Post on 05-Jan-2016
description
Automatic functional transcriptomic annotation
Bioinformatic school CIBA
Manuel Ruiz (CIRAD)
Alexis Dereeper (IRD)
Marilyne Summo (CIRAD)
Transcriptomic data
SignalGenomic expression changes
expressed proteinsTranscriptomic analysis => functions
adaptation
Transcriptomic analysis
High-throughput sequencing :
=> Higher quantity of data. => Lesser cost.
Problematic:
* How to store data ? * How to analyse data ?
Sanger Pyroséquençage 454
1000 seq. 500 000 seq.
Available tools
ESTtik :
« Expressed Sequence Tag Treatment and Investigation Kit »
• Automatic transcriptomic annotation package
• Can manage 454 data
• Integrate the analysis in a complete database and request web interface.
http://esttik.cirad.fr
454 data
Transcripts
Random cutting 200 – 800 bases
454 sequencing => ~380 bases
Transcript contig assembly
Consensus sequence