2018 Grape Day Grape Day Business and Economics from Vineyard to Wine Sales LaSells Stewart Center,...
Transcript of 2018 Grape Day Grape Day Business and Economics from Vineyard to Wine Sales LaSells Stewart Center,...
2018GrapeDayBusinessandEconomicsfromVineyardtoWineSales
LaSellsStewartCenter,OSUCampus,April3
AbstractsofPresentationsandPosters
ProgramSchedule
2018OWRIGrapeDay Page1
8:30AM– 9:00AM RegistrationandRefreshments9:00AM– 9:05AM IntroductionandWelcome;
MarkChien,ProgramCoordinator
9:05AM– 9:50AM AStepTowardsMoreSustainableVineHealth:TheCleanPlantApproachtoGrapeVirusDiseaseManagementDr.NeilMcRoberts,AssociateProfessorofPlantPathology,UCDavis
10:00AM–10:45AM UsingEconomicAnalysistoInformBusinessDecisions‐ExamplesfortheOregonWineIndustryDr.CatherineDurham,AssociateProfessor,DepartmentofAppliedEconomics,OSU
10:45AM–11:45AM InteractivePosterSessionThissessionwillprovideyoutheopportunitytointeractone‐on‐onewithscientistsandstudentsconductingresearchattheOWRI.Posterswillfeatureresearchfindingsthatarein‐progressforvariousresearchprojectsbeingconductedacrossawidearrayoftopicswithinviticulture,enology,andeconomics.
11:45AM–12:30PM ValuingOregon’sWineGrowingRegionsDr.RobinCross,AssistantProfessorandSeniorResearcher,DepartmentofAppliedEconomics,OSU
12:30PM– 1:30PM Lunch
1:30PM– 2:15PM AreVineyardBuyersWarmingUptoCoolerLocations?QuantifyingtheValueofLocationSpecificAdaptabilityJasonBeasley,GraduateStudent,DepartmentofAppliedEconomics,OSU
2:15PM– 2:45PM Break/PosterSession
2:45PM– 3:30PM BenefitsofEarlyAdoptionofPreventativePruningPracticesinManagingGrapevineTrunkDiseasesDr.JonathanD.Kaplan,Professor,Dept.ofEconomics,
3:30PM– 3:45PM FinalQ&Aandprogramwrap‐up
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# PosterTitle Author(s) Page
ViticultureandVinePhysiology
1 Re‐EvaluatingFieldMethodsofWaterStatusDeterminationintheVineyard
AlexanderLevin,TannarWilliams,RebeccaLake 7
2 Post‐VeraisonWaterDeficitImprovesPinotNoirFruitQualityWithoutaYieldPenalty
AlexanderLevin,JudyChiginsky,ChristopherJenkins
8
3 DevelopingaModelSystemtoIdentifyMainMechanismsInvolvedinNitrogenGrowthResponsesofGraftedGrapevines
LandryRossdeutsch,R.PaulSchreiner,PatriciaA.Skinkis,JosephOrton,LaurentDeluc
9
4
PerformanceofTaxonomicallyDiverseArbuscularMycorrhizalFungiIsolatedfromaRed‐HillSoilinSymbiosiswithPinotNoir
R.PaulSchreiner,DavidP.Janos,TianTian
10
5
ComparingNitrogenAdditionintheVineyardVersusintheWineryonPinotNoirandChardonnay
TianTian,R.PaulSchreiner,PatriciaA.Skinkis,JamesOsborne,ElizabethTomasino
11
6
UnderstandingtheImpactofDormantPruningMethodonPinotNoirBudFruitfulnessandYield
MirandaR.Ulmer,PatriciaA.Skinkis 12
GrapeGenomicsandGenetics
7RoleofAuxin‐ResponseFactor4intheTimingofRipeningInitiationinVitisvinifera
SatyanarayanaGouthu,VictorM.Puoci,GraceCheng,LaurentG.Deluc
13
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# PosterTitle Author(s) Page
EnologyandSensoryChemistry
8TheEffectofYeastAssimilableNitrogenonSO2ProductionbySaccharomycesCerevisiaeDuringWineFermentation
MaríaAlessandri,AlanBakalinsky 14
9ImpactofPre‐FermentationColdSoakConditionsonPinotNoirWineAromaCompounds
MengyingFu,JaredJohnson,JamesOsborne,MichaelQian
15
10TheUseofSignalDetectionTheory(d’)inFoodSensoryAnalysis RachelHahn,Elizabeth
Tomasino,ClaudioFuentes
16
11InvestigatingFruitinessAromaPerceptioninWhiteWines AngelicadeCastroIobbi,
ChaseJutzi,ElizabethTomasino
17
12 EffectsofCropLoadonPinotNoirWineComposition JingwenLi,PatriciaA.Skinkis,MichaelQian 18
13Useofnon‐SaccharomycesYeasttoReduceVolatileAcidityProductionDuringColdSoakingofPinotNoirGrapes
JaredJohnson,MengyingFu,MichaelQian,JamesOsborne
19
14 InfluenceofYeastProductionAdditionandFermentationTemperatureonChangesinLipidCompositionsofPinotNoirWines
QuynhPhan,JamesOsborne,ElizabethTomasino
20
15 ComparativeAnalysesoftheTotalCarbohydrateCompositionofRedWinePolysaccharides
LudwigRing,ElizabethTomasino,JamesOsborne,MichaelQian 21
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# PosterTitle Author(s) Page
PestandDiseaseManagement
16 FieldObservationsofTortistilusSpeciesAssociatedwithOregonVineyards
DanielT.Dalton,RichardJ.Hilton,VaughnM.Walton 22
17DeficitIrrigationandGrapevineRedBlotchDiseaseManagement
AchalaKC,AnnRasmussen,AlexanderLevin
23
18InteractionofDeficitIrrigationandGrapevineRedBlotchVirus(GRBV)onDiseaseDevelopmentandGrapevinePhysiology
AlexanderLevin,ChristopherJenkins,JudyChiginsky,AnnRasmussen,AchalaKC
24
19ExogenousApplicationofAbscisicAcid(s‐ABA)DoesNotImproveFruitCompositioninRedBlotch‐InfectedVines
AlexanderLevin,ChristopherJenkins,JudyChiginsky,AnnRasmussen,AchalaKC,DanielDalton,VaughnWalton
25
20 ExploringtheSymptomologyandImpactofRedBlotchDiseaseonPinotnoir
JustinLitwin,PatriciaSkinkis,RobertMartin 26
21RapidSamplingTechniquestoDetermineQoIFugicideResistanceinErysipheNecator
SarahR.Lowder,TaraNeill,MichelleM.Moyer,TimothyD.Miles,WalterMahaffee
27
22 CleanGrapevines‐TowardaHarmonizedGrapevineCertificationProgramforthePacificNorthwest
RobertMartin 28
23 ARegionalMildewManagementProblem‐FungicideResistanceintheWest
TaraNeill,SarahR.Lowder,TimothyD.Miles,WalterMahaffee,MichelleM.Moyer,MonicaCooper
29
24EarlyBloomTimingforBetterGrapevinePowderyMildewManagementinWesternOregon
JayW.Pscheidt,JohnBassinette 30
25 AStepTowardsMoreSustainableVineHealth:theCleanPlantApproachtoGrapeVirusDiseaseManagement
NeilMcRoberts,KamyarAram,KariArnold,DeborahGolino
31
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# PosterTitle Author(s) Page
Economics
26 AreVineyardBuyersWarmingUptoCoolerLocations?QuantifyingtheValueofLocationSpecificAdaptability
W.JasonBeasley,RobinM.Cross 32
27 ValuingOregon’sWineGrowingRegions RobinM.Cross 33
28 UsingEconomicAnalysistoInformBusinessDecisions–ExamplesfortheOregonWineIndustry
JamesSterns,CatherineDurham
34
29
BenefitsofEarlyAdoptionofPreventativePruningPracticesinManagingGrapevineTrunkDiseases
JonathanKaplan,RenaudTravadon,MonicaCooper,VickenHillis,MarkLubell,KendraBaumgartner
35
30
ProtectingyourcashflowusingWholeFarmRevenueProtectionandOtherFarmFinancialManagementTools
KentFleming,BeauOlen 36
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Re‐evaluatingFieldMethodsofWaterStatusDeterminationintheVineyard
AlexanderLevin1*,RebeccaLake2,TannarWilliams3
1AssistantProfessor,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;2StudentTechnicalAssistant,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;3StudentTechnicalAssistant,DepartmentofChemicalEngineering,StanfordUniversity,StanfordCA
*CorrespondingAuthor:[email protected],541‐772‐5165
Thepressurechamberisawidelyusedtoolforassessingwaterpotential(Ψ)inplantsandiscommonlyusedasatoolinvineyardirrigationscheduling.Althoughitisregardedasthemostrobustmethodforassessmentofplantwaterstatusinthefield,therecontinuestobedisagreementamongusersaboutpropertechniqueforbothmiddayleaf(Ψleaf)andmiddaystem(Ψstem)waterpotentialdetermination.Toresolvethesediscrepancies,threeexperimentswereperformedinordertounderstandhowvariedtechniquesaffecteitherΨleafandΨstemvalues:(1)Ψleafresponsetotimeintervalbetweensampleexcisionandpressurization(2)Ψleafresponsetosamplepreparationmethod(e.g.petiolere‐cutting)priortopressurization;and(3)Ψstemresponsetosampleequilibrationtime.Allexperimentswereperformedbytwooperatorsusingthesameinstrument.TherewerenosignificanteffectsoftimeintervaloroperatoronΨleafattimeintervalsfrom15to60s.FewsignificantdifferenceswerefoundinΨleafamongsamplepreparationmethods(experiment2),andtheydependedonoperator.Ψstemvaried5%whensampleswereallowedtoequilibratefrom10to240minutes(min)priortodetermination.Theresultsshowthattimeintervalsofupto60sbetweenexcisionandpressurizationareacceptableforaccuratedata,andpetiolere‐cuttingdoesnotsubstantivelyaffectΨleafdetermination.Additionally,Ψstemequilibrationtimescanbeasshortas10min.However,significantdifferenceswereobservedbetweenoperatorsacrossallthreeexperiments.Thus,thetechnicalskilloftheoperatorduringpressurizationmayplayalargerroleintheoutcomeofthedeterminationrelativetothepreparationofthesamplepriortopressurization.
