AgraStrip RUR-HS Bulk Grain Y Qualitative Method Strip Test · kernels in 5 minutes, one (1)...

©2013 Romer Labs®, Inc. All Rights Reserved. This document is the property of Romer Labs®, Inc.

AS-GMO-RURHSBULKGRAINTC-7000011-PI-000387-9-GB Finalized 28 July 2014 1

AgraStrip®

RUR-HS Bulk Grain

Strip Test –

Qualitative Method

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Order Number: 7000011

Intended Use

The intended use of the kit is the qualitative (yes/no) determination of the CP4

EPSPS protein in Roundup Ready® bulk seed and grain samples, and alfalfa hay samples. The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait has a detection

limit of one (1) Roundup Ready® (CP4 EPSPS) corn kernel in 800 non-RUR corn

kernels in 5 minutes, one (1) Roundup Ready® canola seed in 1000 non-RUR canola

seeds in 5 minutes, and one (1) Roundup Ready® alfalfa seed in 600 non-RUR

alfalfa seeds in 5 minutes. It has a detection limit of 5% in alfalfa hay (ground or

cored). Note: This application is intended for the detection of NK603

Roundup Ready® corn (RUR NK603). It does not detect genetic event

GA21 Roundup Ready® corn.

Product Description

The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait Test Kit detects the CP4

EPSPS protein produced by a gene, derived from Agrobacterium sp. strain CP4. This

gene has been incorporated into herbicide-resistant crops including Roundup Ready® and Roundup Ready® 2 brands (RUR) from Monsanto and other companies.

The lateral flow strips and other kit components are sufficient to detect the

presence or absence of the CP4 EPSPS protein in both field and laboratory

environments. Different protocols are required for leaf, seed, and bulk grain

detection.

Principle of the Assay

The assay uses a double antibody sandwich format. Antibodies specific to the CP4

EPSPS protein are coupled to a color reagent and incorporated into the lateral flow

strip. When the lateral flow strip is placed in a small amount of an extract from

plant tissue that contains CP4 EPSPS protein, binding occurs between the coupled

antibody and the protein. A sandwich is formed with some, but not all the antibody

that is coupled to the color reagent. The membrane contains two capture zones, one captures the bound CP4 EPSPS protein and the other captures color reagent.

These capture zones display a reddish color when the sandwich and/or unreacted

colored reagents are captured in the specific zones on the membrane. The

presence of only one line (control line) on the membrane indicates a negative

sample and the presence of two lines indicates a positive sample.

Contents of Kit

Quantity

AgraStrip® RUR-HS Bulk Grain Strip Test- Trait 100

Conical microtubes, 1.5mL (COOLS2005) 100

Transfer pipettes (COOLS2010) 100



Romer Labs Methods

Materials Required but not Supplied:

Conical microtube rack (EQOLE1215)

Timer (EQOLE1301) Graduated Cylinder, 50mL (EQOLE1051)

Balance, 400g (EQOLE1010)

Blender (110V: EQOLE1020; 220V: EQOLE1027)

Blender blade with gasket set (EQOLE1026)

Storage and Preparation of Reagents

The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait can be stored at room

temperature or refrigerated (2 -25ºC). Do not freeze. Once opened, the AgraStrip®

RUR-HS Bulk Grain Strip Test - Trait lateral flow test strips must be stored in the

closed canister with the moisture-indicating card. The moisture-indicating card must be

blue in color. If the moisture-indicating card is pink, contact Romer® Labs, Inc.

Technical Service. Storage conditions higher than room temperature may adversely

affect performance.

Purpose

The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait Test Kit has been designed to

screen bulk grain and alfalfa hay samples for the presence of the CP4 EPSPS protein.

Sample preparation procedures are outlined below. Please refer to the instructions to

be sure that all of the necessary materials are available prior to testing.

