Additional figure 1 Ballini et al

0N 1N 0N 1N 0N 1NSL4- CD203 SL4- CL32 SL4- Guy11

0N 1N 0N 1N 0N 1NSL4- TH16 SL4- US31 SL4-VT2

0N 1N 0N 1N 0N 1NSL5- CD203 SL5- CL32 SL5- Guy11

0N 1N 0N 1N 0N 1NSL5- TH16 SL5- US31 SL5-VT2

0N 1N 0N 1N 0N 1NSL19- CD203 SL19- CL32 SL19- Guy11

0N 1N 0N 1N 0N 1NSL19- TH16 SL19- US31 SL19-VT2

0N 1N 0N 1N 0N 1NKasalath-CD203 Kasalath-CL32 Kasalath-Guy11

0N 1N 0N 1N 0N 1NKasalath-TH16 Kasalath-US31 Kasalath-VT2

0N 1N 0N 1N 0N 1NNipponbare-CD203 Nipponbare-CL32 Nipponbare-Guy11

0N 1N 0N 1N 0N 1NNipponbare-TH16 Nipponbare-US31 Nipponbare-VT2

CD20

3CL

32GU

Y11

TH16

US31 VT

2CD

203

CL32

GUY1

1TH

16US

31 VT2

CD20

3CL

32GU

Y11

TH16

US31 VT

2CD

203

CL32

GUY1

1TH

16US

31 VT2

CD20

3CL

32GU

Y11

TH16

US31 VT

2

Nipponbare Kasalath CSSL4 CSSL5 CSSL19

0.0

0.2

0.4

0.6

0.8

1.0

1.2

1.4

0N 1N

Num

ber o

f sus

cepti

ble

lesio

n/le

af su

rfac

e ar

eaAdditional figure 2 Ballini et al

****

** *

**

** **

***

***

******

******

***

***

******

***

*** **

*

Additional Table 1 NIS across rice and M. oryzae diversity

Guy1

1CD

203

CL26

Nip

ponb

are

tem

pera

te ja

poni

caRo

bust

par

tial R

esis

tanc

eRo

bust

par

tial R

esis

tanc

eRo

bust

par

tial R

esis

tanc

eIR

64in

dica

Robu

st co

mpl

ete

Resi

stan

ceRo

bust

com

plet

e Re

sist

ance

Robu

st co

mpl

ete

Resi

stan

ceTr

embe

setr

opic

al ja

poni

caM

ore

susc

eptib

le w

ith N

itrog

enM

ore

susc

eptib

le w

ith N

itrog

enRo

bust

par

tial R

esis

tanc

eIR

8in

dica

Robu

st co

mpl

ete

Resi

stan

ceRo

bust

com

plet

e Re

sist

ance

Mor

e su

scep

tible

with

Nitr

ogen

Mor

ober

eken

trop

ical

japo

nica

Robu

st p

artia

l Res

ista

nce

Robu

st p

artia

l Res

ista

nce

Robu

st p

artia

l Res

ista

nce

Pao

Tou

Hung

indi

caM

ore

susc

eptib

le w

ith N

itrog

enM

ore

susc

eptib

le w

ith N

itrog

enRo

bust

par

tial R

esis

tanc

ePe

tita

jang

gut

indi

caM

ore

susc

eptib

le w

ith N

itrog

enna

Mor

e su

scep

tible

with

Nitr

ogen

Ta M

ao T

aote

mpe

rate

japo

nica

Mor

e su

scep

tible

with

Nitr

ogen

naM

ore

susc

eptib

le w

ith N

itrog

enAz

ucen

atr

opic

al ja

poni

caM

ore

susc

eptib

le w

ith N

itrog

enna

Robu

st p

artia

l Res

ista

nce

Mar

atel

lite

mpe

rate

japo

nica

Mor

e su

scep

tible

with

Nitr

ogen

naM

ore

susc

eptib

le w

ith N

itrog

enKa

sala

thAu

sM

ore

susc

eptib

le w

ith N

itrog

enM

ore

susc

eptib

le w

ith N

itrog

enM

ore

susc

eptib

le w

ith N

itrog

enCh

uan

4te

mpe

rate

japo

nica

Mor

e su

scep

tible

with

Nitr

ogen

Mor

e su

scep

tible

with

Nitr

ogen

Mor

e su

scep

tible

with

Nitr

ogen

Popo

t 165

indi

caM

ore

susc

eptib

le w

ith N

itrog

enM

ore

susc

eptib

le w

ith N

itrog

enM

ore

susc

eptib

le w

ith N

itrog

enPa

di B

oeno

rin

dica

