ARMI Stakeholders Extending internationally 04 01 01 12 31 ... Report_2012... · 6 Page ARMI AnnuAl...

ARMI AnnuAl RepoRt 2012

ARMI Stakeholders

Extending internationally

0401 01 12 31 12 12

Australian Regenerative Medicine Institute2012 Annual Report

editorial teamlaura Crilley Silvio tizianiMelissa trudinger, Science in PublicMargie Beilharz, Science in Public

designlinda Cerkvenik, outsource Design

photographyDavid Russell, David Russell Photography

Printed on 100% recycled paper

trademark – Australian Regenerative Medicine Institute 2012

Acknowledgements

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ARMI AnnuAl RePoRt 2012

Introduction 2Director’s Report 3About the Australian Regenerative Medicine Institute (ARMI) 4

Research 62012 Highlights 7Appointments 9ARMI Scientifi c Retreat 11eMBl Australia 12SBI Australia 15Rosenthal Group 16Currie Group 18Barberi Group 20Bourne Group 22Heng Group 24Hobbs Group 26Kaslin Group 28lieschke Group 30Marcelle Group 32McGlinn Group 34Plachta Group 36Polo Group 38

Core Research Facilities 40FishCore 41Gene Recombineering 42eS Cell Genetics Services 43ARMIRat 44FlowCore 45Micromon 46Monash Micro Imaging 47Structural Biology 48Monash Gene targeting Facility 49Monash Antibody technologies Facility 50

Governance 52Committees 53ARMI team 58nationalities 60

Financials 62

Activities 64Visitors 65events 66ARMI in the news 67external Seminar Series 68Internal Seminar Series 69Research Activities 71BioeYeS outreach Program 84Publications 85How to donate to ARMI 90

Contents

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IntroductIon

LookIng outwards, strong natIonaL and InternatIonaL LInkages are a key strategy for contInuIng to Improve our scIentIfIc outputs.

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Highlights from our fourth year of operation include the ARMI Scientifi c Retreat, held at Silverwater Resort near Phillip Island, which gave our research staff the opportunity to review and discuss their research programs with their peers. We were delighted that Professor Peter Rigby and Professor Dame Kay Davies, both eminent members of our newly established Scientifi c Advisory Board, attended the retreat and welcomed the feedback and recommendations they generously provided.

our Institute grew by almost 50% with the addition of new postdocs, PhD and undergraduate students and technical staff. At the end of 2012 we had 12 research groups.

two key indicators of successful research are the number of grants awarded to support the continuation of the research and number of research papers published. on both of these indicators, the Institute has had a successful year. twelve out of 32 grant applications submitted to the national Health and Medical Research Council (nHMRC) and the Australian Research Council (ARC) were successful. this 38% success rate is well above the national average of around 20%. In 2012, ARMI scientists published almost double their 2011 output, an indication that our research programs are maturing. It was exciting to see that the number of papers published in high impact journals including Cell and Science also increased signifi cantly.

We have also recognised one of our group leaders, James Bourne, for his outstanding achievements both as a researcher and also through his efforts to develop our accredited Higher Degree by Research (HDR) program by promoting him to Associate Professor.

our student programs have continued to expand, with 11 students enrolled in higher degrees and six in Honours. And all six PhD students from our fi rst HDR cohort achieved candidature in 2012.

looking outwards, strong national and international linkages are a key strategy for continuing to improve our scientifi c outputs. In 2012 we appointed two new adjunct professors: Justin Cooper-White and Francis Stewart. Professor Cooper-White at the Australian Institute for Bioengineering and

nanotechnology in Queensland is an internationally renowned expert in bioengineering tissue scaffolds and smart surfaces, and his appointment will add a new dimension to our regeneration research. Professor Stewart, at BioInnovationsZentrum, technische universitaet Dresden in Germany, has played a role in developing many of the recombinant DnA technologies we use at ARMI and has recently begun to focus on systems biology. We welcome them both to ARMI and hope their visits prove fruitful for all of us.

eMBl Australia, which is headquartered here at ARMI, is continuing to grow and develop its programs, with the launch this year of a suite of student travel grants and programs, and plans for a new node at the new South Australian Health and Medical Research Institute well underway.

our newest initiative is SBI Australia, the fi rst international node of Japan’s Systems Biology Institute. SBI Director, Hiroaki Kitano, was appointed Sir louis Matheson Distinguished Visiting Professor at Monash university last year, with an adjunct appointment at ARMI, and is already collaborating with several groups here. With our new links through SBI Australia, we are looking forward to a long and fruitful association with SBI.

through SBI Australia and our close association with eMBl Australia, we are showcasing Australia’s life sciences research to the world.

All of our successes over the last year would not have been possible without the hard work of our ARMI research teams, staff and students. In the coming year, our focus will be on strategic planning to maintain our trajectory for sustained leadership in the global regenerative medicine landscape.

Professor Nadia RosenthalFounding DirectorAustralian Regenerative Medicine Institute

Director’s ReportI am pLeased to report that 2012 has been another year of successfuL growth and deveLopment for the austraLIan regeneratIve medIcIne InstItute (armI).

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through a joInt venture between monash unIversIty, the government of vIctorIa and the federaL government, armI has been estabLIshed as a state-of-the-art regeneratIve medIcIne research facILIty at the cLayton campus of monash unIversIty In meLbourne. buILdIng on the unIversIty’s exIstIng strengths In bIomedIcaL and stem ceLL research, crItIcaL Infrastructure (IncLudIng LaboratorIes and core equIpment) has been assembLed to deLIver the next generatIon of research outcomes In regeneratIve medIcIne. austraLIa’s fIrst regeneratIve medIcIne research centre Is a tImeLy response to capture new opportunItIes In a rapIdLy expandIng sector of bIomedIcaL research. the InstItute boasts a cross-dIscIpLInary, hIghLy focused approach to the scIence of regeneratIon, desIgned to seed and foster coLLaboratIon, and to pursue rapId transLatIon of basIc research Into cLInIcaL treatments.

Central to ARMI is the attraction and development of future scientific leaders, recruited through the eMBl Australia Group leader pipeline. these young researchers of high potential are provided guaranteed research funding for a defined period to accelerate their careers and nurture their talent. expert mentoring, combined with state-of-the-art core research infrastructure and facilities, provides a rich and inspirational research environment. the practice of Group leaders moving on to other institutes after a flying start enhances ARMI’s flexibility and disseminates this dynamic research culture to other institutions.

ARMI scientists are devoted to addressing questions in regenerative biology that provide the knowledge base to move beyond current therapies into combination therapy paradigms. the Institute’s Faculty members, recruited from around the world, represent a broad range of disciplines that contribute to a shared vision for the development of regenerative therapies, capturing new opportunities for international collaboration. the functional integration of key research platforms at each level of enquiry—molecular genetics, stem cell biology, and animal modelling—aims to deliver technologies with medium-to-long term application for treatment of diseases of social, medical and economic importance to Australia; and promises to redefine how regenerative medicine is approached worldwide.

ARMI could not have been established without significant financial contributions from both Monash university and government. Monash university’s capital investment of $103 million for the construction and fit-out of new state-of-the-art laboratory facilities was augmented by a contribution of $50 million from the Victorian and Federal Government for additional ARMI laboratory fit-out and the acquisition of critical research equipment. the capital cost of the $100 million ARMI project included provision for the construction of the nearby Buildings 76 and 77 to house the research laboratories of the Faculty of Medicine’s School of Biomedical Sciences.

About the Australian Regenerative Medicine Institute (ARMI)

IntroductIon

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vision the Australian Regenerative Medicine Institute at Monash university is a globally oriented research organisation committed to discoveries and therapeutic strategies that lead to restoring health and normal function, repairing damaged tissues and regenerating the ageing body.

mission ARMI’s mission is to:• promote Australia as a global leader in

regenerative medicine and assist Melbourne to become one of fi ve major biotechnology centres in the world

• foster and develop an ethos of collaboration across Melbourne to link areas of existing excellence and accelerate clinical results

• enhance research excellence by building linkages with research users and providers against the backdrop of Monash stem cell science and biomedical research, enhancing international collaborative endeavours

• contribute to undergraduate teaching programs and establish a major site for postgraduate training.

themesRegenerative Medicine: Regenerative Medicine seeks to repair, replace, restore and regenerate tissues and organs damaged by age, injury, and genetic and degenerative conditions. It represents a revolution in human health, and has the potential to reverse tissue damage, repair traumatic injuries and improve the health of an ageing population.

Outstanding Science: ARMI actively recruits young, creative scientists from all corners of the world, to share and inspire differing approaches to some of the most perplexing biological questions of our time. they are highly motivated, nurtured in a collaborative working environment and approach complex biological problems with ingenuity and passion.

Internationalism: ARMI’s standing as secretariat and headquarters of the esteemed european Molecular Biology laboratory (eMBl) Australia makes the Institute a portal to the global international community; providing researchers unique access to the best science in europe and a new way of approaching scientifi c endeavour. SBI Australia, the fi rst international node of the renowned Systems Biology Institute in Japan, is also being hosted by ARMI and provides valuable links to Japan.

Research Ecosystem: As a global biotech life science centre situated at Monash university, ARMI is an integral presence in the broader Melbourne research and medical ecosystem. Its state-of-the-art core scientifi c facilities are maintained by outstanding infrastructure and technology.

Community: ARMI is a signifi cant part of the fabric of Melbourne through its outreach programs and engagement with community.

Passport: Working side by side with research groups from eMBl Australia enriches scientifi c development and fosters new international research collaboration.

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research

our InstItute grew by aLmost 50% wIth the addItIon of new postdocs, phd and undergraduate students and technIcaL staff. at the end of 2012 we had 12 research groups.

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• At 31 December 2012, ARMI has 146 people, comprising researchers, students, affi liates, core facility support and administrative personnel.

• ARMI’s grant application success rate of 38% for the year was higher than the national average of just over 20%. total income from grants was more than $4.8 million, an increase of 18% over the previous year.

• ARMI researchers published 53 papers (up from 26 in 2011) as well as four book chapters and one book. Highlights include papers in the high impact journals Cell and Science.

• the number of students enrolled in ARMI’s programs increased signifi cantly. there are now 11 PhD Students enrolled in the ARMI Higher Degree by Research (HDR) program, up from six. In addition, the Institute had six Honours students and six undergraduate students through the undergraduate Research opportunities Program (uRoP).

• two new adjunct professors joined ARMI’s professorial group: Professor Justin Cooper-White from the university of Queensland’s

Australian Institute of Bioengineering and nanotechnology and Professor Francis Stewart from the BioInnovationsZentrum, technische universitaet Dresden, Germany.

• ARMI’s international linkages were further strengthened by the Systems Biology Institute’s decision to open its fi rst international node at Monash university. SBI Australia is hosted at ARMI.

• the Institute held a scientifi c retreat at Silverwater Resort near Phillip Island in May, attended by all staff members as well as the Scientifi c Advisory Board, to review research programs.

• eMBl Australia continued to grow and develop, launching a student travel grants program, and establishing the Australian Bioinformatics network, in partnership with CSIRo and BioPlatforms Australia. Plans to develop a new node in South Australia were also announced.

• Plans for the construction of a new aquatics facility to house axolotls and sharks progressed well with completion and commissioning of the new facility expected to be mid-2013.

2012 Highlights

adm

in

acad

emic

tech

nica

l

stud

ent

affi l

iate

tota

l

Dec-08 4 4 3 0 0 11

Jun-09 6 12 12 6 0 36

Dec-09 8 25 15 7 1 56

Jun-10 8 25 16 13 2 64

Dec-10 10 29 25 13 1 78

Jun-11 8 31 30 18 3 90

Dec-11 7 34 39 15 5 100

Jun-12 7 39 42 25 13 126

Dec-12 8 46 46 32 14 146

armI staff, students and affi liates

Dec–08 Dec–09 Dec–10 Dec–11 Dec–12

affi liate

student

technical

academic

admin

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publications

grants success rate

funding source

number of applications

number successful

success rate

nHMRC 22 9 41%

ARC 10 3 30%

total 32 12 38%

research

competitive grant income ($ million)

2011 2012

journal papers 25 53

book chapters 2 4

books 0 1

total no of publications 27 58

papers in journals with impact factor > 5

48.0% 45.8%


28.0% 16.7%


4.0% 10.4%

academic staff 34 46

journal papers per academic

0.7 1.0

journal papers with an impact factor > 10 per academic

0.4 0.5

2009 2010 2011 2012

4.09

0

1.3

63

1.192

4.83

1

2011 2012

>20

10–20

5–10

<5

publications in journals of different impact factor

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promotionARMI team leader James Bourne was promoted to Associate Professor in December 2012. this promotion arises from James’s steady research output, student supervision and the development and coordination of the Institute’s Higher Degree by Research (HDR) program.

James, who has been at ARMI since 2008, has an international reputation for his research in the fi eld of development and regeneration of the visual system. He is particularly interested in teasing out the molecular processes of degeneration and regeneration of the primate neocortex.

His research takes a “cell-to-system” approach, looking at the pathways, mechanisms and factors underlying how the human brain processes the rich visual environment it perceives; at the cellular level as well as within the system as a whole. It’s an approach that has granted James and his team great insights into how the primate visual cortex has evolved, as well as mechanisms of neuroplasticity in the developing brain.

ultimately, he hopes to leverage this knowledge into therapies for injured brains that assist or enable better repair and greater functional recovery.

study raises hope for stroke treatmenttherapy to mend parts of the brain damaged by strokes has moved a step closer, thanks to research performed by Associate Professor James Bourne and his team in collaboration with scientists from at the Florey Institute of neuroscience and Mental Health.

James, with ARMI’s Dr Jihane Homman-ludiye and Dr tobias Merson of the Florey, have discovered precursor cells, in the visual processing region of the brains of young marmoset monkeys, that can form new brain cells in a culture dish.

the work, which was published in April 2012 in the journal PloS one, raises the possibility of new therapies for victims of brain injuries such as stroke.

the team exposed cells obtained from biopsy samples to various combinations of growth factors—proteins that promote cell proliferation—to see if they would multiply and form neurons in the culture dish.

When treated with two specifi c growth factors, some of the cells started to multiply to form clusters of cells called neurospheres, which are the forerunners of mature brain cells and thus in a class of cells called neural progenitors. like stem cells, these cells can convert into specialist cells to form various tissues.

the results suggest that precursor cells with the ability to form new neurons after birth are much more widespread in the brain than previously thought. the cells under investigation in this latest research were isolated from the primary visual cortex, the brain structure at the back of the head involved in the processing of stimuli from the eyes. It’s an area of the brain that is often affected by brain injury, such as the damage caused by an ischemic stroke.

Further research is underway to see if the production of new neurons after birth occurs naturally in the primary visual cortex, and whether the mechanism can be activated after injury.

Appointments

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adjunct appointmentsIn 2012, we continued our program to develop strong international linkages with leading researchers. two new highly ranked international researchers agreed to adjunct appointments to the Institute.

professor justin cooper-whiteGroup leader, Australian Institute of Bioengineering and nanotechnology

Professor Justin Cooper-White is a global leader in using engineering to solve problems in biology. He currently holds positions of Associate Dean (Research) of the Faculty of engineering, Architecture and It; Professor in the School of Chemical engineering and Group leader within the Australian Institute for Bioengineering and nanotechnology—all at the university of Queensland. He is also Director of the Queensland node of the Australian national Fabrication Facility.

Justin’s current research interests include the development of smart surfaces, scaffolds and diagnostic microdevices for stem cell expansion, tissue engineering and early disease detection. He has published more than 250 research papers, book chapters and presentations and has seven international patents in the areas of formulation design for agriproducts, microbioreactors, particle synthesis using microfluidic devices and tissue engineering scaffolds.

Justin is the past President of both the Australasian Society for Biomaterials and tissue engineering and the Australian Society of Rheology; a consultant for a number of national and international companies; associate editor of the Korean-Australian Rheology Journal; on the editorial boards of Soft Materials, Biomicrofluidics and Rheologica Acta; and a reviewer for major international journals in his fields of expertise.

Justin is developing several collaborative projects with ARMI Director nadia Rosenthal.

professor francis stewartProfessor of Genomics, BioInnovationsZentrum, technische universitaet Dresden, Germany

Francis Stewart has held his position as Professor of Genomics at BIoteC interdisciplinary research centre at the technische universitaet, Dresden, since 2001. After completing his undergraduate and postgraduate studies at the university of new South Wales, and postdoctoral studies at the German Cancer Research Centre, he spent nine years as a Group leader at eMBl Heidelberg.

Since moving to Dresden he has been highly influential by developing high-throughput recombineering in genome-scale applications. His innovative protein tagging approaches for systems biology have been the basis of the eu 7th Framework integrated project SyBoSS (Systems Biology of Stem Cells and Reprogramming, www.syboss.eu) which he coordinates. Francis was made an eMBo Fellow in 2007 and was awarded the International Society for transgenic technology Prize in 2010. He has also a held a guest Group leader position at the Max Planck Institute for Cell Biology and Genetics in Dresden.

Francis is an internationally recognised scientist with breakthroughs in the epigenetic mechanisms in mammalian embryogenesis and in developing innovative methods to modify gene expression in mice. He has recently initiated a systems biology approach to understand the stem cell transitions focusing on neural stem cells. In future, he will focus on epigenetic regulators to define the circuitry of each stem cell state and to infer how the transitions are accomplished. In particular, Francis will now focus on the role of epigenetic mechanisms in the establishment of, and exit from, naïve pluripotency. After ten productive years in Dresden, Francis is looking for new challenges while maintaining continuity and building on his current activities, and would like to strengthen his relationship with Australian bioresearch through this appointment.

research

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In late May 2012, students, postdocs, team leaders, core facility and support staff gathered at the Silverwater Resort near Philip Island for the three-day retreat. the retreat was also attended by two members of ARMI’s newly established Scientifi c Advisory Board: Professor Peter Rigby (the Institute of Cancer Research, Royal Cancer Hospital, london) and Professor Dame Kay Davies (oxford university). Professors Rigby and Davies provided valuable feedback to group leaders and researchers on the direction and progress of their research programs.

over the three days of the retreat, research programs were presented, discussed and dissected in a series of presentations by group leaders and team members.

In a report to ARMI’s senior management team, Professor Rigby and Professor Davies commended the scientists for their novel research programs, use of the available core facilities and services and willingness to collaborate. they noted that “overall, the Institute has made an excellent foundation on which to build for the future. there are real opportunities arising from the state-of-the-art zebrafi sh facility and the access to marmosets.”

they recommended that future growth at ARMI proceed in a sustainable fashion, and suggested that targeted clinical links and recruitment be considered as the Institute grows.

the next retreat will be held in 2014.

ARMI Scientifi c Retreat

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ARMI is a founding participant and the headquarters for eMBl Australia, as well as the host for eMBl Australia’s first research node, the Victorian node.

eMBl Australia’s key focus to date has been to develop programs that support Australia’s young researchers and give them a flying start as they pursue careers in the life sciences. eMBl Australia offers talented young scientists up to nine years of secure funding in state-of-the-art facilities, giving them the opportunity to take some risks and really prove themselves. It’s a model that has proven successful at eMBl in europe in attracting and retaining high calibre scientists.

Dr nicolas Plachta and Dr edwina McGlinn, the two ARMI-based group leaders, are now entering their second and third years, respectively, at eMBl Australia and their hard work is starting to bear fruit in the form of publications and additional research grants. And in partnership with the South Australian Health and Medical Research Institute (SAHMRI), eMBl Australia is establishing a new node in Adelaide, funded by the three main South Australian universities and the State Government, to underpin their biomedical informatics capabilities.

