AP Biology DiscussionNotes

Friday 01/16/2015

Goals for Today• Be able to describe how restriction

enzymes work and why they are valuable to scientists

• Be able to interpret a restriction map and predict restriction fragments produced by different restriction digests.

• Be able to describe how DNA extraction, gel electrophoresis, & PCR reactions work

Question of the Day 1/16• What is a restriction/recognition site?

Question of the Day 1/16• What is a restriction/recognition site?

•GAATTC

DNA Scissors• Palindromes

–Read the same 5’-3’

• Base pairs must match (be complimentary)!

DNA Assignment

• Turn in IF 100% Complete

• DO NOT WORK ON THIS IN CLASS – the time has passed – need to work on our new assignments

Virtual Labs

• DNA Extraction

• Gel Electrophoresis

• PCR (Polymerase Chain Reactions)

Work Order Today

• Work on finishing DNA scissors

• Work on Biotech Virtual Labs–Take your time to READ carefully!–These topics will become fair game

for a quiz! –ASK QUESTIONS

Planning Ahead…

• Monday -DNA Scissors DUE• Friday – Quiz on DNA structure/replication

& Names!

• Next Discussion:– Read through chapter 17 (page 122)

Species of the Day 1/16• Downy Woodpecker

– Picoides pubescens

Species of the Day 1/16• Downy Woodpecker

– Picoides pubescens

Species of the Day 1/16• Downy Woodpecker

• What enzyme is this woodpecker is responsible for unzipping the Double helix so replication can begin?