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Post‐VeraisonWaterDeficitsImprovePinotNoirFruitQualityWithoutaYieldPenalty
AlexanderLevin1*,ChristopherJenkins2,JudyChiginsky3
1AssistantProfessor,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;2FacultyResearchAssistant,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;3BiologicalSciencesResearchTechnicianII,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR
*CorrespondingAuthor:[email protected],541‐772‐5165
DespiteincreasingPinotnoiracreageinwarmerandmorearidgrowingregions,cultivar‐specificdroughtresponsesremainpoorlyelucidatedintheliterature.Amulti‐yearfieldexperimentwasestablishedwitheightirrigationtreatmentsdesignedtoaltervinewaterstatuseitherpre‐orpost‐veraison.Irrigationwasscheduledbasedonapplyingwateratfractionsofestimatedcropevapotranspiration(ETc)rangingfrom25to100%.Vinewaterstatuswasmonitoredwithregularmeasurementsofmiddaystemwaterpotential(Ψstem)throughoutthegrowingseason.Atharvest,fruitwereanalyzedforyieldandqualitycharacteristics.Thetreatmentssignificantlyalteredvinewaterstatusbothpre‐andpost‐veraison,givingrisetofourlevelsofwaterstressatbothtimes.Berrysizewasnegativelycorrelatedwithwaterdeficitsatbothtimes.Berryprimarymetabolism(Brix,pH,andTA)waslessresponsivetowaterdeficitsrelativetosecondarymetabolism(polyphenolics).Totalanthocyaninsincreasedwithwaterdeficitsbothpre‐andpost‐veraison,andtheresponsewasmoresensitivepre‐veraison,butthedifferenceswerenotsignificantacrosstreatments.Incontrast,tanninsandiron‐reactivephenolics(IRPs)inskinsandseedsweresignificantlyimpactedbythetreatments.SkintanninsandIRPsincreasedwithpre‐veraisonwaterdeficits,butdecreasedwithpost‐veraisonwaterdeficits.SeedtanninsandIRPsincreasedwithpre‐veraisonwaterdeficits,butwerenotaffectedbypost‐veraisonwaterdeficits.Ingeneral,berrysecondarymetabolismwasmoresensitivetopre‐veraisonwaterdeficits.However,post‐veraisonwaterdeficitsresultedinhigherconcentrationsofsecondarymetabolitesoverall.Whilewinesensoryanalyseshaveyettobecompleted,theresultssuggestthatpost‐veraisonwaterdeficitsmaybemoreeffectiveatimprovingPinotnoirfruitqualitywithoutayieldpenaltycomparedtopre‐veraisondeficits.
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DevelopingaModelSystemtoIdentifyMainMechanismsInvolvedinNitrogenGrowthResponsesofGraftedGrapevines
LandryRossdeutsch1*,R.PaulSchreiner2,PatriciaA.Skinkis3,JosephOrton4,LaurentDeluc5
1Post‐DoctoralResearchAssociate,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;2ResearchPlantPhysiologist,USDA‐ARS‐HCRU,CorvallisOR;3AssociateProfessor&ViticultureExtensionSpecialist,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;4UndergraduateResearchAssistant,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;5AssociateProfessor,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐2982
Thesciongrowthpotential(vigor)ingraftedgrapevinesresultsfromthethree‐wayinteractionbetweenenvironment,sciongenotype,androotstockgenotype.Sincenitrogen(N)availabilityisamajordriverofgrapevinegrowth,understandingNregulationinscionandrootstockwillleadtonewinsightstocontrolcanopysizeinvineyards.WearedevelopingamodelsystemtostudyNregulationbyevaluatingtheNsupplyresponsesof12scion‐rootstockcombinationswithknowndifferencesinscionandrootstockvigor.Ourprimaryobjectivesaretounderstandtheinfluenceofscionsandrootstocksongrowthparametersandresourceallocation,andtoevaluatetheroleofNuptakeregulationinsciongrowthresponse.Toaddressthefirstobjective,wemeasuredcomponentsofvinewaterrelationsandgasexchange,plantbiomass,carbon(C),andNallocationinfourplantstissues(leaves,stem,trunkandroots).PreliminaryresultssupportedtheexpectedvigorbehaviorofthethreePinotnoirscionsusedinthisexperiment,butthiswasnottrueforthefourrootstocksexamined.NitrogenavailabilityalteredCandNallocationinalltissues,butscionvigorwasnotaffected.ItseemsthattheNrequirementfor1‐yearoldvineswassatisfiedbyourlowestNrate,andtheexperimentwillberepeatedundergreaterNlimitation.However,thisfirsttrialwillallowustostudytheroleofCandNreservesonscionvigorduringthesecondgrowingseason.WeareaddressingthesecondobjectivebycomparingNuptakeandNtransportamongtworootstocksusing15NO3.SeveralexperimentsareunderwaytocompareNuptakekineticsoverarangeofNconcentrationsandNtransportrateinresponsetoplantNstatus.TheseanalyseswillbecomplementedwithgeneexpressionstudiestargetingthetransportandsignalingofNinrootsandleaves.
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PerformanceofTaxonomicallyDiverseArbuscularMycorrhizalFungiIsolatedfromaRed‐HillSoilinSymbiosiswithPinotNoir
R.PaulSchreiner1*,DavidP.Janos2,TianTian3
1ResearchPlantPhysiologist,USDA‐ARS‐HCRU,CorvallisOR;2Professor(retired),UniversityofMiami,CoralGablesFL;3GraduateResearchAssistant,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐738‐4084
Grapevinesrelyonarbuscularmycorrhizalfungi(AMF)toobtainamplephosphorus(P)fromsoilswithmoderatetolowPlikethered‐hillsoilsinwesternOregon.PriorresearchusingDNAsequencingindicatedthat6‐11speciesofAMFcolonizedtherootsofPinotnoirwithinagivenvineyard.However,littleisknownabouthowdifferentspeciesofAMFfunctioninvineyardecosystems.WeinvestigatedtheeffectivenessoffivenativeAMFspeciesrepresentingfivegeneratopromotegrowthandnutrientuptakeofPinotnoirinared‐hillsoilunderwell‐wateredanddroughtedconditions.RootedcuttingsweregrowninthepresenceoffivedifferentAMForwithoutAMF,andwithorwithoutmoderatedroughtstress.After8and16weekswholevinesweredestructivelyharvestedandbiomassandnutrientuptakeweredetermined.ResultsshowedthatfourofthefiveAMFcolonizedrootswell,increasedrootandshootbiomass,andpredominantlyincreasedPuptake.However,theClaroideoglomusisolatewassuperiorinpromotingshootgrowthandPmovementtoshoots.TheabilitytoenhancevinegrowthandPuptakewasnotrelatedtotheextentofarbusculesinrootssuggestingthatsomePexchangemayoccurviahyphaeinsideroots.Potassiumandironuptakewereenhancedalsobythefoureffectiveisolates.WaterlimitationreducedPuptakeinthissoilasamaineffectacrossallAMFtreatments,indicatingthatwaterstressreducesthecapacityforPuptakebyalltheAMFspeciesstudiedhereinasimilarmanner.ThesefindingssuggestthatAMFwithinClaroideoglomuswouldbethebestchoiceforinoculatingyounggrapevinestomaximizeestablishmentinlowPsoils.
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ComparingNitrogenAdditionintheVineyardversusintheWineryonPinotNoirandChardonnay
TianTian1,R.PaulSchreiner2*,PatriciaA.Skinkis3,JamesOsborne4,ElizabethTomasino5
1GraduateResearchAssistant,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;2ResearchPlantPhysiologist,USDA‐ARS‐HCRU,CorvallisOR;3AssociateProfessor&ViticultureExtensionSpecialist,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;4AssociateProfessor&EnologyExtensionSpecialist,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR;5AssistantProfessor,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐738‐4084
Theimpactofnitrogen(N)additionsinthevineyardonvineproductivityandwinecharacteristicsascomparedtowineryNadditionsonthelatterisbeingevaluatedinPinotnoir(PN)andChardonnay(CH).Fivetreatments,includingnovineyardorwineryNaddition(Control),additionofdiammoniumphosphateinthewinery(+DAP),additionoforganic‐Ninthewinery(+OrgN),additionofNinthevineyardtothesoil(+SoilN)ortothefoliage(+FoliarN)wereestablishedwithfourreplicatesforeachvariety.The+FoliarNtreatmentbeganin2017,whiletheotherfourtreatmentswereevaluatedin2016and2017forCH.ForPN,allfivetreatmentsbeganin2017.Inbothvarieties,+SoilNapplicationincreasedleafNstatusandfruityeastassimilablenitrogen(YAN)levels,butdidnotalteryield,leafarea,orvinewaterstatusinthefirstyear.FoliarNapplicationalsoincreasedfruitYANinthefirstyearwithoutincreasingleafNorvinegrowth.VinegrowthandyieldincreasedinCHinresponseto+SoilNinthesecondyearalongwithleafandfruitNstatus.Rootcolonizationbyarbuscularmycorrhizalfungi(AMF)wasreducedinbothvarietiesinthe+SoilNvineswithgreatereffectinCHwhereNwasappliedfortwoyears.VinePstatusdecreasedalsoinCHinthesecondyearinconcertwithlowerAMF.WineryNadditionsboostedmustYANlevelstoroughlymatchthoseofthe+SoilNand+FoliarNmusts.MustfermentationinCHproceededmorerapidlywhereNwasaddedinthevineyardorthewinery,whilefermentationinPNwasincreasedonlybyvineyardNaddition.Sensoryevaluationof2016CHwinesshowedthat+SoilNwinesdifferedfromwinesofControl,+DAP,and+OrgN.
