visualizing Gel Electrophoresis

flatbed scanner+

UV transilluminator

gel stained with Biotium GelRed

LED light guide (FTIR?)

linear CCD array

LED light guide (FTIR?)

linear CCD array

linear CCD array, upside down

RGB LED (3 diodes)

RGB LED couples here

red solid state laser light through light guide rail

welding curtain: UV filter

fitting UV curtain over

CCD array

FOTODYNE UVB transilluminator (circa 1994)

glass (UV-opaque)

special UV-transparent polycarbonate

stained gel

UV-glass(visible-opaque)

transilluminator

glass (UV-opaque)below gel

welding curtain on top of gel

365nm handheld lamp

exposure: 1/4 sec

exposure: 5/8 sec

exposure: 1 sec

exposure: 2 sec

exposure: 4 sec

exposure: 8 sec

contrast enhanced

365nm handheld lamp

~365 nm

visualized w/ ambient light

visualized w/ ambient light

visualized w/ UV light from fotodyne

imaged w/ Lumix GF1 SLR cameraimaged w/ modifed scanner

darklight!

using small UVB lamp inside the scanner

next steps:add low-cost filter to linear CCD

GelGreen:Excitation Peak: ~500 nmEmission Peak: ~540 nm

Need to filter emission light before linear CCD- find a filter just above the light source but below the emission- 470-510 nm < filter < 540 nm- TAPR50 sheets filter everything below 528 nm

get TAPR50 528nm filter sheets($35 / 10 sq feet)

http://bit.ly/TAPR50