Practical PhD Course in Mouse Transgenesis

Aarhus University October 26 to November 6 2015  

 

Objectives: To introduce students to the principle techniques used to modify the murine genome such as transgenesis, knock out, knock in, TALEN- and CRISPR mutagenesis. The course includes hands on exercises and demonstrations of the following techniques: - Isolation of different stages of pre-implantation embryos - Culture of pre-implantation embryos - Pro-nuclear injection - Blastocyst injection - Preparation of embryonic fibroblasts - Derivation of ES cells from blastocysts - Culture, transfection and selection of embryonic stem cells - Isolation and analysis of single ES cell colonies - Cryopreservation of murine embryos and sperm The students will have the possibility to discuss their own projects with respect to the relevant techniques.

Duration:  10  days;  ECTS  points:  6.5;  Number  of  participants:  4-‐6  Fee:  5.000  DKK  for  external  students,  please  inquire  if  this  is  prohibiting  

The  fee  does  not  cover  travel  and  accommodation  Organizer:  Ernst-‐Martin  Füchtbauer,  Dept.  Molecular  Biology  and  Genetics  Location:  University  of  Aarhus;  Date:  October  26  to  November  6,  2015  

Application  deadline:  August  28,  2015 Sent  application  with  a  short  letter  of  motivation  to:  emf@mbg.au.dk