At the other end of the career ladder, eMBl Australia is providing opportunities for PhD students to travel overseas to conferences and training courses at eMBl, exposing them to new ideas and technologies, and allowing them to begin building their own network of contacts, a valuable resource for their future.

eMBl Australia is also focused on building up Australia’s capabilities, through its unique collection of resources and alliances, including the Bioinformatics Resource Australia (BRAeMBl, formerly known as the eMBl Australia Bioinformatics Resource), located at the university of Queensland, and the Australian Bioinformatics network, which was formed in partnership with CSIRo and Bioplatforms Australia. In mid-2012, Graham Cameron, formerly head of eMBl’s european Bioinformatics Institute, joined BRAeMBl as its Director. through BRAeMBl and the Australian Bioinformatics network, we hope that the local bioinformatics community will flourish.

eMBl Australia’s newest initiative is SBI Australia, the first international node of Japan’s Systems Biology Institute. Both eMBl and eMBl Australia have had a long association with SBI Director, Professor Hiroaki Kitano, and we are looking forward to a long and fruitful association. through this initiative and others eMBl Australia is showcasing Australia’s life sciences research to the world.

about embL australiaeMBl Australia is a joint venture between the Group of eight universities and CSIRo, supported by the Australian Government’s science infrastructure investments.

eMBl Australia:• links Australian researchers to international

powerhouses of life science research• gives early-career researchers secure, long-

term research funding which enables them to take risks and ask big questions

• gives Australia’s best PhD students the chance to develop international networks and alliances and “calibrate” their work via eMBl’s european programs, workshops and conferences

• provides training programs for PhD students in Australia—giving them a head start in their science careers

• creates and shares life science resources with the Australian life science community.

embL (the european molecular biology Laboratory)eMBl is europe’s flagship for the life sciences. Its founders had a vision of a supranational research centre to redress the imbalance caused by uS domination of molecular biology.

eMBl was founded in 1974 and is funded by contributions from its 20 european member states. Australia is the first and the only Associate Member.

With nodes in Hinxton (near Cambridge, uK), Grenoble (France), Heidelberg and Hamburg (Germany), and Monterotondo (near Rome, Italy), eMBl comprises about 85 independent research groups and more than 1,400 people from 60 nations.

research

eMBl Australia

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Among its many features are:• nine years of funding security for research

leaders (subject to performance), after which they move on

• training for young researchers—over 3,000 per year

• highly sought postdoctoral positions • internationalising research networks across

europe and around the world• a culture that focuses on young scientists and

builds strong research alliances.

eMBl achieves goals beyond the reach of individual member states.

membership of embL Australia joined eMBl as an Associate Member in 2008. Australia’s Associate Member status runs until 2014.

Australia’s membership is managed by the Commonwealth Department of Industry, Innovation, Climate Change, Science, Research and tertiary education (now called the Department of Industry).

eMBl Australia maximises the benefi ts of Australia’s associate membership of eMBl via research support, infrastructure development and training opportunities across Australia.

In 2013, Australia’s status as an Associate Member of eMBl will be considered for renewal by both the Australian Government and eMBl’s executive Council.

embL australia’s core program in 2013eMBl Australia’s core program consists of the following research nodes, initiatives and activities:• Dr edwina McGlinn and her team, at ARMI, are

working to understand skeleton formation—how do cells in a limb bud know for example whether to form fi ngers or an upper arm bone?

• Dr nicolas Plachta and his team, also at ARMI, can track the movement of proteins as they turn genes on and off inside living cells—revealing how individual cells in an embryo change as they turn into specialised cells such as bone, nerves or skin.

• the nSW node at the university of Sydney comprises a single research group, headed by Dr Marcus Heisler. Marcus is currently based at eMBl in Heidelberg through the Faculty

Development Program, supported by the university of Sydney and the Australia Research Council, and is due to return to Australia in 2015 or 2016. there are plans for two further research groups.

• the South Australian node comprises three research groups being created at SAHMRI (the new South Australian Health and Medical Research Institute, which is a collaboration between Flinders university and the universities of Adelaide and South Australia) focusing on biomedical informatics. these three groups are expected to be established in late 2013–2014.

• A node will be developed at uWA as funds and opportunities arise.

• the Queensland node hosts the Bioinformatics Resource Australia (BRAeMBl) at the Institute of Molecular Biosciences at the university of Queensland. It provides access to international and Australian life science data enabling Australian researchers to access more data faster. In addition, a research group is likely to be co-located with the Bioinformatics Resource at the university of Queensland, subject to funding arrangements.

• the Australian Bioinformatics network is helping Australian scientists learn how to work smarter with bioinformatics, and helping Australian bioinformaticians share information and ideas. It supports a wide range of activities across Australia. It is based at CSIRo in Canberra and funded by eMBl Australia, CSIRo and Bioplatforms Australia.

• SBI Australia, an initiative based at Monash university, is introducing systems biology to Australian researchers via direct research, training and communication. It enables life scientists to harness computing technology and engineering methods to simulate complex life systems—the heart, the immune system, and whole organisms.

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2012 highlightsIn 2012 eMBl Australia:• developed and implemented a communications

strategy to spread the word about eMBl Australia, including monthly newsletters to interested stakeholders

• launched two student programs to send PhD students to eMBl for conferences and training programs

• launched the eMBl Australia alumni group for former eMBl and eMBl Australia students and staff

• established the Australian Bioinformatics network, as a joint program with CSIRo and Bioplatforms Australia

• appointed Dr David lovell as Director of the Australian Bioinformatics network

• appointed Mr Graham Cameron as the Director of Bioinformatics Resource Australia (BRAeMBl—formerly known as the eMBl Australia Mirror of the eMBl-eBI)

• appointed Professor Peter Currie as the Head of eMBl Australia’s Victorian node

• with funding from the university of new South Wales, sent Simone li to study at eMBl’s pre-doctoral program. She is the first Australian student to study at eMBl through the International PhD Program

• undertook a Mid-term Review of eMBl Australia and Australia’s status as an Associate Member of eMBl

• agreed to host the Australian node of Japan’s Systems Biology Institute, SBI Australia, at eMBl Australia’s Victorian node, based at ARMI

• established the South Australian node at SAHMRI and commenced recruitment for group leaders

• developed and launched the eMBl Australia PhD Course, a two-week residential program for first and second year PhD students.

2013 outlookDuring 2013 eMBl Australia will:• recruit up to three new group leaders for the

new node at SAHMRI• review and develop the eMBl Australia

governance model and develop guidelines for establishment of new groups and nodes

• establish a senior leadership team to oversee the further growth of eMBl Australia

• further develop the alumni group and hold events around Australia

• via the Bioinformatics Resource Australia, survey bioinformatics users around Australia to assess scientific needs and concerns

• review services and operations at BRAeMBl, and develop a clear strategy for the future of the Resource

• pursue relationships between BRAeMBl and related Australian projects to mutually enhance capabilities

• consolidate the role of the Australian Bioinformatics network in the Australian bioinformatics landscape

• increase the number of networking, training and education events through the Australian Bioinformatics network

• continue to progress the student grant programs to facilitate greater student exchange

• continue to develop and then run the first PhD Course in July 2013

• examine the viability of an eMBl Australia PhD program

• build on the current communications strategy and it expand to encompass all of eMBl Australia’s nodes and initiatives

• develop SBI Australia as a critical resource for eMBl Australia

• continue to lead planning and promotion for the 15th International Conference on Systems Biology (ICSB 2014), to be held in Melbourne from 13 to 19 September 2014.

research

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Japan’s renowned Systems Biology Institute (SBI) has established a node of the Institute in Australia, allowing it to expand into the Australian scientifi c landscape. the headquarters of the new node, known as SBI Australia, are hosted at ARMI.

SBI Australia is an initiative of eMBl Australia, in partnership with both ARMI and Monash university. It will connect Japanese and Australian research and industry partners, and facilitate the sharing of scientifi c technology, resources and expertise, in order to promote transnational systems biology research, and deliver tangible benefi ts to research and development in both countries.

At the centre of the initiative is the relationship between ARMI, eMBl Australia and the President of SBI, Professor Hiroaki Kitano. In March 2012, eMBl Australia and SBI signed a Memorandum of understanding to support Australian life science research through joint activities including collaboration and sharing of expertise via workshops, training and other opportunities that will enhance researchers’ understanding of the capabilities of genomics and bioinformatics.

the agreement to establish SBI Australia as the fi rst international node of the Systems Biology Institute was signed by Monash university Provost and Senior Vice-President, Professor edwina Cornish, in tokyo in october.

SBI Australia will develop and support Australian systems biology capacity through research, training and outreach, and will provide the platform to connect and promote Australian research to the international systems biology community.

the current direction of the SBI Australia research program includes:• characterising the systems that control the

development of the fi rst few cells in an embryo, with applications to improved IVF technologies

• understanding the different heart cell populations, especially non-myocyte cells (that is, cells other than muscle cells), to improve understanding of the normal and ageing adult heart

• investigating the robustness and fragility of coral reefs, which are affected by environmental perturbations, to develop countermeasures for coral bleaching that is a threat to the survival of coral reefs globally.

• facilitating access to Australia’s high-performance computing capacity, to support systems biology research through the provision of computing power

• facilitating access to software platforms and resources such as the Garuda Alliance, which is developing an open standard for sharing

systems biology data and information, and Flint, which provides the interface to high-performance computing resources.

SBI Australia also offers training to undergraduate and postgraduate students, and to postdoctoral researchers, in all relevant disciplines including biology, mathematics, computer science, physics, engineering, and chemistry.

2012 highlights• Dr Sarah Boyd was appointed Developer,

Systems Biology Research Platform, to lead the development of SBI Australia.

• SBI Australia was established at a signing ceremony on 17 october 2012 at the Ambassador’s Residence, Australian embassy, tokyo.

• the Australia-Japan Workshop on Biomedical Breakthroughs and Systems Biology was held on 16 october 2012 at the Japan Science and technology Agency tokyo Headquarters 2.

• An ARC Discovery Project Grant “Systems modelling of the cardiac fi broblast” was awarded ($270,000 over three years).

• the SBI Australia Steering Committee was established.

• Professor Hiroaki Kitano was appointed Sir louis Matheson Distinguished Visiting Professor.

• Dr Samik Ghosh was appointed an Adjunct Research Fellow.

• Dr Yukiko Matsuoka was appointed an Adjunct Research Associate.

systems biology conferenceIn late 2011, a consortium led by eMBl Australia successfully bid to host the 15th International Conference on Systems Biology (ICSB) in Melbourne from 13 to 19 September, 2014, at the Melbourne Convention and exhibition Centre.

eMBl Australia and SBI Australia will use the opportunity of hosting the conference to boost the profi le of systems biology in Australia.

Preparations for the conference got underway during 2012, with confi rmation of the dates and location as well as convening of the event management team to start developing the program.

the activities associated with eMBl Australia and SBI Australia place ARMI on a fi rm national and international footing, and support the thematic approach of the Institute to progress outstanding science and pursue national and international collaborations.

SBI Australia

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the rosenthaL group’s research concentrates on heart deveLopment, Immune and stem ceLL-drIven repaIr of neuromuscuLar and cardIac tIssue, and LImb regeneratIon, usIng the mouse and saLamander as modeLs for human response to damage and dIsease.

Rosenthal Group

research

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the Group is defi ning the factors that lead to a regenerative response and how these factors differ in a fi brotic response to injury, comparing the roles played by blood cell lineages in controlling infl ammation and promoting tissue repair in mammals and urodele amphibians. their research is exploring the exciting possibility of replacing damaged body parts through the new fi eld of regenerative medicine using gene therapy, cell transplantation and the induction of regeneration from the body’s own cells by altering the injury environment.

2012 highlights• Dr ekaterina Salimova joined the laboratory

as a Research Fellow.• Dr Alex Pinto published “An abundant tissue

macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profi le” in PloS one.

• nicholas lam published “nerve growth factor stimulates cardiac regeneration via cardiomyocyte proliferation in experimental heart failure” in PloS one.

group members group LeaderProfessor nadia Rosenthal

research fellowsDr Minna-liisa Anko (until May)Dr Mauro CostaDr Milena FurtadoDr James GodwinDr Alex PintoDr lina WangDr ekaterina Salimova

research assistantAnjana ChandranMr Drew Kuraitis (visiting from September)

phd studentnicholas lam

honours studentRyan Debuque

adjunctDr Minna-liisa Anko (from May)

collaborationsGrowth factors and cardiac stem cellsProfessor Richard Harvey, Victor Chang Cardiac Research Institute, Sydney, Australia

Stem cell delivery in cardiovascular diseaseDr Keith Mclean, CSIRo, Australia

Heart regeneration in zebrafi shProfessor David Kaye, Baker Heart Institute, Melbourne, Australia

Macrophages in lung diseaseProfessor Gary Anderson, university of Melbourne, Australia

IGF-1 in muscle regeneration Professor Miranda Grounds, university of Western Australia, Perth, Australia

Molecular mechanisms of cardiac recoveryProfessor Michael Schneider, Imperial College london, uK

Tissue factor and heart repairProfessor Dorian Haskard, Imperial College london, uK

Role of IGF- l in countering muscle diseaseAssociate Professor Antonio Musaro, university of Rome la Sapienza, Italy

Role of PWI in cardiac development and stem cell functionDr David Sassoon, université Paris VI/Pierre et Marie Curie, France

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research In the currIe Laboratory focuses on attempts to understand how the ceLLs of the vertebrate myotome are specIfIed to become IndIvIduaL muscLe ceLLs Later In deveLopment. we concentrate on two dIfferent popuLatIons of dIfferentIatIng muscLes, those that form the muscLes of the axIs and those that generate the muscLes of the fIns. we are Interested In the moLecuLar mechanIsms that make muscLes grow and the stem ceLL popuLatIons that are used to coordInate thIs process In normaL growth and regeneratIon. we are aLso Interested In how thIs process has evoLved throughout vertebrate specIes, and examIne the embryos of numerous LIvIng fIshes In an attempt to answer thIs questIon.

research

Currie Group

We are also are intrigued by zebrafi sh mutations that fail to undergo or retain the normal pattern of muscle differentiation within the embryo. We are particularly intrigued by mutations that mirror the onset of human muscular dystrophy and have developed zebrafi sh models of common muscular dystrophies. Muscular dystrophies are muscle-wasting diseases and among the most common genetic diseases of the newborn. they infl ict debilitating symptoms, are often fatal, and there are no effective cures.

the Currie Group has developed models of dystrophies in zebrafi sh and discovered that muscle cells detach during contraction. using the visually transparent nature of zebrafi sh in combination with nanofabrication, the Group is testing a new method to re-build damaged muscle attachment sites to allow muscles to survive. the Group was the fi rst to investigate zebrafi sh as a model for muscular dystrophy, a critical discovery for modelling human muscle pathology and among the fi rst reports of a zebrafi sh model of a human disease.

the Group provides resources and tools for the international zebrafi sh community and have developed novel imaging techniques, including optical projection tomography and X-ray tomography, to generate detailed 3D anatomical maps of zebrafi sh. the group has also developed an online anatomical resource for zebrafi sh—the fi rst embryo-to-adult atlas of any species in 3D.www.fi shnet.org.au

2012 highlights• Group members published 14 peer-reviewed

papers.• Group members were awarded two nHMRC

grants, “Modelling laminin mediated adhesion and congenital muscular dystrophy in zebrafi sh” and “Molecular mechanisms that generate and activate muscle stem cells during growth and disease”.

• Group members were awarded an ARC grant.• Group members received an ARC linkage

Grant “Generation of a mutational resource in Zebrafi sh”.

• Dr Patricia Jusuf was awarded a prestigious ARC DeCRA Fellowship “Role of intrinsic versus extrinsic cues in cell type determination during development and regeneration”.

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research publication highlights 2012Goldshmit Y, Sztal TE, Jusuf PR, Hall TE, Nguyen-Chi M, Currie PD. Fgf-dependent glial cell bridges facilitate spinal cord regeneration in zebrafi sh. J neurosci. 2012 May 30;32(22):7477-92.

understanding how the spinal cord of zebrafi sh can regenerate so effectively was a new research direction for the Group in 2012. A major goal of this research direction is to understand the signals and processes that zebrafi sh use to undergo regeneration and why they differ to the non-regenerative context of mammalian spinal cord injury. Amazingly, after a full spinal cord resection zebrafi sh can fully regenerate nerves and their connections in the spinal cord to generate full locomotor recovery. this exciting paper describes the molecular signal and cellular behaviours that zebrafi sh use to regenerate their spinal cord.

Nguyen-Chi ME, Bryson-Richardson R, Sonntag C, Hall TE, Gibson A, Sztal T, Chua W, Schilling tF, Currie PD. Morphogenesis and cell fate determination within the adaxial cell equivalence group of the zebrafi sh myotome. PloS Genet. 2012;8(10):e1003014.

one of the core interests of the Currie Group is to understand how muscle cells arise in the early embryo. this paper reveals that an intruiging interplay of signals and cell movements generate the early muscle of the zebrafi sh embryo that gives a window on how different cells are programmed to make different muscle types.

Sztal TE, Sonntag C, Hall TE, Currie PD. epistatic dissection of laminin-receptor interactions in dystrophic zebrafi sh muscle. Hum Mol Genet. 2012 nov 1;21(21):4718-31.

one of the focuses of the Currie Group is to use the zebrafi sh model to understand how the loss of certain genes leads to the onset of muscular dystrophy. this paper examines zebrafi sh models of two of the most common forms of human muscular dystrophy, Duchenne muscular dystrophy and congenital muscular dystrophy, and examine how the genes mutated in these disorders coordinate muscle cell integrity in living muscle tissue.

group membersgroup LeaderProfessor Peter Currie, Deputy Director, ARMI

research fellowsDr Joachim BergerDr Catherine BoisvertDr thomas Hall (until January)Dr Patricia Jusuf Dr Yona GoldshmitDr Ivana Mirkovic (from September)Dr Ashley Siegel

research assistants Ms Carmen Sontag Ms Silke BergerMs Fruszina Fenyes (from August)Ms lei Mei

phd studentsMs ophelia erhlich Mr David GurevichMr Wouter Masselink Mr Phong nguyen Mr Zhenhua li

collaborationsNano particle and tube for in vivo ECM assembly in zebrafi sh Dr Patrick Hartley, Dr Richard Williams, Dr Keith Mclean, CSIRo, Australia

Muscle attachment in the zebrafi sh embryoProfessor tom Schilling, university of California, Irvine, uSA

Testing muscle disease models in zebrafi shProfessor Christina Mitchell, Monash university, Melbourne, Australia

Mutants affecting muscle formation in zebrafi shProfessor Didier Stainier, university of California, San Francisco, uSA

The role of Lbxl in developmentDr Christine thisse, Institute de Génétique et de Biologie Moléculaire, Strasbourg, France

Generating a mutation resource in zebrafi shProfessor Graham lieschke, ARMI

Heart regeneration in zebrafi shProfessor nadia Rosenthal, ARMI

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stem ceLL bIoLogy offers great promIse for fIndIng new treatments for degeneratIve dIseases and cancer. stem ceLL dIfferentIatIon studIes are aLso heLpIng to reveaL ceLLuLar and moLecuLar mechanIsms governIng ceLL fate and tIssue specIfIcatIon.

