Post on 07-Feb-2017
05/01/2023
Bioprocess Technology
Dr.D.Surendra Nadh Benarji M.Sc, M.Phil, Ph.D (Biotechnology)
E-Mail: dsnbenerji@gmail.com Mobile: +91 9247407189 Acharya Nagarjuna University, Nagarjuna Nagar, Guntur Andhra Pradesh, India
05/01/2023
Sewage Treatment
Sewage Treatment Biological
Chemical
Physical Filters, CentrifugationRadiation
Spirulina, LactobacillusBiopolymer (Chitosan)
Enzymes,
05/01/2023
Bread Production
Yeast Culture
Malt of Yeast Cells
Bread BakingBread with cheese
MaltingYeast Cell’s
SCP
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Cheese Production
Heat milk at 85 oC
Allow milk to cool at 24
oC
Add starter culture(Lactic acid Bacteria) Add Rennet
Milk Coagulation
(4 h)
Place the curd in
cheese cloth
Separate the Whey from the curd
Dry the CurdAnd
Add NaCl
Cut the curdInto pieces
Store the curd
Cut the curdInto pieces Cheese
Lactic acid Cheese
05/01/2023
Penicillin Production
Penicillin notatumPenicillin chrysogenum
Penicillin Biosynthesis
Penicillin G: R Variable chain
Alexander Fleming
Valine Cysteine
05/01/2023
Vitamin B12 Production
Streptomyces griseus
Culture
Purification
Crys
talliz
ation
05/01/2023
Biogas Production
Animal Dung
Anaerobic Digestion
By Methanogens
Methane
UtilityGreen HouseGreen Globe
05/01/2023
Bio-Fertilizers
Industrial WasteBiomassDomestic waste
Animal Waste
Fertilizer
Earthworm
Biofertilizers - Vermi Compost
Nitrogen Fixation
Symbiotic Asymbiotic
In Vivo
05/01/2023
Biodiesel Production
Aspergillus niger
Lipol
ytic e
nzym
es
Biofuel Production
GasoholMotor VehiclesEcofriendlyCarbon dioxide
And Carbon monoxide free
CarbohydratesEthanol
YeastPetrol GasoholSugar Cane
Molasses
Green House
Invertase
Zymase
05/01/2023
Insulin Production (Hormone)
3’5’
5’3’
A Chain
B ChainChimera-A Chimera-B
C
N C
N
Insulin
Insertion
Hybrid-BHybrid-A
Clones-BClones-A
Culture
Isolation
Insulin-A chain Insulin-A chain
In-Vitro
05/01/2023
Growth Hormone Production
5’3’Growth
HormoneGene Chimera
N C
Insertion
Hybrid
Clones
Culture
Isolation
Growth Hormone
polypeptide
E.coli
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5’3’
INTEFERONGene
Chimera
N C
Insertion
Hybrid
Clones
Culture
Isolation
Interferon(Glycoprotein)
Interferon Production- Antiviral Protein
05/01/2023
DNA Amplification-(PCR)
Melting at 94 oC
3’5’
5’3’
Denaturation
5’3’
3’5’
Annealing Primers at
50 oC
5’
5’
5’3’
3’5’
Extension72 oC
5’3’
3’5’
5’3’
3’5’
DNA Copies
Double Stranded
DNA
Melting - SSB Protein, Helicase , Topoisomerase
Primer – Primase
Polymerization - TaqDNA Polymerase, Mg, ATP’s
DNA Ligation - DNA Ligase
05/01/2023
Transgenic Plants
Tissue culture
05/01/2023
Protein Engineering
Vector
YFG-DNA Chimera
Proteins
CloneClones
Isolation of Desired DNA
05/01/2023
Anti-Venom
Snake Venom
Snake
Serum
AntiserumAnti Venom
Horse
Extraction
Stored at 20 oC
05/01/2023
Molecular Farming
Microinjection
Blastula
MorulaAnimals
DNA Chimera
MILKANTISERA
Metabolic Engineering
Metabolic Engineering
TNF
05/01/2023
Cybrid Technology
Live Stock Production
05/01/2023
Monoclonal Antibody Production
Oil
05/01/2023
Toxoid Production
Bacteria BacteriaCulture
Toxin Toxoid
Extraction
FormaldehydeTreatment
Purification
Toxoid
05/01/2023
Gene Therapy
DNA Removal of Defective DNA
YFG@DNA
InsertionDisease FreeHuman
Adenosine De-aminase
Increase Immunity
05/01/2023
Edible Vaccines