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UnderstandingtheImpactofDormantPruningMethodonPinotNoirBudFruitfulnessandYield
MirandaR.Ulmer1,PatriciaA.Skinkis2*
1GraduateResearchAssistant;2AssociateProfessor&ViticultureExtensionSpecialist,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐1411
Oregon‘Pinotnoir’producersprimarilyusecanepruning,astheybelievespurpruningresultsinseverelyreducedyieldsduetoalackoffruitfulbasalbuds.However,spurpruningprovidesanopportunitytopartiallymechanizepruningoperations,therebyincreasingvineyardlaborefficiency.Atwo‐yearstudywasinitiatedinacommercialvineyardtodeterminetheviabilityofspurpruning‘Pinotnoir’inOregon.Budfruitfulness,ameasureofthepotentialfruitfulnessofthevineandamajorcomponentofyield,wasmeasuredoncaneandspurprunedvinesduringJanuary2017and2018.Canopygrowth,yieldpredictorsandharvestyielddataweremeasuredthroughoutthegrowingseasononcaneandspurprunedvines.Theresultsshowspur‐prunedvineshavefruitfulbasalbuds.During2017,budfruitfulnesswassimilaratallnodepositionsincaneandspurprunedvinesexceptfornodethreewherespurprunedvineshadhigherbudfruitfulness.In2018,cane‐prunedvineshadgreaterbudfruitfulnessthanspur‐prunedvinesatnodefive.Intheearlyspringtherewere5moreinflorescencepervineoncane‐prunedvines.Inflorescencesofcane‐prunedvineshadmorefloretsandberriespost‐fruitsetthanspurprunedvines,butthepercentoffloretsturnedtoberries(fruitset)didnotdifferbetweenthetwopruningmethods.Atharvest,spur‐prunedvineshadmoreclusterspervinewhereascane‐prunedvineshad27%heavierclusters,buttherewasnodifferenceinwholevineyield.Basicfruitripenessatharvestdidnotdifferbetweenthetwopruningmethods.Arelationshipwasevidentbetweenvinevigorandbudfruitfulnessparameters.In2017,largerinflorescenceprimordiaoftheprimarybudwereobservedwithlargerinternodediameter.In2018,higherbudfruitfulnesswasobservedwithgreatercaneweights.Thisworkwillcontinueintothe2018growingseasonandwinterof2019.
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RoleofAuxin‐ResponseFactor4(VitviARF4)intheTimingofRipeningInitiationinVitisvinifera
SatyanarayanaGouthu1,VictorM.Puoci2,GraceCheng3,LaurentG.Deluc4*
1Post‐DoctoralResearchAssociate,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;2UndergraduateResearchAssistant,DepartmentofBiophysicsandBiochemistry,OregonStateUniversity,CorvallisOR;3UndergraduateResearchAssistant,DepartmentofMicrobiology,OregonStateUniversity,CorvallisOR;4*AssociateProfessor,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐4356
Thetimingoftheripeninginitiationisanimportanttraitforwinegrapeproduction.TheresearchprojectaimstovalidatetheregulatoryfunctionofARF4inthetimingofripeninginitiation.Theobjectivesareto:1)characterizeitsrolebyinducingorsilencingthegeneexpressioninthemicrovinesystemandtoidentifyitsinteractingproteinpartnersduringtheripeningprocess,2)toidentifyripening‐relatedgenestargetedbyARF4,and3)toevaluatefruitcompositionofberries,wherethetimingofripening‐initiationisaltered.Towardsthisgoal,weestablishedthemicrovinesatOSUandoptimizeddifferentstepsofproducingembryogeniccallithroughantherculture,inoculationofcalliwithagrobacteriumharboringthegenesofinterest,productionoftransformedembryos,transitioningoftheseembryostoplantlets,andfinallythetransferoftheplantletsinthegreenhouse.Weconductedourfirsttrialexperimentstoengineerthebiosynthesisofabscisicacid(hormonethatpromotesripening)usingthispipelineandweweresuccessfulinobtainingthetransformedembryos.Currently,thecloningofthedifferentplasmidconstructsaimedtoinduceandsilenceARF4inthemicrovineshasbeenperformed.TowardsidentifyingtheproteinpartnersofARF4,wefound170potentialcandidatesusingprotein‐proteininteractionscreeningassays.TheyincludeproteinsinvolvedinABAsignaling(VitViPAPA1),sugarsensing(VitviHXK1),andethylenesignaling(VitviETR4),allofwhichareknowntoinfluencefruitripening.Finally,weadaptedandtestedanewanalyticalmethodongrapeberryextractstomeasuremetabolitesassociatedwithorganicacids,aminoacids,phenolics,carbohydrates,polyols,andthreeclassesofflavonoids(anthocyanins,flavonols,monomeranddimeroftannins).
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TheEffectofYeastAssimilableNitrogenonSO2ProductionbySaccharomycescerevisiaeDuringWineFermentation
MaríaAlessandri1,AlanBakalinsky2*
1GraduateResearchAssistant,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR;2AssociateProfessor,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐6510
Sulfurdioxideiscommonlyusedduringwinemakingasanantimicrobialagentandantioxidant.However,highconcentrationscanleadtoproblematicfermentationsandpreventthemalolacticfermentationfromoccurring.Apartfromthesulfurdioxideadditionsmadebywinemakers,naturally‐occurringsulfitescanbeproducedbyyeastasanormalbyproductofmetabolism.Previousstudieshavefoundthatproductionvariesamongstrainsandisaffectedbyfermentationconditions.Insomecases,productionappearstobehighenoughtoreplaceorsignificantlyreducesulfiteadditionsbywinemakers.Thiscouldbeparticularlyrelevantfororganicwinemaking,wheresulfitesadditionsaredisallowedbyUSDAregulationsbutarepermittedintheEU.Wehavefoundthatnitrogenavailabilityingrapemustisonesourceofvariability.Ithasbeenpreviouslyreportedthatnitrogencontentingrapemusthasaneffectonyeastsulfurmetabolism,butuntilnowthefocushasbeenlargelyonH2Sproduction.Toourknowledge,asystematicstudyoftheeffectofnitrogenonsulfiteproductionhasnotbeenundertaken.Here,weanalyzedsulfiteproductionbydifferentyeaststrainsasafunctionofavailablenitrogen.Ourresultsindicatethathigherlevelsofnitrogencorrelatewithhighersulfiteproductionandthattheformofthenitrogenhasamajorimpact.Also,ourresultsshowthatcommercialnitrogensupplementsdonotaffectsulfiteproductioninaconsistentmanner.Thisinformationshouldbeofuseforwinemakersinterestedinproducinghighqualityorganicwines.
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ImpactofPre‐FermentationColdSoakConditionsonPinotNoirWineAromaCompounds
MengyingFu1,JaredJohnson2,JamesOsborne3,MichaelQian4*
1GraduateResearchAssistant,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR;2GraduateResearchAssistant,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR;3AssociateProfessor&EnologyExtensionSpecialist,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR;4Professor,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐9114
Thisstudyinvestigatedhowpre‐fermentationcoldsoakconditionsimpactedPinotnoirwinearoma.In2016,Pinotnoirwineswereproducedfromgrapesthatwerecoldsoakedforsixdaysattwodifferenttemperatures(6or10C)withtheadditionof0,50,or100mg/LSO2.Sixnon‐Saccharomycesyeastspecies,commonlyisolatedfromgrapes,wereaddedatthestartofcoldsoakandtheirpopulationsmonitored.Winewasalsoproducedfromgrapesthatdidnotundergocoldsoak.Attheendofcoldsoakthereweresignificantdifferencesinanumberofvolatilecompounds.Higherconcentrationsofethylacetateandisoamylacetatewerepresentincoldsoaksconductedat10Ccomparedto6C,whilehigherconcentrationsofphenylethylacetatewerepresentincoldsoaksconductedat10Cwith100mg/LSO2.Significantdifferenceswerealsofoundinthevolatilearomasofthefinishedwines,particularlyesters.InthesecondyearofthestudyanadditionofMetschnikowiafructicolawasmadeatthebeginningofasixdaycoldsoak.Attheendofcoldsoak,treatmentswithM.fructicolacontainedsignificantlyhigheralcoholsandlessacetateesters.Inthefinishedwinesthereweresignificantdifferencesinvolatilearomasbetweenwinesmadefromgrapesthatdidordidnotundergoacoldsoak.Pinotnoirwinesproducedwithoutcoldsoakhadsignificantlyhigherethylesterssuchasethylbutyrate,ethylisobutyrate,andethyloctonoate.WinesmadewithM.fructicolaalsocontainedsignificantlyhigherconcentrationsofbeta‐citronellol,ascomparedtothenocoldsoaktreatment.
