in a cool dry place. Avoid direct sunlight
Shelf life1 year after manufacture date
eXolutionBacteriophage
Effective to Biofilm
Other advantages of Bacteriophage
Bacteriophage can destroy and penetrate the biofilm, so that not only bacteriophage
but also antibiotics can reach to bacteriainside biofilm.
Bacteriophage can control antibioticresistant bacteria.
Because of difference in mode ofaction between bacteriophage and antibiotics,
chance of resistance is very lowwith bacteriophage.
Biofim
AntibioticAntibiotic
Bacteria
“PAS effect”Phage-AntibioticsSynergistic effect
Bacteriophage attacks bacteria with specific receptor only.Therefore beneficial bacteria such as Lactobacillus are safe from eXolution andit will help health of livestock.
- No side effect according to dosage
- No withdrawal time
Effective toAntibiotic resistant bacteria
* PAS : Phage Antibiotic Synergy
Bacteriophage
eXolutionWhat is Bacteriophage?
How Bacteriophage kills Bacteria?
Challenge trial
·Natural material - Most abundant in nature, 1031 in earth, can be found in everywhere
·Safe to human and animal – Attack only target bacteria
·Have bacteria as a host, much smaller than bacteria
·Found prior to antibiotic discovery (1896)
Layer Egg productioneXolution
SwineeXolution
BroilereXolution
Harmful Microflorachange in feces
eXolution
Harmful Microflorachange in organs
eXolution
- Egg production, % -
0 to 3 WK 4 to 6 WK
90.892
89.9
92.1
Con eXolution
- Bacteria Population, Log10 cfu/g -
- Bacteria population, Log10 DNA copy numbers/g -
E. coli Salmonella
6.756.28
3.472.11
Con eXolution
1.566
1.5411.528
FCR
1.62
1.55 1.56
FCR
Con Avilamycin eXolution
5.665.27
4.89
Salmonella
Con 0.04% eXolution 0.08% eXolution
Con BMD eXolution
Items Con Apramycin eXolution
G/F
Dry matter, %
Nitrogen, %
Energy, %
0.357
77.40
77.00
76.63
0.377
80.08 *
78.43
79.22
0.371
79.57 *
79.22 *
78.46
Items Con eXolution
ADG, g
ADFI, g
FCR
737
2,079
2.82
764
2,129
2.79
Log10 cfu/g Con Apramycin eXolution
E.Coli
Salmonella
6.55
3.62
6.00 *
2.57 *
6.14 *
2.02*
Fecal shedding Log10 cfu/g Con Sal. Enteriridis SE+0.2% eXolution
No. at weaningPiglets / litter, kg
BW at weaningPiglets / litter, kg
ADG, g / pig
Sow feed without eXolution
eXolution eXolution- -Creep feed
10.1
56.8
234
10.4
65.1
260
10.2
57.6
235
10.6
65.3
258
eXolution
SE+0.1% eXolution
3 dpi 0 3.45
1.58
3.17
1.58
2.80
0.7106 dpi
Phage Attaches to receptor site onbacterial cell wall,
penetrates it, and insert its DNA.
The phage DNA directsthe cell’s metabolism
to produce viral components-proteinsand copies of phage DNA.
Empty phage headsare synthesized.
Heads are packedwith DNA.
Collars, sheaths, and base plateshave been attached to heads.
Tail fibers are added last.
Bacterial cell lyses,releasing completed
infective phages.
* FCR : Feed Conversion Ratio
Nucleus : 2,800nmNucleus : 2,800nm
E.coli : 2,000nmE.coli : 2,000nm
Phage : 95nmPhage : 95nm
Eukaryotic cell : 10,000nmEukaryotic cell : 10,000nm