Sampling

The samples used for the AgraStrip® RUR-HS Bulk Grain Strip Test - Trait Corn Grain

Test Kit can be sub-samples of “representative samples” collected following GIPSA

sampling procedures from trucks, railcars, barges, etc. for other tests. The size of the

sub-samples to be used for the AgraStrip® RUR-HS Bulk Grain Strip Test - Trait will

depend on the percent RUR screening level desired and an acceptable level of risk that

the RUR level is close to the screening level. The number and size of the sub-samples

will be discussed in more detail in the Principle of the Screening Application

section. For canola, the size of the sub-sample used for the AgraStrip® RUR-HS Bulk

Grain Strip Test - Trait has been optimized for 1000 canola seeds. For alfalfa seed,

the size of the sub-sample used for the AgraStrip® RUR-HS Bulk Grain Strip Test -

Trait has been optimized for 600 alfalfa seeds.

Note: It is assumed that the samples collected are representative of the contents of the truck or container and are sufficiently mixed to contain a

random distribution of the sample contents.

Sample Preparation: Weighing the Sample

The statistical sampling plan (see Principle of the Screening Application) is

dependent on the number of seeds or kernels used. However, it is more practical for

routine testing to weigh seeds or kernels instead of counting to obtain the desired

number. The average weight of seeds depends on the variety and environmental

conditions.



Romer Labs Methods

It is recommended that the weight-to-seed/kernel ratio for each variety be determined

as follows:

1. Count 100 kernels of the variety to be tested.

2. Weigh the 100 seeds to the nearest 0.01 gram.

3. Divide the weight of the seeds by 100 to get the average grams per seed.

4. Multiply this average weight by the desired number of seeds in the sub-samples

to determine the weight for the sub-samples.

5. Construct a weight-to-seed ratio table for each variety for the different sub-sample sizes to be used.

Example: One hundred (100) corn kernels of Variety X weigh 25.00 grams. Each corn

kernel then weighs 0.25 grams. Multiply the 0.25-gram per corn kernel times the

number of corn kernels in each sample size to get the following table.

Table A: Example: Weight-to-Seed/Kernel Ratio

Grams per Sample of X

No. Corn Kernels (a) 100 400 600 800

Sample Weight (g) 25 100 150 200

No. Canola Seeds 500 750 1000

Sample Weight (g) 1.75 2.63 3.5

This average weight is then used to obtain the number of corn kernels for this corn

variety.

Sample Preparation

The sample is ground and then extracted with water in a polypropylene “Mason”-type

jar. The sample preparation is important for the proper function of the test, especially

the ratio of water to the weight of the seed sample. The size of “Mason” jar required

and the grinding time depends on the sample size to be analyzed. Table B lists those

parameters.

Corn: Sample Weight (g) x 1.25 = Water Volume (mL)

Canola: Sample Weight (g) x 3.0 = Water Volume (mL) Alfalfa Seed: Sample Weight (g) x 10.0 = Water Volume (mL)

Alfalfa Hay (Ground): Sample Weight (g) x 20.0 = Water Volume (mL)

Alfalfa Hay (Cored): Sample Weight (g) x 15.0 = Water Volume (mL)

Table B: Parameters for Preparing Samples

Number of Kernels in Sample

Jar Size (oz.) Grind Time (sec)

Corn: 25 – 125 4 10 – 20

Corn: 125 – 250 8 15 – 25

Corn: 250 – 500 16 20 – 35

Corn: > 500 32 45 – 60

Canola: 1000 4 10 – 15

Alfalfa Seed: 600 4 10 - 15

The processing parameters were determined using the laboratory grade Waring Model

31BL91 food processor with the standard blade. Other food processors may require

different parameters. All kernels should be ground.



Romer Labs Methods

Sample Processing

1. Weigh sub-samples from each truck or container.

2. Place each sub-sample in a clean, dry polypropylene “Mason” jar of the

appropriate size. See Table B.

3. Attach the jar adapter and clean, dry cutting blades.

4. Place the jar onto the food processor, place a shield over the jar if desired, and

grind the sub-sample on high speed for the time indicated in Table B.