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TheUseofSignalDetectionTheory(d')inFoodSensoryAnalysis
RachelHahn1*,ElizabethTomasino2,ClaudioFuentes3
1FacultyResearchAssistant,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR;2AssistantProfessor,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR;3AssociateProfessor,DepartmentofStatistics,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],503‐880‐2616
Signaldetectiontheory(oftendenotedbythesensitivityindexd`value)usesdifferencesinmeanstoquantifythedifferencebetweenastimulusofinterestandapossiblerandomdistractingpattern,usuallyintheformofa“CorrectRejection”or“FalseAlarm”application.Theuseofsignaldetectiontheoryoriginatesinpsychology,wheresubjectswereusedtodeterminea“threshold”offatigueafterextensiveexposuretovariousstimuli.Thedevelopmentofthisthresholdcoinedtheconceptof“signaltonoise”fordatacollectedbyunreliablesubjects(suchashumansoranimals).Theterm“signaltonoise”isusedtodescribetheratioofusabledatato“noise”orirrelevantdata,andisaformofthresholdanalysis.Thisthresholdanalysishelpsdeterminehowhumansubjectsareimpactedduringqualitativedatacollectionsessions.
Initiallythesensitivityindexwasdevelopedbypsychologists,buthassincebeenappliedtothefoodindustry‐specificallytosensoryscience.Formanyreasons,thesensitivityindexhasbecomeanindustrystandardforanalyzingfoodsensorydatabecauseitmeasuresandestimatesfatigueinfoodsensorysubjects.However,thereislittleliteraturerevisitingtheassumptionsofthesensitivityindexinthecontextoffoodsensoryscience.Thisresearchaimstorevisitthesensitivityindex’smathematicalexpressionandassumptionsinordertotailorittofoodsensoryresearch.
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InvestigatingFruitinessAromaPerceptioninWhiteWines
AngelicadeCastroIobbi1,ChaseJutzi2,ElizabethTomasino3*
1DoctoralCandidate,2UndergraduateResearchAssistant,3AssistantProfessor,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐4866
Winearomaiscomplexandtherearemanydifferentcompoundsthatcanberesponsibleforvariousaromas.Whitewinearomaischaracterizedbyimpactcompoundssuchasvolatilethiolsandthosethatproducearomasduetointeractionswithotherwinecomponents.Inthisstudyweinvestigatedthearomachemicalcomponentinteractionsinfluencingfruitinessperceptionofwhitewines.AneutralOregonPinotgriswinewasproducedandaromawasremovedbytheaddition1g/LLichrolut®EN.Acombinationofcompoundswasaddedtothewine,formingthearomabase.Thesearomabasecompoundsarepresentinallwines.Treatmentsinvestigatedarangeofdifferentchemicalcompoundssuchasesters,terpenes,alcohols,andthiols.Treatmentaromacompoundswereaddedtothebasewineatdifferentconcentrationsandcombinations.Overseveralsensorysessionstrainedpanelistsevaluatedthedifferentfruityaromasofthetreatmentwines.PanelistperformancewasdeterminedusingREMLandcanonicalvariateanalysiswasusedtorelatethewinechemicalcompositiontosensoryperception.Resultsshowarelationshipsbetweenterpenesandstonefruitaromas,andvolatilethiolsandtropicalfruitaromas.Otheraromacompoundsincombinationwithterpeneswerefoundtoalterthetypeoffruityaromas.Theresultsofthisworkwillhelpdevelopwinestylesandunderstandwhitewinequality.
2018OWRIGrapeDay Page18
EffectsofCropLevelonPinotNoirWineComposition
JingwenLi1,PatriciaA.Skinkis2,MichaelQian3*
1GraduateResearchAssistant,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR;2AssociateProfessor&ViticultureExtensionSpecialist,DepartmentofHorticulture,OregonStateUniversity,OregonWineResearchInstitute,CorvallisOR;3Professor,DepartmentofFoodScience&Technology,OregonStateUniversity,OregonWineResearchInstitute,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐9114
Todeterminetheimpactsofcroplevelonwinequality,aclusterthinningexperimentwasconductedinmorethan10vineyardsovera3‐yearperiod(2013–2015).Croplevelwerereducedbyclusterthinningusingaclusterpershootregime(2,1.5and1cluster/shoot)andcomparedtoafullcropcontrol(non‐thinned).Onesiteusedvariabletonperacretreatments(3.25,3,2.5,2,1.75ton/acre).Afterharvest,fruitfromfieldreplicateswerecombinedtoproduceonewineforeachtreatment.ThosewineswereanalyzedforvolatilecompositionbyGC‐MSandGC‐FIDintriplicate.Therelationshipsbetweencroplevelandconcentrationofquality‐importantcompoundsinwineweredeterminedbyregressionanalysis.Resultsshowedthatcropthinningpracticeaffectedsomevolatilecompositionsindifferentyears.Cropthinningleveldecreasedthecontentofmiddle‐chainacids(C6‐C10)in2013vintage.However,itincreasedthecontentofesters,terpinoids,alcoholsandmiddle‐chainacidsin2014vintage.Moreover,cropthinninglevelhavelimitedimpactonvolatilecompositionsin2015vintage.From2013to2015vintage,thecontentofvolatilecompositionsinwineincreased.Combiningallthreeyears’datawithoutconsideringyeardifference,itwasfoundthatthecontentofestershadincreasingtrendswhileterpinoids,alcohols,middle‐chainacidsandvolatilephenoliccompoundshadnodifferencewithhighercropthinninglevel.
2018OWRIGrapeDay Page19
Useofnon‐SaccharomycesYeasttoReduceVolatileAcidityProductionDuringColdSoakingofPinotNoirGrapes
JaredJohnson1,MengyingFu2,MichaelQian3,JamesOsborne4*
1GraduateResearchAssistant;2GraduateResearchAssistant;3Professor,DepartmentofFoodScience&Technology,OregonStateUniversity,OregonWineResearchInstitute,CorvallisOR;4AssociateProfessor,DepartmentofFoodScience&Technology,OregonStateUniversity,OregonWineResearchInstitute,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐6494
Coldsoakingisatechniqueusedduringredwinemakingtoalterthearoma,flavor,andphenoliccontentofawine.However,coldsoakingdoesnotcomewithoutriskasthisprocesscanresultintheproductionofspoilagecompoundssuchasaceticacidandethylacetateifexcessivegrowthofmicroorganismssuchasHanseniasporauvarum(Kloeckeraapiculata)occurs.H.uvarumgrowthduringcoldsoakistypicallycontrolledthroughtheuseofsulfurdioxideandmaintaininggrapesatacoldtemperature.Whileeffective,thesemethodscanalsorestrictthegrowthofothernon‐Saccharomycesyeastpresentduringcoldsoakthatcancontributepositivelytowinearoma.Recently,theadditionofselectnon‐Saccharomycesyeastatthebeginningofcoldsoakhasbeensuggestedasanalternativemethodforreducingvolatileacidity.Thisstudyinvestigatedtheefficacyofselectnon‐SaccharomycesyeaststrainsforthereductionofvolatileacidityandH.uvarumgrowthduringcoldsoak.Commercialnon‐SaccharomycesyeastswerescreenedfortheirabilitytoreduceH.uvarumgrowthandaceticacidproductionduringasimulatedcoldsoakinagrapejuicebasedmedium.H.uvarumgrowthandaceticacidproductionwasreducedinthepresenceofallnon‐SaccharomycesyeasttestedwithsomeyeastreducingH.uvarumgrowthandaceticacidproductiontoagreaterextent.Oneyeast,Metschnikowiafructicola,wasthenusedinPinotnoirwinemakingexperiments.PinotnoirgrapeswereinoculatedwithacombinationofH.uvarumandM.fructicolaandcoldsoakedforsixdays.DuringcoldsoakM.fructicolareducedH.uvarumgrowthandsignificantlydecreasedaceticacidandethylacetateproduction.Theseresultssuggestthattheadditionofnon‐SaccharomycesyeastduringcoldsoakmaybeaneffectivemethodforreducingtheproductionofvolatileaciditybyH.uvarum.
2018OWRIGrapeDay Page20
InfluencesofYeastProductAdditionandFermentationTemperatureonChangesinLipidCompositionsofPinotNoirWines
QuynhPhan1,JamesOsborne2,ElizabethTomasino3*
1DoctoralCandidate;2AssociateProfessor;3AssistantProfessor,DepartmentofFoodScience&Technology,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐4866
Concentrationandcompositionoflipidspresentinwinehavebeenshowntobedependentonyeastandgrapevariety.Therearetwosourcesoflipidsinwine:firmtissuesofgrapesandalcoholicfermentationbyyeast.Thetemperatureofawinefermentationalsoaffectslipidmetabolism;therefore,lipidprofilesvaryunderdifferentwinemakingprocesses.ThisstudyinvestigatedhowdifferenttypesandamountofyeastderivativeproductsaddedwouldaffectthelipidcontentofPinotnoirwines.Changesinlipidcompositioninwinesaccordingtofermentationtemperaturewerealsoexamined.The2017OregonPinotNoirgrapeswerefermentedat8Cand27C.Afterprimaryandmalolacticfermentation,yeastproductsAutoleesandOenolees(Laffort,USA)wereaddedtothewinesfor60daysasseparatetreatmentsofAutolees(0.3g/L,0.175g/L,and0.05g/L)andOenolees(0.4g/L,0.3g/L,and0.2g/L)orasamixtureofAutolees(0.3g/L)andOenelees(0.4g/L).Liquid‐liquidextractionmethodwithchloroform/methanol(2:1v/v)asthesolventwasusedtoextracttotallipidsintheexperimentalwines.Thelipidsextractedwereclassifiedaspolarlipids(PL),sterols(ST),freefattyacids(FFA),triglycerides(TG),andcholesterolester(CE)bythin‐layerchromatography.Thefattyacidsderivatives,fattyacidmethylesters(FAME),wereanalyzedbygaschromatographymassspectrometry(GCMS).Theresultsofthisstudycontributetotheunderstandingofhowdifferencesinlipidcompositioncouldbeusefulfordeterminingwinestyleandwinequality.Fattyacidcompositionandthepolyunsaturatedfattyacid(PUFA)ration‐6/n‐3couldbeusedtoevaluatethequalityoflipidsinwine.Infutureresearch,itwouldsubstantiallycontributetotheinvestigationoftheinteractionsoflipidswithotherwinecomponentssuchastanninandpolysaccharideaffectingthesensorypropertiesofwinesuchastasteandmouthfeel.
