Vector control
1
Vector control MUC20 GAPDH MUC20 130 55 36 250 (kDa ) HEK293 A anti-MUC20 antibody IgG control B Supplementary Fig. S1. Specificity of anti-MUC20 antibody. (A) Western blotting showed that anti-MUC20 antibody specifically recognizes MUC20 overexpressed in HEK293 cells. The arrow indicates MUC20 protein. GAPDH is an internal control. (B) Immunohistochemistry of MUC20 in human kidney. The anti-MUC20 antibody strongly stained proximal, but not distal, convoluted tubules, which is consistent with the previous report (J Biol Chem. 2004;279:1968-79). The star (*) indicates the distal convoluted tubule. IgG control did not show specific binding. * 50 μm
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A. B. Vector control. MUC20. a nti-MUC20 antibody. IgG control. ( kDa ). 250. MUC20. 130. *. 55. 36. 50 μ m. GAPDH. HEK293. - PowerPoint PPT Presentation
Transcript of Vector control
Vecto
r con
trol
MUC20
GAPDH
MUC20130
55
36
250
(kDa)
HEK293
Aanti-MUC20 antibody IgG control
B
Supplementary Fig. S1. Specificity of anti-MUC20 antibody. (A) Western blotting showed that anti-MUC20 antibody specifically recognizes MUC20 overexpressed in HEK293 cells. The arrow indicates MUC20 protein. GAPDH is an internal control. (B) Immunohistochemistry of MUC20 in human kidney. The anti-MUC20 antibody strongly stained proximal, but not distal, convoluted tubules, which is consistent with the previous report (J Biol Chem. 2004;279:1968-79). The star (*) indicates the distal convoluted tubule. IgG control did not show specific binding.
*
50 μm
