Standard Data Analysis -Calibration

ChemStation – Level 1 - Training

1. Inject standards

2. Set-up appropriate integration events for the low standard.

3. Begin a new calibration table with level one, the lowestlevel. Fill in the dialog.

4. Integrate each additional standard level and add to the calibration table.

5. Examine the calibration curve and save it to the method.

Building a Calibration Table

Example Calibration

1. Standard 30.0 ISTD 50.02. Standard 50.0 ISTD 50.03. Standard 75.0 ISTD 50.0

ng/L ng/L

3 Levelsmin1 2 3 4 5 6

mAU

0

20

40

60

80

100

DAD1 A, Sig=254,4 Ref=550,100 (DEMO\005-0101.D)

0.7

47

1.0

21

2.5

65

5.8

37

Standard ISTD

Define signals to be evaluated during a method.

Signal Details

Calibration Settings

Load the Signal(s) for the Low Level Standard and Integrate

Create a Calibration Table: Level One

Fill in:Compound NameGroupAmount

Check if:Reference PeakISTDIndicate #

Calibration Table Overview: Fill in the Dialog

Load the Signal - Add Level Two

Add Additional Levels

Compound, Amt, Area, Rsp Factor,Ref, ISTD#

Compound, Grp, Amt, Low LimitHigh Limit

Compound, Amt, Area, Def, CurveType, Origin

Compound, Area, Rsp%, Def, +-, Pk Usage

Calibration Table Options

Use Low and High Limits as qualifiers to confirm peak

identification.

Compound Details

Check Def to apply thedefault curve type and origin from the Calibration Settingsdialog box.

Curve TypesPiece wise ExponentLinear QuadraticLog CubicPower Average Rsp/Amt

OriginIgnoreIncludeForceConnect straight segment

Peak Details

Define the columns included in your Calibration Table

Edit Calibration Table Options

Calibration TaskTool New Calibration

Table

Recalibrate withCurrent Chromatogram

Add New Level Signal Details

Add New Peaks

Select Calibrated peak(s) fromChromatogram and delete fromcalibration table.

Select peak(s) from chromatogram and addto calibration table

Select compoundsfrom chromatogram and recalibrate

Edit CalibrationTable Options

Quantification Tools

Save Table to Method

To Save the Calibration Table as Part of a Method...

Signal Options

Customized display on screen and in reports

Annotations

Use it for a customized chromatogram in slides or publications

Easy placing and moving of annotations

Selectable fonts and colors

The Annotation sidebar can be activated within all toolsets

Signal Manipulations

Overlay, subtract and align signals

Lab Time !!!! Lab 7 - Calibration