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ComparativeAnalysesoftheTotalCarbohydrateCompositionofRedWinePolysaccharides
LudwigRing1,ElizabethTomasino2,JamesOsborne3,MichaelQian4*
1ResearchAssociate;2AssistantProfessor;3AssociateProfessorandEnologyExtensionSpecialist;4Professor,DepartmentofFoodScience&Technology,OregonStateUniversity,OregonWineResearchInstitute,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐9114
Thechemicalnatureofredwinemouthfeelhasbeenaddressedinmultiplerecentstudies.However,theirvastmajorityfocusedonpolyphenolssuchastannins,whichhavebeenshowntoaccountfortheastringencyofredwine.Incontrast,thecompoundscontributingtomouthfulnessandbodyremainunclear.Thisongoingstudyinvestigatesredwinepolysaccharides,sincetheymightaffectthetexturalsensationandthusthemouthfeelpropertiesofredwine.Aprocedureincludingprecipitation,hydrolysis,andderivatization(silylationwithTMSI)hasbeendevelopedtoanalyzethetotalcarbohydratecompositionbymeansoftheirper‐O‐trimethylsilylatedmethylglycosidederivativesviaGC‐FID.Variationsinthetotalcarbohydratecompositionofseveralredwinescouldbeobserved.Althoughallsamplescomprisedthesamemonomers(mannose,arabinose,galactose,rhamnose,galacturonicacid,glucose,andxylose),theratiosdiffered.Dependingonthemonomer,thiscangiveinsightsintothewinemakingprocess.Forinstance,ahighmannoseportioncouldresultfromalongeryeastcontact,sincemannoproteinsoriginatefromyeastcellwalls.However,itremainstobeinvestigatedwhichparametersmostinfluencethecomposition(e.g.winemakingprocess,grapevariety,age),andtowhichextentthisaffectsthemouthfeel.Additionalsensorystudieswillexaminetheorganolepticimpactoftheredwinepolymerstoelucidatepotentialcorrelationsbetweenanalyticalandsensorydata,inordertogetabetterunderstandingofthenon‐volatilesensoryactivecompoundsinredwine.
2018OWRIGrapeDay Page22
FieldObservationsofTortistilusSpeciesAssociatedwithOregonVineyards
DanielT.Dalton1*,RichardJ.Hilton2,VaughnM.Walton3
1GraduateResearchAssistant,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;2SeniorFacultyResearchAssistant,SouthernOregonResearch&ExtensionCenter,OregonStateUniversity,CentralPointOR;3Professor,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐3913
FieldcollectionsofcandidateinsectvectorspeciesofGrapevineredblotchvirus(GRBV)wereacquiredinOregonin2017,andbiologicalattributesofthesespeciesweredocumented.Treehopperinsects(Hemiptera:Membracidae)ofapreviouslyconfirmedvectorspecies,Spissistilusfestinus,werefoundnearvineyardsinsouthernOregon.TwomorphospeciesofthetreehoppergenusTortistiluswerefoundinandaroundWillametteValleyandsouthernOregonvineyardswherevirusspreadwasobserved.Tortistilusspeciesoverwinteraseggsthatarelaidinnewstemsorbehindthebudsofwoodyperennialplantsincludingoak,apple,pear,hawthorn,andgrapevine.Eggshatchinspring,andearlyinstarsfallfromwoodyhostsontolushherbaceousunderstoryplantssuchasvetch.Late‐instarinsectsmigratetodrought‐hardyplantspeciesincludingwildcarrotastheunderstoryvegetationdriesoutinearlysummer.FifthinstarinsectsmoltintoadultsbeginninginJuly.Eggsaredepositedintowoodyhoststocompletetheinsectlifecycle.Adultinsectsdonotappeartomigrategreatdistancesbutarefoundalongvineyardedgesandinunmanagedhabitatsinsummerandfall.
Acombinationofcollectiontechniquesincludingvacuumsampling,sweepnetting,stickytrapmonitoring,visualsurveying,andhandsamplingwasusedtodeterminetheinsectlifecycleandbehavioralpatterns.Feedingbytreehoppersmaygirdleyoung,growingstemtissues.Inred‐fruitedwinegrapecultivars,extremereddeningoftissueabovegirdledareasisusuallyapparent,allowinggrowerstofindinsectsandtrackpotentiallyaffectedplants.
TestedinsectsshowedpersistenceofGRBVforatleastfiveweeksfollowingacquisitionofthevirusingreenhousetransmissiontrials.ActualtransmissionofGRBVbyTortistilusspeciesremainstobeconfirmedingreenhousebioassays.
2018OWRIGrapeDay Page23
DeficitIrrigationandGrapevineRedBlotchDiseaseManagement
A.N.KC1*,A.L.Rasmussen2,A.Levin3
1AssistantProfessor,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;2FacultyResearchAssistant,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;3AssistantProfessor,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR
*CorrespondingAuthor:[email protected],541‐772‐5165
GrapevineRedBlotchDisease(GRBD)isanewlyidentifieddiseaseofgrapevinescausedbygrapevineredblotchvirus(GRBV).Theeconomiclossassociatedwiththediseaseisreductioninfruitqualityanddelayedripening.Theimpositionofmoderatewaterdeficitsisacommonviticulturalpracticetoadvanceripeningandimprovefruitquality.However,thestressexperiencedbyvinesunderdeficitirrigationcanpotentiallyamplifythenegativeeffectsofGRBD.ResearchwasconductedtounderstandinteractionbetweenGRBVanddeficitirrigationondiseasedevelopmentandfruitquality.Asplit‐plotfieldexperimentwithtwoirrigationtreatments,wetanddry;andtwovinestatuses,healthyandinfected,werearrangedinarandomizedcompleteblockdesign.Irrigationtreatmentswereimposedbyvaryingthenumberofdripemitterspervinewherewetanddrytreatmentswereirrigatedat100and66%ofcropevapotranspiration(ETc)respectively.Inlinewatermeterswereusedtoquantifyappliedwateramounts.VinehealthstatuswasdeterminedbyPCR‐basedassaysasinfectedornon‐infectedwithGRBV.Diseaseseveritywasrecordedeveryweekafterthefirstsymptomappearanceoninfectedvines.Nosignificantinteractionbetweenwaterandvinehealthstatustodiseaseseveritywasobserved.However,thestemwaterpotentialofinfectedvineswassignificantlyhighercomparedtohealthyvinesinbothdryandwettreatments.Inaddition,overallfruitqualityoftheinfectedvineswasbetterinwetirrigationtreatmentthanthedrytreatment.Theresultssuggestthatkeepingvineswell‐wateredmaymitigatesomeofthenegativeeffectsofGRBVinfection.
2018OWRIGrapeDay Page24
InteractionofDeficitIrrigationandGrapevineRedBlotchVirus(GRBV)onDiseaseDevelopmentandGrapevinePhysiology
AlexanderLevin1*,ChristopherJenkins2,JudyChiginsky3,AnnRasmussen4,AchalaKC5
1AssistantProfessor,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;2FacultyResearchAssistant,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;3BiologicalSciencesResearchTechnicianII,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;4FacultyResearchAssistant,DepartmentofBotanyandPlantPathology,OregonStateUniversity,CorvallisOR;5AssistantProfessor,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR
*CorrespondingAuthor:[email protected],541‐772‐5165
Whilemoderatewaterdeficitsadvanceripeningandimprovefruitqualityinhealthygrapevines,theycanpotentiallyamplifynegativeeffectsofviraldiseaseinGRBV‐infectedgrapevines.Therefore,afieldexperimentwithtwoirrigationtreatments–wetanddry–andtwodiseasestatuses–healthy(RB‐)andinfected(RB+)–wasinitiatedtounderstandtheinteractionbetweenGRBVinfectionanddeficitirrigation.Wetvineswereirrigatedat100%ofcropevapotranspiration(ETc),whiledryvinesreceivedwaterat66%ETc.HealthyandinfectedvineswereconfirmedbyPCR‐basedassays.DiseaseprogressionandseveritywererecordedweeklyafterfirstsymptomswereobservedonRB+vines,andvinewaterstatus(Ψstem)wasregularlymonitoredthroughoutthegrowingseason.Atharvest,yieldandyieldcomponentsweredetermined,andberrysampleswerecollectedforcompositionalanalyses.Therewasnosignificantinteractionbetweenirrigationtreatmentanddiseasestatusondiseaseprogressionandseverity.Pre‐veraisonΨstemwasnotaffectedbydiseasestatusbutwassignificantlyhigherinRB+vinespost‐veraison.ThehigherΨsteminRB+vinesresultedinlargerberriesandyieldatharvest,butfewofthedifferencesinyieldandyieldcomponentsamongtreatmentsweresignificant.Berryflavonoidsweremorestronglyaffectedbydiseasestatuscomparedtosugarsandacids,withlittleeffectofirrigationtreatments.Inskinsandseeds,significantdifferencesamongtreatmentswereobservedinconcentrationandcontentofanthocyaninsandiron‐reactivephenolics(IRPs),butnottannins.SmalldifferencesintanninscoupledwithlargedifferencesinIRPssuggeststhatGRBVstronglyinhibitedbiosynthesisofnon‐tanninIRPs,particularlyinseeds.Takentogether,theseresultssuggestthatkeepingvineswell‐wateredmaymitigatesomeofthenegativeeffectsofGRBV,butultimatechangesinsecondarymetabolismduetoGRBVinfectionmaynecessitateusinginfectedfruitfordifferentwineprogramsorblendingwithlotsfromhealthyvineyards.
