1. Super-resolution Imaging by Ground State Depletion (GSD)

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1. Super-resolution Imaging by Ground State Depletion (GSD) Analysis of the spatial distribution of Syntaxin by STED within the basal plasma membrane of PC12 cells. STED microscopy allowed the investigation of cluster density and the determination of average Cluster sizes of 50 – 60 nm. [Science, Sieber JJ., 2007] Technology Seminar Speaker: Dr Christian May Product Manager, Wide-Field Technologies, Leica Microsystems Ltd All are Welcome! 2. Stimulated Emission Depletion (STED) Speaker: Dr Jochen Sieber Product Manager Super-resolution Technologies, Leica Microsystems Ltd 10 January 2012 4:30p.m. – 6:00p.m. Anatomy Seminar Room, L1-19, 1/F, Laboratory Block, 21 Sassoon Road, Faculty of Medicine Building, The University of Hong Kong. Ptk2-cells. NPC-staining: anti-NUP153/Alexa Fluor® 532 Microtubule-staining: anti--tubulin/Alexa Fluor® 647 Courtesy: Wernher Fouquet, Leica Microsystems In collaboration with Anna Szymborska and Jan Ellenberg, EMBL, Heidelberg, Germany. Co-organized by: Faculty Core Facility - The University of Hong Kong Leica Microsystems Ltd

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Technology Seminar. 1. Super-resolution Imaging by Ground State Depletion (GSD). Speaker: Dr Christian May Product Manager, Wide-Field Technologies, Leica Microsystems Ltd. 2. Stimulated Emission Depletion (STED). Speaker: Dr Jochen Sieber - PowerPoint PPT Presentation

Transcript of 1. Super-resolution Imaging by Ground State Depletion (GSD)

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1. Super-resolution Imaging by Ground State Depletion (GSD)

Analysis of the spatial distribution ofSyntaxin by STED within the basal plasma membrane of PC12 cells. STED microscopy allowed the investigation of cluster densityand the determination of averageCluster sizes of 50 – 60 nm.[Science, Sieber JJ., 2007]

Technology Seminar

Speaker: Dr Christian May Product Manager, Wide-Field Technologies, Leica Microsystems Ltd

All are Welcome!

2. Stimulated Emission Depletion (STED)

Speaker: Dr Jochen Sieber Product Manager Super-resolution Technologies, Leica Microsystems Ltd

10 January 20124:30p.m. – 6:00p.m.

Anatomy Seminar Room, L1-19, 1/F, Laboratory Block, 21 Sassoon Road, Faculty of Medicine Building, The University of Hong Kong.

Ptk2-cells. NPC-staining:anti-NUP153/Alexa Fluor® 532Microtubule-staining:anti--tubulin/Alexa Fluor® 647Courtesy: Wernher Fouquet, Leica MicrosystemsIn collaboration with Anna Szymborska and Jan Ellenberg, EMBL, Heidelberg, Germany.

Co-organized by:Faculty Core Facility - The University of Hong Kong

Leica Microsystems Ltd