Agrobacterium mediated gene
transfer
05/01/2023
DNA Vaccines
Vaccination
Plasmid
Insertion
Clone AntibioticResistant
Culture
05/01/2023
Enzyme Linked Immunosorbent Assay
05/01/2023
Molecular Labeling- A Method
DNA
P32
DNA Analysis
Protein
S35
Protein Analysis
Organic Molecule
Organic Molecule
OrganicMolecule Analysis
C14
05/01/2023
Molecular Mechanism's of Biosystem
DNA Replication
Splicing
Gene Expression
Methylation
Hybridization
Ligation
05/01/2023
Human Genome Project
Karyotype
MALE FEMALE
DNA
MetabolomicsGenomics
Proteomics
Disease Free Human
TTGACA
TATAAT
Unwind DNA
Initiate Transcription
Promoter
05/01/2023
In Vitro Fertilization (IVF)
(1). Micro injection of DNA into egg
(2). 2 cell embryo
(3). 4 cell embryo
(4). Blastocyst
(5). Morula
05/01/2023
Stem Cell Technology
Stem Cell
Healthy Human’s
Neuron
Bone Marrow
Neuron Tissue
Insertion of Stem Cell
HO
HOH2CCH2
NH3+
B-cell’s of Pancreas
secretes Insulin
Dopamine
3-4 days Embryo
05/01/2023
Ribosome-Antibiotic Interactions
Venkatraman Ramakrishnan Ribosome
Thomas A Steitz Ada E Yonath
Antibiotic Resistance in Humans
Antibiotics
Drug DesignMolecular Docking
Homology Modeling
Drug DeliveryToxicity
Prediction
Bioinformatics
05/01/2023
Analytical Method Development
Electron Microscope
Shape of cell’s and cell organells
Concentration and Identification of
molecules
Mass Spectrophotometer
X-Ray Crystallography
Sequence of DNA and Proteins
Fermenter
HPLC(LC-MS)
Structure of the Molecules or
Organells
NMR
Gas Chromatography
(GC-MS)
Fermentation
Structure
Concentration and Identification of
Molecules
Microbial, Animal and Plant cells.
DNA and Protein
Cell’s, Cell organells, electron
Organic molecules
Organic molecules
Proteins, Enzymes, Acids, Fuels
Organic molecules
Electrophoresis Identification DNA, Protein
Gel Filtration Separation Proteins
PCR Amplification DNA, C-DNA, mRNA
Centrifuge Separation DNA, RNA, Proteins, Cell organells
05/01/2023
Nano-Technology
Nano Carbon
Medicine
ParticleCarbon Nano Tube
Reduce Environmental
Pollution
ReduceAcid Rains
Nano- Medicine
Rapid CureOf
Disease
05/01/2023
Disease Diagnosis – Silicon Chips
Micro Array
RNA Array
Protein Array PUQEGHILDCLKSTMFEWCUWECFP
UCAGUCAAGAGUAAGUAGCUAUCA
DNA Array TCGTCCATTATCCAGTCCATTAAGTC
Array Sequence
RNAor
DNAor
ProteinLabeling
on Silicon Chip
SiliconChipDisease Diagnosis
Elephantiasis
Malaria
Syphilis
HIV
Hepatitis
Inborn Errors
Protein Expression systemand
Gene Expression system
In SilicoGSP
05/01/2023
Next Generation Sequencing
Sanger’s DNA Sequencing
DNA Fragment DNA Sequence
Mass Spectrophotometer
DNA or ProteinFragment
DNA or Protein Sequence
Genomics or
Proteomics
GENOME Sanger’s DNA Sequencing
GENOME DATA
NEXT GENERATION
DNASEQUENCING
MutationsCancerCloning DATA DNA Editing
05/01/2023
Bioinformatics
Next Generation Sequencing (NGS)
Genome
Genome Data Analysis
GlobalizationFinger Prints
(Dactylogram)Disease FreeUniqueDNA
Plantation
Save Our Soul DATA(SOS DATA) Genomics Proteomics
Metabolomics
DNA Editing
RNAi
05/01/2023
Organ Transplantation and Organ Culture
MHC - HLA Typing HLA Matching
Organ Transplant
Graft Rejection
Stem Cell
Retina Culture Ear Culture Skin Culture
05/01/2023
Statistical Analysis
Probability
Average
Regression
ANOVA
Correlation
Factor
Frequency
Optimization
Cluster
RSM
Experiments
Design
DATA
RSM
