STLCC_CPLS;Morrison 10/20/2014 Page 1

Prepared by: Bob Morrison

FVCC, Instrumentation Specialist

Mar 2010,, Latest Revision July 2014

Image and Video Capture

(Scope, Camcorder, Camera, Scanner) @ BRDG

Capture Scope Images

Black/White/Transparency/Gel

Scanning for Densitometer

Color or B/W Scanning using UMAX

and Magicscan app.

Scan using Umax Astra 3400

Camcorder/Camera

Canon HFM31 @BRDG

Capture Scope Video

Image Capture: Scan, UMAX Astra 3400 Scanner

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1. Select VistaScan

2. Select Beginner in new window for most uses

3. Select Advanced to change default settings

Image Capture: Scan Astra 3400 , Set Location for Scans

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1. Select Preferences to set location

for scanned files.

2. Select Scan Destination on popup

tab as shown

3. Select a Drive for location of

flashdisk or temporary location

reserved for these scans as shown

Image Capture: Scan Astra 3400 , Select Type of Image

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1. Select type of image file, Jpeg is

recommended , Tiff is default.

2. Select quality/size of output file,

Good = 1000 kb file size

Image Capture: Scan Astra 3400, Select Type of Document

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1. Select type of Scan to initiate actual

scanning process

2. When done, go to location of

scanned files to verify images.

Image Capture; Scope R124

• Scope Video/Image Capture

– Turn on Scope and Obtain desired view in eyepiece, then rotate View

Selector knob to Camera view (CCW)

– Ensure that small MTI Camera adapter box is “on” (Green light) and that

output line is connected to the Belkin USB to S-Video Conversion box

– Logon PC and follow remaining steps in this SOP

• Camera or other Devices

– Connect Camera output to front face port of MTI Camera adapter box and

make sure it is “on”

– Connect line from MTI output to input port on Belkin USB conversion box

– Connect Belkin output line to PC USB port

– Logon PC and follow remaining steps in this SOP

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Microscope: Axiovert 25 @ BRDG R124 / SLCC # 0097770

Light Source Adjust

Off/On

Focus &

Fine focus

Filter Guide

Phase, Clear, Varel

Light Source

Condenser Light Diaphragm (must be pulled

fully forward)

Occular/Eyepiece

Reflector Modules

Filters/Fluorescence

View Selector Knob

Eyepiece (clockwise)

Projection (ccw)

Aperture Center Screws

Diaphragm Pushrod

Condenser Light Shunt

HBO Aux Light Source

Camera

PC/Monitor

To Projector

MTI-DAGE Camera

Control Unit (CCU)

Off/On

W green

indicator

Set Gain to

“manual”

Beklin S-

Video Box

Image Capture

Scope R124:

Startup and

Preview (prepared by Patrick Karl, Campus IT

Support, June 2014)

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Turn on the computer, microscope, and camera and capture devices. Verify that the capture device’s USB

cable is plugged into one of the computer’s USB port.

Once you are signed into the computer and have the Windows Desktop open, find the icon labeled

“Microscope Image Capture” (AmCap) and open it.

Once MicroScope Image Capture (AmCap) has opened, you should be able to see the preview from the

microscope already showing in the window. If NOT, go to end of this doc for settings verification.

While previewing the live image, move the slide around to your desired location for your image capture.

Once you have the desired screen depicting the image you want to capture, you will need to use the

Microsoft Snipping Tool.

Image Capture Scope R124: Use Microsoft Snipping Tool (prepared by Patrick Karl, Campus IT Support, June 2014)

STLCC_CPLS;Morrison 10/20/2014 Page 9

Open the Microsoft Snipping Tool, the icon is on the desktop or you can find it under

Start/Programs/Accessories.

Image Capture

Scope R124:

Save Images (prepared by Patrick Karl, Campus IT

Support, June 2014)

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Once Snipping Tool opens, use the cross hairs and click and drag the desired area for image capture.

Release mouse button to capture your image.

With image captured within Snipping Tool, you can do a File Save As and save to your desired location.

