Vecto

r con

trol

MUC20

GAPDH

MUC20130

55

36

250

(kDa)

HEK293

Aanti-MUC20 antibody IgG control

B

Supplementary Fig. S1. Specificity of anti-MUC20 antibody. (A) Western blotting showed that anti-MUC20 antibody specifically recognizes MUC20 overexpressed in HEK293 cells. The arrow indicates MUC20 protein. GAPDH is an internal control. (B) Immunohistochemistry of MUC20 in human kidney. The anti-MUC20 antibody strongly stained proximal, but not distal, convoluted tubules, which is consistent with the previous report (J Biol Chem. 2004;279:1968-79). The star (*) indicates the distal convoluted tubule. IgG control did not show specific binding.

*

50 μm