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Transcript of 1 Characteristics of microbes Viruses Not cells; contain either RNA or DNA in envelope; can only...
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Characteristics of microbes
• VirusesNot cells; contain either RNA or DNA in envelope; can only multiply in living cells.
Major groups of microorganisms
• BacteriaUnicellular; procaryotic; rigid cell wall; absorb nutrients. Archaebacteria; eubacteria
Archaebacteria• Non-peptidoglycan cell walls• Have unusual metabolic capabilities.• Live in extreme environments.• Methanogens, halophiles and thermoacidophiles.
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Characteristics of microbes
• FungiEither uni- or multicellular; eucaryotic; rigid cell walls; absorb nutrients.
Major groups of microorganisms 2
• ProtozoaSingle celled; eucaryotic; lack rigid cell wall; ingest food.
• AlgaeEither uni- or multicellular; eucaryotic; rigid cell walls; chlorophyll; absorb nutrients.
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Structure/function of eubacteria
Size, shape and arrangement
Diameter LengthAve.
0.5 - 1 mup to 500 m
1.1 - 1.5 m wide 2.0 - 6.0 m long (E. coli )
Spherical
Rods
Spiral
coccus (sing.) cocci (pl.) meaning “berries”.bacillus (sing.) bacillus (pl.) meaning “little staffs”.
spirullum (sing.) spirilla (pl.)
Pleomorphy variety of shapes in a species
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Structure/function of eubacteria
Size, shape and arrangement 2
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Structure/function of eubacteria
Size, shape and arrangement 3
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Structure/function of eubacteria
Ultrastructure
Cell wall
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Structure/function of eubacteria
• Cell wall
Ultrastructure 2
Rigid, strong structure; gives and maintains shape.10 - 40% of dry weight of the cell.Usually essential for cell division.A barrier to some substances.Not homogeneous structures; depending on species, layered with different substances which have different thicknesses.
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Structure/function of eubacteria
• Peptidoglycan (a.k.a. murein)
Ultrastructure 3
Comprises N-acetylglucosamine, N-acetylmuramic acid and tetrapeptides.Peptidoglycan chains are cross-linked at the tetrapeptide units.Autolysin opens the network for addition of new polymer.
Shape determining part of the wall.Insoluble, porous polymer of great strength and rigidity.
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Structure/function of eubacteria Ultrastructure 3
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Structure/function of eubacteria
There are 2 types of cells wall in eubacteria
Ultrastructure 4
• Gram stain differently
• Differences in the layering and presence of outer membrane (in G -)explains the difference in Gram reaction and pathogencity.
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Structure/function of eubacteria
The G(+) cell wall
Ultrastructure 5
• Peptidoglycan content 50%cf. G(-) 10%
walls of G(+) bacteria appear thicker.• Contains techoic acid (may aid in the transport of cations).
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Structure/function of eubacteria
The G(-) cell wall
Ultrastructure 5
• More complex; has outer membrane covering a thin layer of peptidoglycan.
• Outer membrane: Contains lipolysaccharides (lipid portion causes toxic effects in infected animals).
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Structure/function of eubacteria
Cytoplasmic membraneUltrastructure 5
• Composition: Phospholipids 20 - 30% Proteins 50 - 70%
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Structure/function of eubacteria
Cytoplasmic membrane 2
Ultrastructure 6
• A site of specific enzyme activity, transport of molecules in and out of the cell, and invaginations participate in cell metabolism and replication.
• Some processes essential to the cell are located here.A barrier to most water-soluble molecules, and is more selective than the cell wall.
Permeases transport small molecules into the cell.
Also contains various enzymes for energy production and synthesis of cell wall.
• Photosynthetic bacteria or those with metabolisms based on gas exchange have elaborate intracellular extensions of the cytoplasmic membrane.This infolding increases the area for light pigments or gas exchange.
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Structure/function of eubacteria
Ultrastructure Cytoplasmic area
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Structure/function of eubacteria
Cytoplasmic area
Ultrastructure 6
• 80% water with nucleic acids, proteins, carbohydrates, lipids, inorganic ions, many low-molecular weight compounds and particles with various functions.
• Ribosomes. Free in cytoplasm, not membrane-bound.
• Cell inclusions (insoluble chemical substances accumulated) e.g. metachromatic granules (a.k.a volutin; a reserve of inorganic phosphate).
Lipids e.g. poly--hydroxybutyrate; reserve carbon and energy source.Sulfur: H2S-oxidizing bacteria.
Polysaccharides e.g. glycogen and starch; reserve energy sources.
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Structure/function of eubacteria
Ultrastructure
Nuclear area
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Structure/function of eubacteria
Nuclear area
Ultrastructure 6
• No nuclear membrane. Nuclear material found near the center of the cell and is attached to the mesosome-cytoplasmic membrane.
• Total nuclear material = nucleoid; consist of a single circular chromosome.
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Structure/function of eubacteria
Ultrastructure
Flagella
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Flagella(e)
Ultrastructure 6
• Thin, helical, hair-like filaments; extends from the cytoplasm through the cell wall. Observable in stained specimens.
• Propels the cell. Up to 100 m min-1. 3000 cell lengths min-1 cf. the cheetah’s top speed of 1500 body lengths min-1.
• Peritrichous flagellation
• Polar flagellation: monotrichous; lophotrichous; amphitrichous.
Structure/function of eubacteria
E. Coli © Dennis Kunkel
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Fimbria(e)
Ultrastructure 7
• Hair-like appendages not related to motility. Shorter and straighter than flagella
• Either polar or even distribution.Number from few to thousands.
