Figure S1

A

WM

1361

A

NRAS mutant

IKKε

Actin

WM

1346

WM

3670

WM

1366

WM

852

SK

Mel

2

SB

cl2

RA

W24

6.7

SK

Mel

173

SK

Mel

30

Figure S2

A - #2 - #2

WM1366 WM1346

B

C

0

***

*

20

40

60

80

100

WM1346 WM1361A WM1366 SBcl2

Per

cen

t M

igra

tion

of C

on

tro

l siCTL

siTBK1 #2

**

*

*

0

20

40

60

80

100

WM1346 WM1361A WM1366 SBcl2

Per

cen

t In

vasi

on

of

Co

ntr

ol siCTL

siTBK1 #2

siCTL

siTBK1

siCTL

siTBK1

ERK

TBK1

siTBK1

Figure S3

0 0.01 0.1 1 5

Aurora A Thr288Aurora B Thr222Aurora C Thr198

Aurora B

WM1366 TR TBK1-myr

AZ909 (μM)

IKKε

PDK1

phospho-PDK1 Ser241

Aurora A

0 0.01 0.1 1 5

SKMel2

phospho-Aurora A/B/C

Figure S4

AZ909AZD6244

- - + +- + - +

WM1366

siTBK1AZD56244

- - + +- + - +

WM1366

Bim-EL

Mcl-1

Bim-EL

Mcl-1

Bmf Bmf

BA C

siCTL

siTBK1

siCTL

siTBK1

AZD6244 DMSO