2018OWRIGrapeDay Page25
ExogenousApplicationofAbscisicAcid(s‐ABA)DoesNotImproveFruitCompositioninRedBlotch‐InfectedGrapevines
VaughnWalton1,AlexanderLevin2*,DanielDalton3,ChristopherJenkins4,JudyChiginsky5,AchalaKC6,AnnRasmussen7
1Professor,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;2AssistantProfessor,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;3GraduateResearchAssistant,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;4FacultyResearchAssistant,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;5BiologicalSciencesResearchTechnicianII,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;6AssistantProfessor,SouthernOregonResearchandExtensionCenter,OregonStateUniversity,CentralPointOR;7FacultyResearchAssistant,DepartmentofBotanyandPlantPathology,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐772‐5165
Plantgrowthregulatorsarecommonlyusedtoimproveripeningandberrycompositioninhealthygrapevines.Recently,ithasbeensuggestedthatgrapevineredblotchvirus(GRBV)disruptsthenormalhormonalsignalinginvolvedinripeningonsetinberries.Sinceendogenouslyproducedabscisicacid(s‐ABA)playsalargeroleinberryripening,itispossiblethatexogenousapplicationsofs‐ABAcouldmitigatethedeleteriouseffectsofGRBV.Therefore,theeffectsofexogenouss‐ABAapplicationonfruitripeninginGRBV‐infectedgrapevinesweretestedintwoOregonAVAscharacterizedbydifferentclimates:theWillametteValley(WV;coolandwet)andtheRogueValley(RV;warmanddry).Ateachsite,candidatevineswereidentifiedbasedonthepreviousyear’ssymptomologydataandconfirmedforGRBVinfectionwithPCR‐basedassays.Cluster‐directedsprayapplicationsofs‐ABAweremadeat50%veraisonand10‐14dayslateratarateof300mg/L.Experimentaltreatmentswerea2x2factorialcombinationofdiseasecondition–healthy(RB‐)andinfected(RB+)–ands‐ABAapplication–spray(ABA+)ornospray(ABA‐).Atharvest,herewerenosignificanteffectsofs‐ABAonberryfreshweight,totalsolublesolids(TSS),pH,ortitratableaciditywithinRB+orRB‐vines.TherewerealsonosignificanteffectsofdiseasestatusontheaforementionedparametersinWVvines,andinRVvines,onlyTSSwassignificantlyhigherinRB‐vines.Therewerenosignificantinteractionsbetweens‐ABAapplicationanddiseasestatuswithrespecttopolyphenoliccompositioninskinsandseeds.Surprisingly,ABA+vineshadlowerconcentrationsoftanninsandiron‐reactivephenolicsacrosseitherdiseasestatusintheskins,butsincetherewereminimaleffectsintheseeds,therewerenon‐significanttreatmenteffectswithrespecttototalconcentration.Overall,theexogenousapplicationofs‐ABAatveraisondidnotimprovefruitcompositionacrosstwodistinctgrowingregionsatharvest.
2018OWRIGrapeDay Page26
ExploringtheSymptomologyandImpactsofRedBlotchDiseaseonPinotNoir
JustinLitwin1,PatriciaA.Skinkis2*,RobertMartin3
1UndergraduateResearchAssistant,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;2*AssociateProfessor&ViticultureExtensionSpecialist,DepartmentofHorticulture,OregonStateUniversity,CorvallisOR;3ResearchPlantPathologist,USDA‐ARS,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐1411
In2012anewvirus,GrapevineRedBlotchassociatedVirus(GRBaV),wasidentifiedingrapevinesandhasthepotentialtoreducefruitquality.TodeterminetheeffectofthevirusinOregon’sWillametteValley,astudywasconductedtomonitoranddocumentvisualsymptomsofGRBaVincoolclimatePinotnoirandidentifytheimpactsonvinephysiologyandfruitquality.AcommercialPinotnoirvineyardintheEola‐AmityHillsAVAwasmonitoredfrompea‐sizestageofberrydevelopmentthroughleaffallin2017.Vinesweremonitoredbasedontheirvirusstatus(positiveornegative)andsymptoms(presentorasymptomatic).Visualobservationsofsymptomsandchlorophyllmeasuresweremadeweeklyandphotoassimilationandstomatalconductanceweretakenwhenweatherconditionsallowed.Visualsymptomsinthecanopydidnotoccuruntilafter100%veraisonandbeganwithbasalleafreddeningthenprogressedapicallyastheseasoncontinued.Leaffalloccurredlaterinvinesthatshowedvirussymptoms.AllvineswithcanopysymptomsalsotestedpositiveforGRBaV,butnotallvinesthattestedpositiveshowedsymptoms.Thiscouldindicateadelayedsymptomaticexpressionofthevirus.Thechlorophyllmeasurestakenthroughtheseasonshowednodifferencebetweensymptomaticandasymptomaticvines.GRBaVpositivevineshadlowerstomatalconductanceandphotoassimilationingeneralbutwasonlystatisticallysignificantduringtwodates.NutrientsamplescollectedduringveraisonshowedonlylowerleafphosphorusinGRBaVpositivevines,whilesymptomaticvineshadlowerpotassiumandlowerpetioleCa,Mg,Zn,andMn.Yieldandbasicripenessdidnotdifferatharvest.Virusstatusdidnotaffectberryconcentrationsoftotalanthocyanin,tanninandphenolics.However,symptomaticvineshadlowertotalphenolicconcentrations.Thesamevinesaretobemonitoredin2018togainafurtherunderstandingofthephysiologicalimpactsofthedisease.
2018OWRIGrapeDay Page27
RapidSamplingTechniquestoDetermineQoIFungicideResistanceinErysiphenecator
SarahR.Lowder1*,TaraNeill2,MichelleM.Moyer3,TimothyD.Miles4,WalterF.Mahaffee51GraduateResearchAssistant,DepartmentofBotanyandPlantPathology,OregonStateUniversity,CorvallisOR;2BiologicalScienceTechnician,USDA‐ARS‐HCRU,CorvallisOR;3AssociateProfessor&StatewideViticultureExtension,WashingtonStateUniversity,ProsserWA;4AssistantProfessor,DepartmentofPlant,SoilandMicrobialSciences,MichiganStateUniversity,EastLansingMI;5ResearchPlantPathologist,USDA‐ARS‐HCRU,CorvallisOR
*CorrespondingAuthor:[email protected],704‐574‐4285
Controlfailuresofgrapepowderymildew(causedbyErysiphenecator)aftertheuseofquinoneoutsideinhibitor(QoI;FRAC11)fungicideshavebeenreportedthroughoutOR,WA,andCA.Quick,cost‐effectivemethodstodetectfungicideresistanceinE.necatorarenecessarytoreducetheselectionforresistantisolatesandmitigateineffectivefungicideapplications.In2017,E.necatorsampleswerecollectedvialinearstratifiedsamplingtechniquesbyplacingaToughSpotadhesivedotdirectlyontoamildewcolony(n=119)orbywipinggloveswithsterilecottonswabs(n=65)aftersimulatedcanopymanagement.AcompetitiveTaqManqPCRassay,previouslydemonstratedtobein100%agreementwithfungicideresistancescreeningbioassays,wasusedtodetecttheG143AmutationinthecytbgenethatisassociatedwithQoIresistance.Bothmethodsdetectsimilardistributionsinresistancestatus:ToughSpots‐48.7%resistant,43.7%sensitive,7.6%mixed;Swabs‐59.2%resistant,24.5%sensitive,16.3%mixed.Thisindicatesthateithermethodcouldbeaviablerapidsamplingtechniquewithminimalinterruptiontoproductionschedules.Arapidsamplingtechniqueforchasmothecia(syn.cleistothecia)overwinteringingrapebarkisbeingdevelopedtoprovidemanagerswithapre‐seasonassessmentoftheriskoffungicideresistanceforthegrowingseasonfollowingsampling.Thewild‐typeandresistantgenotypescanbedeterminedfromasinglechasmotheciuminabackgroundof0.2gofdrybark.Reducingthetimeandcostsrequiredtoassessfungicideresistancewillallowvineyardmanagerstomakeinformedchoiceswhendeterminingfungicideproductselectionandrotation.
2018OWRIGrapeDay Page28
CleanGrapevines–TowardaHarmonizedGrapevineCertificationProgramforthePacificNorthwest
RobertR.Martin1*,ResearchLeaderandResearchPlantPathologist,USDA‐ARS‐HCRU,CorvallisOR
*CorrespondingAuthor:[email protected],541‐738‐4041
Morethan70virusandvirus‐likeagentsareknowntoinfectgrapevinesworldwideandrelativelyfewareofmajorimportanceinthePacificNorthwest.Thisisdueinalargeparttoquarantineandcertificationprogramsthathaveresultedineliminationofmostofthesevirusesfromplantingmaterials.Onceidentified,GrapevineRedBlotchVirus(GRBV)wasaddedtothelistofpathogensinmostcertificationprograms.CertificationprogramsaremanagedbyStateDepartmentsofAgricultureandthereforeeachstatemayhavedifferentrequirementsthatnurseriesneedtomeettoproduce‘certifiedgrapevines’.Thus,certificationcanmeandifferentthingsdependingonwheretheplantswereproduced.OverthepasttwoyearstherehasbeenanefforttoharmonizethegrapevinecertificationprogramsandquarantinesinWashington,OregonandIdaho.ThiseffortisfundedbyagrantfromUSDA‐APHISandledbyVickyScharlau,ExecutiveDirector‐WashingtonWineIndustryFoundation.TheprojectincludesregulatorystafffromthethreeStateDepartmentsofAgriculture,industrymembers,nurseriesandscientists.TheprocessisnearcompletionandthereshouldbeacommongrapevinecertificationprogramforthePacificNorthwestinthenearfuture.Thereareseveralcaveatsthatgrowersneedtobeawareof:1.ThenewcertificationprogramwillrequireallgrapevinescomingintothethreestatesbefromcertificationprogramsrecognizedbytheStateDepartmentofAgriculture;2.Certificationisvoluntaryandthereforenotallnurseriesaresellingcertifiedgrapevines;3.Theharmonizedcertificationprogramsdonotrequirethatgrapevinessoldin‐statearefromcertifiednurseries;and4.Forgraftedgrapevinestherootstockandscionmustbecertifiedfortheplanttobeconsideredcertified,forexample,certifiedrootstockbroughtintothestateandgraftedwithuncertifiedscionwooddoesnotresultinacertifiedplant.