Caution: If using glass “Mason” jars, it is recommended to shield the jars during grinding with a “tri-cornered” 1-liter plastic beaker.

5. Remove the adapter and cutting blades.

Note: A coffee grinder may also be used to grind small sample sizes (i.e.

alfalfa). If a coffee grinder is used, transfer the sample to a clean container

for extraction.

6. Add the volume of water (see below) to the ground seed in the jar, place a lid on

the jar and shake the jar until all the ground corn is well wetted (about 10-20

sec.)

For Corn: Sample Weight (g) X 1.25 = Water Volume (mL)

For Canola: Sample Weight (g) X 3.00 = Water Volume (mL) For Alfalfa Seed: Sample Weight (g) X 10.00 = Water Volume (mL)

For Alfalfa Hay (Ground): Sample Weight (g) X 20.00 = Water Volume (mL)

(i.e. – use 5g/100mL)

For Alfalfa Hay (Cored): Sample Weight (g) X 15.00 = Water Volume (mL)

(i.e. – use 5g/75mL)

Note: The sample will have a “thick” consistency but should contain some free liquid

after a short settling time. There should be no whole seeds remaining.

7. Use this free liquid as sample in the Test Procedure.

Test Procedure

1. Transfer some liquid from the prepared sample to a conical microtube by squeezing the bulb of the transfer pipette and inserting it in the free liquid in the

top of the sample jar.

2. Release the bulb to pull up the sample. Add the sample from the pipette to the

sample tube by squeezing the bulb. This should be approximately 0.5 mL.

3. Place one AgraStrip® RUR-HS Bulk Grain Strip Test - Trait Test Strip into the

sample tube. Let sit for 5 minutes.

4. The appearance of one line (control) on the strip indicates a negative result.

5. The appearance of two lines on the strip indicates a positive result.

The sample tube has a 0.5-mL

indicator at the top of the tapered

section.



Romer Labs Methods

Interpreting the Lateral Flow Strip Test

Check the test strips 5 minutes after inserting the strip. At least one line, the Control

Line, should always develop approximately one (1) cm up from the bottom sample pad.

A red line in this position indicates that the strip is functioning properly. A red line appearing below the Control Line is the RUR Test Line and indicates a positive result for

CP4 EPSPS protein. If the test strip displays two (2) red lines, the test is complete and

the sample is positive for the RUR trait. If at 5 minutes the test strip only shows a

clearly visible Control Line, then the sample is negative for the RUR transgenic trait. If

no control line develops, the result is inconclusive and needs to be repeated.

Note: Test strip results should be interpreted after 5 minutes. Test strips

interpreted after 30 minutes are invalid.

Illustration of Lateral Flow Strips

Archiving Test Strips

If it is desired to archive test strip

results, cut off the bottom and top

strip pads as illustrated to the left

within30 minutes of test completion.

Example of an unreacted, negative (1-

line) and positive (2-lines) test strip



Romer Labs Methods

Equipment Cleaning and Drying

Caution: It is important to clean and dry the jars and cutting blades and/or coffee

grinder between samples.

1. The “Mason” jar should be emptied, rinsed thoroughly with water and completely

dried with a paper towel between uses.

2. The cutting blades for the blender should be rinsed with water until all ground

seed is removed, washed using standard household liquid soap, rinsed well and

carefully dried. If available, spraying or rinsing with methanol or isopropyl (rubbing) alcohol will assist drying.

3. Coffee grinders should be emptied, wiped down with moist towels thoroughly and

completely dried with paper towels between uses. Special attention should be

paid to the area at the base of the blades. It is not advisable to put water directly

into the grinder. Be sure to unplug the grinder when cleaning.

