Kenneth DawsonCentre for BioNano Interactions

EMEA ITF Group on NanotechnologiesApril 29th 2009

Website: www.cbni.eu

BioNanoInteractionsArea(s) of expertise

Nanoparticle synthesis Protein Corona

Transcriptomics and Proteomics

Disease / therapy

In vivo effects

IntracellularVisualization

Dispersion and characterisation

GLP

Class III nanoparticle synthesis

Class III Protein ExpressionClass III Cell Culture

New Institute,Integrated NanoAnd Biology, Good Laboratory Practice (?)

160-200 people

Protein Corona paradigm

Nanoparticles utilise existing biological pathways (in new ways?)

Uptake connected to protein corona

Nanotechnology for the Pharmaceutical industry

1

2 6

34

5

K15=[5][1]

0

50

100

150

200

250

0 50 100 150 200 250

Exposure time, minutes

FITC

log

37C 4C NaN3

1

2

3

65

4 0

5

10

15

20

25

30

0 50 100 150 200 250 300

Exposure time, minutes

FITC

log

37C 4C NaN3

Completely different uptake behaviour

0 30 60 90 120 150 180 210 240

0

5

10

15

20

25

30

35

Cel

l Flu

ores

cenc

e In

tens

ity

Exposure time, minutes

37C 4C NaN3

Distinct localisation in ER

Clear energy dependence

Quality And Standards Issues

Sds-gel (4% stacking gel and 10% resolving gel)

particles

Free dye (<6 kda)

Much of the literature in this area questionable!

Also observed for FITC-dextrans etc., not only nanoparticles.

Commercial samples leak - free dye!

new methods in-situ characterization

NanoSight Spinning Disc Centrifugation

SiO2 nanoparticles with increasing plasma concentration

Rel

Wei

ght

NIST Gold nanoparticles 30nm and 60nm in citrate buffer