ars.els-cdn.com · Web viewSupplementary Material on. Marshmallow-like silicone gels as flexible...
Transcript of ars.els-cdn.com · Web viewSupplementary Material on. Marshmallow-like silicone gels as flexible...
Supplementary Material on
Marshmallow-like silicone gels as flexible thermal insulators and liquid
nitrogen retention materials and their application in containers for
cryopreserved embryos
Gen Hayase* and Yasutaka Ohya
Movie S1 Time-lapse movie of the sample MG-15 during evaporative drying. One second in
this movie is equal to 30 min of actual time.
Movie S2 Reconstructed image of microCT tomography of the sample MG-15 (See also Figure
2c).
Movie S3 The surface of the sample MG-15 repelling water.
Fig. S1 Microscopic image of two-cell stage embryos. From the shape, it can be determined
that the embryo indicated by the arrow is dead.
MG-25 MG-30
MG-40MG-35
MG-50 MG-60
MG-80 MG-100
Fig. S2 Particle size distribution of the MGs.
Fig. S3 SEM images of the marshmallow-like gels (a) MG-5, (b) MG-10, (c) MG-15, and (d)
MG-20 prepared by increasing urea by 20 % in the starting composition. There was no clear
change due to the increase in urea (see also Fig. 2).
MG-25 MG-30
MG-40MG-35
MG-50 MG-60
MG-80 MG-100
Fig. S4 Particle size distribution of the MGs prepared by increasing urea by 20 % in the starting
composition. There was no clear change due to the increase in urea (see also Fig. S2).
Fig. S5 Thermographic image during evaporation drying of the marshmallow-like gel MG-15.
The wet gel during evaporation kept the temperature lower than the heat source.
Fig. S6 (a) Photographs of cloths (cut into ~10 mm square) and the container with polyester
(PE).
Table S1 Number of surviving embryos in each experimental test.
Sample Tria
l
Time
[min, min, min]
Number of frozen/thawed
embryos
Number of surviving
embryos
2-cell stage 4-cell stage
MG-15 1 60, 120, 180 40, 40, 40 39, 40, 40 36, 36, 35
2 60, 120, 180 39, 40, 40 39, 39, 40 37, 36, 37
3 60, 120, 180 39, 40, 40 38, 40, 40 35, 36, 38
1 180, 360, 540 40, 40, 39 40, 40, 39 38, 35, 36
2 180, 360, 540 40, 40, 40 40, 40, 39 35, 37, 36
3 180, 360, 540 40, 39, 40 40, 39, 40 39, 38, 36
PE 1 60, 120, 180 40, 39, 40 40, 38, 39 38, 34, 37
2 60, 120, 180 40, 40, 40 40, 40, 40 36, 37, 36
3 60, 120, 180 40, 39, 40 40, 39, 40 37, 36, 36
1 180, 360, 540 40, 39, 40 40, 0, 0 37, 0, 0
2 180, 360, 540 40, 39, 39 39, 0, 0 35, 0, 0
3 180, 360, 540 40, 39, 38 40, 0, 0 36, 0, 0