There are limited photo editor tools (pen, highlighter, etc) inherent within the Snipping Tool software after

you capture your image. You may also choose the file type format at this time (IE-JPG, PNG, etc)

Image Capture Scope R124: Edit Saved Images in Photoshop (prepared by Patrick Karl, Campus IT Support, June 2014)

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Open Adobe Elements 4.0 or other photo editor. Adobe Elements is available on your desktop or

from the Start Menu.

Once Adobe Photoshop Elements program has started, and you receive the splash screen shown

below, select the option titled “Start From Scratch” on the top right.

Image Capture

Scope R124:

Edit in Photoshop,

Select Files (prepared by Patrick Karl, Campus IT

Support, June 2014)

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When Elements opens you will be presented with the following New dialog box windows shown

below. Click on Cancel to get to the main Photoshop Elements menu window.

From the Element menu bar, select File and Open. This will open a File Open dialog box where you

can browse to your file locations and select the image(s) you wish to open. You may select one or

more images at a time by holding down Control while selecting the files.

Image Capture Scope R124: Images open for Edit (prepared by Patrick Karl, Campus IT Support, June 2014)

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Your files will open up in Elements for your dissemination and or editing purposes.

Image Capture Scope R124: Troubleshooting Tips (prepared by Patrick Karl, Campus IT Support, June 2014)

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IF YOU DO NOT SEE AN IMAGE AFTER OPENING AMCAP (Microscope Image Capture):

Verify that all hardware has been turned on and is connected, including the capture device USB out

cable to one of the computer’s USB port

Verify that Preview is selected under Options in AmCap (Microscope Image Capture). If not, then click

it to turn it on.

If you still do not see an image then go to Options/Video Capture Filter and select the Custom

Properties Tab and select Video Input and verify or select Input method is set to #2) S-Video.

If you still do not see an image after this, go to Options/Video Capture Filter and select the Video

Decoder tab and in the Video Standard field verify or select NTSC_M_J.

If you still do not see an image after this, call #5113 to contact the Computer Support Center so that a

ticket can be opened for a technician to be dispatched to resolve the issue.

Video Capture; Scope R124

• Scope Video/Image Capture

– Turn on Scope and Obtain desired view in eyepiece, then rotate View

Selector knob to Camera view (CCW)

– Ensure that small MTI Camera adapter box is “on” (Green light) and that

output line is connected to the Belkin USB to S-Video Conversion box

– Logon PC and follow remaining steps in this SOP

• Camera or other Devices

– Connect Camera output to front face port of MTI Camera adapter box and

make sure it is “on”

– Connect line from MTI output to input port on Belkin USB conversion box

– Connect Belkin output line to PC USB port

– Logon PC and follow remaining steps in this SOP

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Microscope: Axiovert 25 @ BRDG R124 / SLCC # 0097770

Light Source Adjust

Off/On

Focus &

Fine focus

Filter Guide

Phase, Clear, Varel

Light Source

Condenser Light Diaphragm (must be pulled

fully forward)

Occular/Eyepiece

Reflector Modules

Filters/Fluorescence

View Selector Knob

Eyepiece (clockwise)

Projection (ccw)

Aperture Center Screws

Diaphragm Pushrod

Condenser Light Shunt

HBO Aux Light Source

Camera

PC/Monitor

To Projector

MTI-DAGE Camera

Control Unit (CCU)

Off/On

W green

indicator

Set Gain to

“manual”

Beklin S-

Video Box

Video Capture

Scope R124:

Startup and

Preview (prepared by Patrick Karl, Campus IT

Support, June 2014)

STLCC_CPLS;Morrison 10/20/2014 Page 17

Turn on the computer, microscope, and camera and capture devices. Verify that the capture device’s USB

cable is plugged into one of the computer’s USB port.

Once you are signed into the computer and have the Windows Desktop open, find the icon labeled

“Microscope Image Capture” (AmCap) and open it.