Barberi Group

research

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the Barberi Group is researching methods to isolate specifi c stem/precursor cells originating from human embryonic stem cells (heSC) that may have therapeutic applications. using heSC as a research tool, the Group also aims to address fundamental questions in development, such as how pluripotent cells undergo lineage restriction and fate specifi cation. In particular, the Group is interested in the development and differentiation of striated skeletal muscle cells, the development and differentiation of the retina, and neural crest and cranial placode specifi cation during early neurulation.

the Group is also studying neural development using mutant mouse eSC lines. they are examining how misexpression of Wnt-l could affect the neural differentiation potential of eSCs, and analysing the role of the transcription factor SCl/tall during neural development using specifi c gain of-function and lack-of-function SCl/tall mutant eSC lines.

group membersgroup LeaderAssociate Professor tiziano Barberi

research fellowsDr Isabella Mengarelli (until September)Dr Joly Kwek

research assistantMr Joseph Chen (from March)

phd studentMs Bianca Borchin

collaborationsDr Jose Polo, Monash Immunology and Stem Cell laboratories, Monash university, Melbourne, Australia

Professor Giovanni Morrone, university of Catanzaro, Italy

Professor Jonathon Crowston, Centre for eye Research Australia (CeRA), Melbourne, Australia

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damage or trauma to the braIn has profound Impact on IndIvIduaLs, theIr famILIes and the heaLth care system. these types of InjurIes occur as a resuLt of, for exampLe, a car accIdent or stroke. no current treatments wILL effectIveLy repaIr or promote the regrowth of damaged braIn tIssue.

Bourne Group

research

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the Bourne Group is addressing the molecular processes of degeneration and regeneration of cells in a region of the brain known as the neocortex of the primate. examining how this region responds at different ages is a key step to understanding why the young brain has a remarkable capacity to repair itself following an injury, while the adult brain does not. the Bourne Group is trying to elucidate the key mechanisms of repair in the infant brain in order to attempt to recapitulate the process in the adult following an injury.

the Group is tackling these questions from a cell-to-system approach, looking at how cells in culture respond to certain molecules through to observing physiological and anatomical changes in the animal model.

2012 highlights• Group leader James Bourne was promoted to

Associate Professor.• Associate Professor James Bourne was

awarded an nHMRC Project Grant for “A role for the pulvinar nucleus in visual cortical development and plasticity”.

• Group members published six papers in internationally renowned journals.

• Group members gave presentations at leading overseas meetings.

• the fi rst demonstration of the existence of neural precursor cells in the visual cortex of the non-human primate.

• Faculty of 1000 recommended the journal article: Warner, C.E., W.C. Kwan, and J.A. Bourne, the early Maturation of Visual Cortical Area Mt is Dependent on Input from the Retinorecipient Medial Portion of the Inferior Pulvinar. J neurosci, 2012. 32(48)17073-17085. this work demonstrated the importance of the pulvinar nucleus in the development of the visual cortex.

• Associate Professor James Bourne participated on the nHMRC Project Grant Review Panel.

group membersgroup LeaderAssociate Professor James Bourne

research fellowDr Jihane Homman-ludiye

research assistantMr William Kwan

phd studentsMr leon teoMs Claire Warner

affi liatesDr tobias MersonDr Julie thacker (from June)Professor Anita Hendrickson

honours/urop studentsMs Skye KinderMr Anthony Boghdadi

collaborationsStem cells and repair in the injured nonhuman primate visual cortexDr toby Merson, Professor trevor Kilpatrick, Florey neurosciences Institute, Melbourne, Australia

The role of Eph/ephrins in brain development and repairDr Jennifer Rodger, School of Zoology, university of Western Australia, Perth, Australia

Developmental plasticity in the nonhuman primate visual cortexDr Samuel Solomon, Department of Physiology, university of Sydney, Australia

Novel conjugated biomaterials in the treatment of neurotrauma and neurodegenerationProfessor Jeffrey Rosenfeld, Alfred Hospital, Melbourne; Dr Pat Hartley, Dr Keith Mclean, Biomedical Materials, CSIRo, Australia

Hydrogels for the treatment of brain injuryDr John Forsythe, Dr David nisbet, Department of Materials engineering, Monash university, Melbourne, Australia

Distribution of molecular guidance molecules in the primary sensory fi elds of the developing marmoset monkeyDr Zoltan Molnar, Department of Physiology, Anatomy and Genetics, university of oxford, uK

Diffusion imaging of the nonhuman primate visual system following lesions of the neocortexProfessor Gary egan, Dr leigh Johnston, Dr Scott Kolbe, Florey neuroscience Institute, Melbourne, Australia

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the heng group joIned armI In june 2010 wIth the appoIntment of dr juLIan heng foLLowed by members of hIs team. the heng group InvestIgates the moLecuLar and ceLLuLar mechanIsms that controL nerve ceLL productIon and maturatIon wIthIn the mammaLIan braIn. the group uses moLecuLar technIques to study the bIrth and deveLopment of cerebraL cortIcaL neurons wIthIn the foetaL mouse braIn In vIvo as weLL as In vItro. these studIes are combIned wIth bIoInformatIcs approaches to IdentIfy, then characterIse, genes responsIbLe for aspects of the maturatIon of newborn nerve ceLLs. uLtImateLy, the goaL of our research Is to use our fIndIngs to dIrect the productIon of specIfIc neuraL ceLL types from donor stem/progenItor ceLLs, and to deveLop these as noveL substrates for braIn transpLantatIon and repaIr In braIn Injury or dIsease.

Heng Group

research

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In a parallel approach, the Heng Group investigates the genetic basis for structural brain disorders (such as microcephaly) in humans. these studies have led to the identifi cation of novel genes which, when mutated, cause brain developmental defects. one important goal from this work is to identify novel genetic markers for diagnostic testing of patients with brain developmental disorder. In the future, these studies could eventually lead to the development of new treatments, gene therapies or patient-specifi c clinical interventions for the treatment of their neurodevelopmental disorders.

2012 highlights• Group members were awarded an ARC

Discovery Project Grant “Subcellular recruitment of a RhoA ubiquitination complex by Rnd proteins”.

group membersgroup LeaderDr Julian Heng

research fellowsDr Matilda HaasDr lieven Huang

research assistantsMr Zhengdong QuMs Shanshan liMs linh ngo

phd studentMr Ivan ng

honours studentMs Stephanie Houston

collaborationsTranscriptional control of neural developmentDr Haruo okado, Dr Chiaki ohtaka-Maruyama, tokyo Metropolitan Institute for neuroscience, Japan; Professor Masataka Kasai, national Institute of Infectious Diseases, Japan; Dr Francois Guillemot, the national Institute for Medical Research, uK

The genetics of mammalian brain developmentProfessor Seong-Seng tan, the Florey Institute of neuroscience and Mental Health, Australia

The effects of prenatal drug exposure in brain patterning and functionDr Christopher Reid, the Florey Institute of neuroscience and Mental Health, Australia

The genetics of brain developmental disorders in humansDr David Keays, Institute of Molecular Pathology, Austria

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dr robIn hobbs reLocated from beth IsraeL deaconess medIcaL center assocIated wIth harvard medIcaL schooL In boston In earLy 2012 and has a joInt appoIntment wIth the department of anatomy and deveLopmentaL bIoLogy at monash unIversIty.

Hobbs Group

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the main focus of the Hobbs Group is to defi ne critical mechanisms underlying adult stem cell function through use of germline stem cells from the mouse testis as a model. Maintenance of a wide array of adult tissues is dependent on a resident population of stem cells that must self-renew and generate differentiating daughter cells. the appropriate control of stem cell self-renewal and differentiation is critical for tissue homeostasis while disruption of the balance between these processes can contribute to tissue degeneration or cancer.

the adult testis contains a population of germline stem/progenitor cells (known as spermatogonial progenitor cells or SPCs) that are required for life-long production of differentiating germ cells and spermatozoa. A handful of cell intrinsic factors are known to be involved in SPC maintenance, foremost among which is the transcription factor Promyelocytic leukaemia Zinc Finger (PlZF).

A major focus of the Hobbs Group is to defi ne downstream targets of PlZF in SPCs and their role in SPC function. this is achieved by using a combination of mouse genetics, fl ow cytometry analysis and in vitro culture techniques. the principal aim of this research is to identify and characterise molecular mechanisms underlying adult stem cell function. this work has particular relevance for our understanding of male infertility and can suggest novel therapeutic approaches aimed at improving adult stem cell function and tissue repair following injury.

2012 highlights• Dr Robin Hobbs was awarded a Monash

university larkins Fellowship to establish his laboratory at ARMI.

• Dr Hobbs started as a group leader at ARMI in February, having relocated from the Beth Israel Deaconess Medical Center, associated with Harvard Medical School, uSA.

• Dr Hobbs published a high-profi le fi rst author paper in the Cell Stem Cell journal and a co-second author paper in Cell.

• Dr Hobbs was invited to present his work at the annual conference of the Society for Reproductive Biology and endocrine Society of Australia.

• Dr Hobbs was awarded an nHMRC Project grant “Role of PlZF – SAll4 interactions in germline progenitor function and development”.

group membersgroup LeaderDr Robin Hobbs

research assistantMs Mia de Seram (from June)

collaborationsRole of the GILZ gene in male germline stem cell maintenance and fertilityProfessor eric Morand, Department of Medicine, Centre for Infl ammatory Diseases, Monash Medical Centre, Monash university, Melbourne, Australia

Characterising the role of Activin signalling in postnatal testis developmentProfessor Kate loveland, Department of Biochemistry & Molecular Biology, Monash university, Melbourne, Australia

Generation and characterisation of germline stem cell-derived pluripotent stem cellsProfessor Fiorella Altruda, Molecular Biotechnology Center, university of turin, Italy

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the kasLIn group focuses on understandIng the moLecuLar and ceLLuLar mechanIsms that controL ceLLuLar pLastIcIty In the Intact and Injured vertebrate braIn.

Kaslin Group

research

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neural stem cells and brain regeneration have been mostly studied in vertebrates (rodents) that have very limited neurogenic potential. In contrast, we have found that zebrafi sh exhibit widespread adult neurogenesis and are able to regenerate parts of their central nervous system. these features make it possible to tackle questions in the zebrafi sh that are not possible in mammalian models. our overall aim is to understand the underlying molecular and cellular mechanisms that allow/limit cellular plasticity in the vertebrate brain.

the group uses high-resolution in vivo imaging, novel genetic tools and cellular reprogramming to study how neuronal stem cell niches and tissue permissiveness arise and are being maintained. using high-throughput sequencing we want to get a comprehensive understanding of the genetic networks that regulate cellular plasticity during homeostasis and regeneration. the elucidation of the mechanisms that govern cellular plasticity in vivo is essential for successful central nervous system therapies in the future.

group membersgroup LeaderDr Jan Kaslin

research assistantsMs Krutika Wikhe (until June)Ms Jean tangMr Raphael Park ChaeMr Sunil Kumar (from August)

phd studentMs Frisca Frisca

urop studentsMr Daniel Colquhoun Ms Celia Vandestadt

collaborationsDevelopment & regeneration of hypothalamic circuits Dr Gil lewkowitz, Weizmann Institute, Israel

Wnt signalling & regenerationDr Gilbert Weidinger, university of ulm, Germany

Genome-wide analysis of brain regeneration in zebrafi sh Dr Michael Brand, Center of Regenerative therapies Dresden, Germany

Tools for studying signalling pathway activity in zebrafi sh Dr Francesco Argenton, university of Padua, Italy

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the LIeschke group joIned armI In november 2010, transferrIng from the cancer and haematoLogy dIvIsIon of the waLter and eLIZa haLL InstItute of medIcaL research, meLbourne. the group studIes bLood deveLopment and functIon usIng ZebrafIsh and was one of the fIrst groups In the worLd to use the genetIc fLexIbILIty and LIve ImagIng capacIty of ZebrafIsh for whIte bLood ceLL research. the group has aLso deveLoped an InfectIon modeL to InvestIgate the roLe of whIte bLood ceLLs (Leukocytes) In the host-pathogen response.

lieschke Group

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the haemopoietic system makes blood cells and is a prolifi cally regenerative tissue, constantly supplying blood cells throughout life. It is capable of large bursts of replenishment following injuries and interventions such as chemotherapy. leukocytes are key cellular players in host defence and as effectors of infl ammation. their function plays a major role in determining the balance between ongoing tissue injury, scarring, healing and regeneration.

Professor lieschke is internationally recognised for his research into blood disorders and cancer using zebrafi sh and mice. He is also a clinical haematologist at the Royal Melbourne Hospital, where he treats patients with leukaemia and lymphoma.

2012 highlights• Group members identifi ed a way that white

blood cells turn off the signal that fi rst attracts them to a site of infl ammation, published in Current Biology.

• Many researchers around the world are using transgenic macrophage reporter zebrafi sh lines recently developed in the lab.

group membersgroup LeaderProfessor Graham lieschke

research fellowsDr Cristina KeightleyDr Felix ellettDr Chieh-Huei Wang (from october)

senior research assistantMs Sony Varma

phd studentsMr Sultan Alasmari Mr Vahid Pazhakh

urop studentMs emily Scott

visiting studentMr Johannes Wittig

collaborationsAn in vivo model of infection with the opportunistic human pathogen Penicillium marneffeiAssociate Professor Alex Andrianopoulos, Department of Genetics, university of Melbourne, Australia

Characterisation of a novel BTB-ZF transcription factorProfessor Stephen Jane, Bone Marrow Research laboratories, Royal Melbourne Hospital, Melbourne, Australia

Live imaging and quantifi cation of leukocyte migration in vivoDr Stephen Renshaw, university of Sheffi eld, uK

Cloning and characterisation of zebrafi sh mutants with defects of intestinal developmentAssociate Professor Joan Heath, ludwig Institute for Cancer Research, Melbourne, Australia

Tools for studying leukocyte behaviours in zebrafi shProfessor Phil Crosier, university of Auckland, new Zealand

Building an Australian zebrafi sh TILLING libraryAssociate Professor Joan Heath, ludwig Institute for Cancer Research, Melbourne, Australia; Professor Peter Currie, ARMI

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the marceLLe group Is Interested In understandIng how functIonaL skeLetaL muscLe arIses from a group of unspecIaLIsed mesodermaL ceLLs. to achIeve thIs, crucIaL ceLL fate decIsIons, extensIve mIgratIon, tIghtLy reguLated ceLL dIvIsIons take pLace In just a few days of embryonIc deveLopment. we want to understand the ceLLuLar and moLecuLar processes that underLIe thIs compLex process.

Marcelle Group

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the animal models on which we perform our experiments are the mouse and chick embryos. the fi rst aim of our research is to observe the complex cellular events that take place during muscle formation, using cutting-edge in vivo imaging technologies. the second aim is to understand the molecular mechanisms underlying muscle fusion. Finally, we want to identify gene networks implicated in the maintenance and differentiation of muscle stem cells.

Professor Christophe Marcelle is a world leader in the application of developmental biology to the study of muscle growth and regeneration. His work has illuminated key aspects of how muscle precursor cells form in the early embryo and how they contribute to muscle growth throughout life. Christophe’s work at ARMI continues his focus on muscle development and stem cell biology, with the ultimate aim being to understand how the muscle organ system forms. Before joining ARMI, Christophe led a research group at the Developmental Biology Institute of Marseille-luminy (IBDMl) at the university of Marseille in France.

research themesmuscle stem cellsWe utilise an integrated systemic approach to defi ne a core gene network governing “stem-cellness” in muscle. A fi rst part of the project involves the generation of new mouse lines. Isolated stem cells from these lines are then used to perform microarray, and ChIP-seq analyses. Functional analyses are performed in the chick embryo.

myoblast fusionSkeletal muscle-fi bres are plurinucleated. this occurs through multiple rounds of myoblast fusion, taking place during development and during muscle regeneration and repair in the adult. A whole genome screen has been made to identify putative candidate genes important in fusion. We are now performing functional analyses in the chick and mouse embryos.

morphogenesis of skeletal musclesMuscle tissue is extremely well organised, fi bres are aligned in a precise direction, and their attachment points are well defi ned. How this process is regulated at a cellular and molecular level is unknown. High-end imaging technologies are used to address this question in live chick embryos.

2012 highlights• Cyril Picard published his PhD thesis. • Group members published six papers.• Group members were awarded two nHMRC

grants.• Professor Christophe Marcelle attended four

international conferences and was keynote speaker at the Israel Society for Development Biology Conference.

group membersgroup LeaderProfessor Christophe Marcelle

research fellowsDr olivier SerralboDr Claire Hirst (from September)Dr Frederico Calhabeu (from May)Dr Ashvind Prahahran (until September)

research assistantsMr Cyril Picard (until December)Ms nadege Veron

phd studentMr Daniel Sieiro Mosti

honours studentJeremy ng Chi Kei

urop studentMr Mark Sedrak

adjunct/affi liatesDr David SalgadoDr Manuel Pele

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the mcgLInn group uses the deveLopIng LImb bud and axIaL skeLeton to understand genetIc hIerarchIes governIng patternIng mechanIsms.

McGlinn Group

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the reiterative use of key signalling pathways across multiple organs has meant that the limb, with its advantages of ease of manipulation and lack of requirement for embryonic survival, has provided fundamental contributions to our understanding of broader developmental processes.

More recently, it has become clear that a comprehensive understanding of these processes requires integration of all levels of gene regulation, including both protein-coding and non-protein-coding mechanisms.

For this reason, we have pioneered a novel approach to investigate the role of microRnAs in development.

We aim to build a more complete molecular roadmap of how the size, shape and number of bones form within the early vertebrate embryo.

2012 highlights• Dr Jesus Casanova joined the McGlinn Group in

July 2012. Jesus received his PhD at the CnIC, Madrid, Spain, and is interested in the regulation of vertebral number by microRnAs.

• Dr edwina McGlinn was awarded a nHMRC project grant “elucidating the role of miR-196 in formation of the axial skeleton”.

• Group members published one research article in the high impact journal Genes and Development and two review articles.

group membersgroup LeaderDr edwina McGlinn

research fellowDr Alysha Heimberg Dr Jesus Casanova (from July)

research assistantMs lisa Wong

phd studentMr eamon Coughlin

affi liateMr Janus Jacobsen (from January to July)

collaborationsA role for miR-196 in patterning the vertebrate embryoProfessor Clifford tabin, Harvard Medical School, uSA; Professor David Bartel, Whitehead Institute, MIt, uSA

The microRNA repertoire of the King Cobra, Ophiophagus hannahProfessor Michael Richardson, leiden university, the netherlands

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Plachta Group

reveaLIng the dynamIc mechanIsms that pattern a mammaLIan embryo Is key to understandIng human bIoLogy and dIsease, yet few experImentaL systems permIt the study of dynamIc physIcaL aspects of ceLLs and moLecuLes In LIvIng mammaLIan embryos.

research

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the Plachta Group combines single-cell imaging and quantitative methods to discover how the dynamic behaviour of DnA-binding molecules controls the development of the fi rst specialised cells in living mouse embryos. the Group recently established new experimental assays to visualise the movement of transcription factors, which are key regulatory molecules controlling gene expression, in four dimensions (x, y, z and time).

the Group undertakes in imaging in real time at the single-cell level in intact embryos. this approach allows them to probe biochemical events typically studied in fi xed specimens or in cell homogenates often lacking the spatiotemporal dynamics of in vivo systems.

Group members extend these studies by comparing pluripotent cells in the embryo to several stem cell lines cultured in vitro, which are derived from the actual embryo (such as embryonic stem cells) or that are reprogrammed from somatic cell lineages (induced pluripotent stem cells). In addition, the Group has developed live imaging tools to study the cellular mechanisms governing the formation of the fi rst tissue-like structures in the embryo, with a particular focus on cell movements and formation of the central nervous system.

