A close look at

BionanotechnologyMCCB

October 21 2011

Thomas L. Deits, Ph.D.

What is nanotechnology?

Any process or product that involves analysis or manipulation of materials at the nanometer scale

A thumbnail introduction to nanotechnology

Your fingernail is about as thick as 500 E. coli,

each of which is as thick as

500 1- nanometer slices

E. coli with Pili and FlagellaAng L National University of Singapore

http://www.icmm.csic.es/spmage07/spmageview.php?id=50

Why is the nanometerscale important?

Virtually every property of matter that we care aboutarises at the nanometer scale:

ColorOdorStrengthFlexibilityConductivityDurabilityBiological activity

Bionanotechnology

Biology happens at the nanoscale:

DNAenzymesmusclesnervessenses

Biology is a Big Idea in Nanotechnology

• Size and Scale• Structure of Matter• Forces and Interactions• Quantum Effects• Size-Dependent Properties• Self Assembly • Tools and Instrumentation• Models and Simulations• Science, Technology and Society

The Big Ideas of Nanoscale Science and EngineeringS.Y. Stevens et al. NSTA Press 209

Capillary net of alveolae showing erythrocytes within the blood vessels.Oliver Meckes

http://www.flickr.com/photos/fei_company/5679999618/in/set-2157626382741945

Human beta-amyloid fibrils and oligomers"Prof. Francesco MantegazzaUniversita' degli studi di Milano-Bicocca

http://www.icmm.csic.es/spmage07/spmageview09.php?id=165

Human malaria Infected red blood cellsDr. Li Ang Li

http://www.icmm.csic.es/spmage07/spmageview09.php?id=30

BionanotechnologyTools and Instrumentation

How do we work at the nanometer scale?

BionanotechnologyTools and Instrumentation

How do we work at the nanometer scale?TheAtomic Force Microscope

A workhorse of nanotech

Really…

TheAtomic Force Microscope

How does it work?

Prettymuchlikethis….

How does it work?

Mss. Elizaveta Drozd. A.V. Luikov Heat and Mass Transfer Institute (Belarus)

http://www.icmm.csic.es/spmage07/spmageview09.php?id=62

What can it do?

Pre-metastasis cell aggregateINASMET-Tecnalia

http://www.icmm.csic.es/spmage07/spmageview09.php?id=25

Pyrococcus furiosus(archaea)

http://www.sciencephoto.com/media/13219/enlarge

www.nanosurf.com

And it’s not so hard to do these days….

BionanotechnologyForces and Interactions

Examples from Physiology and Biotechnology

Kodera et. al Nature 468 72-76 (2010)

Myosinwalkingvisualizedby AFM

Yves F. Dufrêne Journal of Bacteriology, October 2002, p. 5205-5213, Vol. 184, No. 19

Yeast bud scar

High speed AFMCellulase enzymes – traffic jams on the cellulose fiber

Kiyohiko Igarashi, et al. Science 333, 1279 (2011)

High speed AFMCooperation between two types of cellulases

Kiyohiko Igarashi, et al. Science 333, 1279 (2011)

BionanotechnologyTools and Instrumentation

Manipulation at the nanoscale

Nanotweezers

http://www.amir.uni-oldenburg.de/en/37099.html

Optical tweezers – nanoscale tools for biology

P. Gross, et al. Nature Physics PUBLISHED ONLINE: 22 MAY 2011 |

P. Gross, et al. Nature Physics PUBLISHED ONLINE: 22 MAY 2011 |

Optical tweezers- There’s an app for that…

R. W. Bowman, et al. J. Opt. 13 (2011)

BionanotechnologySelf Assembly

DNA Origami

Toroidally supercoiled DNADr Jozef Adamcik Ecole Polytechnique Federale de Lausanne

http://www.icmm.csic.es/spmage07/spmageview.php?id=153

DNA Origami

Depending onthe sequenceof DNA, the double helix can be bent or straight

Using the simple rules of DNA pairing, bends can be introduced by design into a DNA molecule…

DNA Origami

…making it possible to design a shape that will assemble when the right pieces of DNA are mixed.

DNA Origami

DNA Origami

DNA Origami in 3 dimensions!

Han, et al. Science 332, 342 (2011)

ES Andersen et al. Nature 459, 73-76 (2009)

DNA Origamiboxes (with lids)

BionanotechnologyStructure and Function

The Lotus Leaf

Abaxial side of a tomato leaf with trichomes.Adriaan van Aelst

http://www.flickr.com/photos/fei_company/5636849589/in/photostream

http://www.gereports.com/nanotech-breakthrough-keeps-computers-fast-by-tackling-heat/

GE announces a thermal cooling system with twice the thermal conductivity of copper and a quarter of the weight usingsuperhydrophobicity

Forepart of a miteMargit WallnerAngelika Reichmann

http://www.flickr.com/photos/fei_company/5886650891/in/set-72157626985240386

RadiolarianLinnea Rundgren

http://www.flickr.com/photos/fei_company/5730242836/in/set-72157626382741945

Sponge spiculesMiranda Waldron

http://www.flickr.com/photos/fei_company/5889773523/in/set-72157626985240386

http://www.nanotechproject.org/inventories/consumer/analysis_draft

There are over 1,000 products incorporating nanotechnology on the market today

What are they? There’s an app

for that.....

http://nnin.org

Projected job opportunities in nanotechnology by 2015 are estimated at 2 million worldwide and 1 million of those will be in the United States….

If we educate ourselves and our children to be ‘nano-savvy’

But where are the nanobots?

The nano cargo truck

Laboratory for Nanobioelectronics P.I.: Dr. Joseph Wang Department of NanoEngineering University of California, San Diego 2009

ηanoDay

Saturday in March/April 2012 (date TBD)10 AM – 4 PM

Impression 5 Science CenterLansing, MI

Nanoday 2010, Impression 5 Science Center, Lansing Michigan. Lotus leaf investigation.

Thank you!tdeits@gmail.com