University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

San Cristobal de la Laguna, June 27th 2014

Location

4833 km fromUniversidad La Laguna

Doru PAMFIL visionary Rector

Domain: Life Sciences

Over 600 staff, 5500 students

Scientific and professional education

Budget:

€ 2.126.820 (gouvernmental resources) +

own resources (up to over € 18.000.000)

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Higher Education Institution and Research centre

Two pillars:

– Education: BSc 4y, MSc 2y, PhD 3y

–Research: Institute of Life Sciences

11 research centers, 25 research laboratories

Faculties:

– Faculty of Agriculture

– Faculty of Horticulture

– Faculty of Animal Science and Biotechnologies

– Faculty of Veterinary Medicine

– Faculty of Food Science and Technology

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Bachelor Programs (BSc) - 17

–Romanian students + international students (incoming)

Master Program (MSc) - 16

–Romanian, English, French

PhD Program (collaborations: bilateral diploma award)

–Romanian, English, French

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Atractive university

• Students from more than 14 countries

• Strongest international orientation

• Dynamic, open to changes and to explore new education blending methods

• Biggest University for Life Sciences in Romania

• Top publication index in our field in Romania

University of Agricultural Sciences and Veterinary Medicine Cluj-Napoca

Faculty of Food Science and Technology

FOOD TECHNOLOGY

FOOD ENGINEERING

SAFETY AND QUALITY CONTROL

FOOD SCIENCE

BIOCHEMISTRY FOOD

CHEMISTRY

NUTRITION FOOD

TOXICOLOGY

Faculty of Food Science and Technology

VISION

Be the recognized leader in providing integrated life science solutions for local and international customers.

MISSION

to produce and disseminate scientific knowledge

to develop innovations and know-how for the benefit of society

Faculty of Food Science and Technology

CAPABILITIES:

Laboratory of Food Quality and Safety Testing

Laboratory of Food Quality Control and Adulteration

Laboratory of Molecular Nutrition and Proteomics

Research laboratories

Equipments GC-FID, LC-MS, HPLC, FT-FIR & UV-VIS Spectrometry

cDNA gene expression, protein expression profile associated with bioactive compounds

Faculty of Food Science and Technology

3 BSc Programs

• Agro-Products Processing Technology

• Food Control and Expertise

• Food Engineering

4 MSc Programs

• Food Quality Management

• Processing Systems and Food Quality Control

• Food Safety and Consumer Protection

• GASTRONOMY, NUTRITION AND DIETETICS (in collaboration with University of Medicine and Pharmacy of Cluj-Napoca)

Disciplines Expertise and Competences Capabilities

Food Science food analysis and characterisation; foodingredient interaction;

Analysis of the chemical and physical properties ofFood, metabolomics

Food Law food regulation International research expertise

Food Production sustainable food production Novel feeding strategies

Food Consumer consumer attitudes, beliefs and perceptions of food andhealth

Novel quantitativemethodologies

Food Nutrition cellular and molecular nutrition, nutrigenomics, nutriproteomics

Genomic, transcriptomic, proteomic platforms

BiosystemsEngineering

food chain integrity, food process engineering

Traditional processing equipment for the meat , dairy, beer, bread and bakery industries

Food Safety identification and detection protocols for a range of food contaminants and food-borne pathogens

Analytical methods, microbiology methods

Pilot plants

Pilot plant for beer and wine Assist. Prof. dr. eng. Elena Mudura

Pilot plants

Pilot plant for bakery products Assist. Prof. dr. eng. Adriana Paucean

Pilot plants

Pilot plant for dairy products Assist. Prof. dr. eng. Mirela Jimborean

Main research topic field

• In vitro, in vivo and in silico study

– Cancer (prostate cancer, breast cancer) –• conducting studies on treatment/prevention of BAC (animal/vegetal) –

(AxonArray transcriptomics, microRNA, protein array, antibody array) Design of innovative food with bioactive anticancer compounds (colllaboration with prof H Kloker)

– Food proteins/peptides• Milk proteins and peptide with antihypertensive effect – design of

innovative yogurt

• Protein and peptide expression, purification and characterisation –specific milk bioactive peptides and proteins (collaboration with BOKU, prof D Haltrich)

• Microbiology – developing new biomarkers as bacterial membrane proteins for food quality and control

– Endemic plants (RT-PCR, proteomics) (prof. Pamfil)

• Human nutrition studies

BIOMARKER DISCOVERY AND VALIDATION

Motivation Biomarker Discovery and Validation

Overview of types of ‘omic’ biomarkers in terms of the informational complexity of the

models.

Azuaje F et al. Brief Bioinform 2009;10:367-377

© The Author 2009. Published by Oxford University Press. For Permissions, please email:

journals.permissions@oxfordjournals.org

IOCN 200822

Microarray experiments differential gene-expression

Simplified workflow of the literature mining system at GlaxoSmithKline.

Agarwal P , Searls D B Brief Bioinform 2008;9:479-492

© The Author 2008. Published by Oxford University Press. For Permissions, please email:

journals.permissions@oxfordjournals.org

Omics Matching Service

(gene&transcripts)-exp model PCa

Overlap (potential candidates)

MetaData pre-processing

Diff Gene-Express

Statistics Filtering Service

Filtering criteria: statistical meaningfulness

parametrized & addnotation(id, FC and p-Value )

List of meaningful genes diff express in PCa

GO

Biological functionMolecular function

Sub-cellular localization

Filterare (pebaza datelor

publice)

in silicoValidation

Yes! Where has this biomarker been reported?

No! This gene/transcripts

has not been reported as

biomarker before

Reported or not (search in public DBs)? –

YES/NO

Who reported this protein as biomarker (search

in public DB

-List of genes (GenBank)

-Link differentially expressed genes to

specific functional classes

List of exp. identified genes Public available

data(ArrayExpr ess;

GEO;CIBEX)

ANOVA, MatArray, t-test; IPA, PCA, etc

Computational pipeline of candidate

potential BMs in PCa

Suharoschi, Muntean, Iuga. 2012

Protein function prediction using SDS-PAGE and bioinformatic tools

National projects – lifescience

PN-II-RU-TE-2011-3-0154 -Nanobiotechnology tools for molecular medicine applications: Nano- and microstructures synthesis, characterisation and in vitro studies - Cristina Coman, PhD

PN-II-RU-PD-2012-3-0055 Brewer's yeast as biosorbentfor precious and heavy metals biosorption from waste waters - Andreea Stanila, PhD

Food and Health Cluj-Napoca inter-university platform

Babes Bolyai University

University of Medicine and

Pharmacy of Cluj-Napoca

Technical University of Cluj-Napoca

Colaborations

We are on facebook

USAMV INTERNATIONAL SYMPOSIUM

Contact

Prof. Sevastita MUSTE

DEAN – Faculty of Food Science and Technology

sevastita.muste@usamvcluj.ro

Dr. Ramona SUHAROSCHI

ramona.suharoschi@usamvcluj.ro

3-5 Calea Manastur Str., 400372 Cluj-Napoca, ROMANIA

Mobile:+40 728104320

Tel: 0264-596384

Fax: 0264-593792

www.ingineriealimentara.usamvcluj.ro

I hope to see you in Cluj-Napoca and in our university