Once MicroScope Image Capture (AmCap) has opened, you should be able to see the preview from the

microscope already showing in the window. If NOT, go to end of this doc for settings verification.

While previewing the live image, move the slide around to your desired location for your image capture.

Once you have the desired screen depicting the image you want to capture, you will need to use the

Microsoft Snipping Tool.

While reviewing the image, move the slide as necessary or adjust

focus to get desired view for video capture. Follow steps on next

slide

Video Capture from Scope: Camstudio Startup

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1. Go to Start/Win button and select

Camstudio under the Engineering Folder

2. In the Camstudio application, select the

“red” start button

Video capture from Scope; Camstudio; Record, Save

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1. After selecting the “red” record button,

Camstudio will request that you select the

Window to be recorded.

2. Select the AMCAP window that is currently

displaying the image/video from the Scope

3. When done with the recording, select the

“blue” stop button to halt video capture

4. Following Camstudio menus to save the

video stream .avi file to a folder/flashdrive.

Image Capture: Scope to PC, Camera Svideo to USB input

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Belkin F5U228 Hi-Speed USB 2.0 DVD Creator

From Scott Schneider 9/4/11.

Product Features

Product Type - DVD Creator

- Lets you share your video creations on tape, DVD,

VCD, via e-mail, or the Web

- Creates DVDs to play on your home or on your PC

DVD system

- Supports CD- and DVD-burner hardware

- Complies with Hi-Speed USB specifications

Technical Details

Brand Name: Belkin

Model: F5U228

Hardware Platform: PC

Form Factor: External

Weight: 0.45 pounds ,

Price: List $100, discounted via Amazon, ~ $55.00

http://www.amazon.com/Belkin-F5U228-Hi-Speed-USB-Creator/dp/tech-

data/B00008KIUI/ref=de_a_smtd,

Scanning;

Black/White/Transparency

SOP

For Use at BRDG Park

Using Densitometer Scanner and Software Application

Prepared by: Bob Morrison

Instrumentation Specialist

Revised Jan 2014 STLCC_CPLS;Morrison 10/20/2014 Page 21

Scanning: @BRDG using Densitometer Scanner

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1. Turn on Densitometer/Scanner and

wait for both lights to show steady

green- this is the ready state.

2. Turn on Host computer, logon as

Operator/SMET after setting the

domain to “this PC”, FVBP126B-01

3. Select Quality One application to

scan (acquire) images

Scanning: Quality One-Select Scanner

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1. Choose Select Scanner on the

Volume Quick Guide menu

2. Select GS-800 as scanner type

Quality One Application

Startup Screen

Scanning: @BRDG, Drag to Enclose Image to be Scanned

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Drag Box to Enclose

approximate Image area

Scanning: @BRDG, Select Photograph, then Preview scan

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1. Select to identify image type

2. Use Photograph for most applications

3. Select Dark Bands = Positive

Scanning: @BRDG, Preview Scan

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1. Select “Preview Scan “ to activate scanner

2. Wait for Window to complete

3. Adjust “Crop Box” to image area for scan

Scanning: @BRDG, Acquire Image

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1. With “Crop Box” enclosing area of

interest,

2. Select Acquire Scan, note do not open or

move image source until this operation is

completed.

Scanning: @BRDG, Select Preview Scan, Wait for Image to Appear

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1. Review “Raw Image” in pop-up

window

2. Do not save in this format unless you

are running Proteomics or other

analysis application next

3. Proceed to next Slide to “Export as jpg”

Scanning: @BRDG, Export as JPG or Tif File

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1. Select File from Top Toolbar, then

2. Select Export to JPEG Image for most

applications, small file size

3. Select Export to TIFF for higher or

publication quality image. This allows

more control of quality if needed.

