Course Content Introduction to HLAs HLA Genes, alleles,proteins and antigens Structure-function of HLA molecules Genetics of the Major Histocompatibility Complex HLA Nomenclature/ HLA Dictionary Serologic HLA typing Molecular Methods for HLA typing PCR-SSP/ PCR-SSOP methods PCR-RFLP method Sequence-Based typing method (SBT) Practical sessions Clinical Impact of Molecular typing Establishing HLA laboratory Sourcing reagents for HLA typing Accreditation, Certificationof HLA lab Proficiency testing The Nigerian Bone Marrow Registry The IMGT/HLA Database HLA Laboratory software: the Orpheus, etc QC and QA in HLA lab Inauguration of the Nigerian Society for Histocompatibility and Immunogenetics (NSHI). Target Audience Medical Laboratory Scientists Medical Doctors, Pathologists, Haematologists, Immunologists Academics, Research Fellows and Scientists Pharmacists PhD Students and Post-Doctorates Venue: :Safety Molecular Pathology LaboratoryFaculty of Health Sciences & Technology, University ofNigeria,Enugu Campus, Enugu, Nigeria Date: 10-14, September, 2012for 20 participants only Registration fee ::60,000 NairaAll fees are payableto : Account Name: Safety Molecular Path Lab Services Ltd Bank: Zenith bank plc, Account Number:1012967770 Please email your Payment Teller Number toe.nna@safetybiomedical.org Further inquires: contact 07063415385 or 07034959316 or 08037435616 Or email: e.nna@safetybiomedical.org WorkshopCo-ordinator:E.Nna,PhD (Cranfield University, UK). DrNnaistheHeadofSafetyMolecularPathologyLaboratoryinNigeria, which provides HLA typing, paternity testing, human identity and cancer diagnosis.www.safetybiomedical.org The 4th Annual PCR TechnologyWorkshop: HLA Typing by Molecular Methods TheHumanLeucocyteAntigen(HLA)typingisthemostimportanthistocompatibilitytestwithparticularrelevancetoorgan/tissuetransplants.TheclinicalapplicationsofHLAtypinginclude1) typing for renal transplantation, 2) typing for haematopoeitic stem cell transplant, and 3) typing as an aid to HLA-associated diseases.HLA typing has transited from serologic typing to DNA based typing in most histocompatibility test-ing laboratories. ThisworkshopfocusesonDNAbasedtypingmethodsincludingPCR-SSP,PCR-SSOP,PCR-RFLP andSequence-basedtyping(SBT).Itisahands-onintensivecoursethatwillenableparticipants acquire skills for setting up and conducting HLA typing.