Post on 05-Dec-2014
description
Internship surgery
’73 - ’83 ’84 ’90 ’91 ’92 ’93 ’94 ’95 ’96 ’97 ’98 ’99 ’00 ’01 ‘03 ‘05 ‘07 ‘09 ‘11
Children’s Hospital of Eastern Ontario experimental spinal cord injury (SCI)
Neurobiology Res Ctr QC Res Ctr in Science & Macromol Eng biomaterials concept for neuroregeneration
Keele University polymer drug delivery
Prague Institute of Macromol Chemistry bioactive hydrogel formulation & dev.
Quebec Biomaterial Institute adjunct faculty invention of Neurogel as novel approach to SCI
Organogel Canada Ltd
Organogel Canada Ltd
CERMAV-CNRS GIN-Inserm program directorbioretina glycosylated hydrogels neural stem cell recruitment
France Canada uk Czech Rep. Canada France
EMPLOYMENT
Areas represent relative energy expenditure over timeRESUME/ROADMAP INFOGRAPHIC Stéphane Woerly MD PhD
Medical Research Council Fellowship
ACADEMICS
Pie slices represents approx. percentage time investment
graduation
MD training neurochemistry
graduation
Polymeric biomaterialsNeurodegenerationNeuroregeneration
physical-chemistrypolymerization processHydrogel chemistry
CSOexperimental surgery preclinical proof-of-concept on established SCI models
creation of Organogel Canada LtdQuebec Metro High Tech Parkfundraising/IP development
CMOclinical master file new treatment option to SCIendogenous regenerative technology
Neuro polymer professional
Neurogel development
(biomaterials ‘90)
Macroporous pHPMA Hydrogel
© Copyrights Woerly
A proprietary innovative technology platform with applications in Tissue Neuro-Engineering and RegenMed
A proprietary innovative technology platform with applications in Tissue Neuro-Engineering and RegenMed
Tissue-building properties
of pHPMA hydrogel
New AstrocyticTissue Matrix
Axonal Growth
Angiogenesis
Signaling moleculecirculation
Stem cell recruitment
EM Structure imaging of HPMA EM Structure imaging of HPMA hydrogel hydrogel macro/mesoporesEM Structure imaging of HPMA EM Structure imaging of HPMA hydrogel hydrogel macro/mesopores
Cross-linked poly[N-(2-hydroxypropyl) methacrylamide] hydrogel showsa macromolecular architecture with a colloidal-type structure
3D section plot
Volume viewer
EM Structure imaging of HPMA EM Structure imaging of HPMA hydrogel hydrogel microporesEM Structure imaging of HPMA EM Structure imaging of HPMA hydrogel hydrogel micropores
Micropores on the surface of microgels that increase the surface area of the bulk of the hydrogel network
in vivo confocal imaging of in vivo confocal imaging of hydrogelhydrogelpolymer/CNS tissue interface
Polymer-tissue interfacePolymer-tissue interface
CNS tissueCNS tissue
in vivo confocal imaging in vivo confocal imaging (I)(I)immuno-labeling of stem cell migration within the hydrogel intracerebral implantation (4 month post-grafting)
in vivo confocal imaging in vivo confocal imaging (I)(I)immuno-labeling of stem cell migration within the hydrogel intracerebral implantation (4 month post-grafting)
in vivo confocal imaging (II)in vivo confocal imaging (II)immuno-labeling of stem cell migration within the hydrogel intracerebral implantation (4 month post-grafting)
in vivo confocal imaging (III)in vivo confocal imaging (III)immuno-labeling of stem cell migration within the hydrogel implanted in the spinal cord (17 month after trauma and post-grafting)
neurofilament/MBP (red) Nestin (green)/MBP(red)
The arrow and the insert show a NF+ neuronal cell body
in vivo confocal imaging in vivo confocal imaging nerve fibres growth within the hydrogel pore network
in vivo confocal imaging in vivo confocal imaging nerve fibres growth within the hydrogel pore network
3D hydrogel volume reconstruction 3D hydrogel volume reconstruction cell distribution (red dots) within the pore structure
in vivo confocal imaging in vivo confocal imaging angiogenesisin vivo confocal imaging in vivo confocal imaging angiogenesis
Growing blood vessels within the polymer network
in vitro SEM imaging in vitro SEM imaging HPMA hydrogel including human neuroblastoma cells
in vitro SEM imaging in vitro SEM imaging HPMA hydrogel including human neuroblastoma cells
Neurite extending within the polymer
network
Neurite extending within the polymer
network
Cell aggregates Cell aggregates
in vitro nestin-hoescht imaging in vitro nestin-hoescht imaging hydrogel including primate neural precursor cells *
* source: Stem cell and Brain Research Institut of Lyon
Polymer networkPolymer network
Key features of NeurogelKey features of Neurogel
• Elastic modulus matches that of CNS
• Tensile strength across the area of transplantation
• Porosity allows cells, growth factors and axonal elongation to fill the lesion site
• Does not breakdown—allows for enough time for regeneration
• Promotes the exclusion of scar tissue and Wallerian degeneration in the transected spinal cord
• flexible platform for the optimization of scaffold functionality
• Good surgical procedure feasibility
Current developmental statusCurrent developmental status
• Laboratory prototypes• Product Requirement Specifications• Verification & validation• Preclinical Proof-of-concept (SCI)• Toxicology/biocompatibility testing• Sterility assurance• Packaging & stability issues
Neurotechnology / proof-of-principle
HPMA hydrogel graft acrossa lesion of the spinal cord
Gross anatomy of theimplantation site 15 months post-surgery
Introduction of a sialyl derivative group (immunolabeled in green) to create specific biological properties
Functionalization of HPMA hydrogel