Kenneth Dawson Centre for BioNano Interactions€¦ · Kenneth Dawson Centre for BioNano...
Transcript of Kenneth Dawson Centre for BioNano Interactions€¦ · Kenneth Dawson Centre for BioNano...
Kenneth DawsonCentre for BioNano Interactions
EMEA ITF Group on NanotechnologiesApril 29th 2009
Website: www.cbni.eu
BioNanoInteractionsArea(s) of expertise
Nanoparticle synthesis Protein Corona
Transcriptomics and Proteomics
Disease / therapy
In vivo effects
IntracellularVisualization
Dispersion and characterisation
GLP
Class III nanoparticle synthesis
Class III Protein ExpressionClass III Cell Culture
New Institute,Integrated NanoAnd Biology, Good Laboratory Practice (?)
160-200 people
Protein Corona paradigm
Nanoparticles utilise existing biological pathways (in new ways?)
Uptake connected to protein corona
Nanotechnology for the Pharmaceutical industry
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K15=[5][1]
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Exposure time, minutes
FITC
log
37C 4C NaN3
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Exposure time, minutes
FITC
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37C 4C NaN3
Completely different uptake behaviour
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Cel
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Exposure time, minutes
37C 4C NaN3
Distinct localisation in ER
Clear energy dependence
Quality And Standards Issues
Sds-gel (4% stacking gel and 10% resolving gel)
particles
Free dye (<6 kda)
Much of the literature in this area questionable!
Also observed for FITC-dextrans etc., not only nanoparticles.
Commercial samples leak - free dye!
new methods in-situ characterization
NanoSight Spinning Disc Centrifugation
SiO2 nanoparticles with increasing plasma concentration
Rel
Wei
ght
NIST Gold nanoparticles 30nm and 60nm in citrate buffer