Post on 03-Feb-2016
description
Normal Enzyme Activity
Cuvette 1 2Reaction Mixture (uL) 900 900Normal Serum (uL) 100 75Time (sec) Absorbance Cuv.1 Absorbance Cuv.2
0 0.3 0.215 0.355 0.24530 0.408 0.28845 0.458 0.32360 0.506 0.35775 0.553 0.39490 0.608 0.428
105 0.652 0.461120 0.695 0.497
Absorbance/Min (30-90) 0.2 0.14
dA=E.dC.l E= 6.2dC (mmol/mL/min) 0.032258064516129 0.02258064516129Activity U (umol/min) 0.32258064516129 0.225806451612903
20 30 40 50 60 70 80 90 1000
0.1
0.2
0.3
0.4
0.5
0.6
0.7
Absorbance vs Time (Cuv.1)
Time
Abso
rban
ce
20 30 40 50 60 70 80 90 1000
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
Absorbance vs Time (Cuv.2)
Time
Abso
rban
ce
20 30 40 50 60 70 80 90 1000
0.1
0.2
0.3
0.4
0.5
0.6
0.7
Absorbance vs Time (Cuv.1)
Time
Abso
rban
ce
20 30 40 50 60 70 80 90 1000
0.05
0.1
0.15
0.2
0.25
0.3
0.35
0.4
0.45
Absorbance vs Time (Cuv.2)
Time
Abso
rban
ce