Principle of the Screening Application

Screening at Very Low GM Levels Screening grain at very low GM levels can be accomplished by using a sufficiently large

sample size that tests negative for the GM trait. Lateral flow strips can be used by

testing multiple sub-samples, the size of which do not exceed the sensitivity of the strip

test. The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait sensitivity is at

least one RUR NK603 corn kernel in 800, one RUR canola seed in 1000, and one RUR alfalfa seed in 600.

The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait provides a yes/no answer for the

presence or absence of RUR seed in a given sample. Testing multiple statistically

selected sub-samples allows an estimate of the percent of RUR seed. The test results

provide information about the probability of the percent RUR seed in the sample.

Note: The test protocol does not determine the exact percent of RUR seed. It

determines the probability that a sample contains greater or less than a

specified threshold concentration.

Statistical Interpretation

The following tables provide information at five confidence levels with the use of

multiple samples of corn (400 kernels, 600 kernels, or 800 kernels each), canola

(1000 seeds each) and alfalfa (600 seeds each). The tables provide the maximum

percent GM levels that would be expected in the sample if all test-samples provide

negative results. The appropriate table can be used depending on the desired

screening level and how the samples will be processed.



Romer Labs Methods

Table C: 400 Kernel Sub-Samples (Corn) (All Sub-Samples Must be Negative)

No. of Sub-

Samples of

400 Kernels

Each

Maximum Percent RUR Kernels, Using Sub-Sample Sizes of 400 Kernels

at Five Different Confidence Levels (%)

50 75 90 95 99

1 0.17 0.35 0.58 0.75 1.2

2 0.087 0.18 0.29 0.38 0.58

3 0.058 0.12 0.20 0.25 0.39

4 0.045 0.085 0.15 0.19 0.29

5 0.035 0.070 0.12 0.15 0.25

6 0.029 0.058 0.10 0.13 0.20

7 0.025 0.050 0.085 0.11 0.18

8 0.022 0.044 0.075 0.10 0.15

Table D: 600 Kernel Sub-Samples (Corn) (All Sub-Samples Must be Negative)

No. of Sub-

Samples of

600 Kernels

Each

Maximum Percent RUR Kernels, Using Sub-Sample Sizes of 600 Kernels

at Five Different Confidence Levels (%)

50 75 90 95 99

1 0.12 0.23 0.039 0.50 0.78

2 0.058 0.12 0.20 0.25 0.39

3 0.039 0.077 0.13 0.17 0.27

4 0.029 0.058 0.10 0.13 0.20

5 0.024 0.047 0.077 0.10 0.16

6 0.019 0.039 0.065 0.085 0.13

7 0.017 0.033 0.055 0.075 0.11

8 0.015 0.029 0.050 0.065 0.096

Table E: 800 Kernel Sub-Samples (All Sub-Samples Must be Negative)

No. of Sub-Samples of

800 Kernels

Each

Maximum Percent RUR Kernels, Using Sub-Sample Sizes of 800 Kernels at Five Different Confidence Levels (%)

50 75 90 95 99

1 0.087 0.175 0.288 0.375 0.575

2 0.044 0.087 0.144 0.187 0.285

3 0.029 0.058 0.096 0.125 0.192

4 0.022 0.044 0.072 0.094 0.145

5 0.017 0.035 0.058 0.075 0.115

6 0.015 0.029 0.048 0.063 0.097

7 0.013 0.025 0.041 0.054 0.083

8 0.011 0.022 0.036 0.047 0.072



Romer Labs Methods

Table F: 1000 Seed Sub-Samples (Canola) (With Some Sub-Samples Positive)

No. of Sub-

Samples of

1000 Seeds

Each

No. of

Positive

Subsamples

Maximum Percent RUR Kernels, Using

Sub-Sample Sizes of 1000 Seeds at

Three Different Confidence Levels (%)

90 95 99

1 0 0.23 0.30 0.46

2 0 0.12 0.15 0.23

3 0 0.08 0.10 0.16

2 1 0.30 0.37 0.53

3 1 0.16 0.20 0.28

3 2 0.34 0.41 0.57

Table G: 600 Seed Sub-Samples (Alfalfa) (With Some Sub-Samples Positive)