2012 highlights• Dr nicolas Plachta was awarded an nHMRC

grant “Revealing how transcription factors search the DnA to control preimplantation development in mammals”.

group membersgroup LeaderDr nicolas Plachta

research fellowsDr Gurpreet Kaur Dr Juan Carlos Fierro-Gonzalez (from March)Dr Melanie White (from May)Dr Jennifer Zenker (from December)

research assistantMr Juan Silva

affi liateMr tim Hast (from September)

collaborationsImaging the dynamics of cell reprogrammingDr Jose Polo, MISCl, Monash university, Melbourne, Australia

Analysing gastrulation in mammalsDr Samuel ojosnegros, California Institute of technology, uSA

Designing new tools for single-cell imagingDr Sarah Boyd, School of Mathematics, Monash university, Australia

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Polo Group

the group Is Interested In the transcrIptIonaL and epIgenetIc mechanIsms that govern ceLL IdentIty, In partIcuLar pLurIpotency and the reprogrammIng of somatIc ceLLs Into Induced pLurIpotent stem (Ips) ceLLs.

research

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Being able to reprogram any specifi c mature cellular program into a pluripotent state, and from there back into any other particular cellular program, provides a unique tool to dissect the molecular and cellular events that permit the conversion of one cell type to another. Moreover, iPS cells and the reprogramming technology are of great interest in pharmaceutical and clinical settings. the technology can be used to generate animal and cellular models for the study of various diseases and may in the future provide cells tailor-made for a specifi c patient to be used in cellular replacement therapies. However, despite being one of the major growing research fi elds, very little is known about the epigenetic and transcriptional changes occurring during this process. understanding the events leading to the generation of iPS cells is a necessary step to ultimately using iPS cell technology for therapeutic purposes. By using a broad array of approaches through the use of mouse models and a combination of different molecular, biochemical, cellular techniques and genome wide approaches, our lab aims to dissect the nature and dynamics of such events.

We are particularly interested in the following aspects:• the kinetics and universality of the epigenetic

and genomic changes occurring during reprogramming

• the composition and assembly kinetics of transcriptional regulation complexes at pluripotency genes

• how the cell of origin infl uences the in vitro and in vivo plasticity potential of cells generated during the reprogramming process

• the epigenetic changes occurring in adult stem cells as a consequence of changes in their environment.

2012 highlights• Dr Jose Polo became a nHMRC Career

Development Fellow in January 2012.• Dr Polo was awarded two nHMRC project

grants to start in 2013.• the Group published a paper in the journal

Cell: Polo, J.M., e. Anderssen, R.M. Walsh, B.A. Schwarz, C.M. Nefzger, S.M. Lim, M. Borkent, e. Apostolou, S. Alaei, J. Cloutier, o. Bar-nur, S. Cheloufi , M. Stadtfeld, M.e. Figueroa, D. Robinton, S. natesan, A. Melnick, J. Zhu, S. Ramaswamy, and K. Hochedlinger, “A Molecular Roadmap of Reprogramming Somatic Cells into iPS Cells”.

• Dr Polo published a paper in Cell Reports.

group membersDr Susie nilsson, CSIRoDr Stefan White, Monash Institute of Medical ResearchProfessor ed Stanley, Monash universityProfessor Andrew elefanty, Monash universityDr Helen Abud, Monash universityDr nicolas Plachta, ARMIAssociate Professor tiziano Barberi, ARMI

collaborationsgroup LeaderDr Jose Polo

research fellowsDr Sue Mei limDr Christian nefzger

phd studentSara Alaei

undergraduate studentJaber Firas

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core research facILItIes

hIgh-end core research facILItIes are IntegraL to the armI research program and to enabLIng our researchers to maxImIse theIr research output and quaLIty. aLong wIth monash unIversIty and wIth the support from the vIctorIan and austraLIan governments, the InstItute has Invested sIgnIfIcantLy In these very Important assets.

“

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FishCore is the largest zebrafi sh facility of its kind in the southern hemisphere. It includes 1,000 quarantine and 5,200 non-quarantine tanks.

In 2010, the FishCore team grew the number of zebrafi sh strains to 29. the facility is now operating at 85% capacity, servicing ARMI research groups and an external research team. through an alliance with Box Hill tAFe, animal husbandry students have gained work experience at FishCore. two students from this program have gone on to be engaged as casual employees. FishCore is undergoing an expansion to also house axolotls and sharks. Design of the facility is underway, with construction expected to commence in 2013.

As part of this expansion, the team, in collaboration with ARMI researchers, has established pilot axolotl and shark facilities. using these facilities, research programs using these models have been established in the lead-up to the construction of the AquaCore facility.

facilities• PC2 zebrafi sh housing facilities• PC2 research microinjection facilities• AQIS-approved oAP zebrafi sh quarantine facility• Recirculation and fi ltration systems to ensure

optimal environmental conditions• Specialised aquatic animal husbandry staff

capabilities and services• training in the use of zebrafi sh as a research

model• Purchasing and agistment of zebrafi sh strains• Zebrafi sh wild type, transgenic, and mutant

embryo production• International and interstate importation and

AQIS-approved quarantining of zebrafi sh strains• exportation of mutant and transgenic strains of

zebrafi sh• Cryogenic freezing and rederivation of zebrafi sh

strains

stafffacility managerMr Julian Cocks

techniciansMr eren YilgorMr Rodney GlanvillMr Pitra YudhyantaraMr Maik FiedelMs emma CarlosMs Jamie MihelakosMs olivia FerraroMs tanya HynesMs natalie Bien-IzowskiMs natalie Wong

www.monash.edu/research/infrastructure/platforms/fi shcore.html

FishCore

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the ARMI Gene Recombineering Facility provides specialised modifi cation of genomic material from multiple organisms. Most constructs made are targeting constructs and transgenic constructs.

Homologous recombination is used for construct assembly. this enables the position of a DnA construct to be altered, without having to rely on restriction endonucleases.

the ARMI Gene Recombineering group works closely with eS Cell Services and Monash Animal Research Platform to offer a comprehensive design and screening service.

capabilities and services• Construct design• Southern screening strategy design (including

design of the southern probes)• Shaving of BAC clones• Subcloning pieces of BAC clones into high copy

plasmids• BAC constructs (FlP lines, Cre lines, reporter

lines)• Conditional knock out constructs• Knock in constructs• Promoter fusion constructs• Introduction of point mutations• Humanising animal models• Construct cloning

staffdirectorMs Jeanette Rientjes

research assistantsMs Margareta GoMs Susan thomas

Gene Recombineering


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the ARMI Mouse embryonic Stem Cell Core produces mouse eS cells with specifi c gene modifi cations. the facility offers a range of services, from electroporation of DnA constructs into eS cells, to the identifi cation of correctly targeted clones.

Working closely with Monash Gene targeting Facility (MGtF), the facility prepares eS cells for microinjection and organises screening of the chimeric mice generated, as well as analysis of germ line transmission.

the facility collaborates with the Australian Phenomics network in the production of chimeras from eS cells obtained from the International Knockout Mouse Consortium (IKMC).

capabilities and services• electroporation of DnA constructs into eS cells• Screening the selected clones by Southern blot• thawing and expanding of the positive clones• Re-confi rming by Southern blot the positive

clone and sequencing of important sites of the mutated locus, such as loxPs, FRts and eventual mutation knocked-in to the gene

• Preparation of eS cells for microinjection• Screening of chimeric mice and germ line

transmission by PCR analysis

staffmanagerDr Arianna nenci

research fellowsDr Melissa CooneyDr Dirk truman

technical staffMs Wendy Chua

eS Cell Genetics Services

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Researchers at the university of Southern California (uSC) recently demonstrated the only proven and authentic germline competent rat eS cell enabling technology that can use rats for human disease modelling via precise genetic engineering [Cell 2008: 135,1287; nature 2010: 467, 211]. ARMIRat is an academic collaboration linking the uSC technology with advanced conditional mutagenesis and animal modelling at ARMI. It combines expertise in rat animal husbandry and embryo manipulation (Monash transgenic Rodent Services), molecular biology and genetic engineering (ARMI Gene Recombineering Core), eS cell technology and expertise and broad experience in derivation, maintenance and manipulation of mammalian embryonic and tissue-specific stem cells, and their differentiated derivatives (ARMI and uSC).

the outcome will be a platform with expertise and capacity to provide transgenic rat services to the life sciences community. It will be operated as a service provided by ARMI on a fee-for-service basis. the last two decades of life science has witnessed a technological explosion in mouse genetics and genomics, which promises to yield a wealth of information on mammalian gene function in ageing and disease. However, this has shifted attention away from other valuable mammalian models such as the rat. the larger size and metabolic similarity to humans have long made rats a preferred reference animal for surgical interventions, physiological research and pharmaceutical development. the initial rat models generated will focus on two clinically relevant models: hypertension and cardiac disease. once the technology is established, collaboration with other groups will lead to new rat models to study other diseases (for example, for diabetes and atherosclerosis). the ARMIRat project commenced in 2010 with technical expertise provided by Jeanette Rientjes (Gene Recombineering), Dr Arianna nenci (eS Cell Services) and Dr Jose Gonzalez (MGtF). Dr nenci and Dr Gonzalez trained with the uSC team, Dr Qi-long Ying and Dr Chang tong, on techniques to be adopted in the transgenic Rat Platform.

Support for the ARMIRat Project is provided by the Victorian Department of Business and Innovation and the Australian Department of Health and Ageing.

ARMIRat


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FlowCore is a purpose-built core facility providing fl ow cytometry services to researchers from ARMI, Monash university, the Australian Stem Cell Centre, and the wider scientifi c community.

Flow cytometry is a high-speed laser-based technology for analysing the characteristics of cells, organisms and particles. Researchers tag their samples with fl uorescent probes, dyes or proteins before running the samples through multiple laser beams, exciting these probes. the cytometer measures the light scatter and fl uorescence of the cells or particles, rapidly generating multi-parametric data about the size, complexity, protein expression and cellular processes within the sample, on a cell-by-cell basis. Samples can be sorted into sub-populations based on these parameters, allowing researchers to conduct experiments on pure populations of cells or particles.

Flowcore is a PC2 certifi ed facility run by four full-time staff who provide quality controlled fl ow cytometry experiments and expert advice, allowing for better reproducibility of results and high quality research. FlowCore also houses Australia’s fi rst fi ve-laser cell sorter and seven-laser analyser, allowing greater fl exibility for researchers.

facilities• 5 laser BD Infl ux cell sorter• 4 laser BD Infl ux cell sorter• 3 laser BD FACSVantage DiVA cell sorter• 4 laser BD lSRII analyser• 7 laser BD lSRII analyser

capabilities and services• High-speed cell sorting• High-throughput fl ow cytometric analysis• training and education• Data analysis software

www.monash.edu/research/infrastructure/platforms/fl owcore.html

FlowCore

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the Micromon High next-Generation Sequencing (nGS) Facility is the second longest running Illumina nGS facility in Australia, offering sequencing, bioinformatics, quantitative real-time PCR, acoustic shearing and nucleic acid quantitation services. utilising Illumina Genome Analyzer GAllx, HiSeq and MiSeq sequencing instruments, the facility can produce sequencing data at up to approximately 16Gb per day. Micromon can carry out a diverse range of applications aside from standard DnA sequencing, such as transcriptomics (RnA sequencing) for both prokaryotic and eukaryotic organisms, small RnA sequencing and discovery, DnA-protein interaction studies, DnA methylation analysis, custom amplicon sequencing and custom exome capture. In addition to our sequencing capacity, we also provide a nucleic acid, cell and tissue fragmentation service based on the closed-vessel Covaris focused acoustic shearing. our nucleic acid quantitation service is based on the the Agilent Bioanalyzer 2100 and Invitrogen Qubit system. Micromon services the research needs of local, national and international researchers.

In 2012 the team introduced new sequencing instrumentation, capabilities and services based on the Illumina HiSeq and MiSeq technology platforms. this will improve sample throughput, decrease sequencing costs, improve sample turn-around time and increase data quality. our quantitative real-time PCR service also commenced in 2012 and this operates as a stand-alone service and also as a complementary service to our RnA-seq nGS application.

Micromon is involved with the Victorian Platform technologies network, and was represented at trade and scientific meetings to promote the service to the broader scientific community.

facilities• Illumina GAllx Genome Analyzer• Illumina MiSeq• Illumina HiSeq (outsourced)• Illumina Cluster Station• Covaris S2 focused acoustic shearing system• Agilent Bioanalyzer 2100

capabilities and services• Project scoping and pricing• Sequencing library preparation• transcriptomics (mRnA sequencing, both

eukaryotic and prokaryotic)• Small RnA sequencing and discovery• DnA-protein interaction studies (ChIP-seq)• Methylation analysis (Bisulphite sequencing)• Bioinformatics• Closed-cell nucleic acid, cell and tissue

shearing• Quantitative real-time PCR

www.micromon.monash.org

Micromon


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MMI is a core facility supporting research in the life and biomedical sciences in all faculties and associated research institutes at three Monash university research precincts. there are four main units: the Advanced optical Imaging Facility and the BioeM Facility at the main Clayton campus, and smaller advanced optical imaging nodes at Monash Health translation Precinct (Clayton) and the AMReP (Prahran). the Advanced optical Imaging Facility is one of the largest in Australia and is specially designed to support live cell and functional imaging. In 2012, the BioeM Facility was expanded to include a new CryoteM Ramaciotti Centre for Structural Biology and it will bring signifi cant research capacity to cell and structural biology for Monash university and other Victorian universities.

facilities• optical brightfi eld and fl uorescence microscopy,

including polarisation microscopy• Stereo /dissection microscope imaging in

brightfi eld and fl uorescence modes• large area imaging/whole slide scanning for

both brightfi eld and fl uorescence• Automated widefi eld optical microscopy, with

cell incubation capabilities for time-lapse and live cell imaging. live cell microscopy is supported further by an in-house tissue culture facility

• Deconvolution microscopy• Confocal microscopy; based on upright and

invert confi gurations, these systems have either normal galvo scanning, or high-speed resonant or spinning disk scanning, as well as xy and z stage scanning

• Multiphoton microscopy: several instruments, with upright confi guration and heating chambers are available for cell, tissue and small animal work

• Fluorescence quantitation and imaging; including specialised fl uorescence analytical techniques such as FRAP, FRet, FCS, photoactivation, cell tracking and multiparameter imaging (5D microscopy)

• Fluorescecence correlation spectroscopy (FCS); FCS with sensitive single molecule detection is coupled with a confocal/multiphoton instrument, and cell incubation chamber

• Image analysis and visualisation laboratory; providing a comprehensive range of 2D and

3D software including Imaris, Volocity and MetaMorph

• transmission and Scanning electron Microscopy; with associated specimen preparation for resin sectioning and ultramicrotomy, as well as Cryo-techniques (high pressure freezing, freeze substitution, and cryo- ultramicrotomy)

• ImmunoeM, and correlative microscopy, including immuno-detection

• Imaging workshops for all of microscopy modalities and image analysis

capabilities and services• Microscopy training in instrument operation,

research methods and analysis• Provision of optical and electron Microscopes,

including operator training to self-use, for the functional and structural analysis of living and fi xed tissues in two or more dimensions

• Planning and supervision of projects, including general methodological approaches or specifi c protocols and applications

• expertise and support in advanced optical microscopy, including widefi eld fl uorescence and confocal microscopy, multiphoton microscopy, automated and time-lapse imaging, microscopy of live cells and tissues

• Correlative microscopy (extending optical microscopy to the level of the eM for structure or molecular localisation and identifi cation)

• optimisation of specimen preparation, immunolabelling, and staining for study by optical and electron Microscopy

• live Cell Imaging expertise • Image analysis, image reconstruction and

visualisation• Research collaboration

www.microimaging.monash.org

Monash Micro Imaging

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Monash’s Structural Biology Platform comprises two facilities: a state-of-the-art high-throughput protein crystallisation facility, and the Macromolecular X-ray Diffraction Facility. Crystal structures determined in the Facility can form the basis of a wide range of biomedical science from basic biology to the design of new pharmaceuticals.

During 2012 the Structural Biology Platform was heavily used by structural biology research laboratories within Monash university. this resulted in a record number of crystals being grown, and the determination of the crystal structures of targeted biomedical macromolecules.

the Rigaku CrystalMation Robot system is a fully integrated platform for macromolecular crystallisation. experiments are designed and controlled using the sophisticated Crystaltrak software package that also provides image analysis tools. two liquid-handling robots allow automated preparation of custom crystallisation formulations and setting-up of crystallisation trials. the plates are subsequently transferred to temperature-controlled incubators where crystallisation drops are automatically inspected for crystals and photographed according to allocated schedules. the researchers can access their results remotely via a web-based interface.

this high-throughput crystallisation facility can perform hundreds of crystallisation trials in a very short time frame, using 96-well plate format, non-contact nano-dispensing and as little as 100 nl of protein per plate. More than 5000 crystallisation plates can be incubated simultaneously in 8 incubators. High quality crystals produced in the Protein Crystallisation Facility can then be pipelined into the Macromolecular X-ray Diffraction Facility.

facilities• Rigaku Crystalmation system• Access to data analysis• High flux microfocus X-ray generators each

equipped with confocal optics, cryocooling and image plate detectors, all under computer control

capabilities and services• Automated screening of crystallisation

conditions• Design of optimisation experiments• Custom screen making and optimisation of

crystallisation experiments• Crystal screening• X-ray diffraction data collection• Image storage and analysis• Crystal structure determination

www.bioplatforms.monash.edu/bioplatforms/structural-biology.html

Structural Biology


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the Monash Gene targeting Facility (MGtF) has a proven capability in the creation of transgenic rodent models through eS cell transfection and DnA injection techniques including the use of BAC, YAC and viral transgenesis. the facility is able to provide additional advanced reproductive services including Intracytoplasmic Sperm Injection (ICSI) and in vitro fertilisation (IVF) procedures.

located within the facilities of the Monash Animal Research Platform (MARP), MGtF conducts the oocytes and blastocysts microinjections, embryo transfer to foster mothers and breeding of progeny to confi rmed germ line transmission.

the Monash Gene targeting Facility works closely with both the ARMI Gene Recombineering and the Murine embryonic Stem Cell Services to provide a comprehensive transgenic rodent production and delivery service.

capabilities and services• Mouse eS cell injection into blastocysts and

breeding of pups to chimera or heterozygous stage

• embryo pronuclear injection of DnA and rearing of progeny to tg founders or germ line.

• Pronuclear injection of BAC or YAC (hybrid or inbred strains) and rearing of tg founders to germ line

• ICSI-sperm-mediated transgenesis oocyte injection and rearing of tg founders to germ line

• ICSI and in vitro fertilisation and breeding of progeny

group membersmanagerDr Jose Gonzalez

scientifi c offi cerDr Zhongtao Du

technical staffMs Jessica trollope (from october)

Monash Gene targeting Facility

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Monash Antibody technologies Facility [MAtF) uses advanced robotic platforms to produce custom made, high quality, high affi nity mouse or rat monoclonal antibodies. using this technology, MAtF has the capacity to provide thousands of novel antibodies every year to a global clientele. Primary screening is by MAtF’s proprietary Antigen-MicroArray (AMA) technology, allowing specifi c selection of IgG secreting clones. AMA also enables screening against multiple proteins to identify specifi c binding patterns, while signifi cantly reducing the amount of antigen required compared with traditional methods.

facilities• 10 tecan robotic platforms• Genetix ClonePixFl• Remp robotic freezer• BioRad Profi inia Purifi cation System• ArrayJet Microarray Spotter• tecan Microarray Scanner

capabilities and services• Custom monoclonal antibody generation• Cell line cloning and stabilisation• Antibody purifi cation• Western blot• SPR analysis• elISA development

https://platforms.monash.edu/matf/

Monash Antibody technologies Facility


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governance

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Leadership advisory board the ARMI leadership Advisory Board (ARMIlAB) has an important role in helping the Institute succeed in achieving its objectives and strategic goals.

the lAB plays a supportive role in enhancing the reputation and positioning of the Institute with key stakeholders including business, government, media and the broader community

Specifi cally, ARMIlAB works closely with senior management to:• promote the vision, role and accomplishments

of the Institute among business, government, media and the broader community

• assist in the development of new ideas and initiatives to support the objectives of the Institute

• provide the Director and any steering bodies with feedback from an external perspective of the Institute.

the focus of ARMIlAB on contributing to the Institute achieving its objectives will be accomplished through: • advocacy• contributing experience and insight• supporting and, where appropriate, mentoring

the Institute’s Director and its leadership• supporting the Institute’s fundraising objectives

by assisting the Institute and Monash external Relations, Development and Alumni (eRDA) to build key philanthropic, donor and funding relationships.

board members Dr Janine Kirk, AM (Chair)

Dr Kirk, AM, is the lead Partner, Government & Public Sector at ernst & Young. Janine is also a member of the ernst & Young Area Advisory Board and Asia Pacifi c Area Council, a director

of the ernst & Young Foundation and was recently appointed leader, Community with responsibility for eY’s community and social responsible activities across Australia and new Zealand.