Scanning: @BRDG, Select Location for JPEG Image

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2 .Locate Flashdisc or other Folder to save image

3. Note, On Proteomics or Densitometer use, select “Persistent Storage Z”

4. Repeat steps to scan additional images

5. Exit Application and turn off Densitometer when finished

1. For JPEG, Select Export, Adjust

Quality if desired

2. For TIFF, Select Publication

option and adjust quality and

file size as desired

Scanning; Color or

Black/White

SOP For Use at BRDG Park

Using UMAX Scanner and Software

Prepared by: Bob Morrison

Instrumentation Specialist

April 2014 STLCC_CPLS;Morrison 10/20/2014

Page 31

STLCC_CPLS;Morrison 10/20/2014 Page 32

Scanning: Color and Black/White using UMAX MagicScan

1. Turn on Densitometer/Scanner and wait for both lights to

show steady green- this is the ready state.

2. Turn on Host computer, logon as Operator/SMET after

setting the domain to “this PC”, FVBP126B-01

3. Place items to be scanned on the glass surface, face down

4. From the Start Menu, Select Programs, then Magicscan

Folder, and Magicscan application

Scanning: Color, UMAX-Magicscan, Select Quality

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1. Drag the corner of the scanning box to encompass the entire or

portion of the glass where you photos are located.

2. Note, the PREVIEW option does not work so make sure the box is

large enough to accommodate your total image.

3. Select the quality of scan you need, 100-150dpi

is normally good enough for most color

photos.

Scanning: MagicScan, Select/Set Location of Image File/Type

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1. Select the Folder Icon, then navigate to the

location on hard or flashdrive to place scanned

images. Do this on each scan operation.

2. Select the image type, JPEG is recommended.

3. DO NOT SELECT PREVIEW Option

4. Select SCAN and wait for the scanner to respond

and sweep the images .

Scan

Preview

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1. After scanner sweeps, the image is stored in the

location specified earlier and you are ready for

another scan

2. DO NOT try to use the Preview option, it will fail

and abort the entire process.

Preview

Camcorder/Camera: Canon Vixia HFM31@ BRDG (Model: HF M31 A KIT , SN:152050361653, date received 9/9/10)

STLCC_CPLS;Morrison 10/20/2014 Page 36

The Canon VIXIA HF M31 Dual Flash Memory

Camcorder features a 32GB internal flash drive and

an SD memory card slot in an ultra-sleek, compact

and lightweight body - it's a brilliant blend of capability

and convenience. Canon's advanced imaging

technologies, including a Genuine Canon 15x HD

Video Lens and 3.89-megapixel Full HD CMOS

Image Sensor, help to deliver high definition video

and 3.3 megapixel photos with incredible detail and

lifelike color. Sophisticated features such as Smart

Auto "reads" the visual components of the scene

you're shooting and chooses the best settings. Touch

& Track allows you to achieve sharp focus and

precise exposure for any subject, simply by touching

the 2.7" Touch Panel LCD. And to complete the

entertainment picture, 5.1-Channel Surround Sound*

ensures your audio is every bit as impressive as your

video.

Flash Memory

Stunning Image Quality

Dynamic SuperRange

Optical Image Stabilization with Powered IS Instant AF

2.7" Touch Panel LCD

Screen Smart Auto Touch & Track

Genuine Canon Face Detection

Advanced Video

Snapshot Easy Web Sharing 3.3 megapixel Camera

24p & 30p Frame Modes

Terminals Audio Compatible with Optional WP-V2

Waterproof Case

Scandisk 16gb disk (extra)

Hotlink to Canon HFM31 Manual (249 pgs) ….pdf

Image Capture: Camcorder, Canon,

Dual Mode (photo or video) Basic Operations

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Image Capture: Camcorder; Canon, Parts, Left-Right Sides

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Image Capture: Camcorder, Canon; Parts-Top View, LCD

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Image Capture: Camcorder, Uploading Photos to PC

• Uploading photos to a PC

– Plug USB cable to port under flap by strap handle and to PC

– Turn on the Camera, Open the Viewing/LCD screen and select “Connect

to Computer” option

– Wait for a Microsoft Wizard to start on the PC and follow the menus to

upload photos to any folder: Note if the wizard does not start, use the

“My Computer” option to locate the internal memory and disk for the

camera.