No. of Sub-

Samples of

600 Seeds

Each

No. of

Positive

Subsamples

Maximum Percent RUR Kernels, Using

Sub-Sample Sizes of 600 Seeds at Five

Different Confidence Levels (%)

90 95 99

1 0 0.38 0.50 0.77

2 0 0.19 0.25 0.38

3 0 0.13 0.17 0.26

2 1 0.49 0.61 0.88

3 1 0.27 0.33 0.47

3 2 0.56 0.68 0.95

*All values determined using SeedCalc5



Romer Labs Methods

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AgraStrip® RUR-HS Bulk Grain

Strip Test – Semi-Quantitative

Method Part Number 7000011 Intended Use

The intended use of the kit is the semi-quantitative etermination of the CP4 EPSPS

protein in Roundup Ready® bulk corn and canola grain samples. The AgraStrip®

RUR-HS Bulk Grain Strip Test - Trait has a detection limit of one (1) Roundup

Ready® (CP4 EPSPS) corn kernel or canola seed in 1000 non-RUR corn kernels or

canola seeds in 5 minutes. Note: This application is intended for the

detection of NK603 Roundup Ready® corn (RUR NK603) and Roundup

Ready® canola. It does not detect genetic event GA21 Roundup Ready®

corn.

Product Description

The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait Test Kit detects the CP4

EPSPS protein produced by a gene, derived from Agrobacterium sp. strain CP4. This

gene has been incorporated into herbicide-resistant crops including Roundup Ready® and Roundup Ready® 2 brands (RUR) from Monsanto and other companies.

The lateral flow strips and other kit components are sufficient to detect the

presence or absence of the CP4 EPSPS protein in both field and laboratory

environments. This product application can semi-quantitatively screen for CP4

EPSPS grain.

Principle of the Assay

The assay uses a double antibody sandwich format. Antibodies specific to the CP4

EPSPS protein are coupled to a color reagent and incorporated into the lateral flow

strip. When the lateral flow strip is placed in a small amount of an extract from

plant tissue that contains CP4 EPSPS protein, binding occurs between the coupled

antibody and the protein. A sandwich is formed with some, but not all the antibody

that is coupled to the color reagent. The membrane contains two capture zones, one captures the bound CP4 EPSPS protein and the other captures color reagent.

These capture zones display a reddish color when the sandwich and/or unreacted

colored reagents are captured in the specific zones on the membrane. The

presence of only one line (control line) on the membrane indicates a negative

sample and the presence of two lines indicates a positive sample. The intensity of

the second line can be used to semi-quantitatively measure the level of CP4 EPSPS

grain present in a sample.

Contents of Kit Quantity

AgraStrip® RUR-HS Bulk Grain Strip Test- Trait 100

Conical microtubes, 1.5mL (COOLS2005) 100

Transfer pipettes (COOLS2010) 100



Romer Labs Methods

Materials Required but not Supplied:

Semi-Quantitative Extraction Buffer (7000103, makes 1L; 700104, makes 4L; or

7000105, makes 20L)

1L Nalgene bottle, for use with 7000103 (COSST5080)

4L Cubitainer, for use with 7000104 (COSST5081)

20L Cubitainer, for use with 7000105 (COSST5096)

Conical microtube rack (EQOLE1215)

Timer (EQOLE1301)

Graduated Cylinder, 50mL (EQOLE1051)

Balance, 400g (EQOLE1010)

Blender (110V: EQOLE1020; 220V: EQOLE1027)

Blender blade with gasket set (EQOLE1026)

AgraVision Reader (EQASR1007) and GMO Tray (EQASR1023)

Storage and Preparation of Reagents

The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait can be stored at room

temperature or refrigerated (2 -25ºC). Do not freeze. Once opened, the AgraStrip®

RUR-HS Bulk Grain Strip Test - Trait lateral flow test strips must be stored in the

closed canister with the moisture-indicating card. The moisture-indicating card must be

blue in color. If the moisture-indicating card is pink, contact Romer® Labs, Inc.

