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Arsenic in Livestock Well Water on the Din Reservation Clarita Lefthand, Ph.D. Student University of Washington Some Data presented here was obtained from the Navajo EPA

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Prevalence of Arsenic and Some Background

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Naturally occurring element in bedrock Have no smell and no taste. Difficult to tell if arsenic is present in food, water, or air

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Anthropogenic Sources Smelting of metals Pharmaceutical industry (medicines) Pesticide manufacture (very limited) Wood preservative Cattle and sheep dips Petroleum, coal, and wood burning Waste incineration

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Counties with arsenic concentrations exceeding MCL in 10% or more groundwater samples.

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Some Current Sampling Data Of 199 sources tested for inorganic compounds: 44 (22%) exceeded one or more of the primary drinking water standards The most frequent exceedance was arsenic (24 [12%] sources) followed by uranium (9 [5%] sources). The highest arsenic level was 190ug/L Of 188 water sources tested for bacteria: 40 (21%) were positive for E. coli and 144 (77%) were positive for total coliforms

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Hauled Drinking Water Sources N responses = 94 (N total =296) Multiple sources could be used by same individual Livestock Well 30% Chapter House 22% Natural Spring 11% Did Not Specify 37% Livestock Well Chapter House Natural Spring Did Not Specify or Other

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Hazard Identification

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Water Soluble Arsenic Species

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Organic Arsenic Less toxic than inorganic As Produced by biomethylation Microorganisms in soil and water Humans detoxify organic As

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Mode of Action Cancer: The most accepted explanation of the mode of action for Ar carcinogenicity is that it induces chromosomal abnormalities without interacting directly with DNA Non-cancer: Ar has inhibitory effects cellular respiration at the level of the mitochondrion. Oxidative stress might also have an important role in both cancer and non-cancer effects

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Toxicokinetics

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Absorption Soluble forms are well absorbed in the GIT by humans 60%-90% Animals 50% Insoluble forms Limited absorption

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Distribution After absorption Ar initially accumulates in the Nails Hair Bone Kidney, liver, lung Binds to sulfhydryl containing proteins - concentrates in the hair and fingernails

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As 5+ (Arsenate) As 3+ (Arsenite) Methylarsenite (liver) Dimethylarsenite (readily eliminated urine) Metabolism of Inorganic Arsenic Ar undergoes methylation to less toxic metabolites

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Excretion Half-life= 3-5 days Primarily via urine

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Health Effects of Chronic Ingestion Cancers: Bladder, kidney, liver and skin cancers: drinking water Lung cancer caused from inhalation Non-Cancer Endpoints: Blackfoot disease Increases a persons risk of vascular and heart disease, type 2 diabetes, reproductive and developmental disorders, low birth weights in babies, neurological problems and lower IQs in children. neurological, renal, cardiovascular, hematological, and testicular effects.

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Epidemiology Studies Taiwan: The most studied area Chile: Argentina: In these studies chronic exposure was determined to cause bladder, lung and skin cancer [Ar] were several hundred micrograms per liter EPA cancer risk assessment has used the cancer data from SW Taiwan (primarily bladder cancer cases in the Blackfoot-Disease endemic area) to predict the cancer risk from arsenic in drinking water in the USA

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Exposure Assessment

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Routes of Exposure Ingestion The most important route of exposure because it does the most damage Skin Contact Also a very important route of exposure Inhalation A minor pathway of exposure

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Exposure among Din Communities In this assessment we are primarily concerned with ingestion of Ar Current data suggest that exposure occurs mainly from the ingestion of arsenic through contaminated livestock well water Adverse health effects depend on dose, duration of exposure, and the nutritional state of exposed populations In the case of the Din community, chronic exposure is of concern

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Ar Concentrations Measured in Din Communities CommunityConcentration of Arsenic (mg/L) Sweetwater (09T-592)0.026 Red Mesa (9T-538)0.05 Lower Greasewood0.17 A lot of communities~0.02

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Standards for Arsenic in Drinking Water AgencyLevelComments World Health Organization (WHO) 0.01mg/L is the allowable concentration Inorganic arsenic is a documented human carcinogen. Environmental Protection Agency (EPA) -- 0.01 mg/ml is the maximum contaminant level -- zero mg/ml is the MCLG -- RfD=0.3 ug/kg/day EPAs stated policy in setting MCLs for known human carcinogens has the goal of ensuring that the MCL falls within the 1: 10,000 to 1: million range

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Exposure Assessment Ingestion (mg/kg/day) =(C*IR*ED*EF) BW*AT C: Concentration = 0.17 mg/L IR: Ingestion Rate =0.927L/day ED:Exposure Duration = 75 yr EF: Exposure Frequency= 365 days/yr BW: Body Weight= 70 kg AT:Averaging time= 75 yr*365 day=27,375 d

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Exposure Assessment Ingestion =0.17 mg/L*0.927L/day = 70 kg Ingestion= 0.0023 mg/kg/day EPA RfD=0.3 ug/kg/day=0.0003 mg/kg/day The average adult persons exposure is 8 times higher than the EPAs RfD

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Exposure Assessment Limitations Other exposure questions that must be address include: Is exposure from food ingestion possible? Arsenic may also be released into the atmosphere from coal-fired power plants and incinerators because coal and waste products often contain some arsenic Soil consumption by children Moreover what about childrens or pregnant mothers Dose?

