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Miniaturising Pressure-Driven-Flow

:Translating the laboratory set-up into a commercial instrument’

John Watson

Business Development Manager, TTP Ventus.

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Microfluidic pump optionsPeristaltic pump Syringe pump Pressure driven flow

Flow stability and precision Low Medium Excellent

Response time Low Low Excellent

Ultra smooth flow Low Medium Excellent

Direct handling of media (cross contamination)

Yes Yes No – reduces risk of cross contamination

Size, weight, noise Medium Poor Poor

Gas injection No No Yes

Injection of Nano-volume sample

Poor Good Medium*

Sample agitation Possible Not possible Possible

Sample temperature control

Possible Not Possible Possible

Dynamic flow range Good Poor Excellent

Fluid recirculation Yes No Yes**

* Limited only by flow sensor ** Valves needed

Pressure driven flowFlow stability and precision Excellent

Response time Excellent

Ultra smooth flow Excellent

Direct handling of media (cross contamination)

No – reduces risk of cross contamination

Size, weight, noise Poor

Gas injection Yes

Injection of Nano-volume sample

Medium*

Sample agitation Possible

Sample temperature control

Possible

Dynamic flow range Excellent

Fluid recirculation Yes**

*** Live poll active ***

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Why miniaturise Pressure-Driven-Flow ? Microfluidic trends

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Diagnostics

Point-of-Care (POC)MiniaturisationRapid-readout

Microfluidics holds the key!

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Attributes of Pressure-Driven-Flow

* Limited only by flow sensor ** Valves needed

Pressure driven flow

Flow stability and precision Excellent

Response time Excellent

Ultra smooth flow Excellent

Direct handling of media (cross contamination)

No – reduces cross contamination

Size, weight, noise Poor

Gas injection Yes

Injection of Nano-volume sample Medium*

Sample agitation Possible

Sample temperature control Possible

Dynamic flow range Excellent

Fluid recirculation Yes**

Pressure driven flow

Flow stability and precision Excellent

Response time Excellent

Ultra smooth flow Excellent

Direct handling of media (cross contamination)

No - reduces risk of cross contamination

Size, weight, noise Great (with Disc Pump)Gas injection Yes

Injection of Nano-volume sample Medium*

Sample agitation Possible

Sample temperature control Possible

Dynamic flow range Excellent

Fluid recirculation Yes**

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Disc Pump – For next generation Pressure-Driven-Flow systemsHigh precision flow control

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Disc Pump – For next generation Pressure-Driven-Flow systems

Response speed

Rapid response and precise flow-rate control, enabled by Disc Pump.

Flow rate stability

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Disc Pump – For next generation Pressure-Driven-Flow systems

Ultra-smooth flow

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Disc Pump – For next generation Pressure-Driven-Flow systems

Cost Silent operation

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Enabling innovation – Droplet microfluidics

Miniaturisation

Faster processing

Improved STATS

Lower cost

Control & precision

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Disc Pump – Organ-on-a-chip

Reinventing cellular research

TTP Cellpod

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• TTP Ventus evaluation kit and integration of SLF3x flow sensors

• Closed loop flow control

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TTP Ventus Disc Pump – New HP Series

• Pressure > 800 mbar

• Vacuum > 500 mbar

*** Poll now active ***

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• Pressure-Driven-Flow (PDF) offers many advantages with microfluidic systems

• The TTP Ventus Disc Pump enables miniaturisation and innovation in PDF systems with:Accurate & precise flow controlRapid response speedUltra smooth flow Silent operationCost effective solution

To summarise:

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TTP Ventus' pump will be the backbone of our point-of-care device. It is a powerful and compact design, and enables precise control of our microfluidics. The performance of the pump is only surpassed by the quality of the team behind it.

Victor Serdio, CTO, Mursla Ltd.

The TTP Ventus pump was used to develop innovative reagent delivery ideas for microfluidic point-of-care diagnostic devices. The pump’s precision and ultra-controllability offers bubble-free and pulse-free reagent delivery into microfluidic devices. Additionally, the size and customizability of these pumps makes them the best solution for fluid handling in portable infectious disease diagnostic devices. Prof. W Balachandran (Bala), Research Professor,

Brunel University London.

Thanks for listening!

Any questions?John.watson@ttpventus.com