Technical Service. Storage conditions higher than room temperature may adversely

affect performance.

For Semi-Quantitative Bulk Grain Corn and Canola Testing:

If the semi-quantitative bulk corn grain method is to be run, the Semi-Quantitative

extraction buffer should be prepared according to the instructions on the bottle. For 7000103, the contents of the bottle are added to 1L of water in a 1L Nalgene bottle.

For 7000104, the contents of the bottle are added to 4L of water in a 4L Cubitainer.

For 7000105, the contents of the bottle are added to 20L of water in a 20L Cubitainer.

Purpose

The AgraStrip® RUR-HS Bulk Grain Strip Test - Trait Test Kit has been designed to

screen bulk grain samples for the presence of the CP4 EPSPS protein. Sample

preparation procedures are outlined below. Please refer to the instructions to be sure that all of the necessary materials are available prior to testing.

Sampling

The samples used for the AgraStrip® RUR-HS Bulk Grain Strip Test - Trait Corn Grain

Test Kit can be sub-samples of “representative samples” collected following GIPSA

sampling procedures from trucks, railcars, barges, etc. for other tests. The size of the

sub-samples to be used for the AgraStrip® RUR-HS Bulk Grain Strip Test - Trait will

depend on the percent RUR screening level desired and an acceptable level of risk that

the RUR level is close to the screening level.

Note: It is assumed that the samples collected are representative of the

contents of the truck or container and are sufficiently mixed to contain a

random distribution of the sample contents.



Romer Labs Methods

Sample Preparation

The sample is ground and then extracted with buffer in a polypropylene “Mason”-type jar. The sample preparation is important for the proper function of the test, especially

the ratio of buffer to the weight of the seed sample. The size of “Mason” jar required

and the grinding time depends on the sample size to be analyzed. Table B lists those

parameters.

Corn: Sample Weight (g) x 2.0 = Buffer Volume (mL)

Canola: Sample Weight (g) x 3.0 = Buffer Volume (mL)

Table B: Parameters for Preparing Samples

Number of Kernels in

Sample Jar Size (oz.) Grind Time (sec)

Corn: 25 – 125 4 10 – 20

Corn: 125 – 250 8 15 – 25

Corn: 250 – 500 16 20 – 35

Corn: > 500 32 45 – 60

Canola: 1000 4 10 – 15

The processing parameters were determined using the laboratory grade Waring Model

31BL91 food processor with the standard blade. Other food processors may require

different parameters. All kernels should be ground.

Sample Processing

1. Weigh sub-samples from each truck or container.

2. Place each sub-sample in a clean, dry polypropylene “Mason” jar of the

appropriate size. See Table B.

3. Attach the jar adapter and clean, dry cutting blades.

4. Place the jar onto the food processor, place a shield over the jar if desired, and

grind the sub-sample on high speed for the time indicated in Table B.

Caution: If using glass “Mason” jars, it is recommended to shield the jars

during grinding with a “tri-cornered” 1-liter plastic beaker. 5. Remove the adapter and cutting blades.

Note: A coffee grinder may also be used to grind small sample sizes (i.e.

canola). If a coffee grinder is used, transfer the sample to a clean container

for extraction.

6. Add the volume of buffer (see below) to the ground seed in the jar, place a lid on

the jar and shake the jar until all the ground corn is well wetted (about 10-20

sec.)

For Corn: Sample Weight (g) X 2.0 = Buffer Volume (mL)

For Canola: Sample Weight (g) X 3.0 = Buffer Volume (mL)

Note: The sample will have a “thick” consistency but should contain some free liquid

after a short settling time. There should be no whole seeds remaining.

7. Use this free liquid as sample in the Test Procedure.



Romer Labs Methods

Test Procedure – Semi-Quantitative Bulk Grain Corn and Canola

1. Transfer some liquid from the prepared sample to a conical microtube by

squeezing the bulb of the transfer pipette and inserting it in the free liquid in the

top of the sample jar.