– Use the “Safely Remove Device” option in the bottom tray on the PC to

stop the Camera USB connection

– Remove USB cable and turn off the PC

STLCC_CPLS;Morrison 10/20/2014 Page 40

Image Capture: Uploading Videos, Primary and Alternative

• Uploading Videos, Standard Method

– Install Pixella Imagemixer Software on a networked PC

• Install Disc 1 and 2 per normal Windows options

• Turn on Camcorder , set to video review mode, select Computer/Printer USB

connection

– Connect Camcorder to PC via USB

• Menu should appear on camcorder screen ; choose Computer/Printer (not burn DVD)

– Imagemixer app will startup automatically (if not use icon).

• Alternative (if Pixella wizard does not start automatically)

– Install camera to PC USB link

– Copy camera MTS files found in BH stream folder to desktop folder IMxSEVer6 (location

available to Mixer s/w)

– Starte Videomixer app , chose File, Add Files to Library

– Navigate to IMxSerVer6 folder and located file recently copied

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Image Capture: Canon Camcorder, Upload and Publish Video

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1. Import High quality video

files (Canon format) from

camcorder to an album folder

in Pixella

2. View and Edit video files ,

names, description, etc.

3. Upload video files to

Youtube and convert them to

standard formats for

publishing. (Requires Youtube

and/or Google account)

Hotlink to

Pixella

Manual 161

pgs….pdf

Image Capture: Camcorder,Import Video, Select Device

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1. Select location of files

within the camera or other

source

2. Select which files to import

or ALL

Image Capture: Camcorder, File Mgmt and Edit

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1. Select Video File Mgmt and

Edit to review imported

files and existing database

Image Capture: Camcorder, File to Edit or Clip

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1. Highlight file to edit or clip,

2. Select Move Edit

Image Capture: Camcorder, Edit Movie

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1. Select Edit New Movie or

open and older project that

is in work

Image Capture: Camcorder, Set Resolution

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1. Select Resolution of final

product, Default is

1440x1080

Image Capture: File Mgmt, Storyboard Mode

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Image Capture: Camcorder, Edit/Clip Segments, Step 1

1. In Storyboard or Timeline view mode,

highlight the original desired video

2. Right click on that file to drop down

menu

3. Select Trim Multiple Ranges

Image Capture: Camcorder, Edit/Clip Segments, Step 2

1. Drag right-side bookend to

the end of the segment you

wish to clip

2. Drag left-side bookend to

the start point of the

segment to be clipped

Image Capture: Camcorder, Edit/Clip Segments, Step 3

1. Select the Clip symbol

2. Then Select OK

Image Capture: Camcorder, Edit/Clip Segments, Step 4

1. Shorter clipped segment

will show up in the library

2. Select Play arrow to review

the segment before saving

Image Capture: Camcorder, Edit/Clip Segments, Step 5

1. Select Save to store the

clipped segment with other

raw and/or clipped

segments in the Camcorder

Library

Image Capture: Camcorder, Edit/Clip Segments, Step 6

1. Enter filename for the

clipped segment, then

SAVE

Image Capture: Camcorder, Export to PC/Flashdrive,Step1

STLCC_CPLS;Morrison 10/20/2014 Page 55

1. Highlight segment/clip to

export,

2. Select FILE in top taskbar

3. Select Export

Image Capture: Camcorder, Export Clip, Step 2 Whereto

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1. Browse to location for

exported file: PC Folder,

Flashdrive, other

2. Select Export to start

process

Image Capture: Camcorder, Export to Flashdrive Step 3

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Image Capture: Exporting to Flashdrive, Final Step

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Image Capture: Camcorder, Upload to YouTube, Edited Files

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Image Capture: Camcorder, Select Files to Upload

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Image Capture: Camcorder, Select Upload Quality

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Image Capture: Enter Google ID/Password

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Image Capture: Camcorder, Writing to DVD/Disc

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Image Capture: Camcorder, Writing to Disc, Quality

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Image Capture: Camcorder, Canon; Interface Options

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