The Hon. Dr Kay Patterson (Deputy Chair)

Dr Patterson was a Senator for Victoria for 21 years. Prior to entering the Senate, Dr Patterson managed a small business before attending

university and attaining a PhD in Psychology. She taught at Sydney and Monash universities and held senior academic positions, including Chairman of the School of Behavioural Sciences at the lincoln Institute of Health Sciences (now at la trobe university).

The Hon. John Brumby

the Hon. John Brumby was Premier of Victoria from 2007 to 2010 and has immense experience in public life. He served for more than ten years as treasurer and then

Premier of Victoria, six years as leader of the Victorian opposition and seven years as Federal MHR for Bendigo during the period of the Hawke Government.

Mr Andrew Dyer

Mr Dyer is Chairman of the telecommunications Industry ombudsman Council and ItSS, and serves on the boards of the transport Accident Commission, BrightSource energy Australia,

the American Australian Association, the Australian Solar energy Society and the Good Foundation.

Committees

54Page


Ms Elizabeth Matthews

Ms Matthews has retired from a 20-year legal career with Corrs Chambers Westgarth, where she spent most of her years as a commercial litigator before progressing into internal

management positions. For the two years prior to her retirement, Ms Matthews was the national Manager of legal Support. Ms Matthews now works in a volunteer capacity in the Melbourne County Court with Court network.

Mr Tim Murphy

Mr Murphy has held senior roles across a range of industries in the public and private sectors from the Federal Government to pharmaceutical, medical research and biotechnology to

higher education, arts and tourism.

Dr Zita Unger

Dr unger has a distinguished career spanning 15 years as an evaluator, educator and entrepreneur, drawing on extensive knowledge of organisational development,

business acumen and governance to bring valuable contributions at the board level. She gained her doctorate in Sociology of education at the university of Melbourne.

Professor Nadia Rosenthal (executive Member)

Professor Rosenthal is the founding director of ARMI. Professor Rosenthal was previously Head of the european Molecular Biology laboratory in

Monterotondo (Rome) Italy, where she directed the eMBl Mouse Biology Program. She also holds a visiting professorship at the university of Western Australia. She has served on numerous study sections, advisory panels and editorial boards including the new england Journal of Medicine, where she was Consultant of Molecular Medicine.

Mr Michael Spiegel (executive Secretary)

Mr Spiegel is the Head, Strategic Development, and executive Secretary of the leadership Advisory Board for ARMI and director and founder of

Mirrorcone Pty ltd. Mr Spiegel joined ARMI in September of 2009 after co-founding and running the Monash Antibody technologies Facility, one of the world’s most sophisticated and automated novel antibody facilities.

group Leaders (armI faculty)committee membership• Dr James Bourne• Professor Peter Currie• Professor nadia Rosenthal• Associate Professor tiziano Barberi• Dr Julian Heng (Deputy Chair)• Dr Jan Kaslin• Professor Graham lieschke (Chair)• Professor Christophe Marcelle• Dr edwina McGlinn• Dr nicolas Plachta• Mr Silvio tiziani• Dr Claude Bernard (from Sept 2012)• Dr Jose Polo (from April 2012)• Dr Robin Hobbs (from Feb 2012)

meeting dates24 February30 March27 April25 May29 June27 July31 August28 September26 october7 December

governance

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safety committeecommittee membership• Ms Renae Hayle (Chair)• Ms Simone Heane (Secretary)• Dr lina Wang, Biosafety offi cer• Mr William Kwan, First Aid Coordinator• Dr Arianna nenci, Radiation Safety offi cer• Dr Dirk truman, Alternate Radiation Safety

offi cer• Mr Silvio tiziani, Chief operating offi cer• Mr Julian Cocks, Aquarium Manager• Mr David Hurst, Faculty oHS consultant• Mr Wouter Masselink, Student representative

(until April 2012)• Mr Ivan ng, Student representative

(from July 2012)

terms of referencethe committee helps implement new safety regulations handed down by the Monash occupational Health and Safety group and tries to ensure a safe working environment at ARMI. the Safety Committee comprises a critical element of ARMI’s Safety System, helping to ensure the Institute has a safe working environment and complies with relevant university and legislative requirements.

2012 highlightsthe committee focused this year on the university’s oHS Strategic Plan, which involved providing training of all new staff and post graduate students in:• Biosafety Module 1 (Microbiology)• Biosafety Module 2 (oGtR/AQIS).

the committee also organised training for new fi re wardens and the fl u vaccination for staff. two laboratory safety computers were also installed to enable ARMI staff and students to fi nd safety documentation quickly and without having to leave the lab area.

meeting dates21 February20 March24 April24 July18 September20 november

scientifi c services committeecommittee membership• Ms Renae Hayle (Chair), Manager, Resources

and Scientifi c Services• Ms Barb Bethune, technical offi cer, Scientifi c

Services• Ms Simone Heane, Secretary• Dr Gurpreet Kaur• Mr William Kwan• Dr Joly Kwek (until March 2012)• Mr Joseph Chen (from June 2012)• Dr olivier Serralbo• Ms Carmen Sonntag• Mr Zhengdong Qu• Ms Sony Varma• Dr lina Wang• Ms Krutika Wikhe (until June) • Ms lisa Wong• Ms Mia De Seram (from August)• Ms Jean tang (from August)• Dr Melissa Cooney, eS Cell Services (alternate)• Ms Jeanette Rientjes, Gene Recombineering

(alternate)

terms of referencethe role of this committee is to discuss matters including equipment requirements, laboratory issues, purchasing of laboratory consumables and reagents, and to determine ways to put into practice any new scientifi c requirements.

2012 highlights throughout the year the committee was involved in the purchase and maintenance of shared laboratory equipment. they also set new guidelines for the use of communal areas and helped with the integration of a new lab group into the north shared laboratory space.

they were integral in updating the chemical database of each lab group and with the help of the workshop, developed a safer way to use a new piece of laboratory equipment.

lastly, the committee was vital in helping to maintain the laboratory equipment through a system of shared responsibility.

meeting dates7 February28 March6 June15 August10 october

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scientific advisory committeeProfessor Dame Kay Davies, DBe, FMedSci, FRS, university of oxford

Professor Sir Magdi Yacoub, FRCS, FRS, Imperial College london and Harefield Heart Science Centre

Professor Peter Rigby, FRS, FMedSci, the Institute of Cancer Research, uK

Professor eric olson, university of texas Southwestern Medical Center, Dallas, uSA

Professor nadia Rosenthal, ARMI and eMBl Australia

Mr Silvio tiziani, ARMI and eMBl Australia

terms of referencethe Committee provides advice to the Director, in particular with regard to scientific proposals and on the preparation and realisation of the scientific programme of the Institute. It performs its task in due cooperation and in consultation with the Director, seeking the advice of experts where appropriate.

highlightsMembers of the Committee met for the first time as a group during the Scientific Retreat in May to review progress with the Institute and make recommendations on future scientific directions including recruitment.

fishcore management committee/ fishcore user group fishcore management committee membership• Professor Graham lieschke (Chair)• Professor Peter Currie• Mr Julian Cocks • Dr Jan Kaslin • Dr Heather Verkade (Faculty of Science)• Dr Robert Bryson-Richardson (Faculty of

Science)

terms of referencethe Committee is responsible for overseeing the facility. It works with management to ensure a technically proficient and sustainable facility that meets the needs of researchers.

meeting dates19 January16 February

the composition of the FishCore Management Committee was changed during the year to incorporate more feedback from the users of the facility. the committee is now called the FishCore user Group.

fishcore user group membership• Mr Julian Cocks (Chair)• Mr Rodney Glanvill• Ms Kym Grundy (until July)• Mr Pitra Yudhyantara • Ms Sony Varma • evelyn Yip • Aminah Griousoh • Raquel Vaz • Mo Zhao • Ms Carmen Sonntag• Brendan Wilding • Mr eren Yilgor• Dr Milena Furtado• Dr Jan Kaslin• Mr Wade Moore• Dr Patricia Jusuf

meeting dates17 August12 october9 november7 December

2012 highlights• A new meeting format led to improved

communication and synergy between FishCore staff and researchers.

• Incentives were introduced to reduce labour, energy and water waste through a conscious strategy to use appropriately sized fish holding tanks for individual research projects.

• FishCore management and procedures were simplified to provide researchers with better services.

governance

57Page


higher degree by research (hdr) committeethe HDR program has continued to develop since its inception in 2011. PhD student enrolments rose from six to eleven.

In 2012, ARMI continued to attract Honours students with six students enrolling.

undergraduate students had the opportunity to undertake research projects as either vacation scholarships students or via the “research in action” unit, highlighting ARMI’s commitment to contribute to undergraduate teaching.

the HDR Committee continues to provide support to the students and information and advice to the ARMI Faculty.

committee membership• Associate Professor James Bourne, HDR

Coordinator (Chair)• Ms Jane McCausland, Student Programs

Coordinator• Professor Graham lieschke, Faculty member• Dr edwina McGlinn, eMBl Australia Group

leader• Dr Mauro Da Costa, Honours representative• Dr Cristina Keightley, uRoP Coordinator• Mr Wouter Masselink, PhD student

representative • Mr Silvio tiziani, Chief operating offi cer

executive hdr committee membership• Associate Professor James Bourne, HDR

Coordinator (Chair)• Ms Jane McCausland, Student Programs

Coordinator• Professor Graham lieschke, Faculty member• Dr edwina McGlinn, eMBl Australia Group

leader

terms of referencethe role of the ARMI HDR Committee is to provide independent guidance, support and mentoring to research students, supervisors and members of the institute.

the role of the executive committee is to handle issues pertaining to the recruitment of students, ranking of scholarship applications, progress of students and issues of a confi dential nature in relation to HDR coordination.

2012 highlightsAll students participated in the ARMI Scientifi c retreat held in May with a poster presentation of their research.

the students in ARMI’s inaugural PhD cohort all successfully achieved their confi rmation of candidature.

All the Honours students achieved an H1, with special mention to linh ngo who received an award from the Department of Anatomy and Developmental Biology as the top overall BMS and Science Honours student.

A student lunch was held to celebrate the launch of the student committee.

meeting dates9 February1 March10 May31 May (Student meeting)5 July3 August (Individual meeting with HDR Coordinator)4 october19 november (executive HDR Committee)

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offi ce of the directorMr Silvio tiziani, Chief operating offi cerMs Barbara Bethune, technical offi cer, Scientifi c ServicesMs laura Crilley, executive offi cerMs Renae Hayle, Manager, Resources and Scientifi c ServicesMs Simone Heane, Administrative AssistantMs Penny Rowlett, Finance offi cerMs lisa Ryan, executive Assistant, eMBl Australia (until June)Ms Jane McCausland, Student Program Coordinator Mr Mike Spiegel, Head of Strategic DevelopmentMs Michelle Ahlquist, executive Assistant (from August)

core facilitieses cell genetics servicesDr Arianna nenci, ManagerMs Wendy Chua, Research AssistantDr Melissa Cooney, Research AssistantDr Dirk truman, Research Fellow

fishcoreMr Julian Cocks, Aquarium ManagerMr eren Yilgor, Aquarium technicianMr Rodney Glanvill, Aquarium technicianMr Pitra Yudhyantara, Aquarium technicianMs Kim Grundy (until August)Mr Maik FiedelMs emma CarlosMs Jamie MihelakosMs olivia FerraroMs tanya HynesMs natalie Bien-IzowskiMs natalie Wong

gene recombineeringMs Jeanette Rientjes, DirectorMs Margareta Go, Research AssistantMs Susan thomas, Research Assistant

research groupsbarberi groupAssociate Professor tiziano Barberi, Group leaderDr Isabella Mengarelli, Research Fellow (until September)Ms Bianca Borchin, PhD student (from January)Mr Joseph Chen, Research Assistant (from March)

bourne groupDr James Bourne, Group leader Dr Jihane Homman-ludiye, Research FellowMr William Kwan, Research AssistantMr leon teo, PhD studentMs Claire Warner, PhD studentMs Skye Kinder, Honours studentDr tobias Merson, ARMI affi liateProfessor Anita Hendricksen, ARMI affi liateDr Julie thacker, ARMI affi liate (from June 2012)

currie groupProfessor Peter Currie, Deputy Director, Group leaderDr Joachim Berger, Research FellowMs Silke Berger, Research AssistantDr Catherine Boisvert, Research FellowMs ophelia ehrlich, PhD studentDr Yona Goldshmit, Research FellowMr David Gurevich, PhD studentDr tom Hall, Research Fellow (until January)Dr Patricia Jusuf, Research FellowMr Zhenhua li, PhD student (from March)Mr Wouter Masselink, PhD studentMr Phong nguyen, PhD studentMs Carmen Sonntag, Research Assistant

ARMI team

governance

59Page


heng groupDr Julian Heng, Group leaderDr Matilda Haas, Research FellowMr Ivan ng, PhD studentMr Zhengdong Qu, Research Assistant Ms Shanshan li, Research FellowMs linh ngo, Honours student

hobbs groupDr Robin Hobbs, Group leaderMs Mia De Seram, Research Assistant (from June)

Lieschke groupProfessor Graham lieschke, Group leaderDr Felix ellett, Research FellowDr Cristina Keightley, Research FellowMs Sony Varma, Senior Research AssistantMr Sultan Alasmari, PhD student (from october)Mr Vahid Pazhakh, PhD student (from november)Ms emily Scott, uRoP student (from July)Dr Chieh-Huei Wang, Research Fellow (from october)Mr Johannes Wittig, Visiting student (from August)

kaslin groupDr Jan Kaslin, Group leaderMs Krutika Wikhe, Research Assistant (until June)Ms Frisca Frisca, Visiting PhD student (from September)Ms Jean tang, Research AssistantMr Sunil Kumar, Casual Research Assistant (from August)Mr Raphael Park Chae, Casual Research Assistant (from January)Ms Celia Vandestadt, uRoP student (from January)

marcelle groupProfessor Christophe Marcelle, Group leaderDr David Salgado, adjunct Research Fellow (from August)Dr olivier Serralbo, Research Fellow Mr Cyril Picard, PhD student/Research Assistant (from october)Dr Manuel Pele, Adjunct Research Fellow (from July)Dr Anne Rios, Research Fellow/ARMI Affi liateMr Daniel Seiero Mosti, PhD studentDr Ashvind Prabahran, Research Fellow/ARMI Affi liate (until September)Mr Jeremy ng Chi Kei, Honours studentMs nadege Veron, Research Assistant

Mr Mark Sedrak, uRoP studentDr Federico Calhabeu, Research Fellow (from April)Dr Claire Hirst, Research Fellow (from September)

mcglinn groupDr edwina McGlinn, Group leaderDr Alysha Heimberg, Research FellowMs lisa Wong, Research AssistantMr eamon Coughlin, PhD studentDr Jesus Casanova, Research Fellow (from July)Mr Janus Jacobsen, ARMI affi liate (from January to July)

plachta groupDr nicolas Plachta, Group leaderDr Gurpreet Kaur, Research FellowMr Juan Silva, Research AssistantDr Juan Gonzalez, Research Fellow (from March)Dr Melanie White, Research Fellow (from May)Dr Jennifer Zenker, Research Fellow (from December)

polo groupDr Jose Polo, Group leaderDr Sue Mei lim, Research FellowDr Christian nefzger, Research FellowSara Alaei, PhD studentJaber Firas, undergraduate student

rosenthal groupProfessor nadia Rosenthal, Group leader/Director, ARMIDr Minna-liisa Anko, Research Fellow/ARMI Affi liateDr Mauro Costa, Research FellowDr Milena Furtado, Research FellowDr James Godwin, Research FellowMr nicholas lam, Research Fellow/ARMI Affi liate Dr ekaterina Salimova, Research Fellow (from July)Dr Alex Pinto, Research FellowMs Joelle Perera, Honours studentDr lina Wang, Research FellowMr Drew Kuraitis, visiting student/ARMI Affi liate (from September)Ms Anjana Chandran, Research Assistant

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nationalities

governance

nationalitiesAUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE SWEDEN ITALY JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN DENMARK IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN TAIWAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE HOLLAND AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA DENMARK GERMANY SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN DANISH HUNGARIAN UK ISRAELI MALAYSIAN SWITZERLAND RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN HOLLAND DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND ENGLAND SWEDEN UK GERMANY BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH USA ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE SWITZERLAND CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN SAUDI FRANCE AMERICAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA JAPAN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH ISRAELI MALAYSIAN IRANIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN ISRAELI SPAIN AUSTRALIAN ITALY FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER TAIWAN INDIAN SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIANSERBIAN IRAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN ISRAEL JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND MALAYSIA SINGAPORE BANGLADESH SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK SINGAPORE ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN MALAYSIA CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK BRAZIL ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN ISRAELI TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH SERBIAN HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL NEW ZEALANDMALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN

STAFF AND STUDENTS IN 2012 CONSISTED OF 32 DIFFERENT NATIONALITIES…

…AND WERE RECRUITED FROM 19 DIFFERENT COUNTRIES

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AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE SWEDEN ITALY JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN DENMARK IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN TAIWAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE HOLLAND AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA DENMARK GERMANY SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN DANISH HUNGARIAN UK ISRAELI MALAYSIAN SWITZERLAND RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN HOLLAND DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND ENGLAND SWEDEN UK GERMANY BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH USA ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE SWITZERLAND CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN SAUDI FRANCE AMERICAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA JAPAN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH ISRAELI MALAYSIAN IRANIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN ISRAELI SPAIN AUSTRALIAN ITALY FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER TAIWAN INDIAN SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIANSERBIAN IRAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN ISRAEL JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND MALAYSIA SINGAPORE BANGLADESH SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK SINGAPORE ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN MALAYSIA CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK BRAZIL ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN ISRAELI TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK 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NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN 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RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN AUSTRALIAN FRENCH AMERICAN GERMAN NEW ZEALANDER SINGAPOREAN BRITISH DUTCH INDIAN ITALIAN CHINESE JAPANESE SPANISH ARGENTINIAN FINNISH INDONESIAN IRANIAN ISRAELI MALAYSIAN MEXICAN VIETNAMESE BANGLADESHI CANADIAN CUBAN DANISH HUNGARIAN ISRAELI MALAYSIAN RUSSIAN SAUDI SERBIAN TAIWANESE AUSTRALIA USA FRANCE ITALY BRAZIL JAPAN GERMANY HOLLAND IRAN SWEDEN UK BANGLADESH DENMARK ENGLAND ISRAEL MALAYSIA SINGAPORE SPAIN SWITZERLAND TAIWAN AUSTRALIAN

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fInancIaLs

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2012 2011 2010 2009

($,000) ($,000) ($,000) ($,000) noteIncome

Research Grants 4,831 4,090 1,363 1,192

Donations 1

Fee-for-service 307 398 318 349

IGS/RIBG & research support 1,205 861 581 620

teaching 308 0 0 0

FMnHS & DVC(R) contribution 2,885 4,047 3,432 2,404

DVC(R) Advance on 2011 383

other Income 70 67 332 196

total Income 9,607 9,464 6,409 4,761

Salary Expendituretotal Salary expenditure 7,193 6,353 4,939 3,267

Non-salary Expenditure 1

Capital expenditure 88 20 44 66

Book & library 17 9 14 5

Building & Property -25 -12 59 22 2

Communications Related 77 73 145 154

Computer Related 35 39 86 78

equipment Related 73 122 -31 56 2

Finance & Admin 29 21 21 9

lab & operating 1,502 921 810 721

Motor Vehicle 5 9 9 9

Print & Stationery 54 90 72 58

Staff Related 73 -77 4 214 2

Student Scholarship 232 158 31 7

travel Related 360 318 245 187

other expenses 111 68 -31 81 2

total non-salary expenditure 2,630 1,757 1,477 1,667

Total Expenditure 9,823 8,110 6,416 4,934

Net Operating Result -216 1354 -8 -172

Previous Year Carry forward 1133 -221 -213 137 3

Closing Balance 917 1133 -221 -36

notes 1 Does not include central services overheads (e.g. HR, Finance, It and space/rental)2 Cost recoveries3 2010 Carry forward adjusted from 2009 close

ARMI Financial Report 2012

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actIvItIes

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visitorsARMI hosted visitors from international and Australian organisations. ARMI also welcomed many visitors who presented seminars during 2012—see external Seminar Series (below).

februaryProfessor Hiroaki Kitano visited 12–13 March 2012, during his visit for the signing of an Mou with eMBl Australia, and met with ARMI researchers from the Rosenthal and Plachta Groups to discuss potential collaborations.

juneA delegation from King Abdulaziz university, Saudi Arabia, visited and toured ARMI, hosted by Mr Silvio tiziani. the delegation included Professor Abdulmonem Al-Hayani, Secretary Medical Deans Committee and Vice Dean for Basic Medical Sciences.

julyA delegation from the Monash university Medical Foundation (MuMF) visited and toured ARMI, hosted by Professor Peter Currie. the delegation included:• Dr lara Grollo• Mr Jeremy nestel• Mr lewis Johnston.

