INFLUENZA INFLUENZA TRANSPORT MEDIUMTRANSPORT MEDIUM

MEDIA PREPARATION TEAMMEDIA PREPARATION TEAMMiguel Garza, SupervisorMiguel Garza, Supervisor

Martha Thompson, Viral Isolation LaboratoryMartha Thompson, Viral Isolation Laboratory

IngredientsIngredients

Tryptose Phosphate BrothTryptose Phosphate Broth GelatinGelatin Penicillin 250,000 units per mLPenicillin 250,000 units per mL Streptomycin Sulfate 0.25 grams per Streptomycin Sulfate 0.25 grams per

mLmL Distilled WaterDistilled Water

PreparationPreparation

Combine in a 4 liter flaskCombine in a 4 liter flask Dissolve by stirringDissolve by stirring Autoclave 30 minutes at 121° CAutoclave 30 minutes at 121° C Add aseptically Penicillin 250,000 Add aseptically Penicillin 250,000

units and Streptomycin Sulfate 0.25 units and Streptomycin Sulfate 0.25 grams grams

Dispense aseptically 3.0 mls to Dispense aseptically 3.0 mls to 1998(aprox.) sterile tubes1998(aprox.) sterile tubes

Label each tube: INFLUENZA TRANSPORT, Label each tube: INFLUENZA TRANSPORT, 93515, lot number, expiration and Texas 93515, lot number, expiration and Texas Dept. of State Health Services, Austin, TX Dept. of State Health Services, Austin, TX 7875678756

Expiration date is 12 months from date of Expiration date is 12 months from date of preparationpreparation

Store in Media Production Freezer at -25 Store in Media Production Freezer at -25 °C°C

Deliver 7 tubes to Virology Team for Deliver 7 tubes to Virology Team for quality controlquality control

QC of Transport MediaQC of Transport Media

SterilitySterility ToxicityToxicity Growth assayGrowth assay

Storage at -70° C for 72 hrsStorage at -70° C for 72 hrs Storage at 4° C for 24 hrsStorage at 4° C for 24 hrs Undilute, 1:5, 1:10Undilute, 1:5, 1:10

Parallel testing old/new lots Parallel testing old/new lots

Viral Transport MediaViral Transport Media Viral transport mediumViral transport medium Hanks balanced salt solutionHanks balanced salt solution Tryptose-phosphate broth Tryptose-phosphate broth Sucrose-phosphate brothSucrose-phosphate broth Cell culture mediumCell culture medium Veal infusion brothVeal infusion broth

Supplement with a protein or stabilizer, bovine Supplement with a protein or stabilizer, bovine serum albumin or gelatin at 0.5 to 1%.serum albumin or gelatin at 0.5 to 1%.

AntibioticsAntibiotics