CHBE 571 Flow and Transport in Porous Media I Geology, Chemistry and Physics of Fluid Transport
Animal Science 434 Lecture 15: Gamete Transport. Sperm Transport in the Male Tract Fluid Flow Cilia...
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Transcript of Animal Science 434 Lecture 15: Gamete Transport. Sperm Transport in the Male Tract Fluid Flow Cilia...
Sperm Transport in the Male Tract
• No protein synthetic ability• addition or loss of plasma membrane proteins and lipids• redistribution of proteins and lipids within sperm• changes in lipid diffusion coefficients
Acquisition ofFertilizing Ability
and Motility
Sperm Transport in the Male Tract
Addition of Decapacitation
Factor Addition of DF From Accessory Gland Secretions
Decapacitation Factor• protein, glycolipid and/or lipid• stabilizes the plasma membrane
Site of Ejaculation
Site of ejaculation Semen Characteristics Species
Vagina slight coagulation of ejaculate human, rabbit
vagina semen with high sperm cattle, sheepconcentration
uterus voluminous, distention of cervix horse
cervix, uterus voluminous, retention of penis dog, pigduring copulation
uterus vagina plug rodents
Experiment on Retrograde Flow in the Cow
Uterine Horn
Uterine Body
Cervix
Insemination at 3 different
spots
0
5
10
15
20C
um
ula
tiv
e %
Sp
erm
R
ec
ov
ere
d F
rom
Va
gin
a
0 1 2 3 4 5 6 7 8
Hour After Insemination
Uterine Body
Uterine Horn
Cu
mu
lati
ve %
Sp
erm
Rec
ove
red
Fro
m t
he
Vag
ina
0
10
20
30
40
50
60
cervix
0 1 2 3 4 5 6 7 8
Hour After Insemination
Cu
mu
lati
ve %
Sp
erm
Rec
ove
red
Fro
m t
he
Vag
ina
Hours After Insemination
Cervix
Uterine Horn
Evolutionary Strategies for Dealing With Retrograde Flow of Semen
• coagulation of semen or a plug
• concentrated semen
• semen deposited in uterus
Phases of Sperm Transport• rapid
»15-30 min• sustained
»6 - 12 hr• oviductal
»at ovulation
• rapid »15-30 min
• sustained»6 - 12 hr
• oviductal»at ovulation
Effect of Oviduct Transection on Fertilization in Heifers
Interval from mating* to Oocytes Fertilizedtransection of UTJ
6 0%
8 50%
12 83%
* Mating was done at the start of estrus.
Fluid FlowFluid Flow
Factors Regulating Oocyte Transport in the Oviduct
Remains closed untilday 3 - 5 post ovulation
Factors Regulating Oocyte Transport in the Oviduct
Cilia
Fluid FlowFluid FlowMuscleContractions
No Contractions
Remains closed untilday 3 - 5 post ovulation
Site ofFertilization
Site ofFertilization
Oviduct Pick-up of the Oocyte
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