Mor

e su

scep

tible

with

Nitr

ogen

Mor

e su

scep

tible

with

Nitr

ogen

Mor

e su

scep

tible

with

Nitr

ogen

Mag

napo

rthe

ory

zae

isol

ate

rice accession

Additional Table 2 Recombinant lines establishing NIS1 on chromosome 1

11

24

44

49

RM11

219

RM59

31

RM40

6 RM

551

RM16

502

RM24

1 RM

3335

RM

2386

5 Ph

enot

ype

unde

r high

N4-

40-7

ka

s ka

s N

ip

Nip

N

ip

Nip

N

ip

Nip

M

ore

Susc

eptib

le4-

40-8

ka

s ka

s N

ip

Nip

N

ip

Nip

N

ip

Nip

M

ore

Susc

eptib

le4-

40-2

0 ka

s ka

s ka

s ka

s ka

s N

ip

Nip

N

ip

Mor

e Su

scep

tible

19-2

4 ka

s ka

s N

ip

Nip

N

ip

Nip

N

ip

Nip

M

ore

Susc

eptib

le

lines

chro

mos

ome

and

micr

osat

telite

mar

kers

Additional Table 3 Further mapping of the NIS1 locus

More susceptible in 1N No difference in 1N and 0N

Marqueur Position (Mbp) CSSL4 CSSL5 CSSL19 19-42-4 19-42-2 CSSL9

RM7075 15.12 Nip Nip Kas Kas Kas KasRM11007 17.95 Nip Nip Kas Kas Kas KasRM1196 21.90 Kas Nip Kas nd nd ndRM11219 22.16 Kas Nip Kas Nip Nip ndRM5718 22.93 Kas Nip Kas Nip Nip NipRM6716 23.12 Kas Nip Nip Nip Nip NipRM2318 24.14 Kas Nip Nip Nip Nip NipRM3440 27.19 Kas Kas Nip Nip Nip NipRM7419 27.70 Kas Nip Nip Nip Nip ndRM7566 27.77 Kas nd Kas Kas Nip ndRM11522 28.07 Kas Kas Kas Kas Nip NipRM11504 28.11 Kas Kas Kas Kas Nip NipRM11563 29.16 Kas Kas Kas Kas Nip NipRM5931 29.28 Kas Kas Kas Kas Nip NipRM11581 29.46 Kas Kas Kas Kas Nip NipRM11594 29.62 Kas Kas Kas Kas Nip NipRM6702 29.77 Kas Kas Kas Kas Nip NipRM3615 29.81 Kas Kas Kas Kas Nip NipRM1152 30.09 Kas Kas Kas Nip Nip ndRM11631 30.52 Kas Kas Kas Kas Nip ndRM11646 30.71 Kas Kas Kas Kas Nip ndRM1095 30.92 Kas Kas Kas Nip Nip ndRM11658 31.04 Nip Kas Kas Nip Nip ndRM8097 31.41 Nip Kas Kas nd nd ndRM11668 31.42 Nip Kas Kas Nip Nip nd

Concentration (mM) elements 0N 1N

Macronutrient

K2SO4 1.34 1.34KH2PO4 1.97 1.97NH4NO3 0.00 0.50MgSO4, 7H2O 1.53 1.53

Micronutrient

H3BO3 0.91 0.91MnSO4 0.50 0.50(NH4)6Mo7O24,4H2O 0.01 0.01CuSO4, 5H2O 0.08 0.08ZnSO4, 7H2O 0.31 0.31

Ferveg (6% Fe) H2O pH 5,5

Additional Table 4 Composition of fertilizing solution

Additional Table 5 Primer sequences for q-PCR experiments

Gene name Annotation Accession Sequence Forward Primer Sequence Reverse PrimerPR5 protein PR (pathogenesis related) Os12g43430 CGCTGCCCCGACGCTTAC ACGACTTGGTAGTTGCTGTTGCPBZ1 protein PR (pathogenesis related) Os12g36880 CCTGCCGAATACGCCTAAGATG AGAACACATTCAGACTTGCCTCTCOsMT2b Os05g02070 ACCGTCGTCGTCGTTGTCAG GCATGAGGAGATGGAGCAGGAGPAL Os02g41650 TTCTTGATCGCCCTGTGCCAAG TGCATCACGCAGTTCTTCACCGlucanase protein PR (pathogenesis related) Os01g71340 CGTTCCATATATAGTTTGGGTTTG TCAAGTTCATATTATTTGTCTCTGSubtilase protein PR (pathogenesis related) Os01g58290 CGGAAAGTTGCTGACCCATTCG GCTCGCCGCACCTTGATTGPR3 protein PR (pathogenesis related) Os04g41620 CGTGTCTGTGGAGAGCGTGGTC TCGTCGTTGGTGCGGTCATTGGPOX223 protein PR (pathogenesis related) Os07g48020 GCTCATCCCCATCAACAACT GTGCACTGGATTGACCAG