Development of a Murine Perfusion SystemNicola Asgill

Fredrick Hilliard

George Kittos

Objectives

Langendorff perfusion systemRequirements:

Miniature scale Fluid temperature controlled Inter-changeable components Drug introduction into perfusate

Background

Langendorff Perfusion SystemSimulates natural blood flowRetrograde fluid flow

Attached to aorta

Examine effects of drug on heart

Considerations for project

Why will this work? Controlled temperature, flow rate and pressure

Improvement of heat stability Multi-drug delivery capability

Disposable components

Why is this important? Possible testing applications:

Drug delivery Cardiac cell response

Prototype

Prototype Modifications for Preliminary Testing Flow will be gravity controlled

Removal of Peristaltic Pump

Removal of Flow Sensor

Pump and Sensor will be added based on results of preliminary testing

Peltier Element

Max Current 7.6A

Max Power 76 W

Max Voltage 16.3 V

dT Max 72°C

Dimensions 48x48x4.8mm3

Peltier Element Test

GoalsShow functionality – Heating capabilitiesCalculate Time Constant

To heat from room temperature to 37 ± 1°C

Determine appropriate input voltage

Peltier Element Test Conclusion:

Input Voltage of 8V – longer time to heat sample, needs to be faster to perfusate at constant temp.

Input Voltage of 15V – faster heating, but found that this voltage will cause the device to fail.

Input Voltage of 12V – faster heating and the device did not malfunction

LabVIEW Temperature Control

Transparent Glass Chamber

Plexiglass

Visualize electric activity in heart using dyes

Staggered holes

Transparent Glass Chamber

Current Work Heating Perfusate to 37 ± 1°C

Manufacturing Plexiglass chamber

Verify and Validate the LabView Module for controlling and maintaining constant temperature

Tygon Tubing

Peltier Element

Future Work

Acquiring additional components

Assembling Prototype

Testing the perfusate heating apparatus

Design and Test Control Valve System

Harvesting the Mouse Heart

Thanks

Questions?