Development of a Murine Perfusion System
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Development of a Murine Perfusion SystemNicola Asgill
Fredrick Hilliard
George Kittos
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Objectives
Langendorff perfusion systemRequirements:
Miniature scale Fluid temperature controlled Inter-changeable components Drug introduction into perfusate
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Background
Langendorff Perfusion SystemSimulates natural blood flowRetrograde fluid flow
Attached to aorta
Examine effects of drug on heart
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Considerations for project
Why will this work? Controlled temperature, flow rate and pressure
Improvement of heat stability Multi-drug delivery capability
Disposable components
Why is this important? Possible testing applications:
Drug delivery Cardiac cell response
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Prototype
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Prototype Modifications for Preliminary Testing Flow will be gravity controlled
Removal of Peristaltic Pump
Removal of Flow Sensor
Pump and Sensor will be added based on results of preliminary testing
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Peltier Element
Max Current 7.6A
Max Power 76 W
Max Voltage 16.3 V
dT Max 72°C
Dimensions 48x48x4.8mm3
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Peltier Element Test
GoalsShow functionality – Heating capabilitiesCalculate Time Constant
To heat from room temperature to 37 ± 1°C
Determine appropriate input voltage
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Peltier Element Test Conclusion:
Input Voltage of 8V – longer time to heat sample, needs to be faster to perfusate at constant temp.
Input Voltage of 15V – faster heating, but found that this voltage will cause the device to fail.
Input Voltage of 12V – faster heating and the device did not malfunction
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LabVIEW Temperature Control
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Transparent Glass Chamber
Plexiglass
Visualize electric activity in heart using dyes
Staggered holes
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Transparent Glass Chamber
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Current Work Heating Perfusate to 37 ± 1°C
Manufacturing Plexiglass chamber
Verify and Validate the LabView Module for controlling and maintaining constant temperature
Tygon Tubing
Peltier Element
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Future Work
Acquiring additional components
Assembling Prototype
Testing the perfusate heating apparatus
Design and Test Control Valve System
Harvesting the Mouse Heart
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Thanks
Questions?