The analytical laboratorySchool of Biological Sciences

Dr Bowater Prof. RichardsonInteractions of proteins with DNA The bacterial nitrogen cycle & anaerobic metabolism of bacterial pathogens

Dr Brearley Dr WatmoughPlant metabolic biochemistry Enzymology of Nitric oxide generation/metabolism Oxidases and peroxidases in aerobic metabolism Dr ClarkeBiophysical/spectroscopic analysis of protein-protein interactions

Dr HemmingsX-ray structural and mechanistic studies of enzymes

Catalytic properties of metalloproteins involved in bacterial respiration which enable bacteria

to exploit host cell environments in symbiosis and pathogenesis

The analytical laboratorySchool of Biological Sciences

Facilities & expertise

Protein expression.

Protein purification

Protein characterization

Chromatographic analysis/purification of small molecules

The analytical laboratorySchool of Biological Sciences

Protein purification

Under aerobic and anaerobic conditionsfrom Category 2 microorganismsLicense/authority from Defra, HSE

Protein purification on analytical, pilot and larger scales

Protein structure determinationX-ray crystallography with access to beam time on synchrotonsRobotic crystallization trials

Protein characterization - capabilities

Structure-informed protein characterization and rationale design is available

Protein structure prediction and modellingEnzymology, especially of metalloproteinsVariety of spectroscopic techniquesTime-resolved spectroscopy of metalloproteinsFast reaction kinetics

Analytical ultra centrifugationHydrodynamic properties of proteins

Protein characterization - capabilities

In absence of crystal structures, protein structures are modelled

Isothermal calorimetryto study protein-ligand interactions

True thermodynamic parameters for any reaction are obtained by ITC

0.0 0.5 1.0 1.5 2.0 2.5

-10

-8

-6

-4

-2

0

0 10 20

-0.4

-0.2

0.0

0.3 mg/ml (C12A)Mur + 1 mMMn(II)Time (min)

µca

l/sec

Molar Ratio

kcal

/mol

e of

inje

ctan

t

Metabolic and small molecule analysis

Gas Chromatography with liquid and headspace samplingElectron Impact GC-Mass Spectrometry Flame Ionization Detection Electron Capture Detection

Fermentation products, gases, natural oils are analyzed by GC

Metabolic and small molecule analysis

Tracer analysisStable isotopes and radioisotopesLicensed by Env. Agency

High Pressure Liquid Chromatograhy

on analytical and semi-prep scale

UV, DAD, suppressed ion conductivity, fluorescence &

radiochemical detection

HPLC-Mass Spectrometry

HPLC method development is available

Contact details & sponsors

Contacts

c.brearley@uea.ac.uk

n.watmough@uea.ac.uk