Automatic functional transcriptomic annotation Bioinformatic school CIBA
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Automatic functional transcriptomic annotation Bioinformatic school CIBA Manuel Ruiz (CIRAD) Alexis Dereeper (IRD) Marilyne Summo (CIRAD)
description
Automatic functional transcriptomic annotation Bioinformatic school CIBA. Manuel Ruiz (CIRAD) Alexis Dereeper (IRD) Marilyne Summo (CIRAD). Transcriptomic data. Genomic expression changes. Signal. expressed proteins Transcriptomic analysis => functions adaptation. - PowerPoint PPT Presentation
Transcript of Automatic functional transcriptomic annotation Bioinformatic school CIBA
Automatic functional transcriptomic annotation
Bioinformatic school CIBA
Manuel Ruiz (CIRAD)
Alexis Dereeper (IRD)
Marilyne Summo (CIRAD)
Transcriptomic data
SignalGenomic expression changes
expressed proteinsTranscriptomic analysis => functions
adaptation
Transcriptomic analysis
High-throughput sequencing :
=> Higher quantity of data. => Lesser cost.
Problematic:
* How to store data ? * How to analyse data ?
Sanger Pyroséquençage 454
1000 seq. 500 000 seq.
Available tools
ESTtik :
« Expressed Sequence Tag Treatment and Investigation Kit »
• Automatic transcriptomic annotation package
• Can manage 454 data
• Integrate the analysis in a complete database and request web interface.
http://esttik.cirad.fr
454 data
Transcripts
Random cutting 200 – 800 bases
454 sequencing => ~380 bases
Transcript contig assembly
Consensus sequence