Experimental Results
In Vivo
In Vitro
In Silico
In Situ
05/01/2023
Global Positioning SystemBrain’s Positioning System
Mice
Memory Motion Function
Human Brain
GPS
Edward I Moser
May Britt Moser
John O Keefe
05/01/2023
Precaution’s(1). Wear apron while conducing Experiments in bio-laboratory
(2). Aseptic and sterile conditions while conducting experiments
(3). Preserve food and water in glass ware
(4) . Avoid direct contact of microorganisms
(5). Use sterile glassware while conducting experiments
(6). Maintenance of sterile and aseptic conditions in laboratory
(7). Use disinfectants to was hands after the completion of experiments
(8). Remove footwear outside while enter into the laboratory
05/01/2023
Bioprocess Ethic’s Bioprocess should be carriedout under sterile and aseptic conditions. The Materials and tools used in the bioprocess must have certification by
authorized. The products formed in the bioprocess should be beneficial for the
Humans, Plants, Environment and other Animals. The bioprocess should be carriedout under expert supervision. The process developed under expert supervision must be knowledgeable to
Academician’s, Researcher’s and Student’s. The bioprocess developed by technologies should be pollution free
technologies. The experimental procedures of In Vivo, In Vitro, and In Silico methods
should be compatible with biological systems. The Materials, Methods and the Products should be Ecofriendly.
05/01/2023
Science Quotes• All that is valuable in human society depends upon the opportunity for development accorded the individual
- Albert Einstein• The greatest barrier to success is the fear of failure.- Sven Goran Eriksson• The joy of discovery is certainly the liveliest that the mind of man can ever feel.” – Claude Bernard• What’s in a name? that which we call a rose by any other name would smell as sweet- William Shakespeare • Every organic being is related, in the most essential yet often hidden manner, to that of all other organic
beings, with which it comes into competition for food or residence, or from which it has to escape or on which it preys- Charles Darwin
• Science knows no country, because knowledge belongs to humanity, and is the torch which illuminates the world- Louis Pasteur
• The pure culture is the foundation of all research on infectious disease- Robert Koch• The remarkable capacity of the immune system to responds to many thousands of different substances
with exquisite specificity saves us all from certain death by infection-Martin C. Raff• Everything is everywhere , the environment selects- M.W. Beijerinck• The recombinant DNA breakthrough has provided us with new and powerful approach to the questions that
have intrigued and plagued man for centuries- Paul Berg• Science is built up with facts, as a house is with stones. But a collection of facts is no more a science than a
heap of stones is a house - Jules Henri Poincaré• Keep working hard and you can get anything that you want." -Aaliyah• Failure's hard, but success is far more dangerous.“-Po Bronson• "The greatest barrier to success is the fear of failure." - Sven Goran Eriksson
05/01/2023
'Paleoanthropology' means 'Ancient' (from Greek 'palaeos') man as in humanity (from Greek 'anthrōpos') and study or
discourse (from Greek '-logia').
05/01/2023
Mail: dsnbenerji@gmail.com