2018OWRIGrapeDay Page29
ARegionalMildewManagementProblem‐FungicideResistanceintheWest
TaraNeill1*,SarahR.Lowder2,TimothyD.Miles3,WaltMahaffee4,MichelleMoyer5,MonicaCooper6
1BiologicalScienceTechnician,USDA‐ARS‐HCRU,CorvallisOR;2GraduateResearchAssistant,DepartmentofBotanyandPlantPathology,OregonStateUniversity,CorvallisOR;3AssistantProfessor,DepartmentofPlant,SoilandMicrobialSciences,MichiganStateUniversity,EastLansingMI;4ResearchPlantPathologist,USDA‐ARS‐HCRU,CorvallisOR;5AssociateProfessor&StatewideViticultureExtension,WashingtonStateUniversity,ProsserWA;6FarmAdviser‐Viticulture,UniversityofCaliforniaCooperativeExtension,NapaCA
*CorrespondingAuthor:[email protected],541‐738‐4028
Throughout2017,afieldscoutingcampaignthatcoveredCentralandNorthernCalifornia,WesternOregon,andSouthernWashingtonyieldedover850fieldsamplesand64isolatesofErysiphenecator.Analysisofthesesamplesforresistancetoquinoneoutsideinhibitor(QoI)fungicides(FRACGroup11) usingtheG143AqPCRassayindicatedwidespreadresistancethroughoutallgrapegrowingregionsscouted(93%containedresistantE.necatoramongToughSpotkitsamples);withhigherfrequenciesinWashington(96%)andCalifornia(93%)thaninOregon(47%).Analysisof2017E.necatorsingle‐chainisolatesgeneratedsimilarresultswith90%ofWashington,94%ofCalifornia,and69%ofOregonisolatesshowingQoIresistance.TheseresultswereconfirmedwhenisolateandfieldsampleDNAunderwentgenotypingbysequencinganalysisofthecytbgene.TheseresultsaresimilartotheQoIresistanceobservedthroughoutOregonin2015and2016.Analysisofairsamplescollectedusingimpactionsporesamplersfromcommercialvineyardsfrom2013‐2017showedthatQoIresistancewasdetected2yearspriortocontrolfailuresin2015andthefrequencyoftheG143Aallelepeakedin2016anddecreasedin2017.AirsamplescollectedfromWashingtonvineyardsduringthe2017growingseasonshowedaveryhighfrequencyoftheG143Aallelewith93%ofsamplescontainingit.Analysisofthesesamplesandisolatesusingvariousmoleculartechniquesandfungicideresistancebioassaystodetermineresistancetodemethylationinhibitor(DMI)fungicides(FRACgroup3) andsuccinatedehydrogenaseinhibitor(SDHI)fungicides(FRACgroup7)isongoing.AqPCRassaywasdevelopedtotargetapointmutation(Y136F)oftheCYP51genethatisacontributingfactortoDMIresistanceinE.necator;themutationispresentin85%oftested2017isolates(n=60)and96%of2015and2016isolates(n=92).
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EarlyBloomTimingforBetterGrapePowderyMildewManagementinWesternOregon
JayW.Pscheidt1*,JohnBassinette2
1Professor;2FacultyResearchAssistant,DepartmentofBotanyandPlantPathology,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐5539
Fungicidesefficacytrialsforgrapepowderymildew(Erysiphenecator)managementhavebeenconductedattheBotanyandPlantPathologyFieldLaboratory,Corvallis,ORsince1990.Vineyards,withanyoffivecultivars,werecaneorspurprunedwithverticalshootpositioningandmanagedforweedsandinsects.Weatherconditionswerefavorableeachyearforintensepowderymildewdevelopment.Fungicideswereappliedusingahoodedboomsprayerwhereeachsprayprogramwasreplicatedonfourorfivesetsoffivevineseach.Fungicideprogramswereinitiatedwhenthefirstpowderymildewsymptomswereobservedandthencontinuedattwoweekintervalsthroughtheseason.Theincidenceandseverityofpowderymildewonfruitwasevaluatedvisuallyatveraisonbyarbitrarilyselecting50clustersforexaminationfromthemiddlethreevinesofeachreplicate.Datafrom31fungicidetrialsconductedbetween1990and2017wereusedforanalysis.Thefungicideprogramwiththelowestpowderymildewseverityineachtrialwasselectedforcomparisonwithothertrials.Programsselectedconsistedofeitherasingleformulation(containingoneortwoFRACgroups)usedthroughouttheseasonormultipleformulations(eachcontainingoneortwoFRACgroups)alternatedthroughouttheseason.Allfungicideprogramswereinitiatedpriortobloomforeachcultivar.Attwoweekintervals,bloomapplicationsoccurredanywherefrom10%(BBCH61)to90%bloom(BBCH69)withtwoprogramsmissingbloomaltogether.Ingeneral,incidenceorseverityofpowderymildewwaslowerifseason‐longprogramsincludedanapplicationat10%to40%bloom.Incidenceorseverityofpowderymildewtendedtobehigherandwithgreatervariationifseasonlongprogramsincludedanapplicationatlaterbloomstages.GrowersinwesternOregonareadvisedtoadjustfungicidemanagementprogramstoincludeanapplicationatearlybloomstages.
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AStepTowardsMoreSustainableVineHealth:theCleanPlantApproachtoGrapeVirusDiseaseManagement
NeilMcRoberts1*,KamyarAram2,KariArnold3,DeborahGolino4
1AssociateProfessor,PlantPathologyDepartment,UniversityofCalifornia,DavisCA;PostDoctoralResearcher,PlantPathologyDepartment,UniversityofCalifornia,DavisCA;3FarmAdviser,UniversityofCaliforniaCooperativeExtension,ModestoCA;4CooperativeExtensionSpecialist,Director,FoundationPlantServices,UniversityofCalifornia,DavisCA
*Correspondingauthor:[email protected]‐752‐3248
Manythingschipawayattheproductivityandprofitabilityofgrapevines,andsincethebulkoftheeconomicproductionfromwinegrapestypicallycomesafter10yearspost‐planting,itisimportanttoprolongthehealthylifespanofvineswellintotheirsecondorthirddecade.Manygrapevirusesareknown,causingarangeofdiseasesthatvaryinseverityinthecontextofwineproduction.OverthelasttwodecadesGrapeLeafRollassociatedVirus3(GLRaV‐3)andGrapeRedBlotchVirus(GRBV),havebeenofparticularconcerninCalifornia,andthesourceofconsiderableanxietyaboutthevalueandoperationofthegrapecertificationsystem.In2011westartedaresearchandextensionefforttounderstandthedynamicsofvectoredvirusesingrapeproductionsystemsinCaliforniaandtoprovideinformationtogrowers,nurseriesandwinemakers,onhowtoimprovethevoluntarygrapecertificationandregulationscheme.Thegrapeproductionqualitychainischaracterizedbyseveralpressurepointswheretrustandcooperationamongparticipantsiskeytoachievinglong‐term,sustainablesuccess.Thetalkwilloutlinewhatthosepressurepointsare,whytheymatter,andwhatstepsgrapegrowerscantaketomanagethemincooperationwithotheractorsinthequalitychain.Thekeymessageisthatthereisnozero‐cost,simplesolution.Giventhat,thebestwaytoinvesttimeandmoneyisinstartingthelifeofavineyardwiththecleanestplantingmaterialpossible,andthencooperatingwithothergrowersinneighborhoodstomanagevirusesandtheirvectorsdowntoaslowaslevelaspossible.Virusdiseasemanagementbuiltaroundalong‐termcooperativestrategyissusceptibletoallthewell‐knownreasonsthatcooperation‐dependentactivitiesmightfail;knowingwhatthebestapproachis,doesnotnecessarilymakeiteasytoadopt.