• Enables adhesion. A factor in pathogenicity.
Structure/function of eubacteria
E. Coli © Dennis Kunkel
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Pilus(i)
Ultrastructure 8
• Usually longer than fimbriae.
• Number only one or two.
• Join cells in preparation for transfer of DNA.Thus also known as sex pili.
Structure/function of eubacteria
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Structure/function of eubacteria
Glycocalyx
Ultrastructure 9
• Layer of viscous material (usually polysaccharides) outside the cell wall. Observation: Indian ink stained specimens.
• When well defined: capsule.disorganized: slime layer.
• Function: Adhesion; protection against desiccation; prevent attachment by bacteriophages or attack by white blood cells.
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Characteristics of viruses
Living organisms?• Inert outside a living host• Obligatory intracellular parasites• Either: complex aggregation of non-living chemicals or simple living
microorganism.
Distinctive features• Single type of nucleic acid; DNA or RNA.• Protein coat surrounds the nucleic acid (NA).• Synthetic processes of a host living cell used to multiply.• Specialized structures for transfer viral NA to other cells.
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Characteristics of viruses
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Viral structure
Virion
A complete, fully developed viral particle composed of nucleic acid surrounded by a protective coat which also serves as a vehicle of transmission from one host cell to another.
Nucleic acid
• Contrasts with pro- and eucaryotic cells where DNA is the primary genetic material.
• Total NA: From a few thousand to as many as 250,000 nucleotide pairs.E. coli has 4 million nucleotide pairs.
• NA is single or double stranded; linear or circular.
• May be in separate segments e.g. influenza virus.
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Viral structure
Capsid
• Protein coat surrounding the nucleic acid.
• Capsomere: Protein subunits of the capsid.Arrangement is characteristic of each particular
virus. • Nucleocapsid: Capsid and nucleic acid as a unit.
• Capsids give virus types their typical shapes.
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Viral structure
Envelope
• Outer membranous layer; lipids, carbohydrates and proteins.
• Present in many animal and some plant viruses.
• In animal viruses: Host cell nuclear or plasma membranes contribute envelope lipids and carbohydrates.Envelope proteins are made by the virus.
• Some have spikes (a.k.a. peplomers); are carbohydrate-protein complexes.Thought to be involved in attachment to hosts.Useful in identification since they differ amongst viruses.
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Viral structure
Four general morphological types of virion structure
• Helical virusesCapsomeres arranged in a helical around the nucleic acid.
• Polyhedral virusesCapsids are icosahedral in arrangement (20 equilateral triangular faces and 12 corners).
• Enveloped viruses Roughly spherical but pleomorphic as the envelope is not rigid. Capsid can be helical or polyhedral. Non-enveloped viruses are “naked” viruses.
• Complex viruses Have capsid symmetry but neither purely helical or polyhedral.
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Viral structure
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The eucaryotic cell
Protozoa, algae and fungi are eucaryotic.
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The eucaryotic cell
Ultrastructure
• Flagella and ciliaFew and long - FlagellaShort and few - ciliaMotility; movement of substances (cilia)Both: 9 ring pairs and 2 central microtubules.
• GlycocalyxSurrounds animal cells.Strengthens cells; also means of attachment to other cell.
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The eucaryotic cell
Ultrastructure 2
• Cell wallAlgae and some fungi: cellulose.Fungi: chitin.
• Cytoplasmic membraneLike procaryotes: Phospholipid bilayer containing proteins.Contains CHO attached to proteins and sterols not found in procaryotes.Capable of endocytosis.
• CytoplasmResemble that of procaryotes.Has cytoskeleton and exhibits cytoplasmic streaming.Unlike procaryotes, many important enzymes are sequestered in organelles.
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The eucaryotic cell
Ultrastructure 3
• Cell wallAlgae and some fungi: cellulose.Fungi: chitin.
• Cytoplasmic membraneLike procaryotes: Phospholipid bilayer containing proteins.Contains CHO attached to proteins and sterols not found in procaryotes.Capable of endocytosis.
• CytoplasmResemble that of procaryotes.Has cytoskeleton and exhibits cytoplasmic streaming.Unlike procaryotes, many important enzymes are sequestered in organelles.
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The eucaryotic cell
Ultrastructure 4
Organelles
• NucleusContains DNA in the form of chromosomes. Most characteristic of eucaryotes.
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The eucaryotic cell
Ultrastructure 5
Organelles
• Endoplasmic reticulumNot present in procaryotes.Provides surface for chemical reactions.Serves as transport network.Stores synthesized molecules.
• RibosomesAttached to outer surface of ER but also found free.Larger than procaryotic ribosomes.Site of protein synthesis.
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The eucaryotic cell
Ultrastructure 6
Organelles
• Golgi complexConsists of cisternae.Secretion; CHO and glycoprotein synthesis.
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The eucaryotic cell
Ultrastructure 7
Organelles
• Mitochondrion(a)Folding of inner membrane provides large surface area for chemical reactions e.g. ATP production (primary site) and cellular respiration.Can multiply on their own by fission.
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The eucaryotic cell
Ultrastructure 8
Organelles
• LysosomesFormed from golgi complexes.Contain digestive enzymes to breakdown molecules and invasive bacteria.
• CentriolesRole in cell division and as basal bodies in the formation of flagella and cilia.
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The eucaryotic cell
Ultrastructure 6
Organelles
• ChloroplastAlgae/green plants only.Contains DNA, ribosomes, chlorophyll and photosynthetic pigments.Can multiply on their own by fission.