2. Release the bulb to pull up the sample. Add the sample from the pipette to the

sample tube by squeezing the bulb. This should be approximately 0.5 mL.

3. Place one AgraStrip® RUR-HS Bulk Grain Strip Test - Trait Test Strip into the

sample tube. Let sit for 5 minutes.

4. The appearance of one line (control) on the strip indicates a negative result. 5. The appearance of two lines on the strip indicates a positive result.

6. Cut off the bottom, colored portion of the strip using scissors.

Discard this portion.

7. Place the top white-colored strip into the AgraVision Reader for

semi-quantitative analysis. The cut end of the strip points towards the

number printed on the tray.

Interpreting the Lateral Flow Strip Test

Check the test strips 5 minutes after inserting the strip. At least one line, the Control

Line, should always develop approximately one (1) cm up from the bottom sample pad. A red line in this position indicates that the strip is functioning properly. The strip

must be left in the extract for the entire 5 minute incubation period if it is to be read

for semi-quantitative analysis. For semi-quantitative analysis, insert the strip into the

AgraVision Reader and read. The AgraVision Reader will display the result as a range

of %GMO (i.e. <0.5%, 0.1 – 0.5%, 0.5 – 1.0%, 1.0 – 5.0%, or >5%). If no control

line develops, the result is inconclusive and needs to be repeated.

Note: Test strip results should be interpreted after 5 minutes. Test strips

interpreted after 30 minutes are invalid.

The sample tube has a 0.5-mL

indicator at the top of the tapered

section.



Romer Labs Methods

Equipment Cleaning and Drying

Caution: It is important to clean and dry the jars and cutting blades and/or coffee

grinder between samples.

1. The “Mason” jar should be emptied, rinsed thoroughly with water and completely

dried with a paper towel between uses.

2. The cutting blades for the blender should be rinsed with water until all ground

seed is removed, washed using standard household liquid soap, rinsed well and carefully dried. If available, spraying or rinsing with methanol or isopropyl

(rubbing) alcohol will assist drying.

3. Coffee grinders should be emptied, wiped down with moist towels thoroughly and

completely dried with paper towels between uses. Special attention should be

paid to the area at the base of the blades. It is not advisable to put water directly

into the grinder. Be sure to unplug the grinder when cleaning.

Exclusion of Warranty Statement The user assumes all risk in using Romer Labs®, Inc. products and services. Romer®

Labs, Inc. will warrant that its products and services meet all quality control standards

set by Romer Labs®, Inc., and Romer Labs®, Inc. will, at its option, repair or replace

any product, components, or repeat services which prove to be defective in

workmanship or material within product specific warranty periods or expiration dates

and which our examination shall disclose to our satisfaction to be defective as such.

This warranty is expressly in lieu of all other warranties, expressed or implied, as to

description, quality, merchantability, fitness for any particular purpose, productiveness,

or any other matter. Romer Labs®, Inc. shall be in no way responsible for the proper use of its products. Romer Labs®, Inc. hereby disclaims all other remedies, warranties,

guarantees or liabilities, expressed or implied, arising by law or otherwise, and it shall

have no liability for any lost profits or damage, direct, indirect or otherwise, to person

or property, in connection with the use of any of its products or services. This warranty

shall not be extended, altered or varied except by a written instrument signed by an

authorized representative of Romer Labs®, Inc.

For Technical Service Call:

Romer Labs®, Inc.

1301 Stylemaster Dr. Union, MO 63084

Phone: (636) 583-8600

Fax: (636) 583-2340

AgraStrip RUR-HS Bulk Grain Y Qualitative Method Strip Test · kernels in 5 minutes, one (1)...

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Transcript of AgraStrip RUR-HS Bulk Grain Y Qualitative Method Strip Test · kernels in 5 minutes, one (1)...