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AreVineyardBuyersWarmingUptoCoolerLocations?QuantifyingtheValueofLocationSpecificAdaptability
W.JasonBeasley1*,RobinCross2
1GraduateResearchAssistant;2AssistantProfessorandSeniorResearcher,DepartmentofAppliedEconomics,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐2942
Itiscommonlyacceptedthatavineyard’slocalclimateisanimportantattributeforgrapequality.Eventhoughvineyardsmaypersistfordecades,thereisgreatuncertaintyintheexactchangeinclimateexpectedforanygivenlocation.Instead,prospectivevineyardownersmustevaluatecurrentclimatesandassumefuturesuitabilityinanylocation.Whatmightinfluencetheperceptionsurroundingtheuncertaintyinanticipatedclimatechanges?Wewillshowthatoneperceptionshifteristhemedia.Coverageofclimatechangefromwell‐knownprintnewsoutletswillaffectbeliefspertainingtoclimatechange.Ifthemediadoesshiftperceptionaswewillshow,towhatextentdoourbeliefsinfluenceourreal‐timedecisions?Weuseanewlycompileddataset,comprisedofvineyardattributesandsalesinformationformuchofOregonandCalifornia.Wecombinethisdatawithmeasuresofmediacoverageonclimatechangeandsurveyresultspertainingtotheimportanceofclimatechange.Resultssuggestthatgreatermediacoverageofclimatechangeisstrongenoughtoinfluencevaluesincoolerregions.Thatis,vineyardsthatarecoolerthanaverageinCaliforniaandtheentirestateofOregon,haveapremiumthatincreaseswiththestrengthofperceptionsonclimatechange.Theseresultsmaysuggestthatadaptationtoclimatechangeisalreadyoccurring.Thisalsosuggeststhatmediacoveragemayinfluencethetimingofanorthernmigrationofcapital,asallofOregonvineyardscommandacoolerpremium.Additionally,weexplorehowlargethesepremiumsmaybeacrossregionsandhowfutureforecastsinfluencepricing.
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ValuingOregon’sWineGrowingRegions
RobinM.Cross1*
1AssistantProfessorandSeniorResearcher,DepartmentofAppliedEconomics,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐1397
Vineyardownerscannowreceiveataxbreakinproportiontothereputation(brand)valueoftheirwinegrowingregion.TheIRSrequiresevidenceforthededuction,leadingtothequestion:Whodecidesreputation’svalue?
Reputationisacollectionofcontributionsfromgrapegrowers,winemakers,expertreviewers,andconsumers,togetherwitharegion’sgeography,climate,andsoil.EventhefederalgovernmentplaysarolebygrantingAmericanViticulturalArea(AVA)statustoregionsdemonstratingdistinctivegeo‐climaticcharacteristics.
Appraisersandstatisticianshavetriedtoseparateandvaluetheseinterrelatedcontributionsacrossmanyoftheworld’sgreatestwineproducingregions.But,interrelatednessconfoundsbothhumanandstatisticalmethods,loweringconfidenceanddepressingreportedvalues.
Fortunately,FrenchmathematicianLaplaceproposedasolutiontotheinterrelatednessproblemin1816,shortlyafterLegendre’sintroductionofthemostenduringstatisticalmodel‐linearregression.Wederivethesolution’sstatisticalpropertiesanduseittorevisitpastresearchonOregon’svineyardvalues.Weexplorehowinterrelatednessdisruptedpreviousfindings,loweredestimates,andlimitedthebreadthoftestablequestions.Wethenseparateandanalyzetheinfluencesofgeo‐climaticcharacteristics,federalrecognition,andtheevolutionofreputationovertime.
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UsingeconomicanalysistoInformBusinessDecisions–ExamplesfortheOregonWineIndustry
CatherineDurham1,JamesSterns2*
1AssociateProfessor,DepartmentofAppliedEconomics,FoodInnovationCenter,OregonStateUniversity,PortlandOR;2AssociateProfessor,DepartmentofAppliedEconomics,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐1406
Withinthepast15months,theOregonWineResearchInstitute(OWRI)hastakenstepstoestablish“economicandbusinessresearch”asaprioritycommitmentonparwiththeOWRI’shistoricsupportforviticultureandenologicalresearch.Earlyinitiativesincludedastate‐widelisteningtourtogatherindustryperspectivesaboutpriorityresearchtopicsrelatedtothebusinessandeconomicsofthewineindustry.Baseduponfindingsfromthistour,tworesearchprogramsrelatedtoconsumerdemandandpurchasingbehaviorarebeingimplemented.Asresourceshavebecomeavailable,otherissuesofimportancetotheindustryhavebeenaddedtotheresearchdocket.OurgoalforthispresentationistoprovideanoverviewofthegeneralnatureandpotentialscopeoftheseresearchactivitiesandtheunderlyingmotivationtoincludeeconomicresearchtoOWRI'soverallcommitmenttotheOregonwineindustry.
Asafirststep,wewillprovideanoverviewoftheresearchapproachesandtypicalscopeofresearchquestionsaddressedbyappliedeconomists.Ourgoalistodevelopasharedunderstandingofthepotentialcontributionsofthisnewresearchprogram.Economicresearchcanbeanexcellentcomplementtothelong‐standingeffortsofOWRIresearchandoutreachfaculty.Thisworkcanalsobedesignedtobuildupandextendmarket‐orientedresearchthathistoricallyhasbeenprovidedbytheOregonWineBoard.
Wewillconcludeourpresentationwithhighlightsofresearchfindingstodatefromourstudyofwineclubbestpracticesandclubmemberpreferences.Aswewilldetail,webeganthistwo‐phasedprojectbycontactingover500wineriesinOR,askingfortheircooperationindistributinganinternet‐basedquestionnairetotheirwineclubmembers.Wereceived75responsesfromORwineriesandasummaryoftheirresponseswillbepresentedalongwithdetailsaboutthesecondphaseoftheproject‐asurveyofwineclubmembers.
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BenefitsofEarlyAdoptionofPreventativePruningPracticesinManagingGrapevineTrunkDiseases
JonathanD.Kaplan1*,RenaudTravadon2,MonicaCooper3,VickenHillis4,MarkLubell5,KendraBaumgartner6
1Professor,DepartmentofEconomics,SacramentoStateUniversity,SacramentoCA;2AssistantProjectScientist,DepartmentofPlantPathology,UniversityofCalifornia,DavisCA;3ViticultureFarmAdvisor,UniversityofCaliforniaCooperativeExtension,NapaCA;4AssistantProfessor,DepartmentofHumanEnvironmentSystems,BoiseStateUniversity,BoiseID;5Professor,DepartmentofEnvironmentalScienceandPolicy,UniversityofCalifornia,DavisCA;6PlantPathologist,USDAARS,DepartmentofPlantPathology,UniversityofCalifornia,DavisCA
*CorrespondingAuthor:[email protected],916‐278‐4367
Despitethehighlikelihoodofinfectionandsubstantialyieldlossesfromtrunkdiseases,manywinegrapegrowerswaittoadoptfield‐tested,preventativepractices(delayedpruning,doublepruning,andapplicationofpruning‐woundprotectants)untilafterdiseasesymptomsappearinthevineyardataround10yearsold.WeevaluatenetbenefitsfromadoptionofthesepracticesbeforesymptomsappearinyoungCabernetSauvignonvineyardsandaftertheybecomeapparentinmaturevineyardstoidentifyeconomichurdlestoearlyadoption.WesimulateproductioninfiveregionsofCaliforniaandfindwidespreadbenefitsfromearlyadoption,increasingvineyardprofitablelifespans,insomecases,bymorethan50%.However,hurdlestoadoptionmayresultfromuncertaintyaboutthecostandreturnsfromadoption,laborconstraints,longtimelagsinbenefitsfromearlyadoption,growers’perceivedprobabilitiesofinfection,andtheirdiscountrate.Toovercomethesehurdleswerecommendthedevelopmentofextensionresourcestocommunicatethesebenefitsandpotentialhurdlestogrowerstoreduceuncertainty,leadingtoincreasedearlyadoption,resultingingreatervineyardprofitabilityandlifespans.Inthatrespect,aweb‐basedresourcewedevelopedisintroducedthatcommunicatesthebenefitsfrom,andpotentialhurdlestoearlyadoption.
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ProtectingYourCashFlowUsingWholeFarmRevenueProtection(WFRP)andOtherFarmFinancialManagementTools
KentFleming1,BeauOlen2*
1AdjunctProfessorandExtensionEconomist;2FacultyResearchAssistant,DepartmentofAppliedEconomics,OregonStateUniversity,CorvallisOR
*CorrespondingAuthor:[email protected],541‐737‐1321
EducatinggrowersaboutWholeFarmRevenueProtection(WFRP)andotherfarmfinancialmanagementtoolscanhelpthemmakemoreinformeddecisionstoprotecttheircashflow,preventfarmfailure,andencouragegrowthoftheOregonwineindustry.OurposterincludesanexampleofhowWFRPcanprotectcashflow,analysisofhowothergrowershaveusedWFRP,andanevaluationofthereturnoninvestment(ROI)fromwinegrapeinsurance.WefindthatthelowinsuranceparticipationratesinOregonarenotjustifiedbytheROIandthatmanyoftheearliestadoptersofWFRPwereinthePacificNorthwest.
Cashflowisafarm’sannualcashincome(inflow)minusitscashoperatingexpenses(outflow)fortheproductionyear.Cashflowisthemostimportantfinancialindicatortomonitorbecauseitistheprimarycomponentofworkingcapital,whichisallcashavailabletothefarminashorttime.Afarmcanhavealossinagivenyear,butitneedspositiveworkingcapitaleveryyeartocontinueoperating.Thus,protectingcashflowshouldbegrowers’highestfinancialmanagementpriority.
Onewaytopreventnegativecashflowistoforwardcontractsalesthatlockinaguaranteedpricebeforeharvest.ThiscanbeusedinconjunctionwithUSDAcropyieldinsurancethatguaranteesacertainlevelofproduction.AnotherwaytopreventnegativecashflowisWFRP,whichisaUSDAcropinsuranceprogramthatguaranteesupto85%ofwholefarmrevenue.WFRPisavailabletoanyfarmwithupto$8.5millioninrevenueanditprovidesadditionalsubsidiesforgrowingmorethanonecommodity,suchasredandwhitewinegrapes.WFRPisthefirstrevenueinsuranceproductavailableforwinegrapes.
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Notes:
Oregon Wine Research Institute 4017 Ag and Life Sci Building
Corvallis, OR 97331
www.owri.oregonstate.edu