15

20

25

30

35

40

The highest temperature

The lowest temperature

Mean temperature in one day

Supplemental Fig. S1 Greenhouse temperature during the experiment. The highest, lowest, and mean temperatures for each day are indicated

ºC

Date in 2008

L2 L15 L19 L22

0 0 2 0.3

Supplemental Fig. S2 Appearance of F3 lines. Each F3 line contains of 12 plants (two rows of six plants) grown in the inoculated soil medium. Line identifiers (above) and mean DIs for each line (below) are indicated

Supplemental Fig. S3 The electrophoresis pattern of PCR products generated from the KBrS003O10N1 marker. DNA samples were derived from ‘GC’, ‘Anju’, and F3 plants. Scored genotypes for the KBrS003O1N10 maker are indicated below each lane (A: ‘GC’, B: ‘Anju’, H: Heterozygous). Inoculation test phenotypes (DI scores) are also provided below each band. Arrows indicate parental plants.

GenotypePhenotype

KBrS003O10N1

400bp

300bp

AnjuGC

A2

B0

B0

H1

H0

H0

B0

A2

H1

A2

H0

H0

H1

A2

A2

H0

Supplemental Fig. S4 Synteny map of the Foc-BoI region with A. thaliana and B. rapa. The chromosome numbers are given upon each vertical bars. The markers of B. oleracea that are homologous with A. thaliana genes or B. rapa BAC clones are connected by broken lines. KBrS003O10N1 that is closest to Foc-Bo1 is given in bold. KBrB089H07N1 that is closest to pbBo(Anju)4 is underlined

100 kb

At4g24330

At4g20150

At4g15232At4g16070

At4g22930

At3g51910At3g51410

pW166

KBrB089H07N1

TCR05

KBrS003O10N1

BSA8

KBrB084F01N1KBrS012D09N

Foc-Bo1

1 cM

KBrB089H07

KBrS003O10

KBrH005A08

KBrB084F01

KBrS012D09

2 cMB. oleracea (O7) A. thaliana (At3 & At4) B. rapa (R3)