A delegation by Southeast university China (Monash university’s Graduate School in China) visited ARMI, hosted by Professor Peter Currie.

Dr Silke Schumacher of eMBl, Heidelberg, visited the eMBl Australia secretariat offi ce at ARMI and met with Mr Silvio tiziani, executive Director, and Group leaders Dr nico Plachta and Dr eddy McGlinn.

A delegation from underworks Pty ltd and executive Compass Management Consultants visited and toured ARMI, hosted by Professor Peter Currie. the delegation included:• Mr Sam, Managing Director, underworks Pty ltd• Dr Dan evans, Founder and Principal, executive

Compass Management Consultants.

A delegation from the university of Warwick, uK, visited and toured ARMI, hosted by Mr Michael Spiegel.

the delegation included:• Sir George Cox, Pro Chancellor, university of

Warwick• Professor tim Jones, Pro Vice-Chancellor,

Knowledge transfer and Business engagement and Research (Science and Medicine)

• Professor Peter Winstanley, Dean of Medicine.

augustDr Matthias Hentze, Associate Director, eMBl hosted by Mr Silvio tiziani, visited and toured ARMI & eMBl Australia.

A delegation from technion, Israel Institute of technology, visited and toured ARMI, hosted by Professor Peter Currie. the delegation included Professor Peretz lavie, President, technion, Israel Institute of techology.

Professor Jussi Helppi, Head of Biomedical Servies and Facilities, Max Planck Institute of Molecular Cell Biology and Genetics, Germany, visited and toured ARMI. He also gave a presentation (see external Seminars).

septemberA delegation from the national Research Foundation, uAe, visited and toured ARMI. the delegation included:• Dr Ahmed Alosi, Research Program Manager• Dr Husam Sultan Al-ulama, Director.

octoberA delegation from Dammam university, Saudi Arabia, visited and toured ARMI and FishCore, hosted by Mr Silvio tiziani. the delegation included:• Alsultan, Ali Ibrahim, Dean of the College of

Medicine• Motabagani, Mohamedakram Hassan,

Chairman of Curriculum Committee, Anatomy• Al elq, Abdulmohsen Habib, Member of

Curriculum Committee, Internal Medicine• Sebiany, Abdulaziz Mohammed, Member of

Curriculum Committee, Family and Community Medicine

• Alshaikh, Mona Hammoud, Member of Curriculum Committee, Physiology.

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events februaryJohn Brumby, former Victorian Premier and ARMI board member, published an opinion piece “Zebrafish help put us ahead of pack” in the Age 28 March (http://www.theage.com.au/federal-politics/society-and-culture/zebrafish-help-put-us-ahead-of-pack-20120327-1vwfa.html).

march A Memorandum of understanding (Mou) was signed with SBI and Monash university by Professor Hiroaki Kitano (SBI) and Professor Richard larkins (Monash university) at the Melbourne Convention and exhibition Centre.

aprilMr Silvio tiziani and Dr Sarah Boyd gave a presentation to the Defence Science and technology organisation (DSto) at Fisherman’s Bend. they explained the background to SBI Australia and eMBl Australia and discussed possible collaborations and linkages with DSto.

may–juneARMI research staff attended a Scientific Retreat at Silverwater Resort, near Phillip Island.

the retreat was attended by two members of the ARMI Scientific Advisory Committee, Professor Peter Rigby, Royal Cancer Hospital, london, and Professor Dame Kay Davies, university of oxford.

julyARMI hosted a teAM VIC (tissue engineering and Matrix Group of Victoria) seminar on Cell therapy, supported by CSIRo and the Small technologies Cluster:• Dr David James, Senior Director, life Sciences

and Pharmaceutical, Invetech Pty ltd, spoke on “Cell therapy: Commercialisation—Creating a Roadmap for Success”.

• Dr Ray Wood, Managing Director, Cell therapies Pty ltd, spoke on “Research translation: from Bench to Clinic and Beyond—the regulatory and cGMP requirements”.

augustARMI hosted a next-Generation Sequencing Bioinformatics Workshop, which was organised by AGRF and eMBl Australia. the workshop gave biologists who are new to analysing next-Gen Sequencing data a snapshot of bioinformatics and data analysis.

Monash IVF, ARMI and the Systems Biology Institute Workshop. Researchers from ARMI and Monash IVF explored collaborative opportunities arising from the research conducted by Dr nico Plachta and Professor Hiroaki Kitano.

octoberA delegation from Monash/ARMI visited Japan for signing of agreement with SBI. Delegates included:• Professor edwina Cornish, Senior Deputy

Vice-Chancellor and DVC (Research) , Monash university

• Dr Sarah Boyd, ARMI• Mr Silvio tiziani, ARMI• Professor Paul Bonnington, Director,

e-Research, Monash university• Mr Steve Quenette, Maths, Monash university• Professor Kerry Hourigan, Director, Division of

Biological engineering, Monash university• Professor nadia Rosenthal, Director, ARMI.

novemberARMI hosted a teAM VIC (tissue engineering and Matrix Group of Victoria) seminar on neural Repair, supported by Monash university and the Small technologies Cluster. Speakers included:• Dr James Bourne, Group leader ARMI, Monash

university, on “Models of Injury in the non Human Primate: A new era for treatment Strategies”

• Associate Professor John Forsythe, Department of engineering, Monash university, on “neural engineering Solutions for Brain Repair”

• Professor Jeffrey Rosenfeld, Department of Surgery, Central Clinical School, Monash university, on “Bringing new Brain Injury treatments to the Clinic”.

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armI in the news

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external seminar seriesjanuaryProfessor Patrick tam, embryology unit, Sydney Medical School, university of Sydney: “Intersection of transcriptional and signalling activity for tissue morphogenesis in mouse embryos”

marchProfessor Justin Cooper-White, Australian Institute for Bioengineering and nanotechnology, university of Queensland: “tailored surfaces, scaffolds and diagnostic cell-based microfl uidic platforms for small molecule screening, stem cell expansion and controlled tissue genesis”

Professor Francis Stewart, tu Dresden, Germany: “Reprogramming of the epigenome during early mouse development”

Dr James Briscoe, Division of Development neurobiology, national Institute for Medical Research, london: “the Gene Regulatory logic of Sonic Hedgehog Morphogen Signalling”

mayDr Sam Wormald, Cell and Molecular Biology laboratory, WeHI Systems Biology & Personalised Medicine: “Systematic mapping of genetic interaction networks governing embryonic stem cell self-renewal”

Dr Judith Anderson, Professor and Head of Department of Biological Sciences, university of Manitoba, Canada: “Muscle Regeneration from Finish to Start, and Back”

Dr Karina Yaniv, Weizmann Institute, Israel: “Fishing for novel Regulators of Developmental Angiogenesis”

Professor eldad tzahor, Weizmann Institute, Israel: “Head & Heart Muscle Development and Regeneration”

Dr Vladimi Korzh, Institute of Molecular and Cell Biology, A*StAR, Singapore: “Mechanisms of late neurulation as refl ected by the roof plate development”

juneProfessor John Mattick, executive Director, the Garvan Institute of Medical Research, Sydney: “RnA at the epicentre of human development”

julyProfessor Greg Stuart, the John Curtin School of Medical Research, Canberra: “How input from the eyes is processed by the brain”

augustProfessor Hiroaki Kitano, the Systems Biology Institute, tokyo, Japan: “Act Beyond Borders”

Professor Melissa little, IMB Kidney Research laboratory, the university of Queensland: “the Kidney from morphogenesis to regeneration and back again”

Dr Jussi Helppi, Head of Biomedical Services, Max Planck Institute of Molecular Cell Biology and Genetics: “Centralised core services and facilities is the key for success”

septemberDr natasha Harvey, Centre for Cancer Biology, lymphatic Development lab, SA Pathology: “lymphatic Vascular Morphogenesis—From Progenitor Cells to Functional Vessels”

Associate Professor Gary Hime, Dept of Anatomy & neuroscience, university of Melbourne: “Genetic analysis of the regulation of stem cell differentiation by transcriptional and translational repressors”

octoberProfessor Miranda Grounds, School of Anatomy and Human Biology, university of Western Australia: “From Symbiosis to Sarcopenia: around 40 years of Skeletal Muscle Research”

novemberDr Karuna Sampath, Dept of Biological Sciences, national university of Singapore, Singapore: “Maternal Control of embryogenesis”

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Internal seminar seriesFor 2012 the ARMI external Speaker Series was expanded to include presenters from other units located in Building 75, including the Monash Immunology and Stem Cell laboratories (MISCl), as well as CSIRo. these groups include close colleagues and collaborators to ARMI and their inclusion in the seminar series helped to identify additional collaborative opportunities.

Presenter Designation Seminar Date Presentation Title

Professor Francis Stewart

Special Speaker 7 March Reprogramming of the epigenome during early mouse development

Dr Juan Carlos Fierro-Gonzalez

Research Fellow 14 March How tRX-1 contributes to maintaining survival in Caenorhabditis elegans

Dr Joachim Berger Postdoctoral Fellow

14 March Identifi cation and Characterisation of a Zebrafi sh Chaperonin Mutant

Dr Cristina Keightley Research Fellow 4 April unravelling the role of ZBtB11 in haematopoiesis

Dr Robin Hobbs Group leader 11 April transcription factor networks underlying germline development, maintenance and cancer

Dr Julian Heng Group leader 18 April understanding the neurobiology of intellectual disability to improve neuro-regenerative medicine

Dr James Godwin Senior Research Fellow

2 May Adult salamander regeneration is immunologically regulated

Dr Graham lieschke Group leader 9 May Imaging infl ammation in vivo in zebrafi sh

Professor nadia Rosenthal

Group leader 16 May Immune modulation of regeneration

Dr Matilda Haas Research Fellow 23 May Genetic control of cerebral cortex development and maturation

Dr Scott Coutts Genomic Sequencing

23 May Micromon, the Monash university DnA Sequencing Core Facility: Services, Applications and Access

Dr James Bourne Group leader 6 June Mapping neuroplasticity in the visual system

Dr Mauro da Costa Research Fellow 20 June of mice and man: deciphering nKX2-5 function in cardiomyopathy

Dr edwina McGlinn Group leader 27 June lineage specifi c regulation of Hox output by microRnAs

Ms Jessica Morison PhD Student 4 July the immunogenicity of stem cells and thymus-based strategies to minimise immune rejection.

Dr Roland (Runzhe) Shu

Research Fellow 18 July APP biology in neural stem cell differentiation and AD pathology

Dr nicolas Plachta Group leader 25 July Imaging mammalian development

Professor Peter Currie

Group leader 1 August Modelling muscle disease and regeneration in zebrafi sh

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Presenter Designation Seminar Date Presentation Title

Dr Chris Siatskas Research Fellow 8 August MSCs, the good the bad and the ugly

Dr Mauro Costa Research Fellow 8 August Generation and characterisation of human embryonic set cell-derived endothelial cells (heSC-eCs)

Dr Catherine Boivert Research Fellow 15 August evolution of myogenesis in vertebrates: Insights from the elephant shark

Dr tracy Heng Research Fellow 29 August How do multipotent mesenchymal stromal cells inhibit allergic airway hyperresponsiveness?

Dr Andrew Holland Research Fellow 29 August Pathways to Pancreas

Associate Professor tiziano Barberi

Group leader 5 September the directed differentiation of human pluripotent stem cells.

Dr Yona Goldshmit Research Fellow 12 September Blocking lysophosphatidic acid (lPA) after spinal cord injury improves outcome

Professor Sharon Ricardo

Group leader 12 September Cellular-based therapies for kidney regeneration and repair

Dr Jose Polo Group leader 26 September unveiling the reprogramming process

Dr Patricia Jusuf Research Fellow 3 october neuronal subtype specification and lineages within the developing zebrafish retina

Dr Jihanne Homman-ludiye

Research Fellow 10 october Guidance molecules contribute to patterning visual cortical areas

Dr David elliott Research Fellow 17 october Building the human cardiac cell lineage tree: identifying the initial shoots.

Dr Cyril Picard Research Assistant

24 october two distinct muscle progenitor populations coexist throughout amniote development

Associate Professor Ann Chidgey

Deputy Group leader

24 october limitations and strategies for thymic epithelial cell regeneration

Dr Gurpreet Kaur Research Fellow 31 october Searching for DnA targets in the mouse embryo

Dr Alex Pinto Research Fellow 7 november Immunological basis for cardiac senescence

Dr Milena Furtado Senior Research Fellow

14 november Insights into cardiac fibroblast identity

Professor Claude Bernard

Group leader 14 november neural differentiation of multiple sclerosis-specific induced pluripotent stem cells as a novel approach for disease modelling and drug discovery

Dr Alex Pinto Research Fellow 28 november Immunological basis for cardiac senescence

Dr Martin Short PhD Student 28 november Paracrine signalling by Mesenchymal Stem Cells and Amnion epithelial Cells

Professor Christophe Marcelle

Group leader 5 December unorthodox notch and Wnt signalling in early myogenesis

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research activitieseditorial boards

ARMI Rep Publication Role Since

nadia Rosenthal Developmental Biology editorial board 2001

BioMednet (mouse models of diseases reviews)

Guest editor 2001

Developmental Dynamics editorial Board 2003

Rejuvenation Research editorial Board 2004

Disease Models and Mechanisms Founding editor 2007

Stem Cell Research and therapy editorial Board 2010

Differentiation editor-in-Chief 2012

Peter Currie Development editorial advisory board 2006

Developmental Biology editorial advisory board 2011

Christophe Marcelle Developmental Dynamics Member, editorial Board 2002

Developmental Biology Member, editorial Board 2007

edwina McGlinn nature Peer reviewer/guest 2010

Developmental Biology Peer reviewer/guest 2010

Developmental Dynamics Peer reviewer/guest 2010

Gene expression Patterns Peer reviewer/guest 2009

Journal of Molecular Histology Peer reviewer/guest 2011

Plant Molecular Biology Peer reviewer/guest 2012

Mechanisms of Development Peer reviewer/guest 2008

Current Genomics Peer reviewer/guest 2012

Julian Heng open Journal of Cell Biology Peer reviewer/guest 2012

Journal of experimental Medicine Ad hoc reviewer 2005

tiziano Barberi Stem Cells Ad hoc reviewer 2006

BMC Developmental Biology Ad hoc reviewer 2009

Stem Cell Research Ad hoc reviewer 2009

translational neuroscience Associate editor 2011

James Bourne experimental Brain Research Co-editor 2010

early Human Development editorial Board 2007

the open Zoology Journal editorial Board 2007

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advisory boards and committees

Name Organisation Board/C’ttee name Role Since

nadia Rosenthal Scientific Advisory Committee, Genethon, Paris

Scientific Advisory Committee

Member 2000

Scientific Advisory Board, Harefield Research Foundation, london

Scientific Advisory Committee

Member 2001

eMBl representative, european life Sciences Forum (elSF)

eMBl representative

2001

International Mouse Mutagenesis Consortium

Member 2002

european Group on life Sciences (eGlS)

Member 2002

International Society of Differentiation

President-elect 2002

Institute of Advanced Studies, university of Western Australia

Scientific Advisory Board

Member 2005

Keystone Symposia Scientific Advisory Board

Member 2005

Center for Molecular Medicine, Vienna


Member 2007

Institute of Molecular Biology and Biotechnology, Heraklion, Crete


Member 2008

Max Planck Institute Scientific Advisory Board

Member 2012

Peter Currie Development editorial advisory board

Member 2006

45th meeting of the Japanese Society for Developmental Biology

Advisory Committee Member 2012

Global Facioscapulohumeral Muscular Dystrophy Association


Member 2008

Human Frontiers Science International fellowship committee

Member 2005

International steering committee on zebrafish research

Founding member

2007

James Bourne neurosciences Victoria ltd Scientific Advisory Committee

Member 2008

Christophe Marcelle

Monash Micro Imaging Scientific Advisory Board

Member 2011

Robin Hobbs Monash early-Career Researcher

Steering Committee Member 2012

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conference organising committees

Name Conference Role

Peter Currie 10th International Zebrafi sh Meeting, Madison Wisconsin, uSA, 2012.

organising Committee

45th meeting of the Japanese Society for Developmental Biology May 2012, Kobe Convention Center Japan

Advisory Committee

Julian Heng Fifth Melbourne Cell and Developmental Biology meeting of the Australia and new Zealand Society for Cell and Developmental Biology

organiser

ARMI external Seminar Series Chair

Professor ed Manser (IMB, Singapore), speaker for the Monash Developmental Biology eminent Speakers Program

organiser and host

Symposia on neural Development, ComBio 2012, Adelaide, Australia

organiser

memberships

Name Society Role Since

nadia Rosenthal American Society for Biochemistry and Molecular Biology

Member 1988

American Society for Cell Biology Member 1992

Society for Developmental Biology Member 1994

Society for Developmental Biology Board of trustees 1996

Society for Developmental Biology northeastern Representative

1997

Australia and new Zealand Society for Cell and Developmental Biology

Member 2001

International Society of Differentiation Member 2002

european Molecular Biology organization (eMBo) Member 2002

Peter Currie Australian and new Zealand Society of Cell and Developmental Biologists

Member 2003

ARC/nHMRC Research network in Genes and environment in Development

Founding member 2004

Society for Muscle Biology Member

International steering committee on zebrafi sh research

Member 2007

edwina McGlinn Australian and new Zealand Society for Cell and Developmental Biology