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Precautionary Assessment Community/Social Issues Exposure Issues Hazard/Toxicity

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Precautionary Assessment Community/Social Issues ParameterScore Goal2 Need3 Future Generations3 Democratic Community Based Process2 Alternatives3 Total13/15 A score of 13 suggests that at present there is very little support for health or community Note: Please see end of presentation for more detailed information about Evaluation Scores

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Precautionary Assessment Exposure Issues ParameterScore Exposure3 Multiple Exposures3 Children Exposed5 Consumer Products (Sheep Meat)2 Occupational Exposure1 Food Exposure2 Total16/20 Note: Please see end of presentation for more detailed information about Evaluation Scores A score of 16 suggests that there is an exposure problem that presents concerns to the community

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Precautionary Assessment Hazard/Toxicity ParametersScore Hazard10 Individual Sensitivity3 Ecological Hazard2 Volume5 Persistent3 Bioaccumulate1 Uncertainty3 Total27/30 A score of 27 suggest that there is a significant hazard that present a serious concern Note: Please see end of presentation for more detailed information about Evaluation Scores

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Conclusions Exposure Assessment Ingestion= 0.0023 mg/kg/day EPA RfD=0.0003 mg/kg/day The average adult persons exposure is 8 times higher than the EPAs RfD. Precautionary Assessment Community and Social Issues: 13/15 Exposure Issues: 16/20 Hazard and Toxicity: 27/30 In essence the [Ar] present in well water on the Din do not strongly promote human health, a sustainable environment, and poorly ensures that future generations of all species have an opportunity to thrive

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Risk Management Continue to monitor and sample for all the livestock wells It would be useful to have a better understanding about other exposures: i.e., Sheep meat, soil ingestion, and air contamination Consider how the nutritional state of the exposed Din population might impact individual health Determine long-term strategies for for drinking water issues including those that extend beyond arsenic contamination For example: fecal coliform and uranium; and lack of drinking water for future

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Risk Management Remediation Point of use: daily consumption of water Would need to be modified for water haulers!

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Risk Management Remediation Point of use: daily consumption of water Would need to be modified for water haulers!

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Risk Management Need plans that will extend water lines to Din homes Need to establish a safe water hauling point Ask Community what they believe is the best approach to remediating water If the community is not involved, funds may be used to create a solution that is not culturally viable

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Risk Communication Help educate the community who are directly impacted by contaminated water about their potential exposure to Arsenic and the adverse health risks Arsenic in water is invisible, does not smell, and has no taste Inform the community in a culturally appropriate way Continue and encourage community involvement in the decision making process to clean up the well water or to find alternative water sources that are viable for the community

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References NRDC http://www.nrdc.org/water/drinking/qarsenic.asp http://www.nrdc.org/water/drinking/qarsenic.asp EPA http://www.epa.gov/safewater/arsenic/index.htm l http://www.epa.gov/safewater/arsenic/index.htm l Toxmetal http://www.dartmouth.edu/~toxmetal/research/ projects/Arsenic.html http://www.dartmouth.edu/~toxmetal/research/ projects/Arsenic.html

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Precautionary Assessment Community / Social Issues Community / Social Issues Score Evaluate effects on the community and related social issues. Goal1-3 1-a lot, 2-some, 3-little. Does this move forward the goal of human and environmental health? Need1-3 1-a lot, 2-some, 3-little or not sure. Ask the question: Is it necessary? Do we really need this? Future Generations1-3 1-little, 2-some, 3-high impact. Is there a potential impact on future generations of humans and other species? Democratic, community based process1-3 1-a lot of community involvement and consultation, 2-some, 3-little. Was the community consulted early and often in the process? Was the process democratic and inclusive. Alternatives1-3 1-alternatives were carefully considered, 2-some consideration, 3-no consideration. Where alternatives considered? Total5-15 5-good, supportive of health and community 15-poor, not supportive of health or community

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Precautionary Assessment Exposure Exposure IssuesScore Evaluate Potential Exposure Issues Exposure0-3 0-none, 1-little, 2-some, 3-high. Do we have control over the exposure? Multiple exposures0-3 0-none, 1-little, 2-some, 3-high. Is there exposure to other chemicals with similar hazard? Children exposed0,3,5 0-none, 3-little, 5-some or high or don't know. Children are often more vulnerable. Are children being exposed. Consumer products0-3 0-not in consumer products, 1-little, 2-some, 3- a lot or do not know. Is this compound in consumer products? Occupational exposure0-3 0-no occupational exposure, 1-little, 2-some, 3- a lot or do not know. Is there occupational exposure? Food exposure0-3 0-not in food supply, 1-little, 2-some, 3- a lot or do not know. Is the compound present in the food supply. Total0-20 0-no exposure, no problems 20-significant exposure, serious concern

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Precautionary Assessment Hazard / Toxicity ScoreEvaluate Potential Hazards Hazard1,5,10 1-low, 5-some, 10-high. Follow classical hazard evaluation, pick endpoint, exam relevant quality studies (cancer, reproductive, neurotoxicity, irreversible) Individual Sensitivity1-3 1-little 2-some, 3-a lot. Determine if any individuals are more sensitive than health adult such as the very young or old. Ecological hazard1-3 1-little 2-some, 3-a lot. Is it a hazard to other species or the environment? Volume1-5 how much is produced (1=research only, 2= 100,000 or do not know) Persistent1-3 1-little persistence 2-some, 3-a lot of persistence or do not know. Is the compound persistent in the environment? Bioaccumulate1-3 1-little 2-some, 3-a lot. Does it bioaccumulative in humans or animals or move up the food chain? Uncertainty1-31-little 2-some, 3-a lot. How certain is the information? Total7-30 7-low hazard 30-significant hazards or unknowns, serious concern

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