Nikon Suggested Imaging Configuration

NIKON “NIS-ELEMENTS” TRAINING NEW PRODUCTS MEETING 2006February 7,2006

SingaporeNikon Instech Co.,Ltd.

Sachiko Morimoto

Purpose of Current Biological Research Market

living cell observation → imaging → presentation & analysis

• Microscope :motorization, high quality image etc.• CCD Camera: high resolution, high speed etc.• Software: easy to use, multiple functions, continues

improvement (up-grade)

3D imaging

Timelapse(Time series observation)

Microscope

Main bodyEyepiecesNosepiece

StageObjective lens

Condenser

DSC

NIS Elem

entsNikon’s approach for System Solution

Periphery products (Nikon or 3rd party)

Application

1. Microscope hardware(~2003)2. Start Imaging equipments & software(2004~2005)3.Emphasize on System products for each application (2006~)

and more ・・・

Overview of Nikon System Solution

• Microscope: TE2000 series, 90i• Camera:DS5M,DS5Mc,DS2MBWc,DS2M,DS2Mc,

DXM1200F, DXM1200c• Software :NIS Elements AR/BR + plug in

Nikon System Solution

Some third party’s accessories if necessary.

(ex. Motorized shutter, motorized stage, incubator

system 　 etc.)

Merit of System offer sales

• If we can offer whole system to your customers, it can give more reliability to customers.

• Customer can receive full support from Nikon.

• It is easy for customers to choose a system from one manufacture.

Nikon’s suggested configuration ~ Multi observation from 3D to 6D X,Y,Z,T,λ,Multipoint ~

X

YZ

Multi point

TimeTime

For living cell and observing the changes in time series.

For multiple stained sample

For thick sample and 3D imaging

Especially for well plate users

Application• Fluorescence • DIC• Multi Fluorescence • Multi Fluorescence ＋ DIC• Fluorescence Timelapse （ with PFS)• DIC Timelapse （ with PFS)• Fluorescence + DIC Timelapse （ with PFS)• Multi Fluorescence + DICTimelapse （ with PFS)• Multipoint• EDF• Fluorescence Deconvolution• Real time Deconvolution

Third party accessories

Sutter : EX/BA filter wheel Lambda 10 Prior: Motorized stage Pro

Scan H117E2

UNIBLITZ: Shutter for Epi/Dia

Third party accessories

CCD Camera : Roper Scientific

Nikon’s Current System

Motorized stage

PFS （ AF ） Motorized shutter

CO2 chamber controller

Light sourceEX filterwheelCCD Camera

DIA Motorized shutter

　　　　　　　　　　 CO2chamber(TOKAIHIT)

BA filterwheel

PC （ NIS-element

Objective lens

Nikon Exclusive Objective lens Temperature correction *World First!*World First!

Aberration is corrected in temperature 23-37degrees　 　･･･ Friendly for living cell

CFI Plan Apo TIRF60xoil N.A.1.49, W.D.0.13CFI Plan Apo TIRF100xoil N.A.1.49, W.D. 0.12

Nikon Exclusive Perfect Focus System Optical offset function maintain the focus position of

your interested plane by ‘m sec’‘m sec’ order. Stable focusing for time-lapse imaging For 2 to 3 days or longer time lapse imaging. Using IR LED for the light source

TE2000E with New TIRF obj. and NIS Elements are the best TE2000E with New TIRF obj. and NIS Elements are the best choice for living cell time lapse system!choice for living cell time lapse system!

Market Demands

More & More digital imaging

High Quality Imaging

PC control for various applications

Let’s work together as Total Solution Provider!

Thank you !