Member 2012

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Name Society Role Since

Christophe Marcelle

Developmental Dynamics editorial Board Member

2002

Developmental Biology editorial Board Member

2007

Monash Micro Imaging Scientific Advisory Board

Member 2010

Robin Hobbs Society for Reproductive Biology Member 2012

Graham lieschke Haematology Society of Australia & new Zealand Member 1991

Medical oncology Group of Australia Member 1991

Clinical oncology Society of Australia Member 1992

American Society of Hematology Member 1992

Australian Medical Association (AMA) Member 1997

Australian and new Zealand Society for Cell and Developmental Biology Inc (AnZSCDBI) (President 9/2004-9/2006)

Member 1997

Society for Developmental Biology Member 2003

national Association of Research Fellows Member 2009

euFishBioMed (CoSt action BM0804) Member 2010

International Society of experimental Hematology Member 2010

Julian Heng Research translation Faculty, the national Health and Medical Research Council of Australia

Member 2012

International Society for neurochemistry Member 2011

the Australian and new Zealand Society for Cell and Developmental Biology (AnZSCDB)

Member 2008

Australian neuroscience Society (AnS) Member 2008

tiziano Barberi new York Academy of Sciences Member

International Society for Stem Cell Research Member

the Science Advisory Board Member

James Bourne International Society for Developmental neuroscience

Member 2006

Australian Society for Medical Research Member 2004

Society for neuroscience, uS Member 2001

Australian neuroscience Society Member 2000

Biochemical Society, uK Member 1995

Royal College of Science, uK Member 1994

Jan Kaslin Society for neuroscience Member 2001

Federation of european neuroscience Societies Member 2011

Brain Research Society of Finland Member 2011

Australian neuroscience Society Member 2011

German neuroscience Society Member 2010

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memberships (cont)

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awards / honours / prizes & travel awards

Name Awarded By Award Value

Yona Goldshmit ARMI travel Grant $1,000

Christina Keightley ARMI travel Grant $1,000

Robin Hobbs erasmus Mundus, eu erasmus Mundus Invited Scholar

€2,500

Monash university larkins Fellowship

teaching

Name Program Since

Julian Heng, lecturer, Monash university

DeV3990 Action in Developmental biology research project

2011–2012

Gen3990 Genetics in Action Research Project

MeD4101 Honours Degree in Bachelor of Medical Science

DeV3032 Stem cells and the foundations of life

DeV3022 Developmental pathways to health and disease

edwina McGlinn, Guest lecturer, Monash university

Dev3011 Fundamentals of developmental processes

2011–2012

Dev2011 early human development from cells to tissues

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conferences and seminars

Name Name of Meeting Location Role

edwina McGlinn

Combio 2012 Adelaide, South Australia

Invited speaker

CoSt Action Meeting – Hox and tale transcription Factors in Development and Disease

Barcelona, Spain Invited speaker

lorne Genome meeting lorne, Victoria Invited speaker

nicolas Plachta

Hunter Meeting Hunter Valley, nSW Invited speaker

Christophe Marcelle

Frontiers in Muscle Biology new York, uSA Invited speaker

Israeli Society for Developmental Biology

Israel Keynote speaker

the 12th Hunter Meeting Hunter Valley, nSW Invited speaker

Robin Hobbs the Annual Scientific Meeting of the endocrine Society of Australia and the Society for Reproductive Biology

Queensland Invited speaker

Graham lieschke

Dr von Haunersches Kinderspital, 19 March, “Acute inflammation in a myeloperoxidase-deficient zebrafish mutant”

Munich, Germany Grand Rounds Seminar

2nd european Zebrafish PI Meeting (eZPM 2012), 21–13 March, “leukocyte development and function in vivo—studies in zebrafish”.

Karlsruhe, Germany Invited speaker

ZDM5 “Fishing for answers: zebrafish models of human development and disease” (5th Zebrafish Disease Modelling Workshop, a Cold Spring Harbor Asia meeting), 16–20 April, “learning about leukocytes in inflammation and infection using zebrafish models”.

Suzhou, China Invited speaker

10th International Conference on Zebrafish Development and Genetics, 20–24 June, Hematology and Immunology Concurrent Session.

Madison, Wisconsin, uSA

Chairperson

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Julian Heng BRIMS neuroscience Symposium 2012

Kuala lumpur, Malaysia Invited speaker

the Kunming Institute of Zoology Kunming, China Invited speaker

ComBio 2012 Adelaide, South Australia

Invited plenary speaker

Fifth Melbourne Cell and Developmental Biology meeting of the Australia and new Zealand Society for Cell and Developmental Biology

Melbourne, Victoria Conference convenor

Gordon Research Conference on neural Development

Rhode Island, uSA Invited speaker

Department of Surgery, the university of Melbourne

Melbourne, Victoria Invited presentation

32nd Annual Meeting of the Australian neuroscience Society

Gold Coast, Queensland Speaker

31st Australian neuroscience Society meeting

Gold Coast, Queensland Invited speaker

James Bourne

Regenerative Medicine, university of Western Australia

Perth, Western Australia Invited speaker

Monash Biomedical Imaging opening Symposium

Monash university, Melbourne, Victoria

Invited speaker

Queensland Brain Institute Brisbane, Queensland Invited speaker

Baker IDI Institute Melbourne, Victoria Invited speaker

Monash Medical Centre Clinical neurology

Melbourne, Victoria Invited speaker

teAM VIC Regenerative Medicine Symposium

Monash university, Melbourne, Victoria

Invited speaker

nation trauma Research Institute—Plenary Speaker at national Symposium

Melbourne, Victoria Invited speaker

Marmoset Research International (Sfn Satellite)

new orleans, uSA Invited speaker

Japanese Society for Medical Research—Speaker at annual meeting

tokyo, Japan Invited speaker

Second Indian ocean Rim Muscle Colloquium, February 6–7

Bangalore, India Invited speaker

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Peter Currie “Frontiers in Muscle Biology” sponsored by the International Society for Muscle Biology

new York, uSA Invited speaker

5th Annual Zebrafish Disease Models Meeting Fishing for Answers: Zebrafish Models of Human Development and Disease, 16–20 April, Cold Spring Harbor Asia Conference

Suzhou, China Invited speaker

Australian and new Zealand Society of Cell and Developmental Biology

nSW Keynote speaker

Centre for Cancer Biology South Australia Invited speaker

Southern Health Research Week Melbourne, Victoria Keynote speaker

Infrafrontier—european mouse resources

Germany Plenary speaker

nadia Rosenthal

Keystone Cardiovascular Development and Regeneration Symposium

taos, new Mexico Plenary speaker

everett Symposium, Charleston Charleston, uSA Keynote speaker

3rd Australasian Wound & tissue Repair Society Meeting, “Repair and Regeneration”, 22–24 May

Sydney, nSW Keynote speaker

9th World Biomaterials congress Chengdu, China Plenary speaker

Australia-China Symposium on Healthy Ageing, 22–24 July

Canberra, ACt Invited speaker

Joint meeting of the International Society of Differentiation and the netherlands Institute of Regeneration, 5–8 november

Amsterdam, the netherlands

Invited speaker

Creative Innovation 2012 Melbourne, Victoria Keynote speaker

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conferences and seminars (cont)

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students

higher degree by research (phd) students

PhD Student Supervisor Project Scholarship

Bianca Borchin tiziano Barberi Derivation of skeletal muscle progenitors from human embryonic stem cells

Australian Rotary Health Scholarships

leon teo James Bourne novel conjugated biomaterials in the treatment of neurotrauma and neurodegeneration

SMARt Scholarship and ARMI Department Scholarship

ophelia ehrlich Peter Currie nanomedicine based therapies for extracellular matrix diseases

SMARt Scholarship

Zhenhua li Peter Currie Investigating muscle regeneration in zebrafi sh muscular dystrophy mutants

Monash Graduate Scholarship and Monash International Postgraduate Research Scholarship

Wouter Masselink

Peter Currie Cellular interplay of pectoral fi n myogenic progenitor cells

ARMI Department Scholarship

Phong nguyen Peter Currie origins and genetic control of progenitor and stem cells from zebrafi sh

Australian Postgraduate Award

Ivan ng Julian Heng Genetic analysis of neocortical development in mice


Sultan Alasmari

Graham lieschke

Studies of neutrophil behaviour and function in vivo

Sponsored by the Saudi Arabian Cultural Mission

Vahid Pazhakh Graham lieschke

Regulation of leukocyte behaviour during infl ammation in vivo


eamon Coughlan

edwina McGlinn miR-196 in the development of the CnS

Australian Postgraduate Award

Daniel Sieiro Mosti

Christophe Marcelle

Molecular pathways involved in vertebrate myoblast fusion

Faculty of Medicine International Postgraduate Research Scholarship

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honours students

Honours Student Supervisor Project

Skye Kinder James Bourne the role of ephrins A2 and A5 in the development of the mammalian visual cortex

linh ngo Julian Heng the role of the cytoskeleton in the pathogenesis of microcephaly and mental retardation

Jeremy ng Christophe Marcelle expression Analysis and Functional Study of Candidate Genes for Muscle Fusion

tegan Strait nadia Rosenthal (Milena Furtado)

understanding cardiac regeneration in mammals

Joelle Perera nadia Rosenthal (Mauro Da Costa)

Developing Mouse Models for nkx2-5 derived dilated cardiomyopathy

Ryan Debuque nadia Rosenthal (Mauro Da Costa)

Generation of MeS cells as a platform for the study of transcriptional networks and signalling events in cardiomyocyte specification, homeostasis and regeneration.

undergraduate research opportunities program (urop) studentsARMI hosted six undergraduate Research opportunities Program (uRoP) students in 2012, three of which went on to pursue Honours (one at ARMI) and all of which were excellent students.

uRoP provides high calibre undergraduate students with an opportunity to gain experience in a research environment. the selection process is highly competitive and prospective students are matched to specific projects/supervisors. the program is run through the Bio21 Cluster. Bio21 Cluster, a not-for-profit public company, is the governing body of a collaborative cluster of 22 Melbourne institutions, encompassing universities, tertiary health services, medical research institutes, CSIRo and other member-based organisations. the program is a paid employment scheme and is open to any undergraduate student enrolled in a Victorian tertiary institution.

UROP Student Group Supervisor Project

Andrew Giang Currie Patricia Jusuf Genetic control of generating visual nerve cells during development

Ibrahim Yousef Currie Yona Goldshmit Pro-regenerative mechanisms involve in zebrafish spinal cord regeneration.

Caroline Roberts Heng Julian Heng understanding the genetic mechanisms for cell migration during brain development

Daniel Colquhoun

Kaslin Jan Kaslin the role of HMG proteins and splicing factors in developmental neurogenesis and adult regeneration

emily Scott lieschke Cristina Keightley Investigation of ZBtB11 protein-protein interactions.

Mark Sedrak Marcelle David Salgado Compare data management system and application creation

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undergraduate students

Undergraduate Student Research Group Program

Anthony Boghdadi Bourne Summer Vacation Scholarship / Research in Action

Dylan Fox Bourne Summer Vacation Scholarship

Chloe Hammond Bourne Research in Action

Gopika Krishnamurthy Bourne Winter Vacation Scholarship / Research in Action

Stephanie Houston Heng Winter Vacation Scholarship / Research in Action

Sophia Mah Heng Winter Vacation Scholarship

Felicity Barnes Hobbs Summer Vacation Scholarship

Daniel Colquhoun Kaslin Research in Action

Ismath elias Marcelle Research in Action

John Jordan McGlinn Research in Action

Glenda Caruso McGlinn Research in Action

Shuk-Yin tsang McGlinn Research in Action

Alice Wookey Rosenthal Research in Action

International visiting students

Visiting Student Institute of Origin Group Project title

Denis Azabdaftari university of Applied Sciences Berlin, Germany

lieschke new transgenic tools for studying infl ammation in zebrafi sh

Johannes Wittig university of Applied Science HS-lausitz, Germany

lieschke transgenic zebrafi sh for studying leukocytes

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postdoctoral research staff

Postdoctoral Researcher Supervisor Project Fellowship

Yona Goldshmit Peter Currie Mechanisms involved in central nervous system injuries

Joachim Berger Peter Currie Modelling human myopathies in zebrafish

Catherine Boisvert Peter Currie Skeletal evolution in early gnathostomes Human Frontiers

Patricia Jusuf Peter Currie Developmental fate determination of neural subtypes in the zebrafish retina

nHMRC CJ Martin fellowship

Ashley Siegel Peter Currie Generating a targeted mutation resource in zebrafish

ARC linkage project

Ivana Mirkovic Peter Currie Role of the scube gene family during vertebrate muscle development

James Godwin nadia Rosenthal the immunological regulation of salamander regeneration

Alex Pinto nadia Rosenthal Biology of macrophages in the injured and uninjured mammalian health

Mauro Da Costa nadia Rosenthal Role of the cardiac transcription factors in homeostasis and disease

Milena Furtado nadia Rosenthal understanding the role of cardiac transcriptional networks in the non-myocardial component in homeostasis and regeneration

ekaterina Salimova

nadia Rosenthal Dissecting roles of pro-regenerative factors IGF-1 and Relaxin in promoting cardiac repair and regeneration

Stem Cells Australia

lina Wang nadia Rosenthal Differentiation of cardiomyocytes and skeletal myocytes from embryonic stem cells

Stem Cells Australia

olivier Serralbo Christophe Marcelle

Genetic control of the myoblast differentiation

David Salgado Christophe Marcelle

Compare/Myobase multiorganism database for muscle biology and muscular disease

Frederico Calhabeu

Christophe Marcelle

Characterisation of muscle progenitor populations during embryogenesis

Claire Hirst Christophe Marcelle

the generation and use of reporters to delineate the molecular pathways involved in myogenesis

Felix ellett Graham lieschke Analysis of leukocyte behaviours in response to P. marneffei infection

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Postdoctoral Researcher Supervisor Project Fellowship

Cristina Keightley Graham lieschke Investigating the roles of novel transcription factor, Zbtb11, in myelopoiesis and liver development in health and disease

Chieh-Huei Wang Graham lieschke Study of leukocyte response to redox signals during infl ammation

Isabella Mengarelli tiziano Barberi Generation of neural crest cells from human eS Cells

Joly Kwek tiziano Barberi Role of prominin-1 in human embryonic stem cell differentiation

Jihane Homman-ludiye

James Bourne Molecular patterning of the primate visual cortex.

Gurpreet Kaur nicolas Plachta transcription factor diffusion dynamics in the developing mouse embryo

Juan Carlos Fierro-Gonzalez

nicholas Plachta Combining single-cell imaging and quantitative methods to study the molecular mechanisms regulating the adaptive capacity of cells in living mammalian embryos.

Wenner-Gren Foundations (WGS), and Swedish Society for Medical Research (SSMF) Postdoctoral Fellowships

Melanie White nicholas Plachta Imaging the early events patterning mammalian embryos

Alysha Heimberg edwina McGlinn the evolution of Hox-embedded microRnA genes and the animal body plan

Jesus Casanova edwina McGlinn microRnA control of vertebral number and identity

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BioeYeS Australia is an innovative, hands-on approach to teaching primary and secondary students about developmental biology, stem cells and regeneration. over the course of a week, students watch the transparent eggs of zebrafish change from a single-celled zygote to a larval fish. through school incursions facilitated by university lecturers and researchers, students study the lifespan and development of zebrafish anatomy, habitat and genetics.

bioeyes mission • Excite children about the thrill of scientific

discovery• Encourage students to develop their critical

thinking skills, perform • Collaborate on experiments with classmates

studying anatomy, circulation, respiration, genetics and habitat

• Enhance research excellence by building linkages with research users and providers against the backdrop of Monash stem cell science and biomedical research, enhancing international collaborative endeavours

• Capture the inherent enthusiasm and excitement that students have for science; open their eyes to possible and fulfilling futures in research

this year Bioeyes has continued to reach students from schools across the greater Melbourne metropolitan area. Additional schools are signing up as we go to press.

In 2011, the Department of Innovation, Industry, Science and Research (DIISR) provided a small grant to BioeYeS which was critical in seeking further funding, in particular, from Schools Connect, an independent organisation focused on facilitating partnerships between education, business and philanthropy. BioeYeS Australia was selected as one of five recipients for funding from Schools Connect.

With additional funding, we have been able to employ Anmei Vuong as our primary outreach educator. Anmei has been an invaluable participant in the program and works closely with both students and teachers on delivery of BioeYeS.

Further information about BioeYeS Australia including booking an event for primary school students is available from Sharon Flecknoe at [email protected].

BioeYeS outreach Program

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1. Asharani, P.V., K. Keupp, o. Semler, W. Wang, Y. li, H. thiele, G. Yigit, e. Pohl, J. Becker, P. Frommolt, C. Sonntag, J. Altmuller, K. Zimmermann, D.S. Greenspan, n.A. Akarsu, C. netzer, e. Schonau, R. Wirth, M. Hammerschmidt, P. nurnberg, B. Wollnik, and t.J. Carney, Attenuated BMP1 function compromises osteogenesis, leading to bone fragility in humans and zebrafi sh. Am J Hum Genet, 2012. 90(4): 661-674 Category: Genetics & Heredity Impact Factor: 10.603 Ranking: 8/157

2. Benard, e.l., A.M. van der Sar, F. Ellett, G.J. Lieschke, H.P. Spaink, and A.H. Meijer, VIDeo: Infection of zebrafi sh embryos with intracellular bacterial pathogens. J Vis exp, 2012. 61(e3781): 1-8

3. Berger, J. and P.D. Currie, Zebrafi sh models fl ex their muscles to shed light on muscular dystrophies. Dis Model Mech, 2012. 5(6): 726-732 Category: Cell Biology Impact Factor: 4.937 Ranking: 54/181

4. Berger, J., T. Sztal, and P.D. Currie, Quantifi cation of birefringence readily measures the level of muscle damage in zebrafi sh. Biochem Biophys Res Commun, 2012. 423(4): 785-788 Category: Biochemistry & Molecular Biology Impact Factor: 2.484 Ranking: 173/289

5. Bogoyevitch, M.A, Y.Y. Yeap, Z. Qu, K.R. ngoei, Y.Y. Yip, t.t. Zhao, J.I. Heng, and D.C. ng, WD40-repeat protein 62is a JnK-phosphorylated spindle pole protein required for spindle maintenance and timely mitotic progression. J Cell Sci, 2012. nov 1;125(Pt 21): 5096-109

6. Breuss, M., J.I. Heng, K. Poirier, G. tian, X.H. Jaglin, Z. Qu, A. Braun, t. Gstrein, l. ngo, M. Haas, n. Bahi-Buisson, M.l. Moutard, S. Passemard, A. Verloes, P. Gressens, Y. Xie, K.J. Robson, D.S. Rani, K. thangaraj, t. Clausen, J. Chelly, n.J. Cowan, and D.A. Keays, Mutations in the Ð-tubulin gene tuBB5 cause microcephaly with structural brain abnormalities. Cell Rep, 2012. Dec 27;2(6):1554-62

7. Bryson-Richardson, R.J., S. Berger, and P.D. Currie, Atlas of Zebrafi sh Development. 1st ed, ed. R. Bryson-Richardson. Vol. 1. 2012, uK: Academic Press. 238 [ARMI,SOBS]

8. Cai, H., F.S. Santiago, l. Prado-lourenco, B. Wang, M. Patrikakis, M.P. Davenport, G.J. Maghzal, R. Stocker, C.R. Parish, B.H. Chong, G.J. Lieschke, t.W. Wong, C.n. Chesterman, D.J. Francis, F.J. Moloney, R.S. Barnetson, G.M. Halliday, and l.M. Khachigian, DnAzyme targeting c-jun Suppresses Skin Cancer Growth. Sci transl Med, 2012. 4(139): 139ra82.1-12 Category: Cell Biology Impact Factor: 7.804 ranking: 26/180

