Post on 18-Feb-2021
Practical PhD Course in Mouse Transgenesis
Aarhus University October 26 to November 6 2015
Objectives: To introduce students to the principle techniques used to modify the murine genome such as transgenesis, knock out, knock in, TALEN- and CRISPR mutagenesis. The course includes hands on exercises and demonstrations of the following techniques: - Isolation of different stages of pre-implantation embryos - Culture of pre-implantation embryos - Pro-nuclear injection - Blastocyst injection - Preparation of embryonic fibroblasts - Derivation of ES cells from blastocysts - Culture, transfection and selection of embryonic stem cells - Isolation and analysis of single ES cell colonies - Cryopreservation of murine embryos and sperm The students will have the possibility to discuss their own projects with respect to the relevant techniques.
Duration: 10 days; ECTS points: 6.5; Number of participants: 4-‐6 Fee: 5.000 DKK for external students, please inquire if this is prohibiting
The fee does not cover travel and accommodation Organizer: Ernst-‐Martin Füchtbauer, Dept. Molecular Biology and Genetics Location: University of Aarhus; Date: October 26 to November 6, 2015
Application deadline: August 28, 2015 Sent application with a short letter of motivation to: emf@mbg.au.dk