9. Chandras, C., M. Zouberakis, e. Salimova, D. Smedley, n. Rosenthal, and V. Aidinis, CreZoo--the european virtual repository of Cre and other targeted conditional driver strains. Database (oxford), 2012. 2012(bas029.): 1-5 Category: Mathemathics & Computational Biology Impact Factor: 2.071 Ranking: 15/47

10. da Silva, M.G., e. Mattos, J. Camacho-Pereira, t. Domitrovic, A. Galina, M.W. [da] Costa, and e. Kurtenbach, Cardiac systolic dysfunction in doxorubicin-challenged rats is associated with upregulation of MuRF2 and MuRF3 ligases. exp Clin Card, 2012. 17(3): 101-109 Category: Cardiac & Cardiovascular Systems Impact Factor: 0.576 Ranking: 109/117

11. Dworkin, S., C. Darido, S.R. Georgy, t. Wilanowski, S. Srivastava, F. Ellett, L. Pase, Y. Han, A. Meng, J.K. Heath, G.J. lieschke, and S.M. Jane, Midbrain-hindbrain boundary patterning and morphogenesis are regulated by diverse grainy head-like 2-dependent pathways. Development, 2012. 139(3): 525-536 Category: Developmental Biology Impact Factor: 6.596 Ranking: 5/40

12. Ellett, F. and G.J. Lieschke, Computational Quantifi cation of fl uorescent leukocyte numbers in Zebrafi sh embryos, in Methods in enzymology – Imaging and Spectroscopic Analysis of living CellsImaging live Cells in Health and Disease, P. Conn, editor. 2012, Academic Press: uSA. 425-435 Book Chapter

Publications

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13. Frisca, F., R.A. Sabbadini, Y. Goldshmit, and A. Pebay, Biological effects of lysophosphatidic acid in the nervous system. Int Rev Cell Mol Biol, 2012. 296: 273-322 Category: Biochemistry & Molecular Biology Impact Factor: 4.481 Ranking: 70/289

14. Frugier, t., A. Conquest, C. Mclean, P. Currie, D. Moses, and Y. Goldshmit, expression and activation of ephA4 in the human brain after traumatic injury. J neuropathol exp neurol, 2012. 71(3): 242-250 Category: Clinical neurology Impact Factor: 4.258 Ranking: 26/191

15. Ganz, J., J. Kaslin, D. Freudenreich, A. Machate, M. Geffarth, and M. Brand, Subdivisions of the adult zebrafish subpallium by molecular marker analysis. J Comp neurol, 2012. 520(3):633-55

16. Garcia-Cao, I., M.S. Song, R.M. Hobbs, G. laurent, C. Giorgi, V.C. de Boer, D. Anastasiou, K. Ito, A.t. Sasaki, l. Rameh, A. Carracedo, M.G. Vander Heiden, l.C. Cantley, P. Pinton, M.C. Haigis, and P.P. Pandolfi, Systemic elevation of Pten induces a tumor-suppressive metabolic state. Cell, 2012. 149(1): 49-62 Category: Biochemistry & Molecular Biology Impact Factor: 32.403 Ranking: 2/290

17. Garcia-Moreno, F., n.A. Vasistha, n. trevia, J.A. Bourne, and Z. Molnar, Compartmentalization of cerebral cortical germinal zones in a lissencephalic primate and gyrencephalic rodent. Cereb Cortex, 2012. 22(2): 482-492 Category: neurosciences Impact Factor: 6.544 Ranking: 22/243

18. Goldshmit, Y., R. Matteo, T. Sztal, F. Ellett, F. Frisca, K. Moreno, D. Crombie, G.J. Lieschke, P.D. Currie, R.A. Sabbadini, and A. Pebay, Blockage of lysophosphatidic Acid Signaling Improves Spinal Cord Injury outcomes. Am J Pathol, 2012. 181(3): 978-992 Category: Pathology Impact Factor: 4.890 Ranking: 6/78

19. Goldshmit, Y., T.E. Sztal, P.R. Jusuf, T.E. Hall, M. Nguyen-Chi, and P.D. Currie, Fgf-dependent glial cell bridges facilitate spinal cord regeneration in zebrafish. J neurosci, 2012. 32(22): 7477-7492 Category: neurosciences Impact Factor: 7.115 Ranking: 19/243

20. Hansson, J., M. Reza Rafiee, S. Reiland, J.M. Polo, J. Gehring, S. okawa, W. Huber, K. Hochedlinger, and J. Krijgsveld, Highly coordinated proteome dynamics during reprogramming of somatic cells to pluripotency, Cell Reports, 2012. 2(6):1579-1592

21. Hede, M.S., E. Salimova, A. Piszczek, e. Perlas, n. Winn, t. nastasi, and N. Rosenthal, e-peptides control bioavailability of IGF-1. PloS one, 2012. 7(12): e51152.1-11 Category: Biology Impact Factor: 4.092 Ranking: 12/85

22. Heimberg, A. and E. McGlinn, Building a robust A-P Axis. Curr Genomics, 2012. 13(4): 278-288 Category: Genetics & Heredity Impact Factor: 2.408 Ranking: 89/158

23. Hobbs, R.M., S. Fagoonee, A. Papa, K. Webster, F. Altruda, R. nishinakamura, l. Chai, and P.P. Pandolfi, Functional antagonism between Sall4 and Plzf defines germline progenitors. Cell Stem Cell, 2012. 10(3): 284-298 Category: Cell Biology Impact Factor: 25.421 Ranking: 4/181 [ARMI,MISCL]

24. Hochmann, S., J. Kaslin, S. Hans, A. Weber, A. Machate, M. Geffarth, R.H. Funk, and M. Brand, Fgf signaling is required for photoreceptor maintenance in the adult zebrafish retina. PloS one, 2012. 7(1): e30365.1-12 Category: Biology Impact Factor: 4.092 Ranking: 12/85

25. Homman-Ludiye, J., T.D. Merson, and J.A. Bourne, the early postnatal nonhuman primate neocortex contains self-renewing multipotent neural progenitor cells. PloS one, 2012. 7(3): e34383 Category: Biology Impact Factor: 4.092 Ranking: 12/85

26. Hu, J.K., E. McGlinn, B.D. Harfe, G. Kardon, and C.J. tabin, Autonomous and nonautonomous roles of Hedgehog signaling in regulating limb muscle formation. Genes Dev, 2012. 26(18): 2088-2102 Category: Cell Biology Impact Factor: 11.659 Ranking: 3/40

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27. Johnson, J.l., T.E. Hall, J.M. Dyson, C. Sonntag, K. Ayers, S. Berger, P. Gautier, C. Mitchell, G.e. Hollway, and P.D. Currie, Scube activity is necessary for Hedgehog signal transduction in vivo. Dev Biol, 2012. 368(2): 193-202 Category: Developmental Biology Impact Factor: 4.069 Ranking: 7/40 [ARMI,BCH]

28. Jusuf, P.R., S. Albadri, A. Paolini, P.D. Currie, F. Argenton, S. Higashijima, W.A. Harris, and l. Poggi, Biasing amacrine subtypes in the Atoh7 lineage through expression of Barhl2. J neurosci, 2012. 32(40): 13929-13944 Category: neurosciences Impact Factor: 7.115 Ranking: 19/244

29. Kaslin, J. and M. Brand, Cerebellar development and neurogenesis in zebrafi sh. In Handbook of the Cerebellum and Cerebellar Disorders, M. Manto, D. Gruol, J. Schmahmann, n. Koibuchi, and F. Rossi, editors, 2012, Springer: new York, Berlin, Heidelberg

30. Kizil, C.#, J. Kaslin#, V. Kroehne, and M. Brand, Adult neurogenesis and brain regeneration in zebrafi sh. Dev neurobiol, 2012. Mar;72(3):429-61 (# these authors contributed equally to the work)

31. Kizil, C., n. Kyritsis, S. Dudczig, V. Kroehne, D. Freudenreich, J. Kaslin, and M. Brand, Regenerative neurogenesis from neural Progenitor Cells Requires Injury-Induced expression of Gata3. Dev Cell, 2012. 23: 1-8 Category: Cell Biology Impact Factor: 14.030 Ranking: 9/181

32. Krallinger, M., F. leitner, M. Vazquez, D. Salgado, C. Marcelle, M. tyers, A. Valencia, and A. Chatr-aryamontri, How to link ontologies and protein-protein interactions to literature: text-mining approaches and the BioCreative experience. Database (oxford), 2012. 2012(Article bas017): 1-12 Category: Mathematical & Computational Biology Impact Factor: 2.071 Ranking: 15/47

33. Kyritsis, n., C. Kizil, S. Zocher, V. Kroehne, J. Kaslin, D. Freudenreich, A. Iltzsche, and M. Brand, RePoRt: Acute Infl ammation Initiates the Regenerative Response in the Adult Zebrafi sh Brain. Science, 2012. 338(6112): 1353-1356 Category: Multidisciplinary Sciences Impact Factor: 31.201 Ranking: 2/56

34. lam, n.t., P.D. Currie, G.J. Lieschke, N.A. Rosenthal, and D.M. Kaye, nerve growth factor stimulates cardiac regeneration via cardiomyocyte proliferation in experimental heart failure. PloS one, 2012. 7(12): e53210.1-9 Category: Biology Impact Factor: 4.092 Ranking: 12/85

35. Lui, L.L., A.E. Dobiecki, J.A. Bourne, and M.G. Rosa, Breaking camoufl age: responses of neurons in the middle temporal area to stimuli defi ned by coherent motion. eur J neurosci, 2012. 36(1): 2063-2076 Category: neurosciences Impact Factor: 3.631 Ranking: 80/243 [ARMI,PHY]

36. Macheda, M.l., W.W. Sun, K. Kugathasan, B.M. Hogan, n.I. Bower, M.M. Halford, Y.F. Zhang, B.e. Jacques, G.J. Lieschke, A. Dabdoub, and S.A. Stacker, the Wnt Receptor Ryk Plays a Role in Mammalian Planar Cell Polarity Signaling. J Biol Chem, 2012. 287(35): 29312-29323 Category: Biochemistry & Molecular Biology Impact Factor: 4.773 Ranking: 66/289

37. Mansfi eld, J.H. and E. McGlinn, evolution, expression, and developmental function of Hox-embedded miRnAs, in Curr top Dev Biol, PM Wasserman and o. Pourquie, editors. 2012, Academic Press: uSA. 31-57 Category: Developmental Biology Impact Factor: 6.000 Ranking: 6/40

38. Martin, K.R., A. Corlett, D. Dubach, t. Mustafa, H.A. Coleman, H.C. Parkington, t.D. Merson, J.A. Bourne, S. Porta, M.l. Arbones, D.I. Finkelstein, and M.A. Pritchard, over-expression of RCAn1 causes Down syndrome-like hippocampal defi cits that alter learning and memory. Hum Mol Genet, 2012. 21(3): 3025-3041 Category: Genetics & Heredity Impact Factor: 7.636 Ranking: 13/157 [BCH,PHY,ARMI]

39. Nguyen-Chi, M.E., R. Bryson-Richardson, C. Sonntag, T.E. Hall, A. Gibson, T. Sztal, W. Chua, t.F. Schilling, and P.D. Currie, Morphogenesis and Cell Fate Determination within the Adaxial Cell equivalence Group of the Zebrafi sh Myotome. PloS Genet, 2012. 8(10): e1003014.1-16 Category: Genetics & Heredity Impact Factor: 8.694 Ranking: 11/158

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40. nicholls, C., A.R. Pinto, H. li, l. li, l. Wang, R. Simpson, and J.P. liu, Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) induces cancer cell senescence by interacting with telomerase RnA component. Proc natl Acad Sci u S A, 2012. 109(33): 13308-13313 Category: Multidisciplinary Sciences Impact Factor: 9.681 Ranking: 3/55

41. Pase, L., J.e. layton, C. Wittmann, F. Ellett, C.J. nowell, C.C. Reyes-Aldasoro, S. Varma, K.l. Rogers, C.J. Hall, M.C. Keightley, P.S. Crosier, C. Grabher, J.K. Heath, S.A. Renshaw, and G.J. Lieschke, neutrophil-delivered myeloperoxidase dampens the hydrogen peroxide burst after tissue wounding in zebrafish. Curr Biol, 2012. 22(19): 1818-1824 Category: Biochemistry & Molecular Biology Impact Factor: 9.647 Ranking: 18/290

42. Pase, L., C.J. nowell, and G.J. Lieschke, In Vivo Real-time Visualization of leukocytes and Intracellular Hydrogen Peroxide levels During a Zebrafish Acute Inflammation Assay, in Methods in enzymology – Imaging and Spectroscopic Analysis of living CellsImaging live Cells in Health and Disease, P. Conn, editor. 2012, Academic Press: uSA. 135-156 Book Chapter

43. Pinto, A.R., R. Paolicelli, e. Salimova, J. Gospocic, e. Slonimsky, D. Bilbao-Cortes, J.W. Godwin, and N.A. Rosenthal, An abundant tissue macrophage population in the adult murine heart with a distinct alternatively-activated macrophage profile. PloS one, 2012. 7(5): e36814.1-11 Category: Biology Impact Factor: 4.092 Ranking: 12/84

44. Polo, J.M., e. Anderssen, R.M. Walsh, B.A. Schwarz, C.M. Nefzger, S.M. Lim, M. Borkent, e. Apostolou, S. Alaei, J. Cloutier, o. Bar-nur, S. Cheloufi, M. Stadtfeld, M.e. Figueroa, D. Robinton, S. natesan, A. Melnick, J. Zhu, S. Ramaswamy, and K. Hochedlinger, A Molecular Roadmap of Reprogramming Somatic Cells into iPS Cells. Cell, 2012. 151(7): 1617-1632 Category: Biochemistry & Molecular Biology Impact Factor: 32.403 Ranking: 2/290 [MISCl,ARMI]

45. Rios, A.C. and C. Marcelle, A tale of stolen kiss in muscle. Med Sci (Paris), 2012. 28(3): 264-266 Category: Medicine, Research & experimental Impact Factor: 0.516 Ranking: 94/111

46. Rios, A.C., C. Marcelle, and O. Serralbo, Gene loss-of-function and live imaging in chick embryos, in Methods Mol Biol, K. turksen, editor. 2012, Humana Press: uSA. 105-117

47. Rosenthal, N. and C. Stewart, eDItoRIAl: In search of a wide-angle perspective. Differentiation, 2012. 83(3): iii-iv (3-4) Category: Cell Biology Impact Factor: 2.807 Ranking: 106/180

48. Ruparelia, A.A., M. Zhao, P.D. Currie, and R.J. Bryson-Richardson, Characterization and Investigation of zebrafish models of Filamin related myofibrillar myopathy. Hum Mol Genet, 2012. 21(18): 4073-4083 Category: Biochemistry & Molecular Biology Impact Factor: 7.636 Ranking: 31/289

49. Salgado, D., M. Krallinger, M. Depaule, e. Drula, A. tendulkar, F. leitner, A. Valencia, and C. Marcelle, MyMiner: a web application for computer-assisted biocuration and text annotation. Bioinformatics, 2012. 28(17): 2285-2287 Category: Mathematical & Computational Biology Impact Factor: 5.468 Ranking: 1/47

50. Salgado, D., C. Marcelle, P.D. Currie, and R.J. Bryson-Richardson, the Zebrafish Anatomy Portal: A novel integrated resource to facilitate zebrafish research. Dev Biol, 2012. 372(1): 1-4 Category: Developmental Biology Impact Factor: 4.069 Ranking: 7/40

51. Santini, M.P. and N. Rosenthal, Myocardial Regenerative Properties of Macrophage Populations and Stem Cells. J Cardiovasc transl Res, 2012. 5(5): 700-712 Category: Cardiac & Cardiovascular Systems Impact Factor: 2.611 Ranking: 45/117

52. Sztal, T., H. Chung, S. Berger, P.D. Currie, P. Batterham, and P.J. Daborn, A cytochrome p450 conserved in insects is involved in cuticle formation. PloS one, 2012. 7(5): e36544.1-9 Category: Biology Impact Factor: 4.092 Ranking: 12/84

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53. Sztal, T.E., C. Sonntag, T.E. Hall, and P.D. Currie, epistatic dissection of laminin-receptor interactions in dystrophic zebrafi sh muscle. Hum Mol Genet, 2012. 21(21): 4718-4731 Category: Biochemistry & Molecular Biology Impact Factor: 7.636 Ranking: 31/289

54. Teo, L., J. Homman-Ludiye, J. Rodger, and J.A. Bourne, Discrete ephrin-B1 expression by specifi c layers of the primate retinogeniculostriate system continues throughout postnatal and adult life. J Comp neurol, 2012. 520(13): 2941-2956 Category: neurosciences Impact Factor: 3.808 Ranking: 69/243

55. Teo, L., J.V. Rosenfeld, and J.A. Bourne, ReVIeW: Models of CnS injury in the nonhuman primate: a new era for treatment strategies. trans neuroscience, 2012. 3(2): 181-195

56. touvron, M., B. escoubet, M. Mericskay, A. Angelini, l. lamotte, M.P. Santini, N. Rosenthal, D. Daegelen, D. tuil, and J.F. Decaux, locally expressed IGF1 propeptide improves mouse heart function in induced dilated cardiomyopathy by blocking myocardial fi brosis and SRF-dependent CtGF induction. Dis Model Mech, 2012. 5(4): 481-491 Category: Cell Biology Impact Factor: 4.937 Ranking: 54/180

57. Warner, C.E., W.C. Kwan, and J.A. Bourne, the early Maturation of Visual Cortical Area Mt is Dependent on Input from the Retinorecipient Medial Portion of the Inferior Pulvinar. J neurosci, 2012. 32(48): 17073-17085 Category: neurosciences Impact Factor: 7.115 Ranking: 19/244

58. Wittmann, C., P. Chockley, S.K. Singh, L. Pase, G.J. Lieschke, and C. Grabher, ReVIeW: Hydrogen peroxide in infl ammation: messenger, guide, and assassin. Adv Hematol, 2012. 2012: 541471.1-6

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How to donate to ARMI

ARMI researches the basic mechanisms behind the process of regeneration, seeking to find effective treatments for a range of diseases. Your donation can help fund our research projects.

Donors can choose to support the Australian Regenerative Medicine Institute in a variety of ways:

1. For an online gifts please go to https://community.monash.edu.au/givetoarmi

2. If it is more convenient to donate by cheque or money order, donations can be mailed to:Monash university AdvancementPo Box 197 Caulfield east, VIC 3145 Australia

Cheques should be made payable to “Monash university”. You will also need to provide your name, contact details, gift amount, whether you wish to remain anonymous and instructions to direct the gift to ARMI.

3. Direct deposit You can make a direct deposit into Monash university’s bank account. Please contact Monash universty on +61 3 9903 1608 to get the relevant details. once you make the donation, your remittance advice should be emailed to us. Please tell us your name, the donation amount and when the donation was made.

4. Make a gift by phone Credit card donations including single gifts and on-going pledges can be made by calling our Donor Stewardship officer, Anne Chartres, on +61 3 9903 1608.

Please note that we are unable to take direct debits over the phone.

5. Donating by fax Credit card donations can be faxed to our confidential fax number: +61 3 9903 4885. We don’t recommend sending your credit card details by email.

All donations over $2 to the Institute are tax-deductible. A letter and a receipt will acknowledge all donations.

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ARMI Stakeholders Extending internationally 04 01 01 12 31 ... Report_2012... · 6 Page ARMI AnnuAl...

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Transcript of ARMI Stakeholders Extending internationally 04 01 01 12 31 ... Report_2012